##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051291_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17285507 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29237389450017 33.0 31.0 34.0 31.0 34.0 2 32.564438173551984 34.0 31.0 34.0 31.0 34.0 3 32.69324139581211 34.0 31.0 34.0 31.0 34.0 4 36.20613540580557 37.0 35.0 37.0 35.0 37.0 5 36.114056533025035 37.0 35.0 37.0 35.0 37.0 6 36.12071095166604 37.0 36.0 37.0 35.0 37.0 7 36.090247049160894 37.0 35.0 37.0 35.0 37.0 8 36.09725731504433 37.0 35.0 37.0 35.0 37.0 9 37.88484260253402 39.0 38.0 39.0 35.0 39.0 10-11 37.85578837809039 39.0 38.0 39.0 35.0 39.0 12-13 37.8361789735181 39.0 38.0 39.0 35.0 39.0 14-15 39.22418844873917 41.0 39.0 41.0 36.0 41.0 16-17 39.18648382717383 40.5 39.0 41.0 36.0 41.0 18-19 39.16947154052236 40.0 39.0 41.0 36.0 41.0 20-21 39.14839503984465 40.0 39.0 41.0 36.0 41.0 22-23 39.052402223434925 40.0 39.0 41.0 36.0 41.0 24-25 38.96998283012468 40.0 38.0 41.0 35.0 41.0 26-27 38.81220409097633 40.0 38.0 41.0 35.0 41.0 28-29 38.63519473278973 40.0 38.0 41.0 34.0 41.0 30-31 38.48001742153123 40.0 38.0 41.0 34.0 41.0 32-33 38.66390294481961 40.0 38.0 41.0 35.0 41.0 34-35 38.64121393720184 40.0 38.0 41.0 34.0 41.0 36-37 38.582616986588825 40.0 38.0 41.0 34.0 41.0 38-39 38.496862082205624 40.0 38.0 41.0 34.0 41.0 40-41 38.32150919842849 40.0 38.0 41.0 34.0 41.0 42-43 38.23741768176079 40.0 38.0 41.0 33.0 41.0 44-45 38.17975142412658 40.0 38.0 41.0 33.0 41.0 46-47 38.08799727424831 40.0 38.0 41.0 33.0 41.0 48-49 38.01557362477132 40.0 37.0 41.0 33.0 41.0 50-51 38.05543077214918 40.0 37.0 41.0 33.0 41.0 52-53 37.959292978794316 40.0 37.0 41.0 33.0 41.0 54-55 37.723754067497126 40.0 37.0 41.0 33.0 41.0 56-57 37.5693190254703 39.5 36.5 41.0 32.0 41.0 58-59 37.37550200292071 39.0 36.0 41.0 32.0 41.0 60-61 37.15993447574317 39.0 36.0 41.0 32.0 41.0 62-63 36.86408674619726 39.0 35.0 40.5 31.0 41.0 64-65 36.5335941838443 38.0 35.0 40.0 31.0 41.0 66-67 36.16921878542527 37.5 35.0 40.0 31.0 41.0 68-69 35.80763992054153 37.0 35.0 39.5 30.0 41.0 70-71 35.38816301425234 36.5 34.0 39.0 30.0 41.0 72-73 34.98133789191141 36.0 34.0 39.0 29.5 40.0 74-75 34.59357535766813 35.5 34.0 38.5 29.0 40.0 76-77 33.96305092468506 35.0 33.0 37.0 29.0 39.0 78-79 33.81300088565525 35.0 34.0 37.0 29.0 39.0 80-81 33.509738563063266 35.0 33.5 36.0 29.0 38.5 82-83 33.156790743829504 35.0 33.0 36.0 29.0 37.0 84-85 32.86888131195688 35.0 33.0 35.5 28.0 37.0 86-87 32.53296495729052 35.0 33.0 35.0 27.0 36.5 88-89 32.31958330756512 35.0 33.0 35.0 27.0 36.0 90-91 32.06016089085498 35.0 32.5 35.0 26.0 36.0 92-93 31.778603398789517 35.0 32.0 35.0 25.5 36.0 94-95 31.49744196106021 34.0 32.0 35.0 25.0 35.0 96-97 31.25549464646886 34.0 32.0 35.0 25.0 35.0 98-99 30.892128532880175 34.0 32.0 35.0 24.0 35.0 100-101 29.19024990126121 33.0 29.5 34.5 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4405916650437902 1101 2 0.42124594534167414 1101 3 0.3695645526957847 1101 4 0.23961429609923357 1101 5 0.2753395545000501 1101 6 0.2502501834863864 1101 7 0.270125401312697 1101 8 0.24988836914917556 1101 9 0.34400706638053435 1101 10-11 0.32232007609248114 1101 12-13 0.35310084534560815 1101 14-15 0.45799627898289685 1101 16-17 0.4030782814587468 1101 18-19 0.3740868846990253 1101 20-21 0.38557319271408375 1101 22-23 0.39914428409758074 1101 24-25 0.41584915532299505 1101 26-27 0.42614918419063486 1101 28-29 0.43367451550372493 1101 30-31 0.42925768272682774 1101 32-33 0.4132226215804522 1101 34-35 0.416554104981266 1101 36-37 0.3992812555092513 1101 38-39 0.3891996609812409 1101 40-41 0.38492757302179825 1101 42-43 0.4126337480020723 1101 44-45 0.34682061113129237 1101 46-47 0.35465986124397375 1101 48-49 0.3866451247723788 1101 50-51 0.4730144922060262 1101 52-53 0.4541067146387405 1101 54-55 0.5350532181873646 1101 56-57 0.4230962835343206 1101 58-59 0.4231190988483604 1101 60-61 0.3507681976210577 1101 62-63 0.40406530512597527 1101 64-65 0.40977088522503635 1101 66-67 0.3282581646670195 1101 68-69 0.2904355978325981 1101 70-71 0.31091949412176234 1101 72-73 0.27305340631901487 1101 74-75 0.25550952672909943 1101 76-77 0.2518499277124562 1101 78-79 0.28839974767520715 1101 80-81 0.2643091506228359 1101 82-83 0.08761698074392399 