##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051291_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17285507 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13517110027493 33.0 31.0 34.0 30.0 34.0 2 32.38430293077316 34.0 31.0 34.0 31.0 34.0 3 32.47207542133418 34.0 31.0 34.0 31.0 34.0 4 35.87656584212427 37.0 35.0 37.0 35.0 37.0 5 35.86815116270527 37.0 35.0 37.0 35.0 37.0 6 35.87567989761596 37.0 35.0 37.0 35.0 37.0 7 35.81098321269952 37.0 35.0 37.0 35.0 37.0 8 35.79006378002103 37.0 35.0 37.0 35.0 37.0 9 37.52485680634071 39.0 37.0 39.0 35.0 39.0 10-11 37.48191273764779 39.0 37.0 39.0 34.5 39.0 12-13 37.442983564207864 39.0 37.0 39.0 34.0 39.0 14-15 38.82479024190613 40.0 38.0 41.0 35.0 41.0 16-17 38.71655196460249 40.0 38.0 41.0 34.5 41.0 18-19 38.768827145191636 40.0 38.5 41.0 34.5 41.0 20-21 38.778391342527584 40.0 38.5 41.0 34.0 41.0 22-23 38.745555655382276 40.0 38.5 41.0 34.5 41.0 24-25 38.73032191071977 40.0 38.0 41.0 34.0 41.0 26-27 38.647491161236985 40.0 38.0 41.0 34.0 41.0 28-29 38.432234674979455 40.0 38.0 41.0 34.0 41.0 30-31 37.900081814204235 39.5 37.0 41.0 33.5 41.0 32-33 37.47762952512761 39.0 35.5 41.0 33.0 41.0 34-35 37.25282504007548 39.0 35.0 41.0 33.0 41.0 36-37 37.41368908068476 39.0 35.0 41.0 33.0 41.0 38-39 37.246523084338804 39.0 35.0 41.0 32.0 41.0 40-41 36.478450299432936 40.0 35.0 41.0 29.0 41.0 42-43 36.522282250673925 39.5 35.0 41.0 29.0 41.0 44-45 36.47699271997055 39.0 35.0 41.0 29.0 41.0 46-47 36.51430678313341 39.0 35.0 41.0 29.0 41.0 48-49 36.3592202704844 39.0 35.0 41.0 27.5 41.0 50-51 36.110819196683096 39.0 35.0 40.5 27.0 41.0 52-53 35.82744703409625 39.0 35.0 40.5 27.0 41.0 54-55 35.4681683331591 39.0 35.0 41.0 25.5 41.0 56-57 35.0053939985677 39.0 34.5 41.0 24.0 41.0 58-59 34.62136117847165 39.0 34.0 41.0 22.5 41.0 60-61 34.426171184912306 39.0 34.0 41.0 21.5 41.0 62-63 34.12792916632414 38.0 34.0 40.5 19.5 41.0 64-65 33.80540776154266 38.0 34.0 40.0 18.5 41.0 66-67 33.37439549791626 37.0 34.0 40.0 17.0 41.0 68-69 32.949251850119296 36.5 33.0 39.5 12.0 41.0 70-71 32.49615142905557 36.0 33.0 39.0 9.0 41.0 72-73 32.10403059048254 36.0 32.0 39.0 7.0 40.5 74-75 31.699007353385703 35.0 32.0 38.5 6.0 40.0 76-77 31.231687013866587 35.0 32.0 37.0 2.0 39.0 78-79 30.886267003912586 35.0 31.5 37.0 2.0 39.0 80-81 30.56261152768039 35.0 31.0 36.0 2.0 38.5 82-83 30.23557744068485 35.0 31.0 36.0 2.0 37.0 84-85 29.897509196577225 35.0 31.0 35.5 2.0 37.0 86-87 29.605687035966028 35.0 31.0 35.0 2.0 36.5 88-89 29.316261680956192 35.0 31.0 35.0 2.0 36.0 90-91 29.105438880097644 35.0 30.0 35.0 2.0 36.0 92-93 28.91021507208322 34.5 30.0 35.0 2.0 36.0 94-95 28.636173500725203 34.0 30.0 35.0 2.0 35.0 96-97 28.349782161437325 34.0 30.0 35.0 2.0 35.0 98-99 28.045612691603434 34.0 29.0 35.0 2.0 35.0 100-101 26.91988707649709 33.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36608669394259863 1101 2 0.37349646807517445 1101 3 0.3795414647781712 1101 4 0.3629898300423591 1101 5 0.3592439554957565 1101 6 0.3465487707507009 1101 7 0.3663671963864914 1101 8 0.3011540554046874 1101 9 0.4010540877329234 1101 10-11 0.3651111770219586 1101 12-13 0.38981076399891634 1101 14-15 0.5225906143405368 1101 16-17 0.45169608479700685 1101 18-19 0.5023002759531181 1101 20-21 0.4640999979977849 1101 22-23 0.44507661211598304 1101 24-25 0.468588289206032 1101 26-27 0.5273123126453214 1101 28-29 0.6014417731747912 1101 30-31 0.6815102175947132 1101 32-33 0.672303003481133 1101 34-35 0.7115426477568789 1101 36-37 0.7083838816834955 1101 38-39 0.7274934903704562 1101 40-41 0.7929445849949843 1101 42-43 0.7395638019253923 1101 44-45 0.780899243948447 1101 46-47 0.7456051380493633 1101 48-49 0.7124711746672432 1101 50-51 0.6404961142915369 1101 52-53 0.5572629612257671 1101 54-55 0.6190525232608053 1101 56-57 0.5719346076927891 1101 58-59 0.5169369153092944 1101 60-61 0.5256863292183382 1101 62-63 0.5246310808196881 1101 64-65 0.5258016184850618 1101 66-67 0.4854601858144463 1101 68-69 0.5225819709564021 1101 70-71 0.6160474156834255 1101 72-73 0.6440642061152246 1101 74-75 0.5707562628924983 1101 76-77 0.4736376776789406 1101 78-79 0.4070793781099411 1101 80-81 0.3940955375634587 1101 82-83 0.44134640741180675 1101 84-85 0.4749801562169509 1101 86-87 0.47574731568974826 1101 88-89 0.5236664783059872 1101 90-91 