##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051290_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15476735 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08248328862644 33.0 31.0 34.0 30.0 34.0 2 32.346332026748534 34.0 31.0 34.0 30.0 34.0 3 32.41151980698771 34.0 31.0 34.0 30.0 34.0 4 35.833788715772414 37.0 35.0 37.0 35.0 37.0 5 35.82141866485405 37.0 35.0 37.0 35.0 37.0 6 35.82778421934601 37.0 35.0 37.0 35.0 37.0 7 35.768133330447284 37.0 35.0 37.0 35.0 37.0 8 35.75535356779062 37.0 35.0 37.0 33.0 37.0 9 37.43453389878421 39.0 37.0 39.0 34.0 39.0 10-11 37.410821823853674 39.0 37.0 39.0 34.0 39.0 12-13 37.381869722522225 39.0 37.0 39.0 34.0 39.0 14-15 38.75551842814392 40.0 38.0 41.0 34.0 41.0 16-17 38.629900524884604 40.0 38.0 41.0 34.0 41.0 18-19 38.68303140164899 40.0 38.0 41.0 34.0 41.0 20-21 38.64942379642734 40.0 38.0 41.0 34.0 41.0 22-23 38.644795106978314 40.0 38.0 41.0 34.0 41.0 24-25 38.67337413220554 40.0 38.0 41.0 34.0 41.0 26-27 38.64202950428498 40.0 38.0 41.0 34.0 41.0 28-29 38.440402061545925 40.0 38.0 41.0 34.0 41.0 30-31 37.82508885110458 39.5 36.5 41.0 33.0 41.0 32-33 37.16975679948 38.5 35.5 40.5 33.0 41.0 34-35 36.8780240470616 38.0 35.0 40.0 33.0 41.0 36-37 36.99834991036546 38.5 35.0 41.0 33.0 41.0 38-39 36.79713983601839 39.0 35.0 41.0 32.0 41.0 40-41 35.91335972994304 39.0 35.0 41.0 27.0 41.0 42-43 35.97146200409841 39.0 35.0 41.0 27.0 41.0 44-45 35.923453525565954 39.0 35.0 41.0 27.0 41.0 46-47 35.96058955587209 39.0 35.0 41.0 27.5 41.0 48-49 35.717049106287604 39.0 35.0 41.0 24.5 41.0 50-51 35.53012495852646 38.5 34.5 40.5 26.0 41.0 52-53 35.33360615142664 38.0 34.5 40.0 26.0 41.0 54-55 34.97238936377731 38.0 34.5 40.0 24.0 41.0 56-57 34.452616976384235 38.0 34.0 40.0 22.5 41.0 58-59 33.96458897823088 38.0 34.0 40.0 19.0 41.0 60-61 33.62414734115432 38.0 34.0 40.0 17.5 41.0 62-63 33.198029623173106 37.0 33.0 40.0 11.5 41.0 64-65 32.747493964327745 37.0 33.0 40.0 7.0 41.0 66-67 32.1777800033405 36.0 32.5 40.0 2.0 41.0 68-69 31.62028993841401 36.0 31.5 39.0 2.0 41.0 70-71 31.045018862182495 35.0 31.0 39.0 2.0 41.0 72-73 30.53797309316209 35.0 31.0 38.5 2.0 40.0 74-75 30.036886268324682 35.0 30.0 37.0 2.0 39.5 76-77 29.488901825869604 35.0 29.5 37.0 2.0 39.0 78-79 29.068501657487836 35.0 29.0 36.5 2.0 39.0 80-81 28.684879562775997 35.0 29.0 36.0 2.0 38.0 82-83 28.310873094357433 35.0 29.0 36.0 2.0 37.0 84-85 27.926881412649372 35.0 27.5 35.0 2.0 37.0 86-87 27.598537708373243 34.5 26.5 35.0 2.0 36.0 88-89 27.27705749953075 34.0 26.0 35.0 2.0 36.0 90-91 27.035124204168383 34.0 26.0 35.0 2.0 36.0 92-93 26.814993795525993 34.0 25.0 35.0 2.0 35.5 94-95 26.521072403190985 34.0 24.5 35.0 2.0 35.0 96-97 26.219441019052145 34.0 23.5 35.0 2.0 35.0 98-99 25.901885378279076 34.0 19.5 35.0 2.0 35.0 100-101 24.84091890182264 33.0 8.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34816897190953 1101 2 0.33518323252905446 1101 3 0.3512945838277446 1101 4 0.34734660732348743 1101 5 0.3591428222070334 1101 6 0.34067620454984393 1101 7 0.35551153949358394 1101 8 0.2938179827518539 1101 9 0.3884665586670053 1101 10-11 0.3182320379613728 1101 12-13 0.3713071184713854 1101 14-15 0.5257680995657026 1101 16-17 0.41125828098690675 1101 18-19 0.49950938339724615 1101 20-21 0.45292147301378094 1101 22-23 0.4525518838080629 1101 24-25 0.47899999015290007 1101 26-27 0.5266435357920543 1101 28-29 0.5915810554550873 1101 30-31 0.6778314551633784 1101 32-33 0.6279110846923288 1101 34-35 0.6743824392484967 1101 36-37 0.6990444496600858 1101 38-39 0.7208630772339433 1101 40-41 0.7709989273560254 1101 42-43 0.754465014162605 1101 44-45 0.7833637817890704 1101 46-47 0.7724342890976814 1101 48-49 0.7626604311766911 1101 50-51 0.7262783893417577 1101 52-53 0.6730567808015664 1101 54-55 0.6725224216007035 1101 56-57 0.6948778756927609 1101 58-59 0.6617095635517174 1101 60-61 0.6558929695219149 1101 62-63 0.7253274498072741 1101 64-65 0.6592016361867081 1101 66-67 0.6047062941826553 1101 68-69 0.5809534979909436 1101 70-71 0.7191463822085247 1101 72-73 0.7651533685243059 1101 74-75 0.6978656369700111 1101 76-77 0.568860399107205 1101 78-79 0.4782015830575794 1101 80-81 0.5088757203000789 1101 82-83 0.5337570048729923 1101 84-85 0.5572049122928355 1101 86-87 0.572979378211528 1101 88-89 