FastQCFastQC Report
Tue 31 May 2016
SRR1051291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17285507
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT8578774.962984308183729No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT738140.42702826130584426No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA246200.142431460066517No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA205600.11894357510022703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1197650.094.300261
TCGACGG1217150.092.781682
TATCTCG1140900.092.4095462
CGACGGC1226700.091.978063
ACGGCGC1234800.091.3746955
GACGGCG1237450.091.217414
ATATCTC1164950.090.648551
CGGCGCG1246350.090.607956
ATCTCGA1171850.089.948643
CTCGAGG1168350.089.417295
TCTCGAG1174650.088.9741064
AGGGCGC1176950.088.751819
TCGAGGG1181800.088.41176
CGAGGGC1183050.088.217917
GAGGGCG1205350.086.723728
CTCGACG17150.052.6163185
CGCGCCG2405900.047.189159
GCGCGCC2421500.046.9126058
GGCGCGC2420000.046.9102827
GGGCGCG1176350.044.47323210-11
Produced by FastQC (version 0.11.5)