FastQCFastQC Report
Thu 26 May 2016
SRR1051292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6767745
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA235400.3478263439299205No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT114630.16937694904284956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG29200.085.7578351
TCGACGG34450.073.499532
CGACGGC47600.052.9913753
GACGGCG54500.046.282044
ACGGCGC57300.044.0197875
CGGCGCG64750.039.0257266
ATCTCGA43550.037.7514761
TCTCGAG49850.033.4494132
CTCGAGG56450.029.4524733
AGGGCGC61100.027.519547
CGCGCCG122100.026.6473549
GGGCGCG63950.026.0702828
TCGAGGG65450.025.329714
GGCGCGC130100.025.1548147
TATCTCG33250.024.7172992
GCGCGCC133250.024.5601588
ATATCTC41300.020.2492371
CGAGGGC85650.020.0210955
GAGGGCG86950.019.8297026
ATCCATA87100.016.96001216-17