##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051288_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12428335 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.247213162503265 33.0 31.0 34.0 31.0 34.0 2 32.517542293476964 34.0 31.0 34.0 31.0 34.0 3 32.64994844442156 34.0 31.0 34.0 31.0 34.0 4 36.17071273022493 37.0 35.0 37.0 35.0 37.0 5 36.07668911402855 37.0 35.0 37.0 35.0 37.0 6 36.08993996380046 37.0 35.0 37.0 35.0 37.0 7 36.058367110316865 37.0 35.0 37.0 35.0 37.0 8 36.06939698680475 37.0 35.0 37.0 35.0 37.0 9 37.85385041520043 39.0 38.0 39.0 35.0 39.0 10-11 37.8217274880344 39.0 38.0 39.0 35.0 39.0 12-13 37.801181493739904 39.0 38.0 39.0 35.0 39.0 14-15 39.17977476468087 41.0 39.0 41.0 36.0 41.0 16-17 39.13809693736128 40.0 38.5 41.0 36.0 41.0 18-19 39.10338923113997 40.0 38.5 41.0 36.0 41.0 20-21 39.07812442294161 40.0 39.0 41.0 35.5 41.0 22-23 38.98194343811943 40.0 38.0 41.0 35.0 41.0 24-25 38.89862020938445 40.0 38.0 41.0 35.0 41.0 26-27 38.73917310725854 40.0 38.0 41.0 35.0 41.0 28-29 38.5627283542003 40.0 38.0 41.0 34.0 41.0 30-31 38.40946341565463 40.0 38.0 41.0 34.0 41.0 32-33 38.583678505608354 40.0 38.0 41.0 34.0 41.0 34-35 38.556989974924235 40.0 38.0 41.0 34.0 41.0 36-37 38.497420410698616 40.0 38.0 41.0 34.0 41.0 38-39 38.41272704670416 40.0 38.0 41.0 34.0 41.0 40-41 38.24450978349071 40.0 38.0 41.0 33.0 41.0 42-43 38.16000485986257 40.0 38.0 41.0 33.0 41.0 44-45 38.1086049338065 40.0 38.0 41.0 33.0 41.0 46-47 38.017903444025286 40.0 37.0 41.0 33.0 41.0 48-49 37.94631006486388 40.0 37.0 41.0 33.0 41.0 50-51 37.98518751707289 40.0 37.0 41.0 33.0 41.0 52-53 37.886755184825645 40.0 37.0 41.0 33.0 41.0 54-55 37.64673051539084 40.0 37.0 41.0 32.5 41.0 56-57 37.48995404452809 39.5 36.0 41.0 32.0 41.0 58-59 37.29344847881876 39.0 36.0 41.0 32.0 41.0 60-61 37.075851391196004 39.0 35.5 41.0 31.5 41.0 62-63 36.77233985887892 39.0 35.0 40.5 31.0 41.0 64-65 36.43749343737515 38.0 35.0 40.0 31.0 41.0 66-67 36.066926382335204 37.5 35.0 40.0 31.0 41.0 68-69 35.69940072423216 37.0 35.0 39.5 30.0 41.0 70-71 35.27618096068379 36.5 34.0 39.0 30.0 41.0 72-73 34.86646722992259 36.0 34.0 39.0 29.0 40.0 74-75 34.47533297903541 35.5 34.0 38.0 29.0 40.0 76-77 33.84308115286561 35.0 33.0 37.0 29.0 39.0 78-79 33.69252651300435 35.0 33.0 37.0 29.0 39.0 80-81 33.38905818840577 35.0 33.0 36.0 29.0 38.5 82-83 33.034497259689246 35.0 33.0 36.0 28.0 37.0 84-85 32.74543295622463 35.0 33.0 35.5 27.5 37.0 86-87 32.40382585438838 35.0 33.0 35.0 26.5 36.5 88-89 32.18857871951472 35.0 33.0 35.0 26.0 36.0 90-91 31.92490277257573 35.0 32.0 35.0 26.0 36.0 92-93 31.63549333036163 34.5 32.0 35.0 25.5 35.5 94-95 31.345942236027593 34.0 32.0 35.0 25.0 35.0 96-97 31.096727477976735 34.0 32.0 35.0 24.5 35.0 98-99 30.724881289408437 34.0 32.0 35.0 23.0 35.0 100-101 29.061080748145265 33.0 29.5 34.5 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41276786180961267 1101 2 0.3878950866261519 1101 3 0.3528901650890859 1101 4 0.24429438712805762 1101 5 0.26084213183433747 1101 6 0.23288338877279813 1101 7 0.27341827158640086 1101 8 0.2379281834166349 1101 9 0.30998454535179576 1101 10-11 0.31114283249218744 1101 12-13 0.3334312042276082 1101 14-15 0.42686351280173795 1101 16-17 0.36040453350251767 1101 18-19 0.36134063082170087 1101 20-21 0.3902699187597065 1101 22-23 0.40035900526343937 1101 24-25 0.4053508415846778 1101 26-27 