##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051288_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12428335 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.157600032506366 33.0 31.0 34.0 30.0 34.0 2 32.39925774450077 34.0 31.0 34.0 31.0 34.0 3 32.48034278123337 34.0 31.0 34.0 31.0 34.0 4 35.89532097420933 37.0 35.0 37.0 35.0 37.0 5 35.88189463834053 37.0 35.0 37.0 35.0 37.0 6 35.8902642228424 37.0 36.0 37.0 35.0 37.0 7 35.83014160786622 37.0 35.0 37.0 35.0 37.0 8 35.8151142530355 37.0 35.0 37.0 35.0 37.0 9 37.53669940502891 39.0 37.0 39.0 35.0 39.0 10-11 37.501217781786536 39.0 37.0 39.0 34.5 39.0 12-13 37.46452280212917 39.0 37.0 39.0 34.5 39.0 14-15 38.85210649696842 40.0 38.0 41.0 35.0 41.0 16-17 38.73827475683589 40.0 38.0 41.0 34.5 41.0 18-19 38.788670043091045 40.0 38.5 41.0 35.0 41.0 20-21 38.78253692067361 40.0 38.5 41.0 34.5 41.0 22-23 38.76361938264458 40.0 38.5 41.0 34.5 41.0 24-25 38.768678427158584 40.0 38.0 41.0 34.0 41.0 26-27 38.70639011581197 40.0 38.0 41.0 34.0 41.0 28-29 38.49991760762805 40.0 38.0 41.0 34.0 41.0 30-31 37.943481970835194 39.5 37.0 41.0 34.0 41.0 32-33 37.43222949815885 39.0 35.5 41.0 33.0 41.0 34-35 37.183387034546456 39.0 35.0 41.0 33.0 41.0 36-37 37.327425073430994 39.0 35.0 41.0 33.0 41.0 38-39 37.15225514922152 39.0 35.0 41.0 32.0 41.0 40-41 36.36602292261996 39.0 35.0 41.0 29.5 41.0 42-43 36.413635615711996 39.0 35.0 41.0 29.0 41.0 44-45 36.379338181663115 39.0 35.0 41.0 29.0 41.0 46-47 36.43081921271031 39.0 35.0 41.0 29.5 41.0 48-49 36.262088888012755 39.0 35.0 41.0 27.5 41.0 50-51 36.0397982111039 38.5 35.0 40.5 27.5 41.0 52-53 35.788024421614 39.0 35.0 40.5 27.5 41.0 54-55 35.42661072460631 39.0 35.0 41.0 26.0 41.0 56-57 34.935697380220276 39.0 34.5 41.0 24.5 41.0 58-59 34.502782150625976 38.0 34.0 41.0 22.5 41.0 60-61 34.24558088432602 38.0 34.0 41.0 20.5 41.0 62-63 33.89706372575249 38.0 34.0 40.5 18.0 41.0 64-65 33.52766372164896 37.5 34.0 40.0 16.5 41.0 66-67 33.04811718544761 37.0 33.5 40.0 9.0 41.0 68-69 32.58119820555207 36.0 33.0 39.0 7.0 41.0 70-71 32.087898539909006 36.0 32.5 39.0 3.5 41.0 72-73 31.660120885058213 35.0 32.0 39.0 2.0 40.5 74-75 31.22155767445921 35.0 32.0 37.5 2.0 40.0 76-77 30.729931241795462 35.0 31.5 37.0 2.0 39.0 78-79 30.364005355504176 35.0 31.0 36.5 2.0 39.0 80-81 30.020888598512997 35.0 31.0 36.0 2.0 38.0 82-83 29.68064571803061 35.0 31.0 36.0 2.0 37.0 84-85 29.328775616363735 35.0 30.5 35.0 2.0 37.0 86-87 29.02868529855367 35.0 30.0 35.0 2.0 36.5 88-89 28.733306271515858 35.0 30.0 35.0 2.0 36.0 90-91 28.51430955956691 35.0 30.0 35.0 2.0 36.0 92-93 28.314780982327882 34.0 29.0 35.0 2.0 35.5 94-95 28.03854120443326 34.0 29.0 35.0 2.0 35.0 96-97 27.755061760082906 34.0 29.0 35.0 2.0 35.0 98-99 27.455798383291082 34.0 29.0 35.0 2.0 35.0 100-101 26.37941759696693 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35050893409098194 1101 2 0.33313915625582524 1101 3 0.3726822367221203 1101 4 0.3386219593109274 1101 5 0.36882501207602303 1101 6 0.32750929684834773 1101 7 0.34704097638670106 1101 8 0.3049486653220441 1101 9 0.38441164195500477 1101 10-11 0.3187782376014354 1101 12-13 0.38699620197934337 1101 14-15 0.48533759249171027 1101 16-17 0.38658385373875603 1101 18-19 0.48231788267246145 1101 20-21 0.44116542573085127 1101 22-23 0.4408674822131218 1101 24-25 0.46537217379729867 1101 26-27 0.5271656519643173 1101 28-29 0.5852571814741836 1101 30-31 0.6757833936419573 1101 32-33 0.6067312807304859 1101 34-35 0.6457520186067214 1101 36-37 0.6774588563826001 1101 38-39 0.7140197679505604 1101 40-41 0.6885132924868245 1101 42-43 0.6451869938633195 1101 44-45 0.6898266590601807 1101 46-47 0.660592949902103 1101 48-49 0.66147330476295 1101 50-51 0.6280383698404677 1101 52-53 0.5696570236378093 1101 54-55 0.6398952813693981 1101 56-57 0.6242241321251285 1101 58-59 0.6268264996682191 1101 60-61 0.5514867051014392 1101 62-63 0.5872704401426248 1101 64-65 0.5138345708378225 1101 66-67 0.4948884194513923 1101 68-69 0.474945222519942 1101 70-71 0.5746563767039845 1101 72-73 0.5952020334669506 1101 74-75 0.5087459652562636 1101 76-77 0.40516067195659744 1101 78-79 0.3363147285137451 1101 80-81 0.32902487633574395 1101 82-83 0.3814623226512488 1101 84-85 0.4087587842092155 1101 86-87 0.4259771697412731 1101 88-89 0.45716507783123106 1101 90-91 0.4897896831883486 1101 92-93 0.49266453344613126 