0.6018321576384231 1101 90-91 0.6530960268304007 1101 92-93 0.6171342028751425 1101 94-95 0.6634803668420837 1101 96-97 0.6756769942678815 1101 98-99 0.7277211605332177 1101 100-101 0.9025415481295447 1102 1 0.3472765528447539 1102 2 0.3347989044977666 1102 3 0.36456231424204333 1102 4 0.2805609609740003 1102 5 0.2876961271076297 1102 6 0.29093058573811703 1102 7 0.3267710818358367 1102 8 0.2854522905691397 1102 9 0.358207055766151 1102 10-11 0.3512831418432967 1102 12-13 0.353470306503624 1102 14-15 0.5150106637777938 1102 16-17 0.45567781769623394 1102 18-19 0.4963439928834461 1102 20-21 0.4251184382210411 1102 22-23 0.4035755164222792 1102 24-25 0.448417392059298 1102 26-27 0.4800278329098049 1102 28-29 0.510041133483746 1102 30-31 0.5725900121849321 1102 32-33 0.5529210578792032 1102 34-35 0.6185895900002336 1102 36-37 0.6413482282714611 1102 38-39 0.6817526972970853 1102 40-41 0.6561979264833084 1102 42-43 0.6343516449573201 1102 44-45 0.6254511134976966 1102 46-47 0.5507524651601727 1102 48-49 0.5859918357595291 1102 50-51 0.5837226824391593 1102 52-53 0.5528941903553815 1102 54-55 0.5386264574135851 1102 56-57 0.5248936288939845 1102 58-59 0.49014449433908425 1102 60-61 0.3926166545349119 1102 62-63 0.48464609533362335 1102 64-65 0.4426600525278701 1102 66-67 0.42283524131418915 1102 68-69 0.45329752815548474 1102 70-71 0.5639128513256857 1102 72-73 0.547439886361019 1102 74-75 0.5365930190874444 1102 76-77 0.38234807716082386 1102 78-79 0.30252413269336387 1102 80-81 0.37315460111039656 1102 82-83 0.441847382891531 1102 84-85 0.3721737194484298 1102 86-87 0.3691945625810469 1102 88-89 0.4798452491004568 1102 90-91 0.5669212577673122 1102 92-93 0.4992635561238714 1102 94-95 0.5683591031185884 1102 96-97 0.5389254541876021 1102 98-99 0.6393818339887503 1102 100-101 0.7007319803068732 1103 1 0.24971586232214094 1103 2 0.24435960677774204 1103 3 0.2989854615938583 1103 4 0.2557742946275283 1103 5 0.2844564912257468 1103 6 0.26181742134062347 1103 7 0.30352914576288725 1103 8 0.26577773946286953 1103 9 0.36982454037026 1103 10-11 0.3034875729193729 1103 12-13 0.34788087660079015 1103 14-15 0.4401802262586898 1103 16-17 0.48553029214647836 1103 18-19 0.49065781174095946 1103 20-21 0.42700048294705795 1103 22-23 0.4525207649955121 1103 24-25 0.4457814781592404 1103 26-27 0.4155434772109601 1103 28-29 0.4369096259692924 1103 30-31 0.4844252363278585 1103 32-33 0.44988257301843504 1103 34-35 0.5019791398031899 1103 36-37 0.4456214335595732 1103 38-39 0.4054025232880676 1103 40-41 0.42730573802723626 1103 42-43 0.3991020298234389 1103 44-45 0.37183737449021237 1103 46-47 0.38779389974393297 1103 48-49 0.3877824544571453 1103 50-51 0.32609295866722476 1103 52-53 0.35395014566137917 1103 54-55 0.3507702404936879 1103 56-57 0.3539747669769966 1103 58-59 0.3743255181254881 1103 60-61 0.35516273965724565 1103 62-63 0.4416266717897699 1103 64-65 0.4310149005901849 1103 66-67 0.35270975144478456 1103 68-69 0.37211088347259036 1103 70-71 0.4289585555844049 1103 72-73 0.46084084456132857 1103 74-75 0.4252859727228966 1103 76-77 0.4917073876438458 1103 78-79 0.3900639732784903 1103 80-81 0.3943223314434512 1103 82-83 0.4824012117403029 1103 84-85 0.4134066268649228 1103 86-87 0.450269884395162 1103 88-89 0.5075943526638405 1103 90-91 0.5318712958411673 1103 92-93 0.45848709091841755 1103 94-95 0.4834243665061031 1103 96-97 0.4745949792700621 1103 98-99 0.49835165670768333 1103 100-101 0.6162008402976547 1104 1 0.26388319467048404 1104 2 0.24859877014193188 1104 3 0.1881991914070298 1104 4 0.16894467170015304 1104 5 0.17102357144978697 1104 6 0.16467923282277752 1104 7 0.20337389732871713 1104 8 0.17609176246265434 1104 9 0.19624423934554613 1104 10-11 0.21708872698120985 1104 12-13 0.2825242943454285 1104 14-15 0.3206101683001137 1104 16-17 0.4021095754455075 1104 18-19 0.34487282780796136 1104 20-21 0.3945399930938791 1104 22-23 0.4277080691221542 1104 24-25 0.37390784224807305 1104 26-27 0.33974974666760005 1104 28-29 0.3345824640615902 1104 30-31 0.41651645623948497 1104 32-33 0.37902277051215805 1104 34-35 0.4284435182871036 1104 36-37 0.4225073779418054 1104 38-39 0.3953314303610185 1104 40-41 0.2904114482775455 1104 42-43 0.26821475941089545 1104 44-45 0.25371963137959597 1104 46-47 0.2619227162521369 1104 