1101 84-85 0.13824517095916633 1101 86-87 0.3162363398714234 1101 88-89 0.3061251066014492 1101 90-91 0.20484884550489113 1101 92-93 0.07809346599265865 1101 94-95 0.2110880520241487 1101 96-97 0.254091341643349 1101 98-99 0.3381727508197052 1101 100-101 0.37252687396984996 1102 1 0.3750112220214561 1102 2 0.33647445026399936 1102 3 0.27480347021735696 1102 4 0.16707555608656577 1102 5 0.2145836992288963 1102 6 0.21726744348863747 1102 7 0.21239506967023658 1102 8 0.2029554896787431 1102 9 0.26286623892048055 1102 10-11 0.26389604355232166 1102 12-13 0.24639656159364165 1102 14-15 0.3290754808956393 1102 16-17 0.32545111729599085 1102 18-19 0.3328090218182709 1102 20-21 0.30224522739565884 1102 22-23 0.29178588165611785 1102 24-25 0.3298559982107321 1102 26-27 0.331880971368804 1102 28-29 0.3834946557165182 1102 30-31 0.36018614047443265 1102 32-33 0.32236957051611626 1102 34-35 0.38215332562647575 1102 36-37 0.36981010522132607 1102 38-39 0.3617068849182843 1102 40-41 0.38680495494804745 1102 42-43 0.3685736583327923 1102 44-45 0.33595465695419335 1102 46-47 0.27300337920463136 1102 48-49 0.3509793589015615 1102 50-51 0.4577465449506377 1102 52-53 0.4590065282171025 1102 54-55 0.5189458238284317 1102 56-57 0.4311924340431048 1102 58-59 0.43712251356841136 1102 60-61 0.39583302017399546 1102 62-63 0.4464432981838513 1102 64-65 0.44743400662153476 1102 66-67 0.41367687056586533 1102 68-69 0.37921593871173087 1102 70-71 0.4524610903355395 1102 72-73 0.4240136294635306 1102 74-75 0.3976491259382655 1102 76-77 0.41339235483358294 1102 78-79 0.36487742659808475 1102 80-81 0.40941236644535195 1102 82-83 0.3026713509436263 1102 84-85 0.26332617583781825 1102 86-87 0.47373762107854134 1102 88-89 0.4746781182455422 1102 90-91 0.42885712591634473 1102 92-93 0.31983395605426423 1102 94-95 0.42820614481838604 1102 96-97 0.40302670048637523 1102 98-99 0.5048564049529709 1102 100-101 0.6679541939682245 1103 1 0.272464032865507 1103 2 0.2832519330953147 1103 3 0.21936150582246228 1103 4 0.15258568089219438 1103 5 0.17492213444751314 1103 6 0.14599204092407092 1103 7 0.1779552312184265 1103 8 0.15988404147530133 1103 9 0.14940001249782142 1103 10-11 0.13285674970343564 1103 12-13 0.19399067277171866 1103 14-15 0.24990684543073627 1103 16-17 0.2658123601243929 1103 18-19 0.2215176987030958 1103 20-21 0.24570152557502212 1103 22-23 0.20072333193686376 1103 24-25 0.1757775857495858 1103 26-27 0.2017273803538231 1103 28-29 0.2504680135068895 1103 30-31 0.21109973325442155 1103 32-33 0.16261527115942442 1103 34-35 0.22235106963766782 1103 36-37 0.2557448937041542 1103 38-39 0.22729386686379627 1103 40-41 0.20901053040257267 1103 42-43 0.2763550022445713 1103 44-45 0.1826383531651885 1103 46-47 0.1761892333446866 1103 48-49 0.2886840905711736 1103 50-51 0.3151256694765152 1103 52-53 0.28419925595614615 1103 54-55 0.3660271009350211 1103 56-57 0.319612000381305 1103 58-59 0.2519694879292871 1103 60-61 0.2136139579612788 1103 62-63 0.24258731141159728 1103 64-65 0.23875974606880135 1103 66-67 0.2739969200755752 1103 68-69 0.2397575706733832 1103 70-71 0.28745347933622156 1103 72-73 0.3204974414149362 1103 74-75 0.31272844342677786 1103 76-77 0.25242882938870537 1103 78-79 0.30204710346834673 1103 80-81 0.3308317736627302 1103 82-83 0.2655850500927386 1103 84-85 0.28714504213097314 1103 86-87 0.4703267834537499 1103 88-89 0.4288880605543568 1103 90-91 0.29938695534347204 1103 92-93 0.2493451283151238 1103 94-95 0.34746019290283314 1103 96-97 0.40083739610381386 1103 98-99 0.4666498537690309 1103 100-101 0.6368048768505012 1104 1 0.39155463385380074 1104 2 0.3679508723859968 1104 3 0.288597389666279 1104 4 0.1792306722303607 1104 5 0.22181053748720103 1104 6 0.19902261388891418 1104 7 0.22232756719165536 1104 8 0.22738386134743394 1104 9 0.2627204841922861 1104 10-11 0.24057048318698548 1104 12-13 0.2539331743949518 1104 14-15 0.34869918718383275 1104 16-17 0.2887368552411047 1104 18-19 0.3404213457124001 1104 20-21 0.32705337864845063 1104 22-23 0.3444454498969449 1104 24-25 0.3323253659982157 1104 26-27 0.3567947388378201 1104 28-29 0.3650742502645201 1104 30-31 0.38845208280158516 1104 32-33 0.339779188427336 1104 34-35 0.3716566481986092 1104 36-37 0.367337173592027 1104 38-39 