1101 94-95 0.5354893846579465 1101 96-97 0.5220298104243106 1101 98-99 0.5987625261236396 1101 100-101 0.7858519303569658 1102 1 0.32682903339340896 1102 2 0.2973521113526303 1102 3 0.3492921254316599 1102 4 0.24901502749167292 1102 5 0.23486254310802934 1102 6 0.25271259780456035 1102 7 0.2864405365109235 1102 8 0.23831509553188823 1102 9 0.30610655600931835 1102 10-11 0.31236661747097827 1102 12-13 0.3299139128790145 1102 14-15 0.47448394086200807 1102 16-17 0.4083999165201675 1102 18-19 0.4614875581460822 1102 20-21 0.3841680616817271 1102 22-23 0.3795144866213178 1102 24-25 0.432835584344879 1102 26-27 0.4670308692119747 1102 28-29 0.4828541771509407 1102 30-31 0.5052181686067954 1102 32-33 0.5189993961082067 1102 34-35 0.5596305536599075 1102 36-37 0.6171495333287709 1102 38-39 0.6393769900311881 1102 40-41 0.6067595533067305 1102 42-43 0.5767498802885243 1102 44-45 0.587387578551585 1102 46-47 0.5204430464199064 1102 48-49 0.5403654030459251 1102 50-51 0.5214022691126914 1102 52-53 0.4750735546131821 1102 54-55 0.509088814912289 1102 56-57 0.4410290859192969 1102 58-59 0.4382935349432344 1102 60-61 0.32667935724087727 1102 62-63 0.38567016781609453 1102 64-65 0.29207779925228294 1102 66-67 0.2965428798012155 1102 68-69 0.3158636106961552 1102 70-71 0.4010361801757938 1102 72-73 0.4313793088329625 1102 74-75 0.41695809086628444 1102 76-77 0.2975548504461081 1102 78-79 0.20996035241051558 1102 80-81 0.29794805972430183 1102 82-83 0.3559538342758657 1102 84-85 0.29381188670215863 1102 86-87 0.3357117221723911 1102 88-89 0.43041238878375765 1102 90-91 0.4543730755178963 1102 92-93 0.37576577567043046 1102 94-95 0.42759471767977786 1102 96-97 0.4481700597659106 1102 98-99 0.5421088740219595 1102 100-101 0.6051831020689882 1103 1 0.23531609120343688 1103 2 0.22456973400028346 1103 3 0.269498742899664 1103 4 0.21743978727165114 1103 5 0.25981983328108527 1103 6 0.23893333398849137 1103 7 0.28363897917646597 1103 8 0.2368438060416409 1103 9 0.31829516361636934 1103 10-11 0.3113959835614182 1103 12-13 0.3065604011469176 1103 14-15 0.4216779574815561 1103 16-17 0.44115153605523005 1103 18-19 0.44094421228992786 1103 20-21 0.3819708576685841 1103 22-23 0.40103241628757047 1103 24-25 0.40925948766979303 1103 26-27 0.40100551009211216 1103 28-29 0.3894117182433874 1103 30-31 0.44955394129936366 1103 32-33 0.40059258321999636 1103 34-35 0.47059899342374933 1103 36-37 0.38639971950586016 1103 38-39 0.34662358693105944 1103 40-41 0.3830459618330053 1103 42-43 0.35764952739815925 1103 44-45 0.3592015240617954 1103 46-47 0.3473764633166283 1103 48-49 0.3624412968240591 1103 50-51 0.29382665165062605 1103 52-53 0.33330138956137745 1103 54-55 0.3349814882184461 1103 56-57 0.3364837389375168 1103 58-59 0.36382636953006653 1103 60-61 0.3057337763359982 1103 62-63 0.38622951822760143 1103 64-65 0.3863200300077807 1103 66-67 0.30401570731715566 1103 68-69 0.3608075551438503 1103 70-71 0.42541307599321243 1103 72-73 0.4198802382351978 1103 74-75 0.36727030239798 1103 76-77 0.4431762285381673 1103 78-79 0.32448642721493925 1103 80-81 0.33610726584603867 1103 82-83 0.3938348551479258 1103 84-85 0.34372251630501083 1103 86-87 0.3847307411583394 1103 88-89 0.44278406210286647 1103 90-91 0.477366262272362 1103 92-93 0.37871013113700513 1103 94-95 0.3994954118375027 1103 96-97 0.40216623910727023 1103 98-99 0.4739321809734065 1103 100-101 0.5856835926420807 1104 1 0.28347079715906176 1104 2 0.24398528151728271 1104 3 0.17623561881561045 1104 4 0.16458607625155963 1104 5 0.18357787354731414 1104 6 0.2070460658504416 1104 7 0.21926247301136215 1104 8 0.17336529487618435 1104 9 0.23160900353953906 1104 10-11 0.20914841606512624 1104 12-13 0.2723993082981835 1104 14-15 0.32869826776848754 1104 16-17 0.37244344418770226 1104 18-19 0.32870626245355794 1104 20-21 0.3578181454608256 1104 22-23 0.3780026315387204 1104 24-25 0.32658935894505703 1104 26-27 0.34480951417024386 1104 28-29 0.3310197214653172 1104 30-31 0.38788477889095674 1104 32-33 0.38570427232402693 1104 34-35 0.4370478870780019 1104 36-37 0.40476115364826626 1104 38-39 0.35222751699943444 1104 40-41 0.28424548227840774 1104 42-43 0.28603071394729085 1104 44-45 0.32339207060960007 1104 46-47 0.28756443415591804 1104 48-49 0.29079533852012673 1104 50-51 0.2042769648742393 1104 52-53 0.22048017838374534 1104 54-55 0.3144966249857575 1104 56-57 0.3101876792932572 