0.41418850931438556 1101 28-29 0.43656569551214375 1101 30-31 0.4046917581254661 1101 32-33 0.3795713035510673 1101 34-35 0.40005120080346757 1101 36-37 0.4010861989622754 1101 38-39 0.3790680460359823 1101 40-41 0.3631668189587387 1101 42-43 0.39143374959245136 1101 44-45 0.34651252117750175 1101 46-47 0.3442139689029702 1101 48-49 0.4013948481681169 1101 50-51 0.4705875298791824 1101 52-53 0.44522430315352324 1101 54-55 0.5458440792360406 1101 56-57 0.43677744858772627 1101 58-59 0.38803399335443345 1101 60-61 0.30595804693177797 1101 62-63 0.41997948566707777 1101 64-65 0.41284542745171393 1101 66-67 0.27657278676466035 1101 68-69 0.22377047324280852 1101 70-71 0.2936633084643603 1101 72-73 0.2474632983687144 1101 74-75 0.22577129499052973 1101 76-77 0.2330448048422511 1101 78-79 0.2366092155978592 1101 80-81 0.1839088718410622 1101 82-83 0.03851206612331026 1101 84-85 0.06723842352388942 1101 86-87 0.27364090394746654 1101 88-89 0.254560309135357 1101 90-91 0.18829422045343946 1101 92-93 0.05703558207633819 1101 94-95 0.12344759728107846 1101 96-97 0.21966892628260126 1101 98-99 0.3436690560859397 1101 100-101 0.4274248945745889 1102 1 0.3598274904638359 1102 2 0.33639528485343106 1102 3 0.27254003435347585 1102 4 0.17719451125608998 1102 5 0.20435077831957926 1102 6 0.21862895933698923 1102 7 0.22033583735537832 1102 8 0.21060137270480794 1102 9 0.2694193970117169 1102 10-11 0.25594234565541996 1102 12-13 0.2169546528631443 1102 14-15 0.3149450679891501 1102 16-17 0.3059489623719571 1102 18-19 0.31322122366285754 1102 20-21 0.28049318403500223 1102 22-23 0.28099302578468155 1102 24-25 0.30919058072701233 1102 26-27 0.313496808193932 1102 28-29 0.3718221874556207 1102 30-31 0.3324744325890734 1102 32-33 0.3108876152965081 1102 34-35 0.382504605593013 1102 36-37 0.35225461899644017 1102 38-39 0.33292038087018483 1102 40-41 0.3609625138869532 1102 42-43 0.3202400968772139 1102 44-45 0.32822145273244985 1102 46-47 0.3021952128263621 1102 48-49 0.3460564576792464 1102 50-51 0.44200654813497664 1102 52-53 0.4562278936631543 1102 54-55 0.5103226057937817 1102 56-57 0.3721363284619983 1102 58-59 0.3541472755262447 1102 60-61 0.34377951730623835 1102 62-63 0.3717892830504752 1102 64-65 0.3846926402345403 1102 66-67 0.36019694193885954 1102 68-69 0.35909764265161925 1102 70-71 0.39710883489938453 1102 72-73 0.3741564307373437 1102 74-75 0.34768986369516597 1102 76-77 0.3544665369239226 1102 78-79 0.36178163203562264 1102 80-81 0.4265449348112682 1102 82-83 0.30233284388221193 1102 84-85 0.3141390573792222 1102 86-87 0.5153490154550298 1102 88-89 0.46790563639915916 1102 90-91 0.3812874465080647 1102 92-93 0.33004962199553844 1102 94-95 0.4255591308050839 1102 96-97 0.4236985152441761 1102 98-99 0.5308273795079081 1102 100-101 0.670243993301213 1103 1 0.2740788478744989 1103 2 0.26736206004643037 1103 3 0.21528402747082254 1103 4 0.1525422659934108 1103 5 0.18374839211109872 1103 6 0.15495461944146882 1103 7 0.16073419801266198 1103 8 0.14939518550376363 1103 9 0.14816391956911446 1103 10-11 0.12993140540009307 1103 12-13 0.2018426149079673 1103 14-15 0.2508823695249802 1103 16-17 0.24185315030814536 1103 18-19 0.2216845938679981 1103 20-21 0.24750911955347732 1103 22-23 0.1860855861036299 1103 24-25 0.16288475069068653 1103 26-27 0.19194581127847954 1103 28-29 0.23484668401901132 1103 30-31 0.2160134054241425 1103 32-33 