##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051292_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6767745 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.63601081305516 34.0 31.0 34.0 31.0 34.0 2 32.894505629275336 34.0 31.0 34.0 31.0 34.0 3 32.99136817359401 34.0 31.0 34.0 31.0 34.0 4 36.39268988414901 37.0 37.0 37.0 35.0 37.0 5 36.30985136112545 37.0 37.0 37.0 35.0 37.0 6 36.30500070555259 37.0 37.0 37.0 35.0 37.0 7 36.30709490384168 37.0 37.0 37.0 35.0 37.0 8 36.303364562346836 37.0 37.0 37.0 35.0 37.0 9 38.09183103086774 39.0 39.0 39.0 37.0 39.0 10-11 38.08885197358943 39.0 39.0 39.0 37.0 39.0 12-13 38.06356437779496 39.0 38.5 39.0 36.0 39.0 14-15 39.597245759111786 41.0 40.0 41.0 37.0 41.0 16-17 39.53934131679016 41.0 40.0 41.0 37.0 41.0 18-19 39.527741958362796 41.0 39.5 41.0 37.0 41.0 20-21 39.50589627712037 41.0 39.5 41.0 37.0 41.0 22-23 39.44963928162187 41.0 39.0 41.0 36.0 41.0 24-25 39.39553706589122 41.0 39.0 41.0 36.0 41.0 26-27 39.30046241399462 41.0 39.0 41.0 36.0 41.0 28-29 39.167560686757554 40.5 39.0 41.0 35.5 41.0 30-31 39.03643806024016 40.0 38.5 41.0 35.0 41.0 32-33 38.9180679088825 40.0 38.0 41.0 35.0 41.0 34-35 38.79924524343042 40.0 38.0 41.0 35.0 41.0 36-37 38.6798667798506 40.0 38.0 41.0 35.0 41.0 38-39 38.53515897835985 40.0 38.0 41.0 34.5 41.0 40-41 38.61527133483901 40.0 38.0 41.0 35.0 41.0 42-43 38.631629368423305 40.0 38.0 41.0 35.0 41.0 44-45 38.567283932831394 40.0 38.0 41.0 34.5 41.0 46-47 38.4651020391578 40.0 38.0 41.0 34.0 41.0 48-49 38.39923741216609 40.0 38.0 41.0 34.0 41.0 50-51 38.36666400699199 40.0 37.5 41.0 34.0 41.0 52-53 38.18169900018396 40.0 37.0 41.0 34.0 41.0 54-55 37.969784174787904 40.0 37.0 41.0 34.0 41.0 56-57 37.70469240492956 40.0 36.0 41.0 33.0 41.0 58-59 37.45565228890864 39.0 35.5 41.0 33.0 41.0 60-61 37.142339213430766 39.0 35.0 41.0 33.0 41.0 62-63 36.804110748853574 38.5 35.0 41.0 32.0 41.0 64-65 36.475259188991316 37.5 35.0 40.0 32.0 41.0 66-67 36.08533411054938 37.0 35.0 40.0 32.0 41.0 68-69 35.70950708101443 36.5 35.0 39.0 31.0 41.0 70-71 35.36474120995989 36.0 35.0 39.0 31.0 41.0 72-73 34.96534384791389 35.5 35.0 38.5 31.0 40.0 74-75 34.587935346263784 35.0 34.0 37.0 30.5 39.5 76-77 33.71176440601707 35.0 33.5 36.5 29.5 39.0 78-79 33.910060884386155 35.0 34.0 36.0 30.0 39.0 80-81 33.728335509094975 35.0 34.0 36.0 30.0 37.5 82-83 33.484799811458615 35.0 34.0 36.0 30.0 37.0 84-85 33.248501605778586 35.0 34.0 35.0 30.0 37.0 86-87 33.05030730324503 35.0 34.0 35.0 29.0 36.0 88-89 32.847842080338424 35.0 34.0 35.0 29.0 36.0 90-91 32.69868353491451 35.0 34.0 35.0 29.0 36.0 92-93 32.54551929778678 35.0 34.0 35.0 29.0 36.0 94-95 32.387894933984654 35.0 34.0 35.0 29.0 35.0 96-97 32.20515873455634 35.0 33.0 35.0 29.0 35.0 98-99 31.994263081720725 35.0 33.0 35.0 27.0 35.0 100-101 31.555624214564823 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3641199759017866 1101 2 0.3115502410958726 1101 3 0.27292783217286143 1101 4 0.1971609191087893 1101 5 0.20049192099844504 1101 6 0.22208616397862357 1101 7 0.1812559617292493 1101 8 0.18447905507242268 1101 9 0.23611441627471663 1101 10-11 0.19482676394898846 1101 12-13 0.19357795787522036 1101 14-15 0.23539598420451568 1101 16-17 0.33412650033832136 1101 18-19 0.2759841038588249 1101 20-21 0.28721772768120957 1101 22-23 0.306133736303579 1101 24-25 0.27711554141038164 1101 26-27 0.22793714881427007 1101 28-29 0.31053820009309163 1101 30-31 0.23755464121729375 1101 32-33 0.311036222568255 1101 34-35 0.2859924997910852 1101 36-37 0.17485379742137752 1101 38-39 0.19116227386830786 1101 40-41 0.3126179388089554 1101 42-43 0.2668674729500182 1101 44-45 0.3324449276211041 1101 46-47 0.31253968131091625 1101 48-49 0.32357455539694513 1101 50-51 0.2964325651282209 1101 52-53 0.26545465827460646 1101 54-55 0.26817717682538955 1101 56-57 0.296188930391196 1101 58-59 0.2814852515275845 1101 60-61 0.24758985836492542 1101 62-63 0.12590475398497603 1101 64-65 0.1379900286397202 1101 66-67 0.26576814759372525 1101 68-69 0.24734245752500783 1101 70-71 0.20572968094025867 1101 72-73 0.2211732661757182 1101 74-75 0.24471685614602023 1101 76-77 0.1172549040616957 1101 78-79 0.1674898161703524 1101 80-81 0.17222984004828135 1101 82-83 