FastQCFastQC Report
Tue 31 May 2016
SRR1051289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17039080
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT11648786.836507604870685No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT925470.5431455219413255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG906700.093.932981
TATCTCG1395150.092.528042
TCGACGG925450.092.014472
ATATCTC1409600.091.650271
CGACGGC935500.090.939663
ATCTCGA1427450.090.424353
ACGGCGC940200.090.378985
GACGGCG943800.090.114764
CGGCGCG949950.089.5363546
TCTCGAG1437550.088.930064
CTCGAGG1438150.088.8896565
TCGAGGG1441900.088.6848456
AGGGCGC1454850.087.8791059
CGAGGGC1456800.087.676717
GAGGGCG1477600.086.548578
CTCGACG20250.059.8058935
GGGCGCG1456050.043.9946710-11
GATCCTT1493450.042.0296620-21
GGATCCT1516400.041.89454718-19
ATCCTTT1564750.040.63353720-21
Produced by FastQC (version 0.11.5)