1104 58-59 0.3771504611359049 1104 60-61 0.28206409400650756 1104 62-63 0.41318257574990724 1104 64-65 0.36551077257011855 1104 66-67 0.4774604879420892 1104 68-69 0.4981493269279582 1104 70-71 0.5399948786709459 1104 72-73 0.5637779792655202 1104 74-75 0.5402133174104122 1104 76-77 0.5997959128740185 1104 78-79 0.4340955716438337 1104 80-81 0.4468032774137214 1104 82-83 0.44861459251073654 1104 84-85 0.43200368861114313 1104 86-87 0.442048679878706 1104 88-89 0.4767501751992853 1104 90-91 0.5746217620705565 1104 92-93 0.45008257303854293 1104 94-95 0.5750937186878495 1104 96-97 0.5895820213319674 1104 98-99 0.6006673684048991 1104 100-101 0.6549926408525799 1105 1 0.07054718764352685 1105 2 0.07341208413787825 1105 3 0.09190510155552545 1105 4 -0.022872596681118296 1105 5 0.10130476376136244 1105 6 0.11910473067152338 1105 7 0.13826357447583604 1105 8 0.13229843980189315 1105 9 0.1495205827377788 1105 10-11 0.06597818061515426 1105 12-13 0.1304806701882768 1105 14-15 0.21421781219199687 1105 16-17 0.10632732456662808 1105 18-19 0.18857333880516336 1105 20-21 0.09435620244165221 1105 22-23 0.1082381415564484 1105 24-25 0.12342648656707667 1105 26-27 0.16803996691654532 1105 28-29 0.21431821134954276 1105 30-31 0.22585553584089268 1105 32-33 0.13579958544944049 1105 34-35 0.23757948962859388 1105 36-37 0.23033332895644776 1105 38-39 0.23083223460752578 1105 40-41 0.12583217919326017 1105 42-43 0.08183977770346473 1105 44-45 0.06401051254482581 1105 46-47 0.10225400887683378 1105 48-49 0.12322278339915727 1105 50-51 0.06167815596006676 1105 52-53 0.005614150559587472 1105 54-55 0.10506924106677218 1105 56-57 0.18278965421640692 1105 58-59 0.13462119145734874 1105 60-61 0.06803242749340654 1105 62-63 0.18121443597171094 1105 64-65 0.05700167399510292 1105 66-67 0.11330999057511093 1105 68-69 0.16502476080986384 1105 70-71 0.16488035095103015 1105 72-73 0.2062479741042189 1105 74-75 0.16587728224545017 1105 76-77 0.3132225626884484 1105 78-79 0.26626632049201504 1105 80-81 0.1775194383901635 1105 82-83 0.18754681995955735 1105 84-85 0.17465507525145796 1105 86-87 0.22258618327248314 1105 88-89 0.2615301355449873 1105 90-91 0.3320945658208707 1105 92-93 0.2701604110347624 1105 94-95 0.31535943776430386 1105 96-97 0.23776075033410038 1105 98-99 0.23255675111030172 1105 100-101 0.2962314650094662 1106 1 -0.10914312703381057 1106 2 -0.15302988654744354 1106 3 -0.14599015541080007 1106 4 -0.30104945283638074 1106 5 -0.12201211136148515 1106 6 -0.07228206647264557 1106 7 -0.06591775111969866 1106 8 -0.08478508162296805 1106 9 -0.10665770470244951 1106 10-11 -0.1319925995643203 1106 12-13 -0.13047863823942407 1106 14-15 -0.2763255938192799 1106 16-17 -0.18707720454993648 1106 18-19 -0.2669547697605381 1106 20-21 -0.34159476291619484 1106 22-23 -0.18522020780988413 1106 24-25 -0.11161161455267887 1106 26-27 -0.4067736322074538 1106 28-29 -0.25962731416395 1106 30-31 -0.2350410989606857 1106 32-33 -0.2786143122034872 1106 34-35 -0.17228570321143621 1106 36-37 -0.3769234269756936 1106 38-39 -0.2140123998301604 1106 40-41 -0.22343454183852174 1106 42-43 -0.23371005148861457 1106 44-45 -0.2560829779116105 1106 46-47 -0.1036541935634574 1106 48-49 -0.031023111136974535 1106 50-51 -0.20438713963550015 1106 52-53 -0.20605709419771756 1106 54-55 -0.15242109855070396 1106 56-57 -0.07494128589689097 1106 58-59 -0.04145659854577133 1106 60-61 -0.04302603898000257 1106 62-63 0.07942371168066842 1106 64-65 0.009347773807306226 1106 66-67 -0.022024806742741987 1106 68-69 0.050362226773501106 1106 70-71 0.12230826268817196 1106 72-73 0.04005622655224528 1106 74-75 0.09625868786503489 1106 76-77 0.17021491534030275 1106 78-79 0.008913698355932809 1106 80-81 -0.027556457588651995 1106 82-83 0.05240868263683396 1106 84-85 0.004656448816877656 1106 86-87 0.023989784207170572 1106 88-89 0.08608660008865598 1106 90-91 0.12755435344520194 1106 92-93 0.026956466934500156 1106 94-95 0.0528509017455967 1106 96-97 -0.08536475946364064 1106 98-99 0.01006206463363668 1106 100-101 0.05318495949091684 1107 1 -0.2474938123024053 1107 2 -0.29869022101160425 1107 3 -0.313773054439757 1107 4 -0.2015775382616738 1107 5 -0.2656954926806705 1107 6 -0.16633822322459224 1107 7 -0.19640270645090396 1107 8 -0.21202673000392025 1107 9 -0.19144975236435613 1107 10-11 -0.28224380901700385 1107 12-13 -0.1742075762273103 1107 