48-49 0.2988333209900347 1104 50-51 0.22889426752639963 1104 52-53 0.2802439953866269 1104 54-55 0.2889617726686211 1104 56-57 0.26251435939600043 1104 58-59 0.2706419802647275 1104 60-61 0.2064357177956495 1104 62-63 0.34820446837161967 1104 64-65 0.3422736572131697 1104 66-67 0.3682517375353669 1104 68-69 0.37981303653755205 1104 70-71 0.4150484703130708 1104 72-73 0.40007163062304585 1104 74-75 0.39597731750359344 1104 76-77 0.47378009789491315 1104 78-79 0.3190724314619189 1104 80-81 0.3412168789919683 1104 82-83 0.3562320366257197 1104 84-85 0.3342934666290631 1104 86-87 0.3643978982067715 1104 88-89 0.39821472182332585 1104 90-91 0.45682608144739945 1104 92-93 0.3598808603487136 1104 94-95 0.43940173265454874 1104 96-97 0.4716097041782703 1104 98-99 0.46950374627361313 1104 100-101 0.485821170081163 1105 1 0.06519125581793617 1105 2 0.08940290385251615 1105 3 0.10528023633284533 1105 4 -0.03527120876137957 1105 5 0.08134928303125832 1105 6 0.09147500188277746 1105 7 0.08914669001321585 1105 8 0.11175723789311576 1105 9 0.12854582167047823 1105 10-11 0.05419152397143989 1105 12-13 0.1424469403467583 1105 14-15 0.2432344327622573 1105 16-17 0.09222339242402455 1105 18-19 0.16704651845535778 1105 20-21 0.1109624709759558 1105 22-23 0.0990682135534584 1105 24-25 0.12116853650120873 1105 26-27 0.13519941053358764 1105 28-29 0.209625698013987 1105 30-31 0.237568030350495 1105 32-33 0.14323558441153494 1105 34-35 0.2289395810154602 1105 36-37 0.20045432924179352 1105 38-39 0.2089709205229653 1105 40-41 0.05435609897882898 1105 42-43 0.019761577561851595 1105 44-45 0.014659177819012825 1105 46-47 0.07346867348211816 1105 48-49 0.05864127900866123 1105 50-51 0.06165943733869028 1105 52-53 -0.015514328184629278 1105 54-55 0.07521134430879783 1105 56-57 0.16404127977015293 1105 58-59 0.17885152868507959 1105 60-61 0.07099159803866684 1105 62-63 0.17021815991842004 1105 64-65 0.0640734473547866 1105 66-67 0.06669392405858332 1105 68-69 0.1507313780849735 1105 70-71 0.12924399686652066 1105 72-73 0.17595902843257605 1105 74-75 0.1711655903861633 1105 76-77 0.2947844514106954 1105 78-79 0.20670019565017483 1105 80-81 0.13653642561638435 1105 82-83 0.16988879440558335 1105 84-85 0.1391277152523358 1105 86-87 0.184344665778017 1105 88-89 0.20835147113757913 1105 90-91 0.2841592573170466 1105 92-93 0.18994514314690392 1105 94-95 0.23166591338435794 1105 96-97 0.14299477800531335 1105 98-99 0.15735811378825204 1105 100-101 0.22781932676331706 1106 1 -0.10902585950181987 1106 2 -0.1507589258139248 1106 3 -0.15526691850642038 1106 4 -0.30403155714455465 1106 5 -0.1240250347200984 1106 6 -0.07745749301988525 1106 7 -0.09569278255736435 1106 8 -0.06069096229978754 1106 9 -0.104962051309343 1106 10-11 -0.11086236305960284 1106 12-13 -0.11251275348042356 1106 14-15 -0.2539622023324597 1106 16-17 -0.16012332763083492 1106 18-19 -0.2218328101509286 1106 20-21 -0.3223731665444163 1106 22-23 -0.16179115725383753 1106 24-25 -0.07999384562914713 1106 26-27 -0.3748806604702182 1106 28-29 -0.23107540677721516 1106 30-31 -0.2226592028351675 1106 32-33 -0.2680701683072684 1106 34-35 -0.16676195669333538 1106 36-37 -0.36663065969302266 1106 38-39 -0.23228028714657256 1106 40-41 -0.29546032296492797 1106 42-43 -0.2723991013497553 1106 44-45 -0.27481258450639956 1106 46-47 -0.19170008639589042 1106 48-49 -0.1343531130878901 1106 50-51 -0.3058166435632046 1106 52-53 -0.30878829252979756 1106 54-55 -0.2620473178871805 1106 56-57 -0.13049000232875585 1106 58-59 -0.10841818086367283 1106 60-61 -0.22786565997674302 1106 62-63 -0.0942622137673581 1106 64-65 -0.1460181225784254 1106 66-67 -0.16758806590849673 1106 68-69 -0.06943062157600721 1106 70-71 -0.0379385078676755 1106 72-73 -0.07986632178207742 1106 74-75 -0.02090454121849561 1106 76-77 0.06850487642968162 1106 78-79 -0.10302236785243224 1106 80-81 -0.16395596164030835 1106 82-83 -0.07502294766645079 1106 84-85 -0.14649622893510283 1106 86-87 -0.09418809008368711 1106 88-89 -0.02889269659414495 1106 90-91 -0.0262460442313035 1106 92-93 -0.15491939472417826 1106 94-95 -0.11214230647876988 1106 96-97 -0.25948421458027227 1106 98-99 -0.15476666785151494 1106 100-101 -0.1254085114800354 1107 1 -0.23844351152184018 1107 2 -0.31627210499035385 1107 3 -0.31423536179643463 1107 4 -0.20819438109991495 1107 5 -0.2869235807553636 1107 6 -0.18255580838603436 1107 7 -0.22590624728155007 1107 8 -0.2315465028927406 1107 9 -0.19481289975701088 1107 10-11 -0.2390625266311659 1107 12-13 -0.1648249495244798 1107 14-15 -0.22245976216997576 1107 16-17 -0.2907065271025644 1107 18-19 -0.31901460661971726 1107 20-21 -0.3348801839007933 1107 22-23 -0.2864014443811129 1107 24-25 -0.3454487103931001 1107 26-27 -0.25196588074474136 1107 28-29 -0.30062082513427413 1107 30-31 -0.1903436604285318 1107 32-33 -0.2554044712058783 1107 34-35 -0.4118186511427595 1107 36-37 -0.32534258257852144 1107 38-39 -0.3377954928733615 1107 40-41 -0.34173435259428686 1107 42-43 -0.3319483003301755 1107 44-45 -0.3143198858352818 1107 46-47 -0.2600938025570869 1107 48-49 -0.30825072716543644 1107 50-51 -0.32585361107751254 1107 52-53 -0.18104920748323394 1107 54-55 -0.20865592842719138 1107 56-57 -0.19429820977257606 1107 58-59 -0.21410055145075546 1107 60-61 -0.2345016161743061 1107 62-63 -0.20786880073723069 1107 64-65 -0.3110199468994992 1107 66-67 -0.23005372631059373 1107 68-69 -0.18648792995098162 1107 70-71 -0.0951486973288489 1107 72-73 -0.08662547606343907 1107 74-75 -0.1285500856389028 1107 76-77 -0.39102725014116047 1107 78-79 -0.4272529194615622 1107 80-81 -0.3011760028610233 1107 82-83 -0.23207244786208392 1107 84-85 -0.30649656101688194 1107 86-87 -0.33242404611094756 1107 88-89 -0.2616515426354944 1107 90-91 -0.17986220196845082 1107 92-93 -0.2783284228357559 1107 94-95 -0.2666505040027154 1107 96-97 -0.26908129294178096 1107 98-99 -0.30431541128385575 1107 100-101 -0.3931764918991334 1108 1 -0.4524942061045607 1108 2 -0.40780150415868377 1108 3 -0.4066860569953832 1108 4 -0.4863096314936257 1108 5 -0.6974346591465945 1108 6 -0.4468899013460046 1108 7 -0.4677601316799027 1108 8 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0.13945137041805822 2305 20-21 0.17311903399167505 2305 22-23 0.1838637476933016 2305 24-25 0.16862101444262123 2305 26-27 0.1424453304010811 2305 28-29 0.13628531619156092 2305 30-31 0.0890976929005447 2305 32-33 0.19664045187459323 2305 34-35 0.13852289455832079 2305 36-37 0.03833083597145048 2305 38-39 0.1389171324389622 2305 40-41 0.09178402981796552 2305 42-43 0.1273868556180986 2305 44-45 0.11441121841016155 2305 46-47 0.07707878689910075 2305 48-49 0.0444840642064932 2305 50-51 0.07908697625541805 2305 52-53 0.03714811511266447 2305 54-55 0.06801567204288972 2305 56-57 0.12171280805350904 2305 58-59 0.07805545676136916 2305 60-61 0.22593135161248767 2305 62-63 0.20868973111113576 2305 64-65 0.12371695719051701 2305 66-67 0.1955298866833246 2305 68-69 0.08956726212385391 2305 70-71 0.17431129760565156 2305 72-73 0.11726969869787496 2305 74-75 0.20198773417570592 2305 76-77 0.27214958783364906 2305 78-79 0.22155338386684775 2305 80-81 0.20748121985441514 2305 82-83 0.12553171558844767 2305 84-85 0.1001234383841485 2305 86-87 0.09266000412269548 2305 88-89 0.10360194457297567 2305 90-91 0.13773052724522827 2305 92-93 0.1717218522560806 2305 94-95 0.2672322032872678 2305 96-97 0.297188660266972 2305 98-99 0.2634784625244784 2305 100-101 0.4026610507870778 2306 1 -0.2718574015472761 2306 2 -0.4919955303384249 2306 3 -0.34155118812583396 2306 4 -0.2169119698815436 2306 5 -0.2315768718898923 2306 6 -0.26463199425698036 2306 7 -0.13536940931297892 2306 8 -0.12846806584404646 2306 9 -0.16419379353279595 2306 10-11 -0.13921735979010208 2306 12-13 -0.1653429306449894 2306 14-15 -0.25332464622299966 2306 16-17 -0.21365889738896016 2306 18-19 -0.15662287985963985 2306 20-21 -0.13550833041494315 2306 22-23 -0.19571175839777055 2306 24-25 -0.24116913174500354 2306 26-27 -0.18517229288169545 2306 28-29 -0.1616723572127441 2306 30-31 -0.16361851758710344 2306 32-33 -0.16714631189947937 2306 34-35 -0.12294697716892955 2306 36-37 -0.34023128917016976 2306 38-39 -0.29912494109223076 2306 40-41 -0.21009392001884208 2306 42-43 -0.15935678558835775 2306 44-45 -0.08338361745349232 2306 46-47 -0.20194770484108915 2306 48-49 -0.24579574263419346 2306 50-51 -0.4233001213477081 2306 52-53 -0.1307078597199336 2306 54-55 -0.18166192186449592 2306 56-57 -0.1645292991517735 2306 58-59 -0.12340476044155935 2306 60-61 -0.08620822156969155 2306 62-63 -0.05974220271639297 2306 64-65 -0.05140644759570989 2306 66-67 -0.10647781860241423 2306 68-69 -0.09114388546434427 2306 70-71 -0.3633836049771908 2306 72-73 -0.3230828144662361 2306 74-75 -0.2399984241823958 2306 76-77 -0.13836832135891441 2306 78-79 -0.13158300405862278 2306 80-81 -0.1161519668486477 2306 82-83 -0.02143941328418819 2306 84-85 -0.12799196890538767 2306 86-87 -0.21404491834529082 2306 88-89 -0.29922100246604444 2306 90-91 -0.2515927382565586 2306 92-93 -0.1435390704860069 2306 94-95 -0.03820596755611305 2306 96-97 -0.03998253626466308 2306 98-99 -0.08490719448508699 2306 100-101 -0.0017715152114341493 2307 1 -0.4076248090064709 2307 2 -0.33110014605735927 2307 3 -0.506697731495823 2307 4 -0.26605842751160225 2307 5 -0.371805595880879 2307 6 -0.26469570225843597 2307 7 -0.20344930076044676 2307 8 -0.22172352975177034 2307 9 -0.2474664116194205 2307 10-11 -0.43462618270405784 2307 12-13 -0.2817772588482228 2307 14-15 -0.4697650144119976 2307 16-17 -0.5531287309716149 2307 18-19 -0.5808425991969415 2307 20-21 -0.6782706933936211 2307 22-23 -0.6562377565196087 2307 24-25 -0.4583538348539804 2307 26-27 -0.3347255601544603 2307 28-29 -0.34739763357779196 2307 30-31 -0.2494109967502709 2307 32-33 -0.27974555191065775 2307 34-35 -0.2056706008039484 2307 36-37 -0.34852230332283085 2307 38-39 -0.31878139268815175 2307 40-41 -0.2747766420262465 2307 42-43 -0.43501923319909963 2307 44-45 -0.44751035971672337 2307 46-47 -0.25465257338272096 2307 48-49 -0.40169972206789595 2307 50-51 -0.24722830256227724 2307 52-53 -0.26477085751127305 2307 54-55 -0.3595543508016732 2307 56-57 -0.19601779988590096 2307 58-59 -0.21159860187182034 2307 60-61 -0.2518458161270374 2307 62-63 -0.11783297783632207 2307 64-65 -0.1417286872878094 2307 66-67 -0.2649545584468953 2307 68-69 -0.26453148881244104 2307 70-71 -0.2548885163750221 2307 72-73 -0.2281317127184721 2307 74-75 -0.24593548075265304 2307 76-77 -0.5757866370454856 2307 78-79 -0.5195182718228395 2307 80-81 -0.3952100743330753 2307 82-83 -0.27589237860238924 2307 84-85 -0.21829619529898636 2307 86-87 -0.277247114429759 2307 88-89 -0.28110082408983317 2307 90-91 -0.2965601467616992 2307 92-93 -0.2705391736698104 2307 94-95 -0.20868337725239527 2307 96-97 -0.24082623247722168 2307 98-99 -0.3100198193180539 2307 100-101 -0.24439532752246507 2308 1 -0.5508900093086737 2308 2 -0.4625683149488644 2308 3 -0.41780655811338363 2308 4 -0.6924706322662288 2308 5 -0.4509431285500298 2308 6 -0.7746410525543794 2308 7 -1.3648186926027677 2308 8 -0.5555911147331898 2308 9 -0.6613447669723982 2308 10-11 -0.4124220952398403 2308 12-13 -0.3740183398678809 2308 14-15 -0.9364387784852184 2308 16-17 -1.188987901130922 2308 18-19 -0.6736848711488435 2308 20-21 -0.5452855527210474 2308 22-23 -0.5694894704864595 2308 24-25 -0.5533575533830373 2308 26-27 -0.5116576906285744 2308 28-29 -0.8618726227482014 2308 30-31 -1.3880558168909332 2308 32-33 -0.6753081349066505 2308 34-35 -0.9495558356336247 2308 36-37 -1.1986630924155506 2308 38-39 -0.8078020860420381 2308 40-41 -0.8453860556027522 2308 42-43 -0.6477646476667047 2308 44-45 -0.865359095279338 2308 46-47 -1.1153536444370857 2308 48-49 -1.8549907384148412 2308 50-51 -2.668475409113654 2308 52-53 -1.8751213325465343 2308 54-55 -1.4457770948597783 2308 56-57 -0.6942822841187137 2308 58-59 -0.8078126134677603 2308 60-61 -0.3360210904336327 2308 62-63 -0.3786285418688209 2308 64-65 -0.46396411986903274 2308 66-67 -0.5070106538099495 2308 68-69 -0.8888614726756181 2308 70-71 -1.0683959119401898 2308 72-73 -1.1337940072595316 2308 74-75 -1.1647885418409523 2308 76-77 -0.9430728092368632 2308 78-79 -0.6615987780812134 2308 80-81 -0.8118808636171018 2308 82-83 -0.7450074144070058 2308 84-85 -1.0315790836772756 2308 86-87 -0.9016109133193275 2308 88-89 -0.9521385506915685 2308 90-91 -1.2656991051841366 2308 92-93 -0.9472703143087955 2308 94-95 -1.6614321820771956 2308 96-97 -1.1393095246299332 2308 98-99 -1.5293707748916034 2308 100-101 -1.749669130261129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 199.0 11 1893.0 12 5732.0 13 11404.0 14 19251.0 15 30034.0 16 44179.0 17 73420.0 18 148656.0 19 350959.0 20 428409.0 21 356011.0 22 330384.0 23 299362.0 24 292230.0 25 288461.0 26 292554.0 27 295257.0 28 294398.0 29 298883.0 30 311257.0 31 338010.0 32 386894.0 33 474394.0 34 637424.0 35 944406.0 36 1561985.0 37 2779273.0 38 3425669.0 39 755227.0 40 519.