0.17583170205804066 1101 84-85 0.13088305044093573 1101 86-87 -0.006565617796873369 1101 88-89 -0.030573158999310124 1101 90-91 -0.002951381875114123 1101 92-93 -0.04361423239335238 1101 94-95 -0.07334169932240542 1101 96-97 -0.10156856538601744 1101 98-99 -0.09799176797346476 1101 100-101 -0.1283077406599915 1102 1 0.29342690767199997 1102 2 0.20489134530109254 1102 3 0.184140576454503 1102 4 0.15128970893669447 1102 5 0.12806708453479843 1102 6 0.1526685882545351 1102 7 0.09225765567211397 1102 8 0.13284623747840385 1102 9 0.18811998407741015 1102 10-11 0.1491975312430398 1102 12-13 0.16775597659045616 1102 14-15 0.1736098711730918 1102 16-17 0.10181198662905899 1102 18-19 0.22464011789778482 1102 20-21 0.16913222120160754 1102 22-23 0.17146739491786178 1102 24-25 0.1388822473819431 1102 26-27 0.1523403668134904 1102 28-29 0.1941651826924371 1102 30-31 0.22572857549661762 1102 32-33 0.18974067212463552 1102 34-35 0.17206500331064234 1102 36-37 0.23640106953197204 1102 38-39 0.25029813543825696 1102 40-41 0.25926165243090793 1102 42-43 0.20598682630345166 1102 44-45 0.16224433006215833 1102 46-47 0.1520135117120205 1102 48-49 0.20661936212902532 1102 50-51 0.2325622015574922 1102 52-53 0.23258301291672723 1102 54-55 0.18828856607704836 1102 56-57 0.2502566492024769 1102 58-59 0.15862533742409113 1102 60-61 0.20656095322065227 1102 62-63 0.26659638098476535 1102 64-65 0.17777069652784405 1102 66-67 0.14905111260789283 1102 68-69 0.17940473837865767 1102 70-71 0.2792625909642865 1102 72-73 0.3254417407744157 1102 74-75 0.2650957707980055 1102 76-77 0.23613895773662108 1102 78-79 0.3327111174793913 1102 80-81 0.27740283611611716 1102 82-83 0.27917150414786107 1102 84-85 0.29471321665031525 1102 86-87 0.30653599216633864 1102 88-89 0.255359630647483 1102 90-91 0.21931990291011516 1102 92-93 0.3714372661240546 1102 94-95 0.23866722160173737 1102 96-97 0.22501455952154714 1102 98-99 0.2492368743462663 1102 100-101 0.3052841072424073 1103 1 0.30005635245620965 1103 2 0.195233720493178 1103 3 0.2054889015549506 1103 4 0.1178798463928672 1103 5 0.14013892838239173 1103 6 0.09940476059588121 1103 7 0.06441956501509338 1103 8 0.11147814803862843 1103 9 0.09217558969853457 1103 10-11 0.18139572374554547 1103 12-13 0.1766283744284891 1103 14-15 0.20969964836366728 1103 16-17 0.2104994824596318 1103 18-19 0.19055521707131362 1103 20-21 0.21895294248557207 1103 22-23 0.23808065814834833 1103 24-25 0.22707672176802873 1103 26-27 0.2409341270206582 1103 28-29 0.16514191169889614 1103 30-31 0.2123189391696343 1103 32-33 0.2361093968244603 1103 34-35 0.32427189923461697 1103 36-37 0.18274073229307675 1103 38-39 0.197152978038595 1103 40-41 0.24445556695838633 1103 42-43 0.24186642958449767 1103 44-45 0.21631887307715658 1103 46-47 0.16304971478479047 1103 48-49 0.18742592397951796 1103 50-51 0.23175155794669422 1103 52-53 0.24114021695695698 1103 54-55 0.2554386419897696 1103 56-57 0.3024592229249734 1103 58-59 0.271442984373806 1103 60-61 0.30601656545609757 1103 62-63 0.27478151572231724 1103 64-65 0.28289155574356073 1103 66-67 0.2352517407283301 1103 68-69 0.244108110114432 1103 70-71 0.29326390348361286 1103 72-73 0.28787924579933843 1103 74-75 0.3239175484073016 1103 76-77 0.2937881198387444 1103 78-79 0.3570219847834579 1103 80-81 0.4169606453331838 1103 82-83 0.45726796581516993 1103 84-85 0.4318773697262017 1103 86-87 0.4260504155392084 1103 88-89 0.43857613550287766 1103 90-91 0.41553726290383963 1103 92-93 0.4477916753407243 1103 94-95 0.45599636785645004 1103 96-97 0.4594675873886942 1103 98-99 0.45770277499963186 1103 100-101 0.4739947817518342 1104 1 0.19158984078705288 1104 2 0.13584706746750186 1104 3 0.10465272487950017 1104 4 0.07416520871571208 1104 5 0.07003381658484642 1104 6 0.0634270246481563 1104 7 0.07141278316736077 1104 8 0.016110305720900442 1104 9 0.06592022697522992 1104 10-11 0.11313027619338101 1104 12-13 0.07875230432201619 1104 14-15 0.04872092804490791 1104 16-17 0.14072019118047052 1104 18-19 0.10224812901491731 1104 20-21 0.1458503138216969 1104 22-23 0.17153383708193104 1104 24-25 0.17237918141951525 1104 26-27 0.155006975291208 1104 28-29 0.16413621051845695 1104 30-31 0.061523759064435524 1104 32-33 0.1143363798857493 