FastQCFastQC Report
Tue 31 May 2016
SRR1051288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12428335
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT9251017.443482976601451No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT806610.6490088978129411No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA140220.11282283588268259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG879550.094.25751
TATCTCG1156350.092.54682
ATATCTC1161250.092.233991
TCGACGG908450.091.248482
CGACGGC914600.090.536243
ATCTCGA1182350.090.447423
GACGGCG922500.089.760914
ACGGCGC923200.089.631135
CTCGAGG1167950.089.614785
TCTCGAG1168150.089.583174
TCGAGGG1168850.089.570156
CGGCGCG930050.089.062886
AGGGCGC1179200.088.848439
CGAGGGC1179750.088.657
GAGGGCG1195200.087.714658
CTCGACG30400.075.145535
TCTCGAC44000.051.918734
GGGCGCG1184450.044.29747810-11
GGATCCT1222250.042.4592818-19
GATCCTT1206750.042.40642520-21
Produced by FastQC (version 0.11.5)