0.5591206793144465 1101 92-93 0.5318473800004391 1101 94-95 0.6002585788498997 1101 96-97 0.6075859887445922 1101 98-99 0.6882385374830413 1101 100-101 0.8573316105956295 1102 1 0.34449013318251076 1102 2 0.3274113545976718 1102 3 0.35396598413286284 1102 4 0.27608986490807297 1102 5 0.26775539895017886 1102 6 0.2809829900776606 1102 7 0.317771753744033 1102 8 0.28158813799756643 1102 9 0.34707301082594455 1102 10-11 0.3616708929547059 1102 12-13 0.35373237262574975 1102 14-15 0.5050451747998679 1102 16-17 0.44545718155741554 1102 18-19 0.5026227433445456 1102 20-21 0.4209401949792664 1102 22-23 0.4255890186474858 1102 24-25 0.4774558694683506 1102 26-27 0.5261850601894551 1102 28-29 0.5362783543595881 1102 30-31 0.573061979294998 1102 32-33 0.5673854895638044 1102 34-35 0.6207336266845189 1102 36-37 0.6462766485981248 1102 38-39 0.6431981106080045 1102 40-41 0.507137336655866 1102 42-43 0.5322351278572199 1102 44-45 0.5590588931043214 1102 46-47 0.5602676750622777 1102 48-49 0.5623658830486988 1102 50-51 0.4697700259206883 1102 52-53 0.46026124478613184 1102 54-55 0.5157833150679068 1102 56-57 0.5075142448380561 1102 58-59 0.4876640441548048 1102 60-61 0.43147531796333993 1102 62-63 0.5310101291250007 1102 64-65 0.45807023336200103 1102 66-67 0.4788664598570094 1102 68-69 0.4911241520936116 1102 70-71 0.5836557646778715 1102 72-73 0.5534560346885797 1102 74-75 0.5534114460071997 1102 76-77 0.4452703323577687 1102 78-79 0.3754123907120501 1102 80-81 0.4417050507116258 1102 82-83 0.4957804711583478 1102 84-85 0.427649178626222 1102 86-87 0.4148853025558168 1102 88-89 0.5097804482684616 1102 90-91 0.5495211778883089 1102 92-93 0.47783725446422665 1102 94-95 0.5489349208511705 1102 96-97 0.5621187613896872 1102 98-99 0.6507066287754704 1102 100-101 0.7170369643678107 1103 1 0.2576867189169718 1103 2 0.2288278171161977 1103 3 0.28133166803895904 1103 4 0.24269901648222714 1103 5 0.23588736963093027 1103 6 0.2510990359718619 1103 7 0.26998179962550495 1103 8 0.2581921810571117 1103 9 0.3027761189526643 1103 10-11 0.29499206190654803 1103 12-13 0.3290121879837571 1103 14-15 0.41549065404478114 1103 16-17 0.4623152333515037 1103 18-19 0.43362173367952295 1103 20-21 0.4078117888770123 1103 22-23 0.406687779596254 1103 24-25 0.416802707181553 1103 26-27 0.43288505964562063 1103 28-29 0.4362877401588179 1103 30-31 0.464670820989447 1103 32-33 0.4246191780468678 1103 34-35 0.44734314623222105 1103 36-37 0.37291868412862783 1103 38-39 0.38140548012170683 1103 40-41 0.37095377824342535 1103 42-43 0.38280403873165625 1103 44-45 0.3363547517864518 1103 46-47 0.3464351143193909 1103 48-49 0.35280198776273863 1103 50-51 0.3258850057393943 1103 52-53 0.3941728917414551 1103 54-55 0.34459108454149145 1103 56-57 0.36610534254254645 1103 58-59 0.34903155748141756 1103 60-61 0.28141949615840645 1103 62-63 0.3784895806052262 1103 64-65 0.3882647251479412 1103 66-67 0.3611392286536841 1103 68-69 0.3493193286655867 1103 70-71 0.3909106464246008 1103 72-73 0.40866548169145744 1103 74-75 0.36220485126833424 1103 76-77 0.39095006849513325 1103 78-79 0.31841389533946796 1103 80-81 0.3561282769489651 1103 82-83 0.42186914708598167 1103 84-85 0.3276836811715107 1103 86-87 0.37562513290437494 1103 88-89 0.4507285438170463 1103 90-91 0.4494854005247291 1103 92-93 0.37808815017888975 1103 94-95 0.4291132412625842 1103 96-97 0.4463565329397703 1103 98-99 0.5367886625072877 1103 100-101 0.670376800033111 1104 1 0.26313144579188474 1104 2 0.22190441650188575 1104 3 0.18007136708703797 1104 4 0.18806475917399013 1104 5 0.18803361057717893 1104 6 0.1851084621261947 1104 7 0.21981359291459768 1104 8 0.19545989512990047 1104 9 0.20420806387975432 1104 10-11 0.20941665307992707 1104 12-13 0.272515400276653 1104 14-15 0.31707411178241074 1104 16-17 0.3613883643231546 1104 18-19 0.3342260517659241 1104 20-21 0.3709894557275959 1104 22-23 0.37444425072343535 1104 24-25 0.35294342564061054 1104 26-27 0.3494883939690041 1104 28-29 0.3518162516296073 1104 30-31 0.39598934297356436 1104 32-33 0.34763882832646686 1104 34-35 0.4158096873416852 1104 36-37 0.36097018124947766 1104 38-39 0.3241895313178773 1104 40-41 0.23974912722236752 1104 42-43 0.19844694920398354 1104 44-45 0.22434985840852306 1104 46-47 0.19796233751460335 1104 48-49 0.17045729888861416 1104 50-51 0.09817688343414233 