0.36597243579223004 1104 40-41 0.3643972121197976 1104 42-43 0.39002272407989835 1104 44-45 0.3166323579676984 1104 46-47 0.3348600135844535 1104 48-49 0.38765090979474337 1104 50-51 0.39335832107470026 1104 52-53 0.42047712056769626 1104 54-55 0.4017424413430746 1104 56-57 0.2961006529069081 1104 58-59 0.34334838203675133 1104 60-61 0.21442953189997382 1104 62-63 0.2288958855933032 1104 64-65 0.2604558047798733 1104 66-67 0.16012929919207153 1104 68-69 0.1558696978023093 1104 70-71 0.2879228993239593 1104 72-73 0.2636532617884626 1104 74-75 0.2700789825661758 1104 76-77 0.16985063373611098 1104 78-79 0.19707192729402578 1104 80-81 0.2996232365274025 1104 82-83 0.14574038801821132 1104 84-85 0.14412405519129834 1104 86-87 0.3506566783839773 1104 88-89 0.3616131861137504 1104 90-91 0.2976258000342753 1104 92-93 0.1956888798880776 1104 94-95 0.32113890782854426 1104 96-97 0.3555812008222752 1104 98-99 0.4247617780647879 1104 100-101 0.6114903142628485 1105 1 -0.0860504639240176 1105 2 0.05687457906737592 1105 3 0.04998062700526873 1105 4 0.03424724139759405 1105 5 0.019166395204869957 1105 6 0.03306188478871519 1105 7 0.06852035620166674 1105 8 -0.012007157421898285 1105 9 -0.01026708111739083 1105 10-11 -0.034969715870360574 1105 12-13 0.02786417814073161 1105 14-15 0.03999683163080903 1105 16-17 0.03616861379573777 1105 18-19 -0.008361174929191861 1105 20-21 0.0041484128871829284 1105 22-23 0.05697288809056289 1105 24-25 -0.009348593522815918 1105 26-27 -0.0031866127578581427 1105 28-29 -0.0035477854806771347 1105 30-31 0.021884650632550517 1105 32-33 0.07003702803528 1105 34-35 0.05466596892748754 1105 36-37 0.0996211405427303 1105 38-39 0.08589833790799872 1105 40-41 0.1303454752700688 1105 42-43 0.12901161018665164 1105 44-45 0.08898205849125418 1105 46-47 -0.13107652405795278 1105 48-49 0.11749621897588014 1105 50-51 0.10922684224613732 1105 52-53 0.09449229157823424 1105 54-55 0.11830399891741905 1105 56-57 0.016035154570452903 1105 58-59 0.12720722978259857 1105 60-61 0.15529158801758314 1105 62-63 0.1827752857373497 1105 64-65 0.19406387000950076 1105 66-67 0.2151450552939309 1105 68-69 0.19599171128688653 1105 70-71 0.08939319149386904 1105 72-73 0.14429447829845543 1105 74-75 0.199432148800625 1105 76-77 0.1854441935636686 1105 78-79 0.249477517773677 1105 80-81 0.33420294803602246 1105 82-83 0.2440933875347966 1105 84-85 0.19037744677905266 1105 86-87 0.3923599429914546 1105 88-89 0.3413887974362879 1105 90-91 0.309639384067502 1105 92-93 0.17870730063409823 1105 94-95 0.3036709623389733 1105 96-97 0.3773308101562023 1105 98-99 0.4967170929389617 1105 100-101 0.6920202165971183 1106 1 -0.16775856008601409 1106 2 -0.037740215561207435 1106 3 -0.04086873381152856 1106 4 -0.024302122352871436 1106 5 -0.37282803769431183 1106 6 -0.25381143380226234 1106 7 -0.14080915226065116 1106 8 -0.3403406665111248 1106 9 -0.48788163761656733 1106 10-11 -0.13332000237889474 1106 12-13 -0.08314477034481627 1106 14-15 -0.2720734432381775 1106 16-17 -0.13194436129688114 1106 18-19 -0.2332390021212447 1106 20-21 -0.17852725776689482 1106 22-23 -0.14320871795883505 1106 24-25 -0.12256073061662676 1106 26-27 -0.10352001771502017 1106 28-29 -0.15250710182462512 1106 30-31 -0.1724141375504118 1106 32-33 -0.1878963602184669 1106 34-35 -0.13436572977035866 1106 36-37 -0.08893794264054833 1106 38-39 -0.12119319285179841 1106 40-41 -0.089804839623703 1106 42-43 -0.17033620463906374 1106 44-45 -0.1428719864182071 1106 46-47 -0.23690184860507912 1106 48-49 -0.10682618921281772 1106 50-51 -0.09341268348254772 1106 52-53 -0.16991190652957755 1106 54-55 -0.12028780525510996 1106 56-57 -0.2157179033837977 1106 58-59 -0.16078396762245006 1106 60-61 -0.25413524031278456 1106 62-63 -0.18446625862039667 1106 64-65 -0.16460023458559903 1106 66-67 -0.1882836341987968 1106 68-69 -0.2300367976397979 1106 70-71 -0.1820823954845494 1106 72-73 -0.14248479635567435 1106 74-75 -0.17959239544320837 1106 76-77 -0.259552597900246 1106 78-79 -0.18286065267887608 1106 80-81 -0.1556380523506391 1106 82-83 -0.24971000954902678 1106 84-85 -0.2275885818540928 1106 86-87 -0.021405255598160977 1106 88-89 0.011485204166604035 1106 90-91 -0.10141324173869037 1106 92-93 -0.2960714530954256 1106 94-95 -0.1744705803537201 1106 