1104 34-35 0.1990549577094498 1104 36-37 0.1758437208435666 1104 38-39 0.185449943934195 1104 40-41 0.1292838947474806 1104 42-43 0.1113219320535066 1104 44-45 0.17515834453413248 1104 46-47 0.10829059072866443 1104 48-49 0.14369103358528434 1104 50-51 0.07953994976761436 1104 52-53 0.0942768792526536 1104 54-55 0.11307053714511284 1104 56-57 0.18188698194209962 1104 58-59 0.19872900526676318 1104 60-61 0.2681461757372361 1104 62-63 0.34362444416166227 1104 64-65 0.3073236953867422 1104 66-67 0.2824282049722129 1104 68-69 0.3098833521934523 1104 70-71 0.28927468071639595 1104 72-73 0.32411646391275184 1104 74-75 0.35556822432669577 1104 76-77 0.28795936715396664 1104 78-79 0.3439900399771858 1104 80-81 0.38217499601056204 1104 82-83 0.3380451009369523 1104 84-85 0.4855036681130045 1104 86-87 0.516312208423237 1104 88-89 0.526970407910234 1104 90-91 0.46863711098187366 1104 92-93 0.4962052736032092 1104 94-95 0.44888092258366186 1104 96-97 0.499362121021683 1104 98-99 0.4841932365911532 1104 100-101 0.5931544118096639 1105 1 0.023292531732600708 1105 2 0.05574565240442553 1105 3 0.002315840331924335 1105 4 0.014627216025402845 1105 5 -0.013562200560279791 1105 6 -0.05051347353406044 1105 7 -0.05866249486246744 1105 8 -0.05255983865336589 1105 9 -0.0632099484646389 1105 10-11 -0.028459925491972626 1105 12-13 -0.03325054677160466 1105 14-15 -0.02245106905821359 1105 16-17 -0.006537685779051117 1105 18-19 -0.06518445274314644 1105 20-21 -0.050592507049842084 1105 22-23 -0.09066989199428122 1105 24-25 -0.041919438704191236 1105 26-27 -0.03446801859328019 1105 28-29 -0.05741582796527922 1105 30-31 0.0018689872181951728 1105 32-33 0.01000654956558833 1105 34-35 0.011007065120722359 1105 36-37 0.04489396684980562 1105 38-39 0.08995914250695591 1105 40-41 0.032406926787942325 1105 42-43 -0.05337211764420857 1105 44-45 -0.11613332378944818 1105 46-47 -0.08613436441421385 1105 48-49 0.003705216751782814 1105 50-51 -0.03276019351007875 1105 52-53 0.0034245757835051904 1105 54-55 -0.008630165506062326 1105 56-57 -0.023641440424647442 1105 58-59 -0.027961761937710605 1105 60-61 0.07906618966735124 1105 62-63 0.08348636013031552 1105 64-65 0.13910535174440497 1105 66-67 0.13770301737964985 1105 68-69 0.17268617243838236 1105 70-71 0.1932112384606839 1105 72-73 0.2842506677575116 1105 74-75 0.28557938287569584 1105 76-77 0.27028444243802596 1105 78-79 0.2580496550675022 1105 80-81 0.2460042340600097 1105 82-83 0.3260207280630709 1105 84-85 0.33812989166718666 1105 86-87 0.33204646143308736 1105 88-89 0.3946217140991308 1105 90-91 0.3768308600253789 1105 92-93 0.39446722716870397 1105 94-95 0.358507050940716 1105 96-97 0.36764078831014047 1105 98-99 0.38315617839919014 1105 100-101 0.40469602681080374 1106 1 -0.13148150740222775 1106 2 -0.11332689905347593 1106 3 -0.14879285609053028 1106 4 -0.06073995350767092 1106 5 -0.0479608193508696 1106 6 -0.06775578754069755 1106 7 -0.043444329242198876 1106 8 -0.1665290187522075 1106 9 -0.11219760430626025 1106 10-11 -0.192057962507576 1106 12-13 -0.09421187172701906 1106 14-15 -0.16474839690899756 1106 16-17 -0.166945839402743 1106 18-19 -0.16529030862560035 1106 20-21 -0.16651338784502911 1106 22-23 -0.16433677013368708 1106 24-25 -0.11370078714656984 1106 26-27 -0.14674257656746903 1106 28-29 -0.13163156642975338 1106 30-31 -0.11670635163029885 1106 32-33 -0.11317138786660763 1106 34-35 -0.10893738110474516 1106 36-37 -0.10755174483750096 1106 38-39 -0.09018796321309708 1106 40-41 -0.057422645030392516 1106 42-43 -0.07822765252117847 1106 44-45 -0.18132669479417274 1106 46-47 -0.15536943491731137 1106 48-49 -0.12885259376302827 1106 50-51 -0.12202024844799553 1106 52-53 -0.07012515855387846 1106 54-55 -0.019495024305832942 1106 56-57 0.01108032248179569 1106 58-59 -0.0844376532024782 1106 60-61 -0.05205373140157832 1106 62-63 -0.10487867583410804 1106 64-65 -0.017488311835109016 1106 66-67 0.02664544561530846 1106 68-69 0.026796738369789352 1106 70-71 0.07148708045681218 1106 72-73 0.10722298873263014 1106 74-75 0.13530053414225307 1106 76-77 0.08670491912906186 1106 78-79 0.22885998089861914 1106 80-81 0.23015328268579793 1106 82-83 0.2467680929497149 1106 84-85 0.3103697000593897 1106 86-87 0.3166709356336952 