0.16306431555887713 1103 34-35 0.2309826129311503 1103 36-37 0.24999845553356437 1103 38-39 0.21632401209539864 1103 40-41 0.21449958197161578 1103 42-43 0.3048537494121746 1103 44-45 0.2123167977523437 1103 46-47 0.2396971612304668 1103 48-49 0.30564320757401475 1103 50-51 0.3117332311987937 1103 52-53 0.33205367822279186 1103 54-55 0.4047779432479288 1103 56-57 0.36507445072091826 1103 58-59 0.2759150939177246 1103 60-61 0.2567702522065147 1103 62-63 0.2580425783141962 1103 64-65 0.25793991169348374 1103 66-67 0.2822052011496865 1103 68-69 0.2182469185194762 1103 70-71 0.3038204423381572 1103 72-73 0.3484339773284688 1103 74-75 0.3451530356552439 1103 76-77 0.34155742852577475 1103 78-79 0.36980339375428883 1103 80-81 0.392248540949943 1103 82-83 0.3024077782230208 1103 84-85 0.313346317314938 1103 86-87 0.5285332246915218 1103 88-89 0.48362732089631066 1103 90-91 0.3453146851974047 1103 92-93 0.28932657816628904 1103 94-95 0.38580417245373866 1103 96-97 0.4845831078982741 1103 98-99 0.49821631221529117 1103 100-101 0.6346266160108982 1104 1 0.40012563279294255 1104 2 0.3903462979220649 1104 3 0.28816721964831515 1104 4 0.19769859350036967 1104 5 0.21626790058710554 1104 6 0.18382425090640453 1104 7 0.21890603016082366 1104 8 0.20897511601837948 1104 9 0.2668518742392436 1104 10-11 0.25556366527835195 1104 12-13 0.2572023631282434 1104 14-15 0.36986354230143803 1104 16-17 0.2812034782695534 1104 18-19 0.3484360863922902 1104 20-21 0.3594077566026854 1104 22-23 0.35794877952407944 1104 24-25 0.3704804666148931 1104 26-27 0.3709331111064458 1104 28-29 0.38129229035179435 1104 30-31 0.3820303352690928 1104 32-33 0.3360024709875873 1104 34-35 0.37413966589580383 1104 36-37 0.3650552247488221 1104 38-39 0.4009546258593275 1104 40-41 0.4054991916484951 1104 42-43 0.4103581368550522 1104 44-45 0.32923503221133643 1104 46-47 0.35473549036448304 1104 48-49 0.37297622500495464 1104 50-51 0.3969320324190093 1104 52-53 0.42148658716335063 1104 54-55 0.3974640471574915 1104 56-57 0.2979841660985656 1104 58-59 0.38189112604980124 1104 60-61 0.26710833436357717 1104 62-63 0.25466028935320395 1104 64-65 0.31303404118449407 1104 66-67 0.21743060842086237 1104 68-69 0.23771717203560172 1104 70-71 0.3359376928245652 1104 72-73 0.32789566886977184 1104 74-75 0.3408302515764845 1104 76-77 0.24046793551262624 1104 78-79 0.2256769285999951 1104 80-81 0.29331798087387284 1104 82-83 0.15228570395068886 1104 84-85 0.13529679820438645 1104 86-87 0.36262616646627777 1104 88-89 0.4033992519325764 1104 90-91 0.32890658197136347 1104 92-93 0.23942550178956523 1104 94-95 0.37271191867369424 1104 96-97 0.38874532028635755 1104 98-99 0.5007423119397281 1104 100-101 0.6540783173015434 1105 1 -0.09525113765690207 1105 2 0.06375533688076729 1105 3 0.04373507754846884 1105 4 0.042384134448489874 1105 5 0.008339888757561198 1105 6 0.04100787347137924 1105 7 0.06491670749502987 1105 8 -0.027996146085278895 1105 9 -0.01346257866003242 1105 10-11 -0.03862634278304 1105 12-13 0.017734062028665676 1105 14-15 -9.169930423880146E-4 1105 16-17 0.009317550622647275 1105 18-19 -0.02917979330168663 1105 20-21 -0.007839834380611421 1105 22-23 0.014539191014442565 1105 24-25 -0.019751612462563628 1105 26-27 -0.020062060548362126 1105 28-29 -0.048952818964792755 1105 30-31 -0.006722623027840768 1105 32-33 0.0838163263455769 1105 34-35 0.05757505622807457 1105 36-37 