96-97 -0.0915196234322515 1106 98-99 0.04763620373573829 1106 100-101 0.019408210540543536 1107 1 -0.179874915543067 1107 2 -0.13742258703761934 1107 3 -0.18417184066903047 1107 4 -0.12050620690822456 1107 5 -0.13229729042962646 1107 6 -0.1551849850528697 1107 7 -0.145182359665867 1107 8 -0.11925237092778218 1107 9 -0.23497860019190142 1107 10-11 -0.2647992458067989 1107 12-13 -0.2662956013422928 1107 14-15 -0.2695293052789296 1107 16-17 -0.2855211838322873 1107 18-19 -0.2778554488090208 1107 20-21 -0.25845721590736304 1107 22-23 -0.3143550631185619 1107 24-25 -0.39489001166752047 1107 26-27 -0.41228543123504124 1107 28-29 -0.39593655383004034 1107 30-31 -0.4853325112781377 1107 32-33 -0.49583721286834503 1107 34-35 -0.41169454300322883 1107 36-37 -0.3597047231101911 1107 38-39 -0.3120918030344981 1107 40-41 -0.3171134068591712 1107 42-43 -0.3432686236783411 1107 44-45 -0.41356184199339197 1107 46-47 -0.4276247696095439 1107 48-49 -0.3039885610919484 1107 50-51 -0.21922100073893347 1107 52-53 -0.26696312774261344 1107 54-55 -0.37939915933366564 1107 56-57 -0.4646621727342435 1107 58-59 -0.3906374934012504 1107 60-61 -0.45000806036463104 1107 62-63 -0.3198001422580745 1107 64-65 -0.31437781666968334 1107 66-67 -0.38702926834808693 1107 68-69 -0.3998773801818416 1107 70-71 -0.3459382854256461 1107 72-73 -0.30256213976016255 1107 74-75 -0.3041550283852743 1107 76-77 -0.41755269319498467 1107 78-79 -0.37126768027323465 1107 80-81 -0.3245541445093778 1107 82-83 -0.33212736499513085 1107 84-85 -0.4024008505133594 1107 86-87 -0.24611227498887445 1107 88-89 -0.2601303316387984 1107 90-91 -0.44530296433695327 1107 92-93 -0.5164828041977572 1107 94-95 -0.4805406429357397 1107 96-97 -0.5824354951176751 1107 98-99 -0.5841119359764555 1107 100-101 -0.4273965410725822 1108 1 -0.36190335949686414 1108 2 -0.2670780988023509 1108 3 -0.2921592564466948 1108 4 -0.1969066612990389 1108 5 -0.36489555757677294 1108 6 -0.5426581624459033 1108 7 -0.7170309765991263 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2305 14-15 0.1543038649114905 2305 16-17 0.07450844845308069 2305 18-19 0.1159058994223301 2305 20-21 0.13251557007681924 2305 22-23 0.15846247597459495 2305 24-25 0.10401976483410635 2305 26-27 0.1541585749212402 2305 28-29 0.1303075986602309 2305 30-31 0.18115979372654323 2305 32-33 0.16118151660867852 2305 34-35 0.13364525764333024 2305 36-37 0.20510177278100628 2305 38-39 0.16110718446845596 2305 40-41 0.2238331632322641 2305 42-43 0.27802853011282735 2305 44-45 0.2633595334298704 2305 46-47 0.3211372052987187 2305 48-49 0.2875232108579766 2305 50-51 0.21741232324998805 2305 52-53 0.2555074739815524 2305 54-55 0.2527431547470016 2305 56-57 0.26282511620625115 2305 58-59 0.30349596230678344 2305 60-61 0.4113249020483565 2305 62-63 0.3627346933058462 2305 64-65 0.3413352744154565 2305 66-67 0.4713137895971897 2305 68-69 0.48012461335093093 2305 70-71 0.4791853441992515 2305 72-73 0.4584771051766836 2305 74-75 0.41900627720819017 2305 76-77 0.39532533067259834 2305 78-79 0.4338577520320257 2305 80-81 0.5033045992679561 2305 82-83 0.5046261220499062 2305 84-85 0.5256255529288794 2305 86-87 0.6207353605836232 2305 88-89 0.5685518480676137 2305 90-91 0.594392806809033 2305 92-93 0.7377356133612913 2305 94-95 0.8335176240449051 2305 96-97 0.8391878747608565 2305 98-99 0.9104518190216133 2305 100-101 1.0375097237205821 2306 1 -0.5537586233545575 2306 2 -0.9616497541867908 2306 3 -0.7497918415834448 2306 4 -0.35636763926694925 2306 5 -0.23907676655317545 2306 6 -0.20427361472266625 2306 7 -0.07753045389429047 2306 8 -0.06363545130506054 2306 9 -0.06615279416385533 2306 10-11 -0.3473579932076092 2306 12-13 -0.27990927733657145 2306 14-15 -0.16083047718489496 2306 16-17 -0.21678960143314185 2306 18-19 -0.17092834441692162 2306 20-21 -0.13905913663675307 2306 22-23 -0.07058761583291329 2306 24-25 -0.12019080784556735 2306 26-27 -0.14950697766057175 2306 28-29 -0.12341654486829867 2306 30-31 -0.059399989779038265 2306 32-33 -0.10789701683375341 2306 34-35 -0.21494914000456333 2306 36-37 -0.22135780038915698 2306 38-39 -0.12371742162816446 2306 40-41 -0.20460518690381946 2306 42-43 -0.1262355543854028 2306 44-45 -0.19109996836855458 2306 46-47 -0.12641052898711536 2306 48-49 -0.07722419468044706 2306 50-51 -0.04540688014984795 2306 52-53 -0.04485176462840457 2306 54-55 -0.11735925826395288 2306 56-57 -0.12278277213079036 2306 58-59 -0.15918052616773082 2306 60-61 -0.20861981332598134 2306 62-63 -0.036449602177668794 2306 64-65 -0.0852461337446826 2306 66-67 -0.04535532982060886 2306 68-69 0.007498197622219038 2306 70-71 0.027508151572419592 2306 72-73 -0.0363055006820332 2306 74-75 -0.015333503736883358 2306 76-77 -0.0056654002821190375 2306 78-79 0.0453893613334202 2306 80-81 0.17467340258099995 2306 82-83 0.18604758264193322 2306 84-85 0.061943122101595804 2306 86-87 0.2332937465252698 2306 88-89 0.10385703235080967 2306 90-91 0.19095999676975595 2306 92-93 0.2562841596745322 2306 94-95 0.4073724295138028 2306 96-97 0.240266573287375 2306 98-99 0.3675297169386482 2306 100-101 0.6815889769370145 2307 1 -1.6414349029298698 2307 2 -2.1837592496671796 2307 3 -1.7517544815363735 2307 4 -0.9198537349362397 2307 5 -0.5229441975089557 2307 6 -0.4165184245882685 2307 7 -0.34812294750142314 2307 8 -0.4762441888185194 2307 9 -0.4236332344724971 2307 10-11 -0.34748243438063753 2307 12-13 -0.33962070750745 2307 14-15 -0.6551606383124025 2307 16-17 -0.5499998611799484 2307 18-19 -0.3996199397161675 2307 20-21 -0.40323004092557113 2307 22-23 -0.46212879463225676 2307 24-25 -0.4237184998951946 2307 26-27 -0.3861379390148727 2307 28-29 -0.33551196788354076 2307 30-31 -0.7429738136082875 2307 32-33 -0.47002536342090906 2307 34-35 -0.45381011980539654 2307 36-37 -0.37096813155383046 2307 38-39 -0.3848148479818363 2307 40-41 -0.3547099087672976 2307 42-43 -0.3697283094854953 2307 44-45 -0.3133388957928176 2307 46-47 -0.3878846589858682 2307 48-49 -0.3628958095346988 2307 50-51 -0.3041283794554204 2307 52-53 -0.3520454981941299 2307 54-55 -0.3315821501579279 2307 56-57 -0.25056878782857694 2307 58-59 -0.3971593063725152 2307 60-61 -0.3646437623536869 2307 62-63 -0.30248939928434027 2307 64-65 -0.27007417136297107 2307 66-67 -0.3548368669622448 2307 68-69 -0.2145804290389961 2307 70-71 -0.2891292840058952 2307 72-73 -0.3404841679969479 2307 74-75 -0.35570870863797666 2307 76-77 -0.2982164996050827 2307 78-79 -0.325176882585275 2307 80-81 -0.2837792999476818 2307 82-83 -0.2397596750022828 2307 84-85 -0.26314605021131143 2307 86-87 -0.2526420919701948 2307 88-89 -0.2622517616415152 2307 90-91 -0.2797216857596183 2307 92-93 -0.22788212102712535 2307 94-95 -0.17686657060719213 2307 96-97 -0.2157628691861042 2307 98-99 -0.2051744289697588 2307 100-101 0.012887112596278172 2308 1 -1.280322150207109 2308 2 -1.2393967517962494 2308 3 -0.7861623641952811 2308 4 -0.5063565079829004 2308 5 -0.6775013650601451 2308 6 -0.5785538209990193 2308 7 -1.4691126922332174 2308 8 -0.5825465346015619 2308 9 -0.5698483267634344 2308 10-11 -0.8121353153405124 2308 12-13 -0.8915671453571861 2308 14-15 -0.6675923643144088 2308 16-17 -0.8625071109745122 2308 18-19 -0.5938358394987233 2308 20-21 -0.6819621094320141 2308 22-23 -0.5208144582260417 2308 24-25 -0.5261780347985905 2308 26-27 -0.5042539459834359 2308 28-29 -1.3018874364887836 2308 30-31 -0.6456974369233919 2308 32-33 -0.4824699671075976 2308 34-35 -1.134851827911426 2308 36-37 -0.6577503502596542 2308 38-39 -0.48955265213356114 2308 40-41 -0.9786916060231547 2308 42-43 -0.45743286838806796 2308 44-45 -0.537608375257193 2308 46-47 -0.4803820676634487 2308 48-49 -0.38313694230334505 2308 50-51 -0.501896109032721 2308 52-53 -0.5709145739142656 2308 54-55 -1.0315403040382733 2308 56-57 -1.709689091657502 2308 58-59 -1.160439294303501 2308 60-61 -0.7739060579047532 2308 62-63 -0.4962950538115365 2308 64-65 -0.3499662422746397 2308 66-67 -0.38793326055389343 2308 68-69 -0.29785234185663256 2308 70-71 -0.20670278837962996 2308 72-73 -0.2956956230282586 2308 74-75 -0.162240487090358 2308 76-77 -1.157859955703934 2308 78-79 -0.6491783158364015 2308 80-81 -0.18074425501919222 2308 82-83 -0.09490209011438111 2308 84-85 -2.1070810342393997E-4 2308 86-87 -0.34038250816450244 2308 88-89 -0.3133483422625929 2308 90-91 -0.9441957031777903 2308 92-93 -0.09872746206979954 2308 94-95 0.10391631032437942 2308 96-97 -0.02427409232725708 2308 98-99 -0.5299922910730075 2308 100-101 0.18782383447777207 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 6.0 11 23.0 12 197.0 13 1097.0 14 4057.0 15 9029.0 16 15725.0 17 23691.0 18 34546.0 19 45329.0 20 49382.0 21 51815.0 22 56431.0 23 63684.0 24 76175.0 25 93796.0 26 117975.0 27 149599.0 28 188903.0 29 237285.0 30 296978.0 31 370884.0 32 468480.0 33 611618.0 34 840919.0 35 1237953.0 36 2096297.0 37 4046496.0 38 5068182.0 39 1028376.0 40 579.