1106 88-89 0.3054804607182362 1106 90-91 0.24683554645237393 1106 92-93 0.28706872142603856 1106 94-95 0.363516727505548 1106 96-97 0.37188460315451266 1106 98-99 0.3897349685839373 1106 100-101 0.5062295050254519 1107 1 -0.20250191502313442 1107 2 -0.1644704602926197 1107 3 -0.18144744440842686 1107 4 -0.10958555882629639 1107 5 -0.17815619795378268 1107 6 -0.18667862813920522 1107 7 -0.18623040374951927 1107 8 -0.1806930151655166 1107 9 -0.28530262402822615 1107 10-11 -0.2182934728358603 1107 12-13 -0.21832967472754206 1107 14-15 -0.2868045353698321 1107 16-17 -0.2436622351279638 1107 18-19 -0.27538744105788737 1107 20-21 -0.2980508769008594 1107 22-23 -0.28231300950666594 1107 24-25 -0.2973676799210665 1107 26-27 -0.30186833583246653 1107 28-29 -0.3332834739250714 1107 30-31 -0.39992873343947366 1107 32-33 -0.3059925240544388 1107 34-35 -0.36227049358431174 1107 36-37 -0.2648369873095717 1107 38-39 -0.3890853912717418 1107 40-41 -0.4890411993308206 1107 42-43 -0.31032784443485895 1107 44-45 -0.33079627614402085 1107 46-47 -0.31679676605627805 1107 48-49 -0.363067040446154 1107 50-51 -0.22611072362082751 1107 52-53 -0.22894499866919205 1107 54-55 -0.22351086511077511 1107 56-57 -0.22651799409933204 1107 58-59 -0.1959832373276882 1107 60-61 -0.1909499120793825 1107 62-63 -0.1501592607660669 1107 64-65 -0.21809891903510703 1107 66-67 -0.07920667815785976 1107 68-69 -0.08882785134775162 1107 70-71 -0.07889909746995016 1107 72-73 -0.045084109098013414 1107 74-75 -0.06404971744470345 1107 76-77 -0.14743655493510488 1107 78-79 -0.05528643182837811 1107 80-81 -0.07976314065989243 1107 82-83 -0.07793095790450622 1107 84-85 0.019020583227010945 1107 86-87 0.15222660996560933 1107 88-89 0.07990584458682548 1107 90-91 0.03388103521030672 1107 92-93 0.11913154231693568 1107 94-95 0.06765497719639768 1107 96-97 0.03943041213513254 1107 98-99 0.035920587344399735 1107 100-101 0.0946858438247773 1108 1 -0.35733661732287914 1108 2 -0.3428901487062106 1108 3 -0.3791414466304701 1108 4 -0.21511259561275153 1108 5 -0.33787727855197147 1108 6 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0.10777263835240092 2304 94-95 0.17011278266854646 2304 96-97 0.11653244379016314 2304 98-99 0.07605787819949938 2304 100-101 0.09448916356118886 2305 1 -0.3896906452846025 2305 2 -0.39094342411527094 2305 3 -0.3656892401587939 2305 4 -0.3870305775806031 2305 5 -0.30171154014846024 2305 6 -0.12241769834577099 2305 7 -0.06759444359827427 2305 8 -0.08284314499817214 2305 9 -0.07077476701145002 2305 10-11 -0.05601156557096232 2305 12-13 -0.07546443013862358 2305 14-15 -0.13043248122861684 2305 16-17 -0.30870488457734524 2305 18-19 -0.22154895810643183 2305 20-21 -0.1273281878105621 2305 22-23 -0.08489329088604336 2305 24-25 -0.07993602023137214 2305 26-27 -0.27779425158802695 2305 28-29 -0.2325153610723376 2305 30-31 -0.37685367478505327 2305 32-33 -0.18105247192018226 2305 34-35 -0.09903374135047471 2305 36-37 -0.1639039905585591 2305 38-39 -0.16775870503200707 2305 40-41 -0.1075412240447946 2305 42-43 -0.20598613218162853 2305 44-45 -0.0851878620445703 2305 46-47 -0.04588417198212369 2305 48-49 -0.11965212668835079 2305 50-51 -0.11952297778153564 2305 52-53 -0.17754618918662857 2305 54-55 -0.1220671042458008 2305 56-57 -0.08344172686377505 2305 58-59 -0.15839704828942303 2305 60-61 -0.16883269760501207 2305 62-63 -0.11115749283975163 2305 64-65 -0.0441789690919876 2305 66-67 -0.00848187524820787 2305 68-69 -0.07464729288438576 2305 70-71 -0.08941633255727055 2305 72-73 -0.011883594794042551 2305 74-75 -0.0803350274882817 2305 76-77 0.035216454490957005 2305 78-79 -0.0687484970174097 2305 80-81 -0.06358241377532892 2305 82-83 -0.08014159720646319 2305 84-85 -0.022797945235460304 2305 86-87 -0.008664462330727929 2305 88-89 -0.025701207976858598 2305 90-91 -0.08121321247556068 2305 92-93 -0.07347061431261181 2305 94-95 -0.05307676085431723 2305 96-97 -0.08959817383708923 2305 98-99 -0.05143482839510938 2305 100-101 -0.031438665730206594 2306 1 -0.3409696797606614 2306 2 -0.5383402483102202 2306 3 -0.3643517823805382 2306 4 -0.1629533507504135 2306 5 -0.12140145739401476 2306 6 -0.12724315955268395 2306 7 -0.414353915148169 2306 8 -0.23036364212222082 2306 9 -0.17948280393441252 