14-15 -0.22571837294844954 1107 16-17 -0.2710418762776001 1107 18-19 -0.2853692288186167 1107 20-21 -0.2948352576566222 1107 22-23 -0.25875994486360554 1107 24-25 -0.3050451683155515 1107 26-27 -0.23260905262790033 1107 28-29 -0.27894408723285125 1107 30-31 -0.1747532482558114 1107 32-33 -0.2426413081219394 1107 34-35 -0.36064963162450425 1107 36-37 -0.29881894383338903 1107 38-39 -0.31646570835233234 1107 40-41 -0.3642869699378295 1107 42-43 -0.35128442055983555 1107 44-45 -0.33677036218455925 1107 46-47 -0.30093985018766745 1107 48-49 -0.2922476332061734 1107 50-51 -0.3177306469143488 1107 52-53 -0.19091634229533128 1107 54-55 -0.15978250739227917 1107 56-57 -0.17032404918702326 1107 58-59 -0.19364927161139178 1107 60-61 -0.21870706484580893 1107 62-63 -0.20929831154082734 1107 64-65 -0.3386885110650013 1107 66-67 -0.24059688507164623 1107 68-69 -0.15078180129419394 1107 70-71 -0.06240697278975205 1107 72-73 -0.06984349684043778 1107 74-75 -0.11607095936604495 1107 76-77 -0.44872064321931404 1107 78-79 -0.44118382112399246 1107 80-81 -0.2792290657979706 1107 82-83 -0.17117840006983087 1107 84-85 -0.30318278119787223 1107 86-87 -0.3095985841094233 1107 88-89 -0.2385083996607129 1107 90-91 -0.1581601702343285 1107 92-93 -0.30313369054171346 1107 94-95 -0.28462393214609705 1107 96-97 -0.2900418446734605 1107 98-99 -0.31478151041220315 1107 100-101 -0.43924135809784204 1108 1 -0.4851216846296218 1108 2 -0.4016287259633273 1108 3 -0.4144482058494532 1108 4 -0.5023989307675407 1108 5 -0.7015355995680537 1108 6 -0.4358388176835746 1108 7 -0.4473722380837728 1108 8 -1.2193617456157781 1108 9 -0.7619600298349383 1108 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0.1696098159416053 2305 20-21 0.21039211716129103 2305 22-23 0.20883319792797295 2305 24-25 0.1632921687256399 2305 26-27 0.15427317761034232 2305 28-29 0.14162904571548296 2305 30-31 0.08007659777766918 2305 32-33 0.19755965954758636 2305 34-35 0.1702218646037963 2305 36-37 0.12715796854925543 2305 38-39 0.19262113560551342 2305 40-41 0.10552265056819721 2305 42-43 0.11102802144090163 2305 44-45 0.14369861729199584 2305 46-47 0.09005523553677364 2305 48-49 0.12532081459889355 2305 50-51 0.1310344098614067 2305 52-53 0.0806584909828274 2305 54-55 -0.0032789689796999255 2305 56-57 0.1291499525667419 2305 58-59 0.1066413764603098 2305 60-61 0.1560284697614236 2305 62-63 0.1683920761427018 2305 64-65 0.09209149397729277 2305 66-67 0.1408500658958829 2305 68-69 0.0660359021686645 2305 70-71 0.1281912129375229 2305 72-73 0.0410656971856973 2305 74-75 0.15029948707829632 2305 76-77 0.197227790490885 2305 78-79 0.18321636348702697 2305 80-81 0.1814802922470733 2305 82-83 0.13573418151268157 2305 84-85 0.14460975640725593 2305 86-87 0.10784262594666316 2305 88-89 0.12985955084168666 2305 90-91 0.15250839113510395 2305 92-93 0.15645463256132075 2305 94-95 0.21481372076246785 2305 96-97 0.3083106051611466 2305 98-99 0.288632974195572 2305 100-101 0.3984266449645908 2306 1 -0.24929956760231775 2306 2 -0.46558942792725944 2306 3 -0.3023692353380696 2306 4 -0.202354375244731 2306 5 -0.16930746609887137 2306 6 -0.21178507393618418 2306 7 -0.10079934700824111 2306 8 -0.09141413094054229 2306 9 -0.11714346990822833 2306 10-11 -0.11422260613817059 2306 12-13 -0.12409874753206651 2306 14-15 -0.21335479216509157 2306 16-17 -0.15168239327592659 2306 18-19 -0.09488686148608139 2306 20-21 -0.09656006236950532 2306 22-23 -0.14517378550547733 2306 24-25 -0.1836912535791484 2306 26-27 -0.11060671900023067 2306 28-29 -0.08158107650775293 2306 30-31 -0.13859964124977608 2306 32-33 -0.10787910121734967 2306 34-35 -0.07094730263842308 2306 36-37 -0.27953844383169724 2306 38-39 -0.24509417485128182 2306 40-41 -0.17835566251235235 2306 42-43 -0.15120465015964868 2306 44-45 -0.052416359865866013 2306 46-47 -0.17061046466663043 2306 48-49 -0.21767616252175515 2306 50-51 -0.397888427908768 2306 52-53 -0.1335503765967232 2306 54-55 -0.20532595925952535 2306 56-57 -0.16994042147461386 2306 58-59 -0.10281930880359624 2306 60-61 -0.052795931307763055 2306 62-63 -0.07915624018527012 2306 64-65 -0.08950586596958487 2306 66-67 -0.13027680938574093 2306 68-69 -0.11427121760629433 2306 70-71 -0.4084141503962968 2306 72-73 -0.3022035399863121 2306 74-75 -0.1740786818072415 2306 76-77 -0.1401657726363048 2306 78-79 -0.15550074998599683 2306 80-81 -0.12356805173100582 