1104 52-53 0.1455945549604607 1104 54-55 0.1760564814500043 1104 56-57 0.13954743467485997 1104 58-59 0.16139166099880953 1104 60-61 0.06365122629313191 1104 62-63 0.17323774546228066 1104 64-65 0.12359346634693935 1104 66-67 0.19765133694613013 1104 68-69 0.2459293727777876 1104 70-71 0.23244024243871308 1104 72-73 0.21195275328611274 1104 74-75 0.2159283393620548 1104 76-77 0.2525613207676862 1104 78-79 0.17695027958395215 1104 80-81 0.1650899808040407 1104 82-83 0.17678757658624988 1104 84-85 0.16817977758045188 1104 86-87 0.2338201317969144 1104 88-89 0.26612480637241376 1104 90-91 0.31443748865723364 1104 92-93 0.2084771249040962 1104 94-95 0.28167816900596776 1104 96-97 0.2866542915931092 1104 98-99 0.34166909225578124 1104 100-101 0.3770915213601249 1105 1 0.06564616069643137 1105 2 0.07989967198913206 1105 3 0.08392025438733697 1105 4 -0.04304627650711268 1105 5 0.09319998585595357 1105 6 0.06349847585200052 1105 7 0.1078735557756687 1105 8 0.11055220409978972 1105 9 0.12594316433832375 1105 10-11 0.05297889299768599 1105 12-13 0.11610482815333256 1105 14-15 0.21915975866827608 1105 16-17 0.0939265918153751 1105 18-19 0.15563354529218287 1105 20-21 0.09909927765207271 1105 22-23 0.0978178294772647 1105 24-25 0.10487270663505655 1105 26-27 0.1540062983001107 1105 28-29 0.20271179025304775 1105 30-31 0.20876929559158697 1105 32-33 0.12544455748506778 1105 34-35 0.23298038560223233 1105 36-37 0.2023583522376029 1105 38-39 0.22293382999294664 1105 40-41 0.09907967918797311 1105 42-43 0.04718250308464178 1105 44-45 0.008598125000496282 1105 46-47 0.1170161804404728 1105 48-49 0.10627180725877139 1105 50-51 0.10427720401871454 1105 52-53 -0.0136358841864066 1105 54-55 0.10968807821251403 1105 56-57 0.1589273136591558 1105 58-59 0.16120680308632274 1105 60-61 0.048175373071799754 1105 62-63 0.16693715029843048 1105 64-65 0.10961723722100913 1105 66-67 0.10611572010420645 1105 68-69 0.16366395319027305 1105 70-71 0.17425823609649171 1105 72-73 0.19020232772927415 1105 74-75 0.18183172919738055 1105 76-77 0.3161971217886439 1105 78-79 0.25146685617139397 1105 80-81 0.2236830682643003 1105 82-83 0.2584297555130455 1105 84-85 0.2272831638161854 1105 86-87 0.2552358964872674 1105 88-89 0.27009684860634664 1105 90-91 0.392411820735866 1105 92-93 0.3220340775287518 1105 94-95 0.3822847915576517 1105 96-97 0.3007771645588271 1105 98-99 0.32545038935239745 1105 100-101 0.3862304661812459 1106 1 -0.10903809847399515 1106 2 -0.14944121171147629 1106 3 -0.16074965887382575 1106 4 -0.3147987133691217 1106 5 -0.12939738488880437 1106 6 -0.07638087631972468 1106 7 -0.1002834811861959 1106 8 -0.09660295029184596 1106 9 -0.11764893452264857 1106 10-11 -0.11284123551818936 1106 12-13 -0.13548052168149383 1106 14-15 -0.25763474199602143 1106 16-17 -0.21403238676011682 1106 18-19 -0.27835874972821273 1106 20-21 -0.342721311529246 1106 22-23 -0.18412064433259445 1106 24-25 -0.07856053929607043 1106 26-27 -0.4005453695704233 1106 28-29 -0.2691804346499964 1106 30-31 -0.22097721155744665 1106 32-33 -0.26944804773786757 1106 34-35 -0.1842146429111864 1106 36-37 -0.36269703068955295 1106 38-39 -0.19630815203660745 1106 40-41 -0.1843116826611535 1106 42-43 -0.20362085726224421 1106 44-45 -0.2409521011431437 1106 46-47 -0.1249260027436776 1106 48-49 -0.0745300373587483 1106 50-51 -0.2309238210741995 1106 52-53 -0.2095484365134226 1106 54-55 -0.1670202029556762 1106 56-57 -0.06454140324620283 1106 58-59 -0.00848519066469322 1106 60-61 -0.059957679157825794 1106 62-63 0.11889486739593735 1106 64-65 0.04299459410087536 1106 66-67 0.014605825047659948 1106 68-69 0.11537360340434333 1106 70-71 0.13962762211640722 1106 72-73 0.0945325722519641 1106 74-75 0.15433961905103288 1106 76-77 0.21922425929080447 1106 78-79 0.041573217556688746 1106 80-81 0.0019064933792094507 1106 82-83 0.09348281793387159 1106 84-85 0.034129207207538315 1106 86-87 0.08148821534174999 1106 88-89 0.1535673798939321 1106 90-91 0.17287291565012808 1106 92-93 0.07983854156975667 1106 94-95 0.1069394074491008 1106 96-97 -0.05588110811229541 1106 98-99 0.03929730253718233 1106 100-101 0.07900552216544199 1107 1 -0.24346452857339784 1107 2 -0.31274061405128606 1107 3 -0.3177278005831141 1107 4 -0.2089813611416531 1107 5 -0.26048633786332687 1107 6 -0.1738762198211674 1107 7 -0.21792580979840181 