FastQCFastQC Report
Tue 31 May 2016
SRR1051288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12428335
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA608820.4898644911003767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG74450.087.827131
TCGACGG76250.085.844962
CGACGGC83350.078.758493
ACGGCGC89900.072.861155
GACGGCG90000.072.780494
CGGCGCG100300.065.25896
CGCGCCG140100.049.0932669
GGCGCGC146250.047.158747
GCGCGCC147850.046.6484038
TATCTCG20450.029.2673022
GCCGGAT139900.025.85494812-13
CGCCGGA136850.025.70228410-11
ATCTCGA45250.025.6239571
TCGAGGG46700.024.7162514
CCGGATC144100.024.50803212-13
GCGCCGG150700.024.4590710-11
GGGCGCG49550.023.1986758
CTCGAGG50100.022.9441853
CGGATCC162950.022.40170114-15
TCCATAT154800.021.54059218-19
Produced by FastQC (version 0.11.5)