2306 82-83 0.001206110700035623 2306 84-85 -0.1019856454541106 2306 86-87 -0.1537162344638574 2306 88-89 -0.31574285174338 2306 90-91 -0.2793142765361303 2306 92-93 -0.13299953727772618 2306 94-95 -0.036729800192219386 2306 96-97 -0.033439816113745024 2306 98-99 -0.032446678688152986 2306 100-101 0.06292520431492576 2307 1 -0.3777154334727868 2307 2 -0.3182594733864761 2307 3 -0.5261245506823755 2307 4 -0.2553056312543234 2307 5 -0.3712600591502664 2307 6 -0.2732770611077058 2307 7 -0.2459435726401793 2307 8 -0.25394042550929186 2307 9 -0.2993522742555115 2307 10-11 -0.43653203212240754 2307 12-13 -0.2658210733656219 2307 14-15 -0.48645847176874923 2307 16-17 -0.5630351432249796 2307 18-19 -0.5834769717151502 2307 20-21 -0.7106490420201297 2307 22-23 -0.6617880312908895 2307 24-25 -0.48866676427051203 2307 26-27 -0.3063887044094642 2307 28-29 -0.31884138901086345 2307 30-31 -0.20687712981663964 2307 32-33 -0.30004139384282524 2307 34-35 -0.202156288236516 2307 36-37 -0.34442353827471095 2307 38-39 -0.3416516817824018 2307 40-41 -0.3041783954431949 2307 42-43 -0.4590689135472559 2307 44-45 -0.47882743312536746 2307 46-47 -0.2627323977952827 2307 48-49 -0.3730366551574136 2307 50-51 -0.2849173815992643 2307 52-53 -0.28805722245163423 2307 54-55 -0.36687951656935525 2307 56-57 -0.2199062175679174 2307 58-59 -0.24315745778606157 2307 60-61 -0.3232841050679056 2307 62-63 -0.1474726593651141 2307 64-65 -0.23156581867352344 2307 66-67 -0.329082134369834 2307 68-69 -0.36510863671904303 2307 70-71 -0.300244953621597 2307 72-73 -0.340567349444445 2307 74-75 -0.36179192330476084 2307 76-77 -0.6346032068351697 2307 78-79 -0.6045895608626566 2307 80-81 -0.5267271870409189 2307 82-83 -0.39461832187777546 2307 84-85 -0.33970585923169594 2307 86-87 -0.4282819301418357 2307 88-89 -0.449718665088902 2307 90-91 -0.449162223623528 2307 92-93 -0.4214314503937189 2307 94-95 -0.259178354679495 2307 96-97 -0.29675498010197643 2307 98-99 -0.36812158504449144 2307 100-101 -0.31658998446133424 2308 1 -0.5067803550078125 2308 2 -0.4847552765893006 2308 3 -0.4112643247401735 2308 4 -0.6905094925320014 2308 5 -0.43159188038121243 2308 6 -0.7208852040009006 2308 7 -1.374807830013701 2308 8 -0.5435562857306309 2308 9 -0.6190920990663287 2308 10-11 -0.39869518507294543 2308 12-13 -0.35089677511569306 2308 14-15 -0.9222298275046725 2308 16-17 -1.1750955966660186 2308 18-19 -0.6548802976653789 2308 20-21 -0.5551103002084901 2308 22-23 -0.5455922454716529 2308 24-25 -0.5727850721629153 2308 26-27 -0.53735368057567 2308 28-29 -0.8520354157327219 2308 30-31 -1.3304218831252612 2308 32-33 -0.6363622927856554 2308 34-35 -0.9270587579010225 2308 36-37 -1.1488235207559114 2308 38-39 -0.7319130271531833 2308 40-41 -0.8155309482916735 2308 42-43 -0.6016107827507966 2308 44-45 -0.8058745658372146 2308 46-47 -1.0235916342116624 2308 48-49 -1.7004286538054174 2308 50-51 -2.6339954775503855 2308 52-53 -1.7676125216059475 2308 54-55 -1.308335745275798 2308 56-57 -0.5782882712704023 2308 58-59 -0.7018102082215805 2308 60-61 -0.20982595653096325 2308 62-63 -0.19972018421591997 2308 64-65 -0.2349530418234167 2308 66-67 -0.2923274561437026 2308 68-69 -0.7084570076675902 2308 70-71 -0.8492917168997387 2308 72-73 -0.9433877928010865 2308 74-75 -0.9883678490900714 2308 76-77 -0.7669494851660765 2308 78-79 -0.47093329948468465 2308 80-81 -0.6289141422005109 2308 82-83 -0.554727115810767 2308 84-85 -0.8744463273031435 2308 86-87 -0.7280059724007764 2308 88-89 -0.8207514653862873 2308 90-91 -1.0869167954713213 2308 92-93 -0.7739911404418081 2308 94-95 -1.5923147121241534 2308 96-97 -0.9726777644650291 2308 98-99 -1.485555235004611 2308 100-101 -1.707605566713159 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 204.0 11 1438.0 12 3763.0 13 7484.0 14 12447.0 15 19009.0 16 28781.0 17 49281.0 18 104089.0 19 253583.0 20 311595.0 21 245375.0 22 212127.0 23 179141.0 24 167862.0 25 167665.0 26 176643.0 27 188552.0 28 197224.0 29 208636.0 30 225751.0 31 255238.0 32 302388.0 33 380590.0 34 520313.0 35 774627.0 36 1309352.0 37 2425521.0 38 3023833.0 39 675262.0 40 557.