1107 8 -0.21267454117827356 1107 9 -0.18039138687723266 1107 10-11 -0.27421395537732707 1107 12-13 -0.16268265709557994 1107 14-15 -0.2395277349780187 1107 16-17 -0.2725630460679156 1107 18-19 -0.26967022621263226 1107 20-21 -0.26849166042119066 1107 22-23 -0.24071719517775136 1107 24-25 -0.33805025679041734 1107 26-27 -0.2704456155042081 1107 28-29 -0.3071250282879774 1107 30-31 -0.18466242554196555 1107 32-33 -0.27661744218768547 1107 34-35 -0.3712619250045819 1107 36-37 -0.2860212643411586 1107 38-39 -0.26649134168153665 1107 40-41 -0.24132684991416653 1107 42-43 -0.2218219849699068 1107 44-45 -0.20722784222436275 1107 46-47 -0.17644517988958341 1107 48-49 -0.16558561573221908 1107 50-51 -0.1487288615402207 1107 52-53 -0.10260809279100869 1107 54-55 -0.11402004296244428 1107 56-57 -0.12221905051432458 1107 58-59 -0.15739183976069882 1107 60-61 -0.18243676372794226 1107 62-63 -0.17525693510743423 1107 64-65 -0.24324255767081127 1107 66-67 -0.15434484074958021 1107 68-69 -0.08769908485464839 1107 70-71 0.0025597112079509543 1107 72-73 0.01582786679407633 1107 74-75 -0.04125065536412009 1107 76-77 -0.3047816248611035 1107 78-79 -0.3301184764745102 1107 80-81 -0.19649977242136885 1107 82-83 -0.1318961956884266 1107 84-85 -0.21250095031199834 1107 86-87 -0.23288119204193336 1107 88-89 -0.1514967073942124 1107 90-91 -0.05423393044100422 1107 92-93 -0.1852849539510366 1107 94-95 -0.12499356590870647 1107 96-97 -0.1502044148758941 1107 98-99 -0.20393846974392105 1107 100-101 -0.2839113213857196 1108 1 -0.4815612672561258 1108 2 -0.41929647598083974 1108 3 -0.39271125478047253 1108 4 -0.4827613444620553 1108 5 -0.7103872690570157 1108 6 -0.46843464836139503 1108 7 -0.4822561213016954 1108 8 -1.210767516689856 1108 9 -0.7608263275900597 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2305 22-23 0.1963848676328297 2305 24-25 0.17677472796111005 2305 26-27 0.13874310513806876 2305 28-29 0.13634972215022856 2305 30-31 0.056082737926338666 2305 32-33 0.15586450956127607 2305 34-35 0.13547115300035983 2305 36-37 0.03612150106909695 2305 38-39 0.13509713519565736 2305 40-41 0.027365012160039726 2305 42-43 0.04766931818885922 2305 44-45 0.07614015049333744 2305 46-47 0.05648965512627768 2305 48-49 0.07978633055629558 2305 50-51 0.10451664450007314 2305 52-53 0.0907690200874427 2305 54-55 0.07408796713745858 2305 56-57 0.1787403339028515 2305 58-59 0.1069724982168978 2305 60-61 0.23128756531475148 2305 62-63 0.23237549588873918 2305 64-65 0.12645819213876308 2305 66-67 0.159622335595202 2305 68-69 0.09810723044279968 2305 70-71 0.17291672390149415 2305 72-73 0.09640393392840707 2305 74-75 0.1619106170657112 2305 76-77 0.22999384478191232 2305 78-79 0.15183524730072406 2305 80-81 0.15863232370151792 2305 82-83 0.09308293565097614 2305 84-85 0.10754731527855199 2305 86-87 0.12740486956093022 2305 88-89 0.12372057571284145 2305 90-91 0.17597761236948273 2305 92-93 0.21901751780645284 2305 94-95 0.28459901955237044 2305 96-97 0.34125190361584146 2305 98-99 0.28814582482894124 2305 100-101 0.3827600012900021 2306 1 -0.24813561820396401 2306 2 -0.47300483607874 2306 3 -0.3169907693139038 2306 4 -0.22329033993572978 2306 5 -0.18795341707117075 2306 6 -0.21711097268524782 2306 7 -0.09160576700384127 2306 8 -0.11144753260016671 2306 9 -0.10548831971537709 2306 10-11 -0.07459544390917472 2306 12-13 -0.09579854684855604 2306 14-15 -0.1966569660140678 2306 16-17 -0.13538917180096632 2306 18-19 -0.11051426424679534 2306 20-21 -0.07982875197877348 2306 22-23 -0.14081318929718378 2306 24-25 -0.1828190483271399 2306 26-27 -0.0864776677492003 2306 28-29 -0.07734212578245803 2306 30-31 -0.10061806054306288 2306 32-33 -0.07133823228451064 2306 34-35 -0.0928386061156985 2306 36-37 -0.2831292651187667 2306 38-39 -0.23197638003610166 2306 40-41 -0.13544954895851902 2306 42-43 -0.10195237799138823 2306 44-45 -0.01057768366183609 2306 46-47 -0.1788936132992518 2306 48-49 -0.16361431443102958 2306 50-51 -0.36684981503487535 2306 52-53 -0.12386244768504895 2306 54-55 -0.19247764803231604 2306 56-57 -0.10259897578641386 2306 58-59 -0.06441915661556408 2306 60-61 -0.02881308336229438 2306 62-63 -0.05242998323925008 2306 64-65 -0.054309259041595226 2306 66-67 -0.04670300727104859 2306 68-69 -0.06237853320455855 2306 70-71 -0.37857988220072514 2306 72-73 -0.29728491685040126 2306 74-75 -0.1666866141198753 2306 76-77 -0.10496064039455888 2306 78-79 -0.16071315451769763 2306 80-81 -0.1197772659807832 2306 82-83 -2.2024477541648935E-4 2306 84-85 -0.06178788368215393 2306 86-87 -0.12748947024040902 2306 88-89 -0.2595138496329703 2306 90-91 -0.21586725423019715 2306 92-93 -0.08851841325974519 2306 94-95 0.022247658814464444 2306 96-97 0.03635542858440388 2306 98-99 -0.014894653256263268 2306 100-101 0.06906503117906482 2307 1 -0.3819941104794182 2307 2 -0.3197037671160956 2307 3 -0.5197656579374268 2307 4 -0.2801640577479958 2307 5 -0.3710242256164591 2307 6 -0.28146894972504555 2307 7 -0.22529067391583624 2307 8 -0.2455275595800117 2307 9 -0.2851923796784348 2307 10-11 -0.4359015797041934 2307 12-13 -0.2752217456322086 2307 14-15 -0.4425943535414447 2307 16-17 -0.5691458009593617 2307 18-19 -0.5608642252385891 2307 20-21 -0.6740454598468943 2307 22-23 -0.6233203432822307 2307 24-25 -0.4635464352500236 2307 26-27 -0.3453270629680034 2307 28-29 -0.34808835532857785 2307 30-31 -0.2698314574311027 2307 32-33 -0.32249236030789064 2307 34-35 -0.2573368836302734 2307 36-37 -0.39164747309907 2307 38-39 -0.36115536027075024 2307 40-41 -0.33423390499297767 2307 42-43 -0.482647656477063 2307 44-45 -0.5200905193187495 2307 46-47 -0.3261418915706997 2307 48-49 -0.385737764813193 2307 50-51 -0.24334085867507582 2307 52-53 -0.2660361214448912 2307 54-55 -0.4021286399764392 2307 56-57 -0.2881131002781032 2307 58-59 -0.2570817630957336 2307 60-61 -0.36673099697297573 2307 62-63 -0.21327485561438664 2307 64-65 -0.3361797152418191 2307 66-67 -0.42215819101253516 2307 68-69 -0.43607022334562373 2307 70-71 -0.39868972662622326 2307 72-73 -0.4278325108140635 2307 74-75 -0.442779037774649 2307 76-77 -0.7272623694762892 2307 78-79 -0.6652152237425177 2307 80-81 -0.5554725031833385 2307 82-83 -0.4205667059927336 2307 84-85 -0.3933087396962911 2307 86-87 -0.4746946321276013 2307 88-89 -0.47061603688439035 2307 90-91 -0.45176528120742176 2307 92-93 -0.4222341178518896 2307 94-95 -0.2961321422770915 2307 96-97 -0.35490940677272675 2307 98-99 -0.4137600989936203 2307 100-101 -0.3396554912936054 2308 1 -0.5712349691836529 2308 2 -0.49209165534709953 2308 3 -0.4563126043296748 2308 4 -0.6901524196082036 2308 5 -0.4591977553273807 2308 6 -0.7401048529756054 2308 7 -1.4094123529147637 2308 8 -0.5661075391243884 2308 9 -0.6339055331136763 2308 10-11 -0.4351776003899346 2308 12-13 -0.37028032271097544 2308 14-15 -0.948432687247518 2308 16-17 -1.2099895704692614 2308 18-19 -0.7143644658598376 2308 20-21 -0.5865331143557597 2308 22-23 -0.5865611554222312 2308 24-25 -0.5993223594137334 2308 26-27 -0.5610133837897848 2308 28-29 -0.9132524284419574 2308 30-31 -1.396725626011083 2308 32-33 -0.6897280889934478 2308 34-35 -0.9780083908086326 2308 36-37 -1.2004336592944966 2308 38-39 -0.7959620859801007 2308 40-41 -0.8456304046733223 2308 42-43 -0.6544055491389642 2308 44-45 -0.826046779863141 2308 46-47 -1.0578130895585005 2308 48-49 -1.76055405703908 2308 50-51 -2.6033269149872993 2308 52-53 -1.7930195316210202 2308 54-55 -1.3311370337003865 2308 56-57 -0.656560169866502 2308 58-59 -0.7461257671405122 2308 60-61 -0.19610609126711154 2308 62-63 -0.19524981186494728 2308 64-65 -0.24328676092630275 2308 66-67 -0.3183174300782028 2308 68-69 -0.7133149384853255 2308 70-71 -0.9422933373851485 2308 72-73 -1.0361115609965594 2308 74-75 -1.0512227861442227 2308 76-77 -0.7998846104818611 2308 78-79 -0.48488146213970396 2308 80-81 -0.6292905301808602 2308 82-83 -0.5734359755373504 2308 84-85 -0.8543839477949078 2308 86-87 -0.6978387887748596 2308 88-89 -0.7585886408177807 2308 90-91 -1.1252050394950253 2308 92-93 -0.7767396556327171 2308 94-95 -1.5701977463839114 2308 96-97 -1.0109776788354772 2308 98-99 -1.4996729205623858 2308 100-101 -1.7531890475266607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 336.0 11 2237.0 12 5970.0 13 11078.0 14 18058.0 15 26780.0 16 39758.0 17 67079.0 18 142501.0 19 353986.0 20 414963.0 21 306720.0 22 257573.0 23 212251.0 24 199909.0 25 205956.0 26 225032.0 27 246250.0 28 262717.0 29 281700.0 30 308833.0 31 352465.0 32 419552.0 33 528537.0 34 723172.0 35 1061799.0 36 1793201.0 37 3430051.0 38 4378717.0 39 1007521.0 40 805.