2306 10-11 -0.1640214828905684 2306 12-13 -0.3735697863965157 2306 14-15 -0.22361919767823935 2306 16-17 -0.19801469686121465 2306 18-19 -0.5308273537047583 2306 20-21 -0.3487254292834905 2306 22-23 -0.30313354233646805 2306 24-25 -0.21449757553027382 2306 26-27 -0.2508717726664358 2306 28-29 -0.16171636096080988 2306 30-31 -0.1721581963505585 2306 32-33 -0.36300873920007604 2306 34-35 -0.2382503263083251 2306 36-37 -0.20325751860635677 2306 38-39 -0.19842322800858625 2306 40-41 -0.5666264312432432 2306 42-43 -0.34568702319484856 2306 44-45 -0.25218079146315375 2306 46-47 -0.213748870815742 2306 48-49 -0.20223254466699103 2306 50-51 -0.1762398815204662 2306 52-53 -0.1897403341490147 2306 54-55 -0.19242216180936111 2306 56-57 -0.26390929258810303 2306 58-59 -0.3042787548549981 2306 60-61 -0.32782729886408646 2306 62-63 -0.24505790080645085 2306 64-65 -0.23169804540498973 2306 66-67 -0.21424683801842548 2306 68-69 -0.20329524840500568 2306 70-71 -0.2016944715228206 2306 72-73 -0.18067910271182086 2306 74-75 -0.1691181481360431 2306 76-77 -0.13302140457871303 2306 78-79 -0.3303946990837261 2306 80-81 -0.22054005874495175 2306 82-83 -0.32673698603143464 2306 84-85 -0.39290845373622574 2306 86-87 -0.26361372802332284 2306 88-89 -0.2181447637484908 2306 90-91 -0.1796223694176433 2306 92-93 -0.2542462538737027 2306 94-95 -0.25694272877039737 2306 96-97 -0.32235921168985726 2306 98-99 -0.3517410068426443 2306 100-101 -0.3275067026999423 2307 1 -0.6235861010262269 2307 2 -0.8001080229628528 2307 3 -0.5376843380216059 2307 4 -0.3028224431601103 2307 5 -0.47738054579198774 2307 6 -0.4907572233575621 2307 7 -0.30605636124212765 2307 8 -0.2396013081845325 2307 9 -0.28743333456159803 2307 10-11 -0.352414944731855 2307 12-13 -0.3227496111587129 2307 14-15 -0.8318798812640509 2307 16-17 -0.8083628623755814 2307 18-19 -0.4868059538556224 2307 20-21 -0.4545827744676103 2307 22-23 -0.39114985408141933 2307 24-25 -0.46565478797029414 2307 26-27 -0.495526531927446 2307 28-29 -0.457312593120605 2307 30-31 -0.41406065188980534 2307 32-33 -0.4150041909264175 2307 34-35 -0.3906746472939773 2307 36-37 -0.4220966867882794 2307 38-39 -0.3703238599558105 2307 40-41 -0.32620502625327674 2307 42-43 -0.37961713462707536 2307 44-45 -0.3579215068914223 2307 46-47 -0.4124750340934966 2307 48-49 -0.35569591243839227 2307 50-51 -0.36847987017211636 2307 52-53 -0.31197845541966274 2307 54-55 -0.37295999468797447 2307 56-57 -0.4025538108768174 2307 58-59 -0.37376629878438195 2307 60-61 -0.31672629133004193 2307 62-63 -0.3367463169115723 2307 64-65 -0.3522336161449573 2307 66-67 -0.32809062079242324 2307 68-69 -0.2564450016989923 2307 70-71 -0.3353350677421716 2307 72-73 -0.32028630418599136 2307 74-75 -0.2901831517148068 2307 76-77 -0.259983964760913 2307 78-79 -0.4692211452536199 2307 80-81 -0.46138394199276433 2307 82-83 -0.43236762910346016 2307 84-85 -0.3491148244502611 2307 86-87 -0.30394185810934715 2307 88-89 -0.25504310016795984 2307 90-91 -0.35990031470650763 2307 92-93 -0.3419288191581984 2307 94-95 -0.29536421008455704 2307 96-97 -0.2994648184241484 2307 98-99 -0.2627665834816426 2307 100-101 -0.388635587392681 2308 1 -0.9609914327426772 2308 2 -0.7252058635623015 2308 3 -0.6211489509823522 2308 4 -0.6505254162489535 2308 5 -0.5533809186118575 2308 6 -0.5103335485814853 2308 7 -0.4787807439569107 2308 8 -0.6971933763693201 2308 9 -0.6883522564606324 2308 10-11 -0.606156813884617 2308 12-13 -0.7532744886223526 2308 14-15 -0.7647619337938991 2308 16-17 -0.7590521871842597 2308 18-19 -0.6400116242440035 2308 20-21 -0.6769740736499728 2308 22-23 -0.6986968398685818 2308 24-25 -0.6329832282222299 2308 26-27 -0.6786944773935844 2308 28-29 -0.7309917897757643 2308 30-31 -0.702039014018176 2308 32-33 -0.6298123470380759 2308 34-35 -0.725499718073003 2308 36-37 -0.958882497049153 2308 38-39 -0.831938568574337 2308 40-41 -0.8415717572673174 2308 42-43 -0.8435409445927178 2308 44-45 -0.7771887054881788 2308 46-47 -0.737459700994819 2308 48-49 -0.7472817834660503 2308 50-51 -0.6994368432222373 2308 52-53 -0.7231983218152891 2308 54-55 -0.6413445103409288 2308 56-57 -0.7251176693887302 2308 58-59 -0.6772606355121198 2308 60-61 -0.8466133101328737 2308 62-63 -0.9420728987277514 