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 25.89230174436077 36.647764966103665 20.605672441240117 16.854260848295446 2 19.258187037925836 26.343062043306688 38.51289814766016 15.885852771107311 3 19.473227910255076 32.99385637738281 23.381860884824878 24.151054827537237 4 26.799856939807302 23.234029336994862 32.01942979490012 17.946683928297716 5 19.667702874117893 31.03874332322069 22.248000234947 27.04555356771442 6 19.390191847902393 23.433018179828593 30.321825087592185 26.85496488467683 7 26.418389913049495 23.04898443757752 22.785819661282062 27.746805988090923 8 34.64365902592745 23.285902737575064 22.604081721324697 19.46635651517279 9 18.743604835241406 31.717410256482463 23.03041396936919 26.50857093890694 10-11 31.28740897312472 23.120510510860868 23.019197664047518 22.572882851966895 12-13 26.595254038071392 22.96031390852547 23.41825139234269 27.026180661060444 14-15 26.544400356121717 22.81795590479336 23.45208348503641 27.185560254048514 16-17 26.654962229453904 26.36061467606079 23.313476825335012 23.670946269150292 18-19 23.146700664248268 22.85405889043062 26.872408894674948 27.126831550646163 20-21 23.04066071601707 30.848786261393823 23.462591730911665 22.647961291677447 22-23 18.463297778825563 26.244673964774844 31.606277107915098 23.685751148484492 24-25 18.460091395991498 22.827277346482855 35.09486186202738 23.61776939549827 26-27 18.457871096459282 22.8678997310339 39.690330126359676 18.983899046147144 28-29 18.458317224310417 22.718296537709996 39.871338357068744 18.95204788091084 30-31 18.5007810410144 22.68095068987363 39.8148667064506 19.003401562661367 32-33 18.543624994357312 22.698356857472515 39.78136467177986 18.97665347639031 34-35 18.54727788301372 22.73342115541177 39.78151468624715 18.937786275327365 36-37 18.574030966896817 22.703098057548573 39.72431850056042 18.998552474994188 38-39 18.750483286008517 22.785878207822197 39.06017899505638 19.403459511112903 40-41 19.61176037693689 23.588814069298046 36.35815313025 20.44127242351506 42-43 19.694362202129316 23.857430823386206 36.55686284985795 19.89134412462653 44-45 19.747990986435557 24.356998177910715 36.06904353670073 19.825967298953 46-47 19.5335726314569 24.757977885930984 36.04223846160871 19.666211021003406 48-49 19.586288268182315 24.923192379258243 35.627954172140235 19.862565180419207 50-51 19.653529974227958 25.164930982668455 35.18712562112259 19.994413421981 52-53 19.72277667798537 25.41954311128577 34.75994687202061 20.09773333870825 54-55 20.063675817688488 25.497531192203322 34.03460305725674 20.40418993285145 56-57 20.243411526966405 25.623738014786372 33.41773455575506 20.71511590249217 58-59 20.39543214464827 25.82323429372272 32.87759889162878 20.903734670000233 60-61 20.585605853234327 25.942858195755903 32.279293166533016 21.19224278447675 62-63 20.791075393445702 26.17852270638022 31.67140248472543 21.35899941544865 64-65 20.94861946305034 26.426972173946556 31.182235238357965 21.44217312464514 66-67 21.06284127679926 26.49094471919312 30.748126377095936 21.69808762691168 68-69 21.143919732170204 26.719608799579387 30.28945103935645 21.84702042889396 70-71 21.251749234786473 26.864886856062125 30.025329957722253 21.85803395142915 72-73 21.317098815018937 26.911862640538953 29.76417090933625 22.006867635105863 74-75 21.40273556879118 26.9761832018564 29.57886157679462 22.042219652557804 76-77 21.47472498850631 27.04709225730928 29.360865497422452 22.117317256761957 78-79 21.51734458940597 26.970448381447127 29.232097969405775 22.280109059741136 80-81 21.55046621285559 27.024929770320277 29.14017410576878 22.284429911055355 82-83 21.583053808458033 27.177381304114697 28.98858421696666 22.25098067046061 84-85 21.641227629821802 27.154495873057815 28.869368868837014 22.334907628283368 86-87 21.712669267109845 27.22668110057999 28.718577955925497 22.342071676384666 88-89 21.708571583753006 27.302247876993885 28.59812497548347 22.391055563769644 90-91 21.802055544849733 27.22604435751048 28.46815361832458 22.503746479315208 92-93 21.75864929920009 27.194836701155467 28.49209831712353 22.55441568252091 94-95 21.81381028028025 27.251882664672085 28.37516324097806 22.559143814069607 96-97 21.826435749560666 27.244157378552448 28.369577930178103 22.559828941708787 98-99 21.90395023113416 27.301272757621664 28.273358635258617 22.521418375985558 100-101 22.05848251535001 27.540244747283023 27.80227512465472 22.598997612712246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2354.0 1 1449.5 2 468.5 3 347.5 4 288.0 5 263.0 6 261.5 7 267.5 8 270.0 9 279.0 10 311.5 11 331.5 12 455.0 13 825.5 14 1275.0 15 2583.5 16 67047.0 17 82659.0 18 35128.0 19 