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.173358592752283 37.901223998472545 20.395438702025974 16.529978706749198 2 17.991204217168544 24.462252535822316 42.62046226158166 14.92608098542748 3 17.67670635957778 35.53369622210369 22.426461395119837 24.36313602319869 4 26.140112885566623 22.01081817321289 34.817298351364165 17.031770589856325 5 18.302109585775035 30.352635746493046 21.407977845456422 29.9372768222755 6 18.092188048706657 21.982543475739554 33.53541299246902 26.389855483084773 7 26.109098592177226 21.82285863265088 21.578737375809563 30.489305399362333 8 38.34112944364558 21.909498353496392 21.56611843518675 18.18325376767128 9 17.61820564867202 34.30859286535565 21.945978916095676 26.127222569876658 10-11 34.46673668574153 21.755993754496668 21.836692299764778 21.94057725999702 12-13 27.872747041405542 21.6803869938309 22.15029561310826 28.296570351655298 14-15 27.76478049149255 21.61437796796288 22.166383930460785 28.454457610083782 16-17 27.84759511615337 25.5780078937838 22.087643162462882 24.486753827599944 18-19 23.885832509246942 21.630589397569967 26.180786839084604 28.30279125409849 20-21 23.714885600871245 31.955218591001266 22.40992366930105 21.919972138826438 22-23 17.217411075960502 25.380208596328895 32.92092398431981 24.481456343390782 24-25 17.213407737484683 21.667231492947316 36.801192241128376 24.318168528439625 26-27 17.21548203111911 21.655318769230888 43.349179274251036 17.780019925398964 28-29 17.197848900301 21.476099448624016 43.58317177363313 17.74287987744185 30-31 17.225593434915343 21.441582569783975 43.553532217687476 17.77929177761321 32-33 17.285567434682132 21.4637578442034 43.51607184321548 17.734602877898993 34-35 17.29013921006124 21.47903110027404 43.5150872417662 17.71574244789852 36-37 17.289410474550998 21.47247029395957 43.46255176079421 17.775567470695215 38-39 17.51866234792899 21.559248583065642 42.71833321201866 18.203755856986707 40-41 18.497266900678326 22.357729106279283 39.70526665798457 19.43973733505782 42-43 18.58653118524501 22.683904693256306 39.926932457106936 18.802631664391747 44-45 18.67932885294993 23.24885532526849 39.356184858899134 18.71563096288245 46-47 18.420732282145323 23.682379909541687 39.333348883770455 18.563538924542538 48-49 18.476151560507134 23.868049637378704 38.92610131879999 18.729697483314173 50-51 18.56203609488513 24.1519117062436 38.41366722682451 18.87238497204676 52-53 18.652313342178164 24.389849203897455 37.98789335623952 18.969944097684866 54-55 18.981519980311 24.49685691248261 37.23566730575801 19.285955801448388 56-57 19.1505540412755 24.65503544513749 36.600171160131644 19.594239353455364 58-59 19.34408186541875 24.882959681761985 35.95436706929725 19.818591383522012 60-61 19.556671702422555 25.082823118713556 35.23974201922289 20.120763159641 62-63 19.79602609982015 25.394167439062564 34.48317749189348 20.326628969223805 64-65 19.999305409054298 25.68664837900242 33.852362917630884 20.4616832943124 66-67 20.11856513571185 25.87425957365011 33.19565347319927 20.811521817438766 68-69 20.24580804010593 26.1740059457931 32.56212144160928 21.01806457249169 70-71 20.42504756216925 26.41793742778038 32.03554130247569 21.12147370757468 72-73 20.520176739507335 26.571421685339473 31.571458515762384 21.3369430593908 74-75 20.661365849434908 26.703308880780398 31.162334108500584 21.47299116128411 76-77 20.7716286872433 26.842646487098587 30.79233092819787 21.593393897460242 78-79 20.864480970231092 26.818741255169726 30.52357380778174 21.793203966817444 80-81 20.918287514663323 26.899403848055254 30.29901913109224 21.88328950618918 82-83 21.00647166061114 27.057436694634536 30.06011547539369 21.875976169360637 84-85 21.07816148353639 27.084569743962284 29.86186166239365 21.975407110107675 86-87 21.175656755106303 27.189177764724302 29.62302798687068 22.012137493298713 88-89 21.21032059557589 27.2815278937849 29.461697606848148 22.046453903791054 90-91 21.28417335065094 27.220877892555436 29.29156664152893 22.203382115264688 92-93 21.22972133526363 27.217069828815305 29.26188755327836 22.291321282642706 94-95 21.282609374090367 27.31502011116953 29.091610672362616 22.310759842377482 96-97 21.32235333769583 27.32006615989399 29.023272467066576 