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.161351497547543 30.009708422064822 26.603652019686326 21.22528806070131 2 23.207178333097104 29.391623542479618 27.8305790741871 19.570619050236182 3 22.388604201948006 27.984573623413763 27.97941321662329 21.647408958014942 4 23.58897893428351 27.760729106783792 27.478775682178085 21.17151627675461 5 23.301538103568497 28.55192503176216 26.886223238924956 21.260313625744388 6 23.001784095774568 27.758960150836188 26.329548794837198 22.90970695855204 7 23.62751639277922 27.731029237383666 26.895149792250816 21.746304577586297 8 23.022315746943377 27.718429086285983 26.987591396653855 22.27166377011678 9 22.35967391642027 27.361025626844498 27.37083152955826 22.90846892717697 10-11 22.733229635671083 27.776888465001342 27.192922371325295 22.296959528002276 12-13 22.618468755356727 27.731500730640995 27.405354670823368 22.244675843178914 14-15 22.60945831672742 27.669327257800425 27.39550248656288 22.325711938909283 16-17 22.72014063573605 27.96648371378404 27.303358819616918 22.010016830862988 18-19 22.37146124785348 27.595638357613694 27.639932690432513 22.392967704100318 20-21 22.397783877557075 27.82818866695666 27.28603795075262 22.487989504733648 22-23 22.452792388444262 27.815808353205956 27.644028607318262 22.087370651031527 24-25 22.465153460379288 27.482467390016513 27.943874242833765 22.108504906770435 26-27 22.498596656725198 27.450690338443646 27.949984342374222 22.100728662456937 28-29 22.496080097621665 27.532718594832073 27.934546553942557 22.0366547536037 30-31 22.51255343566145 27.48232956082804 27.907801605125034 22.09731539838548 32-33 22.563795785683347 27.502190129569236 27.864372158710765 22.069641926036653 34-35 22.593595316585162 27.52627099685303 27.868230882669508 22.0119028038923 36-37 22.584845211656216 27.528561933416242 27.804885908177297 22.081706946750245 38-39 22.6187580150238 27.511747268969316 27.75055137231439 22.118943343692493 40-41 22.680743469080774 27.63223259809504 27.477886532341806 22.209137400482383 42-43 22.705463600228793 27.670365700005213 27.469382298129872 22.15478840163612 44-45 22.688209261087916 27.707813256504423 27.45205275147556 22.151924730932105 46-47 22.689649774229935 27.788545629584366 27.4254292917182 22.096375304467493 48-49 22.708325035196395 27.751278216811293 27.402399229666774 22.13799751832554 50-51 22.678114098707084 27.761060176019136 27.435452139182264 22.125373586091516 52-53 22.6848220303865 27.806930974023498 27.40817205998065 22.100074935609353 54-55 22.68938076273956 27.769749536417994 27.369550109233128 22.17131959160932 56-57 22.677714920366522 27.801883392833084 27.3711931041421 22.149208582658293 58-59 22.694555618183486 27.871589187404222 27.373952641365975 22.059902553046317 60-61 22.68938076273956 27.814040976640143 27.353666860914178 22.142911399706126 62-63 22.721398915287818 27.83498048393952 27.336910048400664 22.106710552371997 64-65 22.716848860724767 27.881415338294673 27.35579581206383 22.045939988916725 66-67 22.683737306634974 27.8672242590281 27.351662291421363 22.097376142915564 68-69 22.684324503759132 27.91975381456847 27.27773041311429 22.118191268558103 70-71 22.71773688790268 27.96646057301067 27.273108043634476 22.04269449545217 72-73 22.689493574009717 27.94207598307646 27.264965384006384 22.103465058907442 74-75 22.685898076348007 27.971762702708112 27.275011372243814 22.067327848700067 76-77 22.66961630542906 28.06954195990728 27.21084279480037 22.04999893986329 78-79 22.658655600903117 28.032527480970042 27.2222185904064 22.08659832772044 80-81 22.665826348049844 28.054511215667553 27.203789857017213 22.075872579265393 82-83 22.650799308345427 28.144094934559917 27.212482110012743 21.992623647081917 84-85 22.632642479043284 28.114162344211252 27.230795139535104 22.022400037210364 86-87 22.635610283227443 28.210650691356637 27.170663839944066 21.98307518547185 88-89 