35093.5 20 35970.5 21 39414.0 22 45388.0 23 52484.0 24 59537.0 25 69125.0 26 81866.0 27 97135.0 28 113975.0 29 137080.0 30 167917.5 31 199963.0 32 233144.0 33 273221.0 34 309772.5 35 346283.0 36 388301.0 37 420629.0 38 444136.0 39 465184.0 40 482151.5 41 482519.0 42 474615.0 43 475088.5 44 473571.0 45 471702.0 46 462834.5 47 449225.0 48 438325.0 49 423487.0 50 403595.0 51 382173.5 52 367513.0 53 349879.5 54 323467.5 55 292336.0 56 260244.5 57 229324.0 58 200707.0 59 169876.0 60 135366.0 61 106990.5 62 85936.0 63 66812.0 64 51385.0 65 39198.0 66 28989.5 67 21215.5 68 15935.0 69 12135.5 70 9266.5 71 7481.0 72 6375.0 73 5026.0 74 3763.0 75 2776.5 76 1933.5 77 1324.0 78 924.5 79 643.0 80 385.0 81 225.0 82 140.0 83 79.0 84 39.0 85 22.0 86 8.0 87 5.0 88 3.0 89 1.5 90 0.5 91 0.5 92 0.5 93 1.0 94 2.5 95 2.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.046130072934146E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.413839021880244E-5 28-29 0.0 30-31 0.0029810911920221013 32-33 0.006002413034408873 34-35 0.0014241650229093437 36-37 0.001810379266410183 38-39 0.0031701752487360534 40-41 0.0029448836066938975 42-43 0.0023414238512238367 44-45 0.006782887651483485 46-47 0.0014764648683834158 48-49 3.5000665817263535E-4 50-51 0.0026833843793235375 52-53 0.0018667021769207218 54-55 5.55182975032456E-4 56-57 0.0 58-59 6.436904058347317E-5 60-61 4.908139344489829E-4 62-63 0.0 64-65 4.023065036467073E-6 66-67 5.632291051053902E-5 68-69 0.005962182384044203 70-71 0.006098966595284082 72-73 0.0026592459891047354 74-75 0.003427651411069946 76-77 0.03320637881099922 78-79 0.05688211655060795 80-81 0.048747479046871524 82-83 8.287513975122171E-4 84-85 0.0010138123891897024 86-87 0.0015850876243680267 88-89 0.004002949711284738 90-91 0.0 92-93 1.206919510940122E-5 94-95 5.2299845474071954E-5 96-97 0.00610298966032055 98-99 0.0013155422669247328 100-101 4.023065036467073E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2428335E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.06838966473807 #Duplication Level Percentage of deduplicated Percentage of total 1 79.58413114262876 36.66312764608238 2 11.38238272489732 10.487360853675058 3 3.6638987431736783 5.063697449780073 4 1.6054231116150204 2.9583702993062815 5 0.8361519460307085 1.926008683433586 6 0.5330276402851606 1.473343502083957 7 0.3546617038415982 1.1437085500214275 8 0.26092570718909736 0.9616341721867747 9 0.18655567548918245 0.7734887597343674 >10 1.2675857317321313 11.945692964567973 >50 0.1948630849832675 6.320332158429263 >100 0.12812706877138338 10.046429846282901 >500 0.0010279598390277058 0.3101838905805382 >1k 9.725264499400305E-4 0.8231220514705891 >5k 1.7682204927010482E-4 0.613158716754202 >10k+ 8.841102463505241E-5 8.490340455610584 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 925101 7.443482976601451 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 80661 0.6490088978129411 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 14022 0.11282283588268259 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.827678043760488E-5 0.0 0.0 0.0 0.0 2 4.827678043760488E-5 0.0 0.0 0.0 0.0 3 4.827678043760488E-5 0.0 8.046130072934146E-6 0.0 0.0 4 4.827678043760488E-5 0.0 8.046130072934146E-6 8.046130072934146E-6 8.046130072934146E-6 5 4.827678043760488E-5 0.0 8.046130072934146E-6 8.046130072934146E-6 8.046130072934146E-6 6 4.827678043760488E-5 0.0 8.046130072934146E-6 8.046130072934146E-6 8.046130072934146E-6 7 4.827678043760488E-5 0.0 8.046130072934146E-6 8.046130072934146E-6 8.046130072934146E-6 8 4.827678043760488E-5 0.0 8.046130072934146E-6 8.046130072934146E-6 8.046130072934146E-6 9 6.436904058347317E-5 0.0 8.046130072934146E-6 2.413839021880244E-5 8.046130072934146E-6 10-11 7.643823569287439E-5 0.0 8.046130072934146E-6 3.218452029173658E-5 8.046130072934146E-6 12-13 8.448436576580852E-5 0.0 8.046130072934146E-6 4.023065036467073E-5 8.046130072934146E-6 14-15 1.0057662591167682E-4 0.0 8.046130072934146E-6 4.023065036467073E-5 1.609226014586829E-5 16-17 1.1666888605754513E-4 0.0 8.046130072934146E-6 4.023065036467073E-5 2.0115325182335364E-5 18-19 1.5689953642221584E-4 0.0 8.046130072934146E-6 4.023065036467073E-5 2.413839021880244E-5 20-21 1.8103792664101828E-4 0.0 1.206919510940122E-5 4.827678043760488E-5 3.218452029173658E-5 22-23 2.0115325182335365E-4 4.023065036467073E-6 1.609226014586829E-5 