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 24.759626662961058 32.82515809342474 23.30361498797808 19.111600255636123 2 19.767606469396586 26.063794368310976 36.957620045509806 17.21097911678263 3 19.271711266554114 30.989822861429523 24.093183960412617 25.64528191160375 4 26.54636627088809 24.000308466508965 30.31864208553443 19.134683177068514 5 19.88540457621521 31.12532365987298 23.713507506606547 25.275764257305266 6 19.715071128662874 23.969832067986204 29.521830051036396 26.793266752314526 7 26.635868997073676 23.867468857002574 23.683164167530638 25.813497978393112 8 32.48783113436193 23.960039121793717 23.839510396745112 19.71261934709924 9 19.13041370438252 30.03596018329112 24.18315528725886 26.650470825067497 10-11 29.08074955510417 23.820267464529678 24.097340621828447 23.001642358537705 12-13 25.688393995095716 23.77821544057334 24.296213049494735 26.2371775148362 14-15 25.683420798707264 23.726853947645274 24.29649590260789 26.29322935103957 16-17 25.697831715320817 27.147829681825357 24.271041051905506 22.883297550948317 18-19 22.263162428501516 23.661432088743478 27.739825623859343 26.335579858895663 20-21 22.250773436960802 30.32402231534198 24.317481691454002 23.107722556243214 22-23 18.983190309846872 27.01532717642168 31.098262324516273 22.903220189215173 24-25 19.003012176617094 23.635893352737643 34.47541342004027 22.885681050604994 26-27 19.015180353475454 23.64319805617425 37.78289317636169 19.558728413988604 28-29 18.99845344426403 23.579163746831377 37.8836675140625 19.538715294842092 30-31 19.01878961690393 23.541445491013494 37.83761682131473 19.60214807076784 32-33 19.086263253648386 23.553703014367912 37.79536169024639 19.564672041737314 34-35 19.101243916343453 23.597242989772894 37.7794681082352 19.522044985648453 36-37 19.094131869360133 23.574245432421428 37.73079429000466 19.60082840821378 38-39 19.33294040990832 23.65503541011443 36.9970950182854 20.014929161691846 40-41 20.187836111221195 24.43316942449862 34.187725610883184 21.191268853397002 42-43 20.310696423926636 24.715503591994587 34.3754628262578 20.598337157820975 44-45 20.410804734393665 25.21931161206796 33.87490169946919 20.494981954069193 46-47 20.15208963795584 25.645337153310965 33.836003227488476 20.366569981244723 48-49 20.217601628376205 25.84500233014021 33.38760733346657 20.549788708017015 50-51 20.321554034065265 26.170769827862284 32.842133623572984 20.66554251449947 52-53 20.423261797038094 26.40850405535614 32.37995413986503 20.78828000774074 54-55 20.735599500735447 26.49090780238264 31.663583626089608 21.109909070792302 56-57 20.93975027750126 26.59301517739688 31.077604492596024 21.389630052505836 58-59 21.100895351906054 26.731680332653497 30.604635887178 21.56278842826245 60-61 21.22183083568298 26.83098537567443 30.099990382063492 21.847193406579102 62-63 21.405067840937498 27.00890115632709 29.618732039505698 21.967298963229716 64-65 21.53467063476935 27.13416794774952 29.303768758417092 22.027392659064034 66-67 21.60015002171184 27.180750013681987 28.967710389368012 22.251389575238157 68-69 21.65130847190904 27.296513200429867 28.69024948689503 22.361928840766062 70-71 21.70487089854111 27.423118808263787 28.543436407416177 22.32857388577893 72-73 21.72640468043948 27.46763697759858 28.3920083857469 22.41394995621504 74-75 21.82323583201453 27.508520943251575 28.284654509223262 22.383588715510637 76-77 21.820566110211296 27.554892609201083 28.210523726816266 22.414017553771355 78-79 21.873673607728225 27.49273905827042 28.130767658287 22.502819675714356 80-81 21.887162441182053 27.52614924401949 28.07880518843231 22.507883126366146 82-83 21.907542071212905 27.616172051217085 28.0315581427282 22.444727734841805 84-85 21.941463101101487 27.567144477717058 27.942700162412386 22.54869225876907 86-87 21.973233510165397 27.636608301334682 27.84212766591366 22.54803052258626 88-89 21.990439497766427 27.680402613477018 27.778149972351535 22.551007916405023 90-91 22.025686664142587 27.596356519839322 27.71858319569635 22.65937362032174 92-93 21.995571491708557 27.54005820310307 27.77641520384898 22.68795510133939 94-95 22.02695989136555 27.598753694752986 27.70930883224762 22.664977581633845 96-97 22.053488493858502 27.565695553781964 27.69290226801929 22.687913684340245 98-99 22.12808165337379 27.612363422308455 27.64223569066423 22.617319233653525 100-101 22.25578277936141 27.826034336403172 