22.639839259560045 28.225645912497676 27.174493637936102 21.96002119000617 90-91 22.655696474508964 28.234181965272988 27.121943834219035 21.988177725999012 92-93 22.628018526041412 28.231617865279734 27.147487033356153 21.9928765753227 94-95 22.664640768801398 28.314837440816376 27.08311464094091 21.937407149441313 96-97 22.58529421744165 28.31657550792625 27.119734674827146 21.97839559980496 98-99 22.594190259579044 28.389878444688815 27.046041551981226 21.969889743750915 100-101 22.700598501703567 28.52716768704032 26.746990454078905 22.025243357177214 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3190.0 1 1860.0 2 424.0 3 292.5 4 256.5 5 263.0 6 278.0 7 290.5 8 305.5 9 336.5 10 352.5 11 377.0 12 472.0 13 620.5 14 858.5 15 1233.5 16 2394.5 17 3485.5 18 4179.5 19 5495.5 20 8787.5 21 13590.5 22 19296.5 23 27008.5 24 35249.0 25 46419.0 26 61861.0 27 81043.0 28 103643.5 29 134696.5 30 176784.5 31 221702.5 32 272204.5 33 338516.5 34 397674.5 35 456037.0 36 527469.5 37 586443.5 38 629633.5 39 668921.0 40 704535.5 41 712644.0 42 706300.0 43 712637.0 44 715091.5 45 713463.5 46 699451.0 47 680691.5 48 663904.0 49 638728.0 50 606315.0 51 572971.0 52 551981.5 53 526510.5 54 486930.5 55 438994.5 56 388993.5 57 342128.0 58 302817.5 59 262793.5 60 213151.0 61 170306.0 62 138461.0 63 107354.5 64 83242.5 65 66009.0 66 49849.0 67 36580.5 68 28381.0 69 23178.0 70 19701.0 71 17618.5 72 16161.0 73 13726.0 74 10729.5 75 8626.0 76 6969.5 77 5024.0 78 3324.5 79 2296.5 80 1495.5 81 1060.5 82 809.5 83 555.5 84 344.5 85 210.5 86 90.0 87 11.5 88 3.5 89 2.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044690618562706896 2 9.082753546077646E-4 3 2.776892803896351E-4 4 1.6198541356062047E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.7355580024352193E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.8925966707253656E-6 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 2.8925966707253656E-6 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 5.785193341450731E-6 94-95 1.4462983353626828E-5 96-97 2.8925966707253656E-6 98-99 8.677790012176096E-6 100-101 5.2066740073056575E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7285507E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.28105494022801 #Duplication Level Percentage of deduplicated Percentage of total 1 76.54699391722045 36.9576961882662 2 13.006448034579929 12.559300642695485 3 4.398659523145302 6.371157663010065 4 2.0088786156949023 3.879631152504591 5 1.0545224405111262 2.5456727943010504 6 0.6318311241045542 1.8303283929502805 7 0.43524295269693897 1.4709792238055581 8 0.2805094692931114 1.0834634478555931 9 0.21434407231065372 0.9313882138208591 >10 1.0654658962585024 9.816267530156694 >50 0.1868607010258987 6.457028934473474 >100 0.168493863648155 15.460688009681 >500 0.0016413130801254247 0.45444079454597613 >1k 8.40641784103612E-5 0.11002595674634527 >5k 2.4012251942975217E-5 0.07193105518692611 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.52075134388595E-5 0.0 0.0 0.0 0.0 2 7.52075134388595E-5 5.785193341450731E-6 0.0 5.785193341450731E-6 0.0 3 8.099270678031024E-5 5.785193341450731E-6 0.0 5.785193341450731E-6 5.785193341450731E-6 4 8.099270678031024E-5 5.785193341450731E-6 0.0 5.785193341450731E-6 5.785193341450731E-6 5 8.677790012176097E-5 5.785193341450731E-6 0.0 5.785193341450731E-6 5.785193341450731E-6 6 8.677790012176097E-5 5.785193341450731E-6 0.0 1.1570386682901462E-5 5.785193341450731E-6 7 8.677790012176097E-5 5.785193341450731E-6 0.0 1.7355580024352193E-5 5.785193341450731E-6 8 9.834828680466243E-5 5.785193341450731E-6 0.0 1.7355580024352193E-5 1.1570386682901462E-5 9 1.0991867348756388E-4 5.785193341450731E-6 0.0 2.8925966707253653E-5 1.1570386682901462E-5 10-11 1.0991867348756388E-4 5.785193341450731E-6 0.0 4.3388950060880484E-5 1.1570386682901462E-5 12-13 1.0991867348756388E-4 1.1570386682901462E-5 0.0 5.785193341450731E-5 1.1570386682901462E-5 14-15 1.2148906017046535E-4 2.3140773365802925E-5 0.0 6.942232009740877E-5 1.1570386682901462E-5 16-17 1.3884464019481754E-4 2.3140773365802925E-5 0.0 7.52075134388595E-5 2.6033370036528287E-5 18-19 1.4752243020699365E-4 2.3140773365802925E-5 0.0 7.810011010958486E-5 3.181856337797902E-5 20-21 1.5330762354844437E-4 2.3140773365802925E-5 