FastQCFastQC Report
Tue 31 May 2016
SRR1051290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15476735
Sequences flagged as poor quality0
Sequence length101
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT169147810.929165615357503No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT1180200.7625639387118794No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220920.1427432853247148No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA191320.1236178044012513No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT185380.11977978559431302No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT168600.1089377055302685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1222950.094.28961
ATATCTC2037500.092.90091
TATCTCG2038000.092.791882
TCGACGG1264100.091.212692
ATCTCGA2073250.091.124863
CGACGGC1269850.090.683733
TCTCGAG2056050.090.249474
CTCGAGG2060800.090.085245
GACGGCG1279650.090.026364
ACGGCGC1280450.089.933025
TCGAGGG2068350.089.767886
CGAGGGC2076950.089.352747
CGGCGCG1291550.089.248366
AGGGCGC2083150.089.187119
GAGGGCG2102500.088.4272848
CTCGACG42750.078.098565
TCTCGAC61850.054.4415324
GGGCGCG2088650.044.539810-11
GGATCCT2121150.043.24611718-19
GATCCTT2095600.043.1773520-21