2308 64-65 -0.7444882391086551 2308 66-67 -0.8663127023459509 2308 68-69 -0.8349276217815245 2308 70-71 -1.1830929039873723 2308 72-73 -0.9813005086428177 2308 74-75 -0.8690581623426183 2308 76-77 -0.8683310408846125 2308 78-79 -0.9721818436629945 2308 80-81 -0.9352749606938957 2308 82-83 -1.0255795192117247 2308 84-85 -1.035215241975088 2308 86-87 -1.0870078314947023 2308 88-89 -1.0433979726570257 2308 90-91 -0.985175541662052 2308 92-93 -0.8728967171341537 2308 94-95 -0.8904108797755192 2308 96-97 -0.8797562873241276 2308 98-99 -1.0472400961313824 2308 100-101 -1.4396223109248325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 6.0 11 48.0 12 165.0 13 508.0 14 1445.0 15 2895.0 16 5069.0 17 8406.0 18 14645.0 19 25629.0 20 23572.0 21 20712.0 22 19663.0 23 20730.0 24 23485.0 25 28052.0 26 34740.0 27 43101.0 28 53481.0 29 66531.0 30 83872.0 31 105913.0 32 136141.0 33 182183.0 34 263632.0 35 437668.0 36 835297.0 37 1733713.0 38 2101892.0 39 494131.0 40 420.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.40435374020973 28.939414271551893 24.89598623317646 22.760245755061913 2 24.154241871641737 28.31094848295126 26.56751458341245 20.967295061994555 3 23.081881055835368 26.800810798909993 26.806529183116258 23.310778962138386 4 24.2641981558574 26.63824250116107 26.50816455930944 22.58939478367209 5 24.191252765816092 27.329351326542582 25.76809671955814 22.711299188083178 6 24.029894743374637 26.609424557219576 25.316364608891146 24.044316090514638 7 24.364023762715643 26.79261703861478 25.68996024525156 23.153398953418012 8 23.780151291161236 26.634854002330172 25.99724132632066 23.587753380187937 9 23.18114822588617 26.297607253228367 26.35247043143617 24.16877408944929 10-11 23.611092025482638 26.661221721563088 26.11835256795284 23.60933368500143 12-13 23.433795841797515 26.62359413759292 26.276151301499528 23.666458719110036 14-15 23.324549018912503 26.661864476276808 26.357686348998076 23.65590015581261 16-17 23.436505263310323 26.784224926475602 26.28261812831231 23.496651681901763 18-19 23.139716404799533 26.630857102328765 26.540509431132524 23.688917061739176 20-21 22.984590879236734 26.697991723978966 26.617669548719697 23.699747848064607 22-23 23.002351595693987 26.610680514706154 26.965096941448003 23.421870948151856 24-25 22.980690023043127 26.301936612564454 27.353069597037123 23.364303767355302 26-27 23.00644453950319 26.33751714936068 27.416103886892902 23.23993442424323 28-29 23.03759228517032 26.426956098375452 27.4118631833794 23.123588433074826 30-31 23.004899133062086 26.359340978242624 27.380680783861287 23.255079104834 32-33 23.006584911222276 26.40573041685229 27.353956155262942 23.23372851666249 34-35 23.072522679267614 26.45022825180322 27.357768355633965 23.119480713295196 36-37 23.06282964266532 26.392601967124946 27.26685180957616 23.27771658063358 38-39 23.066242891834726 26.467390541458048 27.20697957739247 23.259386989314756 40-41 23.124433936663852 26.631956119106338 27.04652190073605 23.19708804349376 42-43 23.168568286774697 26.573383898726576 26.952942031739408 23.30510578275932 44-45 23.05632045172739 26.66443446933313 26.994624329980628 23.284620748958847 46-47 23.149243901959704 26.778789329975428 26.847719619067433 23.22424714899744 48-49 23.117279093701075 26.73554485282764 26.829091521622043 23.318084531849234 50-51 23.065651853017513 26.73438493914886 26.885011181715623 23.314952026118004 52-53 23.127976896292633 26.85316157745305 26.79433843916992 23.224523087084396 54-55 23.130760509529104 26.771208227740463 26.756845971749204 23.34118529098123 56-57 23.096619455692863 26.792180227332857 26.793354917589568 23.317845399384712 58-59 23.20213205857827 26.89419637880138 26.70954809599051 23.194123466629847 60-61 23.17013273993036 26.816923509972675 26.658539882929986 23.35440386716698 62-63 23.148751910717678 26.901249973218555 26.6719934040068 23.278004712056973 64-65 23.186452799270658 26.981476104670023 26.592742486603736 23.239328609455587 66-67 23.15079099463706 26.91463700242843 26.588080667932967 23.34649133500154 68-69 23.145848498726718 26.974261786267302 26.568446756623352 23.311442958382628 70-71 23.205513270820123 27.11095116939281 26.50614835699287 23.177387202794193 72-73 