4.827678043760488E-5 4.42537154011378E-5 24-25 2.2126857700568902E-4 8.046130072934146E-6 1.609226014586829E-5 4.827678043760488E-5 4.827678043760488E-5 26-27 2.413839021880244E-4 1.206919510940122E-5 1.609226014586829E-5 4.827678043760488E-5 5.229984547407195E-5 28-29 2.655222924068268E-4 1.609226014586829E-5 1.609226014586829E-5 5.632291051053902E-5 6.03459755470061E-5 30-31 2.856376175891622E-4 2.0115325182335364E-5 1.609226014586829E-5 5.632291051053902E-5 8.046130072934145E-5 32-33 3.0575294277149754E-4 2.413839021880244E-5 1.609226014586829E-5 5.632291051053902E-5 8.85074308022756E-5 34-35 3.137990728444317E-4 2.413839021880244E-5 1.609226014586829E-5 5.632291051053902E-5 8.85074308022756E-5 36-37 3.540297232091024E-4 2.413839021880244E-5 1.609226014586829E-5 5.632291051053902E-5 8.85074308022756E-5 38-39 3.821911784643719E-4 2.413839021880244E-5 1.609226014586829E-5 6.03459755470061E-5 8.85074308022756E-5 40-41 4.3046795890197683E-4 2.413839021880244E-5 1.609226014586829E-5 6.436904058347317E-5 8.85074308022756E-5 42-43 4.7874473933958166E-4 2.413839021880244E-5 1.609226014586829E-5 6.436904058347317E-5 8.85074308022756E-5 44-45 5.189753897042525E-4 2.413839021880244E-5 1.609226014586829E-5 6.436904058347317E-5 8.85074308022756E-5 46-47 5.712752351783244E-4 2.413839021880244E-5 2.413839021880244E-5 6.436904058347317E-5 8.85074308022756E-5 48-49 6.195520156159292E-4 2.413839021880244E-5 2.413839021880244E-5 8.046130072934145E-5 9.253049583874269E-5 50-51 6.839210561994024E-4 2.413839021880244E-5 2.413839021880244E-5 9.253049583874269E-5 9.655356087520976E-5 52-53 9.212818933509598E-4 2.413839021880244E-5 2.413839021880244E-5 1.0057662591167682E-4 1.0057662591167682E-4 54-55 0.0014845109984563498 2.413839021880244E-5 2.413839021880244E-5 1.045996909481439E-4 1.045996909481439E-4 56-57 0.002248893355385094 2.413839021880244E-5 2.816145525526951E-5 1.0862275598461096E-4 1.1264582102107804E-4 58-59 0.0032345442893195265 2.413839021880244E-5 3.218452029173658E-5 1.1264582102107804E-4 1.1264582102107804E-4 60-61 0.004296633458946834 2.413839021880244E-5 3.218452029173658E-5 1.1264582102107804E-4 1.1264582102107804E-4 62-63 0.005652406376236238 2.413839021880244E-5 3.218452029173658E-5 1.206919510940122E-4 1.1264582102107804E-4 64-65 0.0076599158294333065 2.816145525526951E-5 3.218452029173658E-5 1.2471501613047926E-4 1.206919510940122E-4 66-67 0.009900763054745467 3.218452029173658E-5 3.218452029173658E-5 1.2873808116694633E-4 1.206919510940122E-4 68-69 0.012230117710859901 3.218452029173658E-5 3.218452029173658E-5 1.2873808116694633E-4 1.206919510940122E-4 70-71 0.015488800390398232 3.218452029173658E-5 3.218452029173658E-5 1.3678421123988047E-4 1.206919510940122E-4 72-73 0.019938310320730814 4.023065036467073E-5 3.218452029173658E-5 1.4080727627634757E-4 1.206919510940122E-4 74-75 0.02567520106273286 4.827678043760488E-5 3.218452029173658E-5 1.4483034131281463E-4 1.206919510940122E-4 76-77 0.03240981193377874 4.827678043760488E-5 3.218452029173658E-5 1.4483034131281463E-4 1.206919510940122E-4 78-79 0.04068525671379151 4.827678043760488E-5 3.218452029173658E-5 1.4483034131281463E-4 1.206919510940122E-4 80-81 0.05083544980079793 5.632291051053902E-5 3.218452029173658E-5 1.488534063492817E-4 1.206919510940122E-4 82-83 0.06386213438887832 5.632291051053902E-5 3.218452029173658E-5 1.5689953642221584E-4 1.206919510940122E-4 84-85 0.07984979484379848 6.436904058347317E-5 4.023065036467073E-5 1.6494566649514998E-4 1.206919510940122E-4 86-87 0.10063697188722384 6.436904058347317E-5 4.827678043760488E-5 1.7299179656808414E-4 1.2471501613047926E-4 88-89 0.12611101969813332 6.436904058347317E-5 5.632291051053902E-5 1.8908405671395244E-4 1.2873808116694633E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 87955 0.0 94.2575 1 TATCTCG 115635 0.0 92.5468 2 ATATCTC 116125 0.0 92.23399 1 TCGACGG 90845 0.0 91.24848 2 CGACGGC 91460 0.0 90.53624 3 ATCTCGA 118235 0.0 90.44742 3 GACGGCG 92250 0.0 89.76091 4 ACGGCGC 92320 0.0 89.63113 5 CTCGAGG 116795 0.0 89.61478 5 TCTCGAG 116815 0.0 89.58317 4 TCGAGGG 116885 0.0 89.57015 6 CGGCGCG 93005 0.0 89.06288 6 AGGGCGC 117920 0.0 88.84843 9 CGAGGGC 117975 0.0 88.65 7 GAGGGCG 119520 0.0 87.71465 8 CTCGACG 3040 0.0 75.14553 5 TCTCGAC 4400 0.0 51.91873 4 GGGCGCG 118445 0.0 44.297478 10-11 GGATCCT 122225 0.0 42.45928 18-19 GATCCTT 120675 0.0 42.406425 20-21 >>END_MODULE