0.0 8.677790012176097E-5 3.4711160048704386E-5 22-23 1.6198541356062047E-4 2.3140773365802925E-5 0.0 8.677790012176097E-5 3.4711160048704386E-5 24-25 1.7355580024352193E-4 2.3140773365802925E-5 0.0 8.677790012176097E-5 3.4711160048704386E-5 26-27 1.9091138026787414E-4 3.181856337797902E-5 0.0 8.967049679248633E-5 3.4711160048704386E-5 28-29 2.1405215363367705E-4 3.4711160048704386E-5 0.0 9.545569013393707E-5 3.4711160048704386E-5 30-31 2.256225403165785E-4 3.4711160048704386E-5 0.0 9.834828680466243E-5 3.4711160048704386E-5 32-33 2.343003303287546E-4 3.4711160048704386E-5 0.0 9.834828680466243E-5 4.049635339015512E-5 34-35 2.603337003652829E-4 3.4711160048704386E-5 0.0 9.834828680466243E-5 4.049635339015512E-5 36-37 2.7190408704818437E-4 3.4711160048704386E-5 0.0 1.0413348014611316E-4 4.049635339015512E-5 38-39 2.9793745708471265E-4 3.4711160048704386E-5 0.0 1.0413348014611316E-4 4.049635339015512E-5 40-41 3.32648617133417E-4 3.760375671942975E-5 0.0 1.0702607681683852E-4 4.049635339015512E-5 42-43 3.5000419715776923E-4 4.049635339015512E-5 0.0 1.1281127015828925E-4 4.049635339015512E-5 44-45 3.6735977718212144E-4 4.3388950060880484E-5 0.0 1.2727425351191608E-4 4.3388950060880484E-5 46-47 3.8182276053574823E-4 4.628154673160585E-5 0.0 1.3884464019481754E-4 4.628154673160585E-5 48-49 4.19426517255178E-4 4.628154673160585E-5 0.0 1.4752243020699365E-4 4.628154673160585E-5 50-51 4.6281546731605846E-4 5.206674007305658E-5 0.0 1.6198541356062047E-4 5.206674007305658E-5 52-53 6.537268475839326E-4 5.206674007305658E-5 0.0 1.7355580024352193E-4 5.206674007305658E-5 54-55 9.574494980100959E-4 5.785193341450731E-5 0.0 1.764483969142473E-4 5.206674007305658E-5 56-57 0.0014694391087284858 5.785193341450731E-5 2.8925966707253656E-6 1.7934099358497265E-4 5.206674007305658E-5 58-59 0.002079777006251538 5.785193341450731E-5 5.785193341450731E-6 1.851261869264234E-4 5.206674007305658E-5 60-61 0.002918630040761894 5.785193341450731E-5 5.785193341450731E-6 1.9669657360932486E-4 5.785193341450731E-5 62-63 0.004127735449125097 5.785193341450731E-5 5.785193341450731E-6 2.0537436362150094E-4 5.785193341450731E-5 64-65 0.00561742273454866 6.363712675595804E-5 5.785193341450731E-6 2.1983734697512776E-4 6.363712675595804E-5 66-67 0.007407940073727661 6.65297234266834E-5 5.785193341450731E-6 2.1983734697512776E-4 6.363712675595804E-5 68-69 0.010025740060734117 6.942232009740877E-5 5.785193341450731E-6 2.256225403165785E-4 6.363712675595804E-5 70-71 0.0134563597122144 6.942232009740877E-5 5.785193341450731E-6 2.3719292699947997E-4 6.363712675595804E-5 72-73 0.01817418488216747 6.942232009740877E-5 5.785193341450731E-6 2.429781203409307E-4 6.363712675595804E-5 74-75 0.024156074797227527 7.52075134388595E-5 5.785193341450731E-6 2.4876331368238144E-4 6.363712675595804E-5 76-77 0.03191401906811296 8.099270678031024E-5 5.785193341450731E-6 2.603337003652829E-4 6.363712675595804E-5 78-79 0.04191372575881054 8.677790012176097E-5 5.785193341450731E-6 2.661188937067336E-4 6.363712675595804E-5 80-81 0.054432884149709926 9.545569013393707E-5 5.785193341450731E-6 2.8058187706036044E-4 6.363712675595804E-5 82-83 0.06962190926768883 9.834828680466243E-5 5.785193341450731E-6 2.950448604139873E-4 6.363712675595804E-5 84-85 0.08905437370162184 9.834828680466243E-5 5.785193341450731E-6 3.095078437676141E-4 6.363712675595804E-5 86-87 0.11253647347457035 9.834828680466243E-5 5.785193341450731E-6 3.1240044043833945E-4 6.363712675595804E-5 88-89 0.14163888857873824 1.0413348014611316E-4 5.785193341450731E-6 3.181856337797902E-4 6.363712675595804E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 11380 0.0 87.778175 1 TCGACGG 11405 0.0 87.58739 2 CGACGGC 12505 0.0 80.22394 3 ACGGCGC 13205 0.0 75.790955 5 GACGGCG 13575 0.0 73.76041 4 CGGCGCG 14725 0.0 68.06415 6 CGCGCCG 18685 0.0 56.155704 9 GGCGCGC 20350 0.0 51.607826 7 GCGCGCC 20600 0.0 51.02764 8 CGCCGGA 18135 0.0 29.67588 10-11 GCCGGAT 18900 0.0 29.454865 12-13 CCGGATC 19340 0.0 28.011093 12-13 CGGATCC 21550 0.0 25.821787 14-15 GCGCCGG 22000 0.0 25.660656 10-11 TATCTCG 2295 0.0 24.422878 2 TCCATAT 22525 0.0 22.584784 18-19 ATCTCGA 5285 0.0 21.220865 1 CCATATT 24595 0.0 21.195757 18-19 GGGCGCG 5150 0.0 21.121246 8 ATCCATA 27465 0.0 19.058699 16-17 >>END_MODULE