FastQCFastQC Report
Tue 31 May 2016
SRR1051289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17039080
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA912010.5352460344103085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG94800.086.371841
TCGACGG97350.083.9733052
CGACGGC108300.075.614073
ACGGCGC113200.072.214725
GACGGCG120200.068.1674654
CGGCGCG125650.065.172756
CGCGCCG185100.048.218279
GGCGCGC192550.046.22937
GCGCGCC193800.046.0291638
TATCTCG26050.029.7218782
GCCGGAT187900.025.8733312-13
CGCCGGA182950.025.15837710-11
ATCTCGA57850.025.0548441
GCGCCGG204250.024.2091710-11
CCGGATC190850.024.12942112-13
CGGATCC217600.022.45105214-15
TCCATAT203000.020.50912518-19
TCGAGGG71650.020.4849454
TCTCGAG75000.019.9501272
CTCGAGG74500.019.8288673
Produced by FastQC (version 0.11.5)