22.334308035343604 98-99 21.409079024535295 27.408840017648643 28.86829399191027 22.313786965905788 100-101 21.561087667849186 27.6575278812961 28.328367329051485 22.453017121803228 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4256.0 1 2561.5 2 660.0 3 411.0 4 347.5 5 324.0 6 338.5 7 363.0 8 374.0 9 388.5 10 407.5 11 441.5 12 660.0 13 1220.0 14 1848.0 15 3789.0 16 121259.0 17 150995.0 18 65395.0 19 66882.5 20 71221.5 21 79489.5 22 89207.0 23 98130.5 24 106389.5 25 117281.5 26 132601.0 27 151201.5 28 170608.0 29 197022.0 30 234937.5 31 273943.5 32 313881.5 33 365057.0 34 412029.0 35 457550.0 36 508071.5 37 543712.0 38 567784.5 39 590062.0 40 606426.5 41 600624.0 42 584322.0 43 577668.0 44 569480.5 45 562435.0 46 546657.0 47 525949.0 48 506586.5 49 480916.5 50 453421.0 51 427776.5 52 411955.0 53 393250.5 54 362412.5 55 324700.0 56 287374.0 57 253030.0 58 221871.5 59 189151.5 60 151707.5 61 120158.0 62 97015.0 63 74973.5 64 57456.0 65 44966.0 66 33276.5 67 23583.0 68 17573.5 69 13682.0 70 11067.5 71 9639.0 72 8740.5 73 7059.0 74 5270.0 75 4208.0 76 3235.0 77 2280.5 78 1493.5 79 955.0 80 538.5 81 340.5 82 235.5 83 145.0 84 79.5 85 45.5 86 19.5 87 2.0 88 1.5 89 1.0 90 1.5 91 1.5 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.6153277806979314E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.230655561395863E-6 24-25 0.0 26-27 3.8767866736750354E-5 28-29 0.0 30-31 0.002830054271782776 32-33 0.005792565421582782 34-35 0.0014957935249262846 36-37 0.0016347117140663066 38-39 0.0029302045941860476 40-41 0.002846207549589755 42-43 0.0023874544598715425 44-45 0.006787607334492708 46-47 0.0013827205802774292 48-49 3.327575228237739E-4 50-51 0.0028591301718353386 52-53 0.0017703992476449327 54-55 4.781370230865877E-4 56-57 0.0 58-59 1.0984228908745934E-4 60-61 5.039822675777546E-4 62-63 0.0 64-65 0.0 66-67 6.461311122791726E-5 68-69 0.005937944921845596 70-71 0.006057479177617243 72-73 0.0025780631379938987 74-75 0.0032629621170098214 76-77 0.03217732939150279 78-79 0.05600341415679728 80-81 0.04807538540913183 82-83 6.752070123317353E-4 84-85 9.562740461731754E-4 86-87 0.0014731789359965135 88-89 0.0035827970175880118 90-91 3.230655561395863E-6 92-93 6.461311122791726E-6 94-95 7.753573347350071E-5 96-97 0.005776412143775803 98-99 0.001250263702260199 100-101 4.199852229814622E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5476735E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.08306036141025 #Duplication Level Percentage of deduplicated Percentage of total 1 78.81812512462261 33.16907917192678 2 12.017245639399352 10.114449472414739 3 3.7307535957343463 4.710045862885105 4 1.7235996373086944 2.9013739030306636 5 0.8861854108475465 1.8646697068049223 6 0.5428256577392948 1.3706258952218975 7 0.36373308431057816 1.071490093973879 8 0.2520850840895585 0.8486809447961654 9 0.17965594399652918 0.6804424741942869 >10 1.1121389623408802 9.371622106940121 >50 0.1976167763359351 5.969795721477676 >100 0.1719308359577093 13.050779132065705 >500 0.0026435334630622496 0.6900098120657706 >1k 0.0011499276204269718 0.9824514968631693 >5k 1.3985380513540516E-4 0.45845243233233296 >10k+ 1.7093242849882854E-4 12.746031773006717 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 1691478 10.929165615357503 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 118020 0.7625639387118794 No Hit GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22092 0.1427432853247148 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 19132 0.1236178044012513 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18538 0.11977978559431302 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 16860 0.1089377055302685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.8767866736750354E-5 0.0 0.0 0.0 0.0 2 3.8767866736750354E-5 0.0 0.0 0.0 0.0 3 4.522917785954208E-5 0.0 0.0 6.461311122791726E-6 0.0 4 5.1690488982333806E-5 0.0 0.0 6.461311122791726E-6 0.0 5 5.1690488982333806E-5 0.0 0.0 6.461311122791726E-6 0.0 6 5.1690488982333806E-5 0.0 0.0 6.461311122791726E-6 0.0 7 5.815180010512553E-5 0.0 0.0 6.461311122791726E-6 0.0 8 5.815180010512553E-5 0.0 0.0 6.461311122791726E-6 0.0 9 5.815180010512553E-5 0.0 0.0 6.461311122791726E-6 0.0 10-11 5.815180010512553E-5 0.0 0.0 6.461311122791726E-6 0.0 12-13 6.461311122791726E-5 0.0 