FastQCFastQC Report
Tue 31 May 2016
SRR1051290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15476735
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1038700.6711363863243766No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT170190.10996505399879238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG117000.089.153971
TCGACGG119900.086.9992
CGACGGC127450.082.291463
ACGGCGC135400.077.458975
GACGGCG137700.076.165424
CGGCGCG148650.070.522676
CGCGCCG223950.050.013139
GGCGCGC233250.047.9986727
GCGCGCC237100.047.4196138
TATCTCG25050.041.338042
ATCTCGA67450.029.7318881
GCCGGAT222100.027.40699212-13
CGCCGGA217450.026.03803310-11
CCGGATC225350.025.19900112-13
CTCGAGG79200.025.129533
GCGCCGG247050.025.03326210-11
CGGATCC248800.024.59944714-15
TCGAGGG81350.024.5236134
TCTCGAG81800.024.2730372
TCCATAT222350.023.95815818-19
Produced by FastQC (version 0.11.5)