23.162356657173586 27.03848750225432 26.455448754392368 23.343707086179723 74-75 23.101585124325847 27.13295381646732 26.511282109515438 23.254178949691394 76-77 23.166782080136127 27.18047418349803 26.44270469280057 23.210039043565267 78-79 23.17212462908187 27.1522929766333 26.429384960782638 23.246197433502186 80-81 23.133701305767982 27.168269407302546 26.526611656243144 23.171417630686328 82-83 23.151144525182037 27.30495301164746 26.439309660343092 23.104592802827415 84-85 23.100448702026657 27.234655630392584 26.45727682035207 23.207618847228684 86-87 23.06659408697122 27.330052766561565 26.490724441995567 23.112628704471643 88-89 23.13289816877442 27.417738767689148 26.384316521930316 23.06504654160611 90-91 23.081646292243015 27.338554493339483 26.381134140213014 23.19866507420449 92-93 23.07369903334387 27.404472151378762 26.410044239811103 23.111784575466263 94-95 23.148813510884455 27.48392551766165 26.340333212016485 23.02692775943741 96-97 23.0405536742022 27.509294284520173 26.29131654026913 23.158835501008497 98-99 23.00991723215594 27.585173585363165 26.30835940522761 23.09654977725329 100-101 23.101421114653327 27.690960203617987 26.205051292375774 23.002567389352908 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 21937.0 1 12501.0 2 2153.5 3 1047.5 4 819.5 5 742.5 6 676.5 7 629.5 8 618.0 9 641.0 10 708.0 11 788.5 12 966.5 13 1429.5 14 1905.5 15 2318.5 16 5418.0 17 5597.5 18 2946.5 19 3220.5 20 3939.0 21 5192.0 22 7089.0 23 9746.0 24 13325.5 25 17936.5 26 23265.5 27 29921.0 28 37751.5 29 46025.0 30 55068.0 31 65806.5 32 79935.5 33 94023.5 34 104615.5 35 119139.5 36 137582.5 37 157904.0 38 177403.0 39 192473.0 40 206318.0 41 223076.0 42 245894.0 43 266089.0 44 279501.5 45 285772.5 46 286854.5 47 287158.0 48 289254.5 49 288525.5 50 280583.5 51 268467.0 52 245173.5 53 216702.5 54 198677.0 55 187229.5 56 169953.0 57 149592.0 58 136321.5 59 125846.5 60 111848.5 61 98952.0 62 87063.5 63 75585.0 64 66205.0 65 55843.0 66 45972.5 67 36695.5 68 28154.0 69 21597.0 70 16117.5 71 12200.0 72 9554.5 73 7734.5 74 6390.5 75 4804.5 76 3560.0 77 2835.5 78 2197.5 79 1442.5 80 742.5 81 412.0 82 254.0 83 170.5 84 94.5 85 43.5 86 21.5 87 8.0 88 4.5 89 3.0 90 1.5 91 2.5 92 2.0 93 0.5 94 1.0 95 0.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01969636858362719 2 0.00437368724737708 3 0.0014628210726024694 4 0.003900856193606585 5 0.004477119040389377 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.9208761559426368E-4 14-15 0.0 16-17 0.0013815532352356656 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.511914973155756E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 9.087221814651705E-4 42-43 1.0343179301229582E-4 44-45 0.0018248323481455048 46-47 5.614868763524631E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 8.865582258196786E-5 56-57 5.171589650614791E-5 58-59 1.9947560080942764E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 9.604380779713184E-5 70-71 2.955194086065595E-5 72-73 0.0027261665443955112 74-75 0.0015588648803996012 76-77 7.166345658709068E-4 78-79 0.0011673016639959098 80-81 0.001750952495993865 82-83 5.91038817213119E-5 84-85 2.955194086065595E-5 86-87 1.773116451639357E-4 88-89 5.46710905922135E-4 90-91 4.137271720491833E-4 92-93 0.0012559574865778779 94-95 0.005053381887172167 96-97 1.5514768951844374E-4 98-99 0.001189465619641402 100-101 0.0025119149731557557 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6767745.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.08302972965973 #Duplication Level Percentage of deduplicated Percentage of total 1 81.93082004163692 40.21412875879066 2 9.964010283033566 9.781276258975433 3 2.5826132627210123 3.802874506630468 4 1.1082056551263477 2.1757636446857425 5 0.625516018841055 1.5351110674576947 6 0.4067845970800045 1.1979732283227307 7 0.31280662457744046 1.0747447797638312 8 0.2616000283589021 1.0272097575375856 9 0.21564415427196645 0.9526021591642432 >10 2.2437972026745627 22.703744530740366 >50 0.26154173336587017 8.936252221442658 >100 0.08626851547716832 5.675448795828427 >500 1.2057949495872415E-4 0.051090631107755434 >1k 1.8086889345180144E-4 0.2583231682981531 >5k 3.0144815575300225E-5 0.0955524956108295 >10k+ 6.028963115060045E-5 