27.243774740023476 22.674408144211945 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 254.0 1 180.5 2 93.0 3 70.5 4 65.0 5 84.5 6 95.0 7 100.0 8 122.0 9 134.0 10 149.0 11 162.5 12 197.5 13 311.0 14 474.5 15 923.0 16 12506.5 17 21399.0 18 20889.5 19 23515.0 20 26653.0 21 32385.0 22 40358.5 23 50628.0 24 61877.0 25 76104.0 26 95027.0 27 118060.5 28 143044.5 29 175624.5 30 220712.5 31 269841.0 32 321707.0 33 384643.0 34 443228.0 35 501564.5 36 566282.0 37 617552.5 38 657500.5 39 694577.5 40 723601.0 41 723885.0 42 709538.5 43 706279.0 44 699929.0 45 693641.5 46 674685.5 47 648381.5 48 624932.5 49 592702.0 50 557628.5 51 523650.5 52 499618.5 53 472813.5 54 435409.0 55 391655.5 56 346204.5 57 304432.5 58 269034.5 59 231743.0 60 187443.0 61 149685.0 62 121346.0 63 93918.5 64 71979.5 65 56606.5 66 42477.0 67 30541.5 68 23323.5 69 18767.0 70 15341.5 71 13602.5 72 12605.5 73 10484.5 74 8031.5 75 6436.0 76 4987.0 77 3422.5 78 2194.0 79 1429.5 80 820.0 81 505.5 82 337.0 83 228.0 84 143.5 85 67.5 86 23.5 87 6.5 88 5.5 89 4.0 90 1.5 91 0.5 92 0.0 93 0.5 94 1.0 95 1.5 96 2.0 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 5.785193341450731E-6 9 0.0 10-11 0.0 12-13 1.4462983353626828E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.785193341450731E-6 24-25 0.0 26-27 4.049635339015512E-5 28-29 0.0 30-31 0.002941770814127697 32-33 0.005906682401621196 34-35 0.0015099354621186409 36-37 0.0017876247425082758 38-39 0.0031500377744199228 40-41 0.0028463151239937596 42-43 0.0023314329166046442 44-45 0.0065922278125831076 46-47 0.0013884464019481755 48-49 3.847153572064736E-4 50-51 0.002779785400567076 52-53 0.00197853612277615 54-55 4.7438585399895995E-4 56-57 0.0 58-59 7.52075134388595E-5 60-61 5.46700770767094E-4 62-63 0.0 64-65 0.0 66-67 5.2066740073056575E-5 68-69 0.0059703195283771545 70-71 0.006236438422083887 72-73 0.0026814371137624136 74-75 0.0034450826348339106 76-77 0.03323304314996372 78-79 0.05801102623139721 80-81 0.04959935511292784 82-83 8.243900511567291E-4 84-85 0.001084723751522012 86-87 0.0015880355722282255 88-89 0.0037083089318699185 90-91 2.8925966707253656E-6 92-93 1.4462983353626828E-5 94-95 8.38853034510356E-5 96-97 0.006028171461791661 98-99 0.0014144797719847038 100-101 2.3140773365802925E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7285507E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.066242740563034 #Duplication Level Percentage of deduplicated Percentage of total 1 77.41717585551538 35.663184152490274 2 12.615842600768906 11.62328935247513 3 4.165732690588309 5.756989599508195 4 1.8845181808242006 3.4725068786740754 5 1.0358339098626748 2.385848816532023 6 0.6031479416809283 1.6670855693966762 7 0.39340866698605115 1.2686001404734533 8 0.28022006560692403 1.0326948450420048 9 0.21054728257694563 0.8729210004799948 >10 1.0554855607236762 9.350458658715489 >50 0.1799027252826447 5.880192380431993 >100 0.1552669369364143 13.557091281530322 >500 0.002067069019673307 0.5855256142403867 >1k 6.601154695419213E-4 0.595546491856241 >5k 7.615926309307005E-5 0.2272781271812433 >10k+ 1.1423889463960507E-4 6.06078709097255 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 857877 4.962984308183729 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 73814 0.42702826130584426 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 24620 0.142431460066517 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 20560 0.11894357510022703 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.942232009740877E-5 5.785193341450731E-6 0.0 1.1570386682901462E-5 0.0 2 6.942232009740877E-5 5.785193341450731E-6 0.0 1.7355580024352193E-5 0.0 3 7.52075134388595E-5 5.785193341450731E-6 0.0 2.3140773365802925E-5 0.0 4 7.52075134388595E-5 5.785193341450731E-6 0.0 2.3140773365802925E-5 0.0 5 8.099270678031024E-5 5.785193341450731E-6 0.0 2.3140773365802925E-5 0.0 6 8.099270678031024E-5 5.785193341450731E-6 0.0 2.3140773365802925E-5 0.0 7 8.677790012176097E-5 5.785193341450731E-6 0.0 2.3140773365802925E-5 0.0 8 9.834828680466243E-5 5.785193341450731E-6 0.0 2.8925966707253653E-5 0.0 9 9.834828680466243E-5 5.785193341450731E-6 0.0 3.4711160048704386E-5 0.0 10-11 9.834828680466243E-5 5.785193341450731E-6 0.0 4.049635339015512E-5 2.8925966707253656E-6 12-13 1.0413348014611316E-4 5.785193341450731E-6 0.0 4.628154673160585E-5 