0.0 9.691966684187589E-6 0.0 14-15 6.784376678931312E-5 6.461311122791726E-6 0.0 1.9383933368375177E-5 0.0 16-17 7.107442235070899E-5 6.461311122791726E-6 0.0 2.5845244491166903E-5 0.0 18-19 7.753573347350071E-5 6.461311122791726E-6 0.0 3.8767866736750354E-5 0.0 20-21 8.722770015768831E-5 6.461311122791726E-6 0.0 4.522917785954208E-5 3.230655561395863E-6 22-23 1.130729446488552E-4 6.461311122791726E-6 0.0 5.1690488982333806E-5 6.461311122791726E-6 24-25 1.3245687801723038E-4 9.691966684187589E-6 0.0 5.815180010512553E-5 6.461311122791726E-6 26-27 1.389181891400221E-4 1.9383933368375177E-5 0.0 7.430507791210484E-5 6.461311122791726E-6 28-29 1.4537950026281382E-4 2.5845244491166903E-5 0.0 9.368901128048003E-5 6.461311122791726E-6 30-31 1.6153277806979315E-4 2.5845244491166903E-5 0.0 1.0338097796466761E-4 9.691966684187589E-6 32-33 1.8091671143816832E-4 2.9075900052562766E-5 0.0 1.0661163352606348E-4 1.9383933368375177E-5 34-35 2.0676195592933522E-4 3.553721117535449E-5 0.0 1.0984228908745934E-4 1.9383933368375177E-5 36-37 2.261458892977104E-4 3.8767866736750354E-5 0.0 1.1630360021025106E-4 1.9383933368375177E-5 38-39 2.4876047822748143E-4 3.8767866736750354E-5 0.0 1.2922622245583451E-4 1.9383933368375177E-5 40-41 2.842976894028359E-4 3.8767866736750354E-5 0.0 1.3245687801723038E-4 1.9383933368375177E-5 42-43 2.9722031164841937E-4 4.522917785954208E-5 0.0 1.4861015582420968E-4 1.9383933368375177E-5 44-45 3.0691227833260697E-4 4.522917785954208E-5 0.0 1.5830212250839728E-4 1.9383933368375177E-5 46-47 3.2629621170098216E-4 4.522917785954208E-5 0.0 1.6153277806979315E-4 1.9383933368375177E-5 48-49 3.553721117535449E-4 4.522917785954208E-5 0.0 1.6476343363118902E-4 1.9383933368375177E-5 50-51 3.8767866736750357E-4 4.522917785954208E-5 0.0 1.7768605587677245E-4 1.9383933368375177E-5 52-53 5.589034121214842E-4 4.522917785954208E-5 0.0 2.003006448065435E-4 1.9383933368375177E-5 54-55 7.785879902964029E-4 4.522917785954208E-5 0.0 2.0676195592933522E-4 1.9383933368375177E-5 56-57 0.0010758083019448222 4.522917785954208E-5 0.0 2.0676195592933522E-4 2.5845244491166903E-5 58-59 0.0015894825362067645 5.1690488982333806E-5 0.0 2.1322326705212695E-4 2.5845244491166903E-5 60-61 0.002258228237415708 5.815180010512553E-5 0.0 2.1322326705212695E-4 3.553721117535449E-5 62-63 0.003085276061133049 6.461311122791726E-5 0.0 2.196845781749187E-4 3.8767866736750354E-5 64-65 0.004225697474305789 6.461311122791726E-5 0.0 2.2291523373631455E-4 3.8767866736750354E-5 66-67 0.005550266254478093 7.107442235070899E-5 0.0 2.261458892977104E-4 4.199852229814622E-5 68-69 0.007065443712772752 7.107442235070899E-5 0.0 2.35837855981898E-4 4.845983342093794E-5 70-71 0.009359209161363815 7.107442235070899E-5 0.0 2.4552982266608556E-4 5.1690488982333806E-5 72-73 0.012360488177900572 7.107442235070899E-5 0.0 2.4552982266608556E-4 5.1690488982333806E-5 74-75 0.01588190273982206 7.107442235070899E-5 0.0 2.4552982266608556E-4 5.1690488982333806E-5 76-77 0.020220673158776705 7.107442235070899E-5 0.0 2.5522178935027316E-4 5.1690488982333806E-5 78-79 0.025674019746412924 7.753573347350071E-5 0.0 2.713750671572525E-4 5.1690488982333806E-5 80-81 0.03239055265855492 8.399704459629244E-5 0.0 2.713750671572525E-4 5.1690488982333806E-5 82-83 0.04070949072914927 8.399704459629244E-5 0.0 2.7460572271864836E-4 5.1690488982333806E-5 84-85 0.05112512425908953 8.722770015768831E-5 0.0 2.9722031164841937E-4 5.1690488982333806E-5 86-87 0.06389913634884878 1.0338097796466761E-4 0.0 3.1014293389400283E-4 5.1690488982333806E-5 88-89 0.07897983650944465 1.0338097796466761E-4 0.0 3.230655561395863E-4 5.1690488982333806E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 122295 0.0 94.2896 1 ATATCTC 203750 0.0 92.9009 1 TATCTCG 203800 0.0 92.79188 2 TCGACGG 126410 0.0 91.21269 2 ATCTCGA 207325 0.0 91.12486 3 CGACGGC 126985 0.0 90.68373 3 TCTCGAG 205605 0.0 90.24947 4 CTCGAGG 206080 0.0 90.08524 5 GACGGCG 127965 0.0 90.02636 4 ACGGCGC 128045 0.0 89.93302 5 TCGAGGG 206835 0.0 89.76788 6 CGAGGGC 207695 0.0 89.35274 7 CGGCGCG 129155 0.0 89.24836 6 AGGGCGC 208315 0.0 89.18711 9 GAGGGCG 210250 0.0 88.427284 8 CTCGACG 4275 0.0 78.09856 5 TCTCGAC 6185 0.0 54.441532 4 GGGCGCG 208865 0.0 44.5398 10-11 GGATCCT 212115 0.0 43.246117 18-19 GATCCTT 209560 0.0 43.17735 20-21 >>END_MODULE