0.517903995643522 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23540 0.3478263439299205 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11463 0.16937694904284956 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.728310537704952E-4 0.0 0.0 0.0 0.0 2 4.728310537704952E-4 0.0 0.0 0.0 0.0 3 4.728310537704952E-4 0.0 0.0 0.0 0.0 4 4.728310537704952E-4 0.0 0.0 0.0 0.0 5 4.728310537704952E-4 2.955194086065595E-5 0.0 0.0 0.0 6 5.023829946311511E-4 7.387985215163988E-5 0.0 0.0 0.0 7 5.319349354918071E-4 7.387985215163988E-5 0.0 0.0 0.0 8 5.467109059221351E-4 7.387985215163988E-5 0.0 0.0 0.0 9 5.614868763524631E-4 7.387985215163988E-5 0.0 0.0 0.0 10-11 5.91038817213119E-4 8.865582258196784E-5 0.0 0.0 0.0 12-13 6.353667285041029E-4 1.0343179301229582E-4 0.0 0.0 0.0 14-15 8.496182997438585E-4 1.255957486577878E-4 0.0 0.0 0.0 16-17 0.001182077634426238 1.4775970430327975E-4 0.0 0.0 0.0 18-19 0.00120424159007173 1.7731164516393569E-4 0.0 0.0 0.0 20-21 0.0012928974126536978 2.0686358602459165E-4 0.0 0.0 0.0 22-23 0.0014184931613114855 2.2163955645491961E-4 0.0 0.0 0.0 24-25 0.0015367009247541094 2.2163955645491961E-4 0.0 0.0 0.0 26-27 0.001758340481209029 2.3641552688524758E-4 0.0 0.0 0.0 28-29 0.002016919963739769 2.659674677459035E-4 0.0 0.0 0.0 30-31 0.002438035121004116 2.8074343817623154E-4 0.0 0.0 0.0 32-33 0.002785270426116823 2.955194086065595E-4 0.0 7.387985215163988E-6 0.0 34-35 0.003088177819938547 3.620112755430354E-4 0.0 1.4775970430327976E-5 0.0 36-37 0.00342802513983609 3.693992607581994E-4 0.0 1.4775970430327976E-5 0.0 38-39 0.003826976341454945 4.063391868340193E-4 0.0 1.4775970430327976E-5 0.0 40-41 0.004418015158668065 4.2850314247951126E-4 0.0 1.4775970430327976E-5 0.0 42-43 0.005016441961096347 4.2850314247951126E-4 0.0 1.4775970430327976E-5 0.0 44-45 0.0058217323495492225 4.580550833401672E-4 0.0 1.4775970430327976E-5 0.0 46-47 0.006619634752786933 4.580550833401672E-4 0.0 1.4775970430327976E-5 0.0 48-49 0.007580072830758251 4.580550833401672E-4 0.0 1.4775970430327976E-5 0.0 50-51 0.008570062849590226 4.580550833401672E-4 0.0 1.4775970430327976E-5 0.0 52-53 0.009220205548524657 4.7283105377049516E-4 0.0 1.4775970430327976E-5 0.0 54-55 0.010018107951762367 5.24546950276643E-4 0.0 1.4775970430327976E-5 0.0 56-57 0.01100809797059434 5.319349354918071E-4 0.0 1.4775970430327976E-5 0.0 58-59 0.012057191871147628 5.467109059221351E-4 0.0 1.4775970430327976E-5 0.0 60-61 0.013254045476004193 5.540988911372991E-4 0.0 1.4775970430327976E-5 0.0 62-63 0.014798134385973466 5.91038817213119E-4 0.0 1.4775970430327976E-5 0.0 64-65 0.016704234571485774 6.13202772858611E-4 0.0 1.4775970430327976E-5 0.0 66-67 0.019297417382008335 6.205907580737749E-4 0.0 1.4775970430327976E-5 0.0 68-69 0.022481639009744016 6.427547137192669E-4 0.0 1.4775970430327976E-5 0.0 70-71 0.02805217986197766 6.649186693647588E-4 0.0 1.4775970430327976E-5 0.0 72-73 0.0341989835609941 6.649186693647588E-4 0.0 1.4775970430327976E-5 0.0 74-75 0.041586968776158084 6.723066545799228E-4 0.0 1.4775970430327976E-5 0.0 76-77 0.05088105417683438 7.018585954405788E-4 0.0 1.4775970430327976E-5 0.0 78-79 0.06313033366357627 7.092465806557427E-4 0.0 1.4775970430327976E-5 0.0 80-81 0.07798757193127105 7.092465806557427E-4 0.0 1.4775970430327976E-5 0.0 82-83 0.09798684790871996 7.314105363012348E-4 0.0 1.4775970430327976E-5 0.0 84-85 0.12361576862012383 7.757384475922187E-4 0.0 1.4775970430327976E-5 0.0 86-87 0.15432762315956056 7.831264328073827E-4 0.0 1.4775970430327976E-5 0.0 88-89 0.19256044664803418 8.052903884528746E-4 0.0 1.4775970430327976E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 2920 0.0 85.757835 1 TCGACGG 3445 0.0 73.49953 2 CGACGGC 4760 0.0 52.991375 3 GACGGCG 5450 0.0 46.28204 4 ACGGCGC 5730 0.0 44.019787 5 CGGCGCG 6475 0.0 39.025726 6 ATCTCGA 4355 0.0 37.751476 1 TCTCGAG 4985 0.0 33.449413 2 CTCGAGG 5645 0.0 29.452473 3 AGGGCGC 6110 0.0 27.51954 7 CGCGCCG 12210 0.0 26.647354 9 GGGCGCG 6395 0.0 26.070282 8 TCGAGGG 6545 0.0 25.32971 4 GGCGCGC 13010 0.0 25.154814 7 TATCTCG 3325 0.0 24.717299 2 GCGCGCC 13325 0.0 24.560158 8 ATATCTC 4130 0.0 20.249237 1 CGAGGGC 8565 0.0 20.021095 5 GAGGGCG 8695 0.0 19.829702 6 ATCCATA 8710 0.0 16.960012 16-17 >>END_MODULE