5.785193341450731E-6 14-15 1.0991867348756388E-4 5.785193341450731E-6 0.0 5.206674007305658E-5 1.1570386682901462E-5 16-17 1.2727425351191608E-4 5.785193341450731E-6 0.0 5.206674007305658E-5 1.7355580024352193E-5 18-19 1.3305944685336682E-4 5.785193341450731E-6 0.0 5.785193341450731E-5 1.7355580024352193E-5 20-21 1.3595204352409218E-4 1.1570386682901462E-5 0.0 6.074453008523267E-5 2.024817669507756E-5 22-23 1.50415026877719E-4 1.4462983353626827E-5 0.0 6.363712675595804E-5 2.3140773365802925E-5 24-25 1.6487801023134583E-4 1.7355580024352193E-5 0.0 6.65297234266834E-5 2.6033370036528287E-5 26-27 1.8512618692642337E-4 1.7355580024352193E-5 0.0 6.942232009740877E-5 3.4711160048704386E-5 28-29 2.1405215363367705E-4 1.7355580024352193E-5 0.0 7.231491676813414E-5 3.4711160048704386E-5 30-31 2.256225403165785E-4 1.7355580024352193E-5 0.0 8.388530345103561E-5 3.760375671942975E-5 32-33 2.3140773365802923E-4 1.7355580024352193E-5 0.0 9.545569013393707E-5 4.049635339015512E-5 34-35 2.4876331368238144E-4 2.3140773365802925E-5 0.0 1.0413348014611315E-4 4.3388950060880484E-5 36-37 2.603337003652829E-4 2.3140773365802925E-5 0.0 1.1570386682901461E-4 4.9174143402331216E-5 38-39 2.8058187706036044E-4 2.8925966707253653E-5 0.0 1.2148906017046535E-4 5.206674007305658E-5 40-41 3.210782304505156E-4 3.4711160048704386E-5 0.0 1.330594468533668E-4 5.206674007305658E-5 42-43 3.4711160048704387E-4 4.628154673160585E-5 0.0 1.417372368655429E-4 5.785193341450731E-5 44-45 3.64467180511396E-4 4.9174143402331216E-5 0.0 1.5620022021916972E-4 6.65297234266834E-5 46-47 3.760375671942975E-4 5.206674007305658E-5 0.0 1.677706069020712E-4 7.52075134388595E-5 48-49 4.19426517255178E-4 5.785193341450731E-5 0.0 1.764483969142473E-4 8.099270678031024E-5 50-51 4.6281546731605846E-4 5.785193341450731E-5 0.0 1.9091138026787414E-4 8.099270678031024E-5 52-53 6.479416542424819E-4 6.65297234266834E-5 0.0 2.0248176695077558E-4 8.099270678031024E-5 54-55 9.574494980100959E-4 6.942232009740877E-5 0.0 2.1405215363367705E-4 8.677790012176097E-5 56-57 0.0014752243020699364 6.942232009740877E-5 0.0 2.2851513698730387E-4 8.677790012176097E-5 58-59 0.0021000251829466155 7.231491676813414E-5 0.0 2.3719292699947997E-4 8.677790012176097E-5 60-61 0.0029475560074691473 8.388530345103561E-5 0.0 2.4008552367020533E-4 8.677790012176097E-5 62-63 0.004153768819161625 9.25630934632117E-5 0.0 2.603337003652829E-4 8.677790012176097E-5 64-65 0.005646348701255913 9.25630934632117E-5 0.0 2.921522637432619E-4 8.677790012176097E-5 66-67 0.007428188250422739 1.0124088347538779E-4 0.0 3.0661524709688873E-4 8.677790012176097E-5 68-69 0.010069129010794996 1.0991867348756388E-4 0.0 3.0661524709688873E-4 8.677790012176097E-5 70-71 0.013528674628982534 1.1281127015828925E-4 0.0 3.0661524709688873E-4 8.677790012176097E-5 72-73 0.01824360720226488 1.1570386682901461E-4 0.0 3.095078437676141E-4 8.677790012176097E-5 74-75 0.02428045645406872 1.1570386682901461E-4 0.0 3.1240044043833945E-4 8.677790012176097E-5 76-77 0.03206154149831995 1.1570386682901461E-4 0.0 3.1240044043833945E-4 9.25630934632117E-5 78-79 0.04205257039900536 1.1570386682901461E-4 0.0 3.1240044043833945E-4 1.0124088347538779E-4 80-81 0.054600654756611994 1.2148906017046535E-4 0.0 3.268634237919663E-4 1.0413348014611316E-4 82-83 0.06979546506793234 1.3595204352409218E-4 0.0 3.355412138041424E-4 1.0413348014611316E-4 84-85 0.08930892220864566 1.417372368655429E-4 0.0 3.3843381047486774E-4 1.0413348014611316E-4 86-87 0.11295589999182552 1.50415026877719E-4 0.0 3.4132640714559315E-4 1.0991867348756388E-4 88-89 0.14224633387959057 1.5330762354844437E-4 0.0 3.4711160048704387E-4 1.0991867348756388E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 119765 0.0 94.30026 1 TCGACGG 121715 0.0 92.78168 2 TATCTCG 114090 0.0 92.409546 2 CGACGGC 122670 0.0 91.97806 3 ACGGCGC 123480 0.0 91.374695 5 GACGGCG 123745 0.0 91.21741 4 ATATCTC 116495 0.0 90.64855 1 CGGCGCG 124635 0.0 90.60795 6 ATCTCGA 117185 0.0 89.94864 3 CTCGAGG 116835 0.0 89.41729 5 TCTCGAG 117465 0.0 88.974106 4 AGGGCGC 117695 0.0 88.75181 9 TCGAGGG 118180 0.0 88.4117 6 CGAGGGC 118305 0.0 88.21791 7 GAGGGCG 120535 0.0 86.72372 8 CTCGACG 1715 0.0 52.616318 5 CGCGCCG 240590 0.0 47.18915 9 GCGCGCC 242150 0.0 46.912605 8 GGCGCGC 242000 0.0 46.910282 7 GGGCGCG 117635 0.0 44.473232 10-11 >>END_MODULE