FastQCFastQC Report
Thu 26 May 2016
SRR1051292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6767745
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT2151293.178739742706027No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT569500.8414915160071782No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT102930.15208906363936583No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA91030.13450565882727555No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT80840.11944894495877134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG645400.094.58431
TCGACGG652750.093.54842
CGACGGC663550.091.7960363
ACGGCGC673650.090.4919745
GACGGCG673750.090.485594
CGGCGCG679150.089.758546
TATCTCG334500.086.623882
ATATCTC338950.085.710911
ATCTCGA346450.083.5812453
TCTCGAG353350.081.379614
CTCGAGG360050.079.878455
AGGGCGC371600.077.493569
TCGAGGG374800.076.785786
GAGGGCG397650.072.345318
CGAGGGC396500.072.316097
CGCGCCG1045700.058.859719
GGCGCGC1053050.058.5440377
GCGCGCC1056600.058.3334248
ATCCATA716500.042.57372716-17
CCATATT721850.042.2779318-19
Produced by FastQC (version 0.11.5)