0.09343230570847538 1105 38-39 0.09813404705445805 1105 40-41 0.08237377007179703 1105 42-43 0.10320596211488464 1105 44-45 0.08128309079774709 1105 46-47 -0.11195812964690788 1105 48-49 0.12371103712005294 1105 50-51 0.1126417335263028 1105 52-53 0.10969411861498912 1105 54-55 0.11034541901974393 1105 56-57 -0.003361955602578348 1105 58-59 0.11677174736771434 1105 60-61 0.10463432851672394 1105 62-63 0.1762313043779713 1105 64-65 0.19778741057407956 1105 66-67 0.22143780810419855 1105 68-69 0.19082300973827415 1105 70-71 0.10920964921803034 1105 72-73 0.1544165332491616 1105 74-75 0.20911114537125286 1105 76-77 0.17983387075233281 1105 78-79 0.2719232766580504 1105 80-81 0.3035838378918001 1105 82-83 0.23338466542492853 1105 84-85 0.17281080215524014 1105 86-87 0.3822149133562647 1105 88-89 0.3412211256152986 1105 90-91 0.2966067901706957 1105 92-93 0.17574890473990123 1105 94-95 0.31171221365432444 1105 96-97 0.3809272014171867 1105 98-99 0.4912119776631272 1105 100-101 0.6623439922514862 1106 1 -0.175925582085938 1106 2 -0.061509333264091026 1106 3 -0.04677156412338945 1106 4 -0.011813792043689375 1106 5 -0.3740546157581619 1106 6 -0.25365580719952874 1106 7 -0.16763422727667887 1106 8 -0.33801653930036935 1106 9 -0.5203822965334126 1106 10-11 -0.1262307092957613 1106 12-13 -0.11068766791147766 1106 14-15 -0.2753818473921612 1106 16-17 -0.13346239980397456 1106 18-19 -0.22552082190359357 1106 20-21 -0.18882728369725754 1106 22-23 -0.1562902789261571 1106 24-25 -0.116016673358601 1106 26-27 -0.12180462826234617 1106 28-29 -0.16830546340231223 1106 30-31 -0.21660399362097138 1106 32-33 -0.17987163975452347 1106 34-35 -0.12055540043258617 1106 36-37 -0.0978166088438499 1106 38-39 -0.12248563545763602 1106 40-41 -0.10654320884179214 1106 42-43 -0.21959426856055586 1106 44-45 -0.15425041081309132 1106 46-47 -0.22266482444901214 1106 48-49 -0.06889870247095331 1106 50-51 -0.11802233620627334 1106 52-53 -0.16740266171724727 1106 54-55 -0.12045389345724544 1106 56-57 -0.22317989436700003 1106 58-59 -0.14984303775315055 1106 60-61 -0.24839883853424993 1106 62-63 -0.17841738211303948 1106 64-65 -0.23136995250224146 1106 66-67 -0.22124357214674006 1106 68-69 -0.23921023360141902 1106 70-71 -0.16415351135800194 1106 72-73 -0.12419337116048723 1106 74-75 -0.1508876805781938 1106 76-77 -0.23478869964151272 1106 78-79 -0.18130137014342296 1106 80-81 -0.1758166088648423 1106 82-83 -0.28240070266748063 1106 84-85 -0.3028790961519192 1106 86-87 -0.08580033982434543 1106 88-89 -0.015079316301687129 1106 90-91 -0.12415068969017184 1106 92-93 -0.320019318052136 1106 94-95 -0.20190068094412084 1106 96-97 -0.16815437626592455 1106 98-99 -0.02950349272144237 1106 100-101 -0.03790988926883898 1107 1 -0.186769349404031 1107 2 -0.15011998745350041 1107 3 -0.1939394614206904 1107 4 -0.14183752096054292 1107 5 -0.1890778588089148 1107 6 -0.1853832161357971 1107 7 -0.1384271406216726 1107 8 -0.14063545095628172 1107 9 -0.23915924196148808 1107 10-11 -0.27819121060255725 1107 12-13 -0.29662894460076217 1107 14-15 -0.2998109501261226 1107 16-17 -0.29318910397891784 1107 18-19 -0.311450574982409 1107 20-21 -0.3016176577904801 1107 22-23 -0.33967678357457487 1107 24-25 -0.4043863817551525 1107 26-27 -0.4217374306232884 1107 28-29 -0.39088184604653264 1107 30-31 -0.4928604690345608 1107 32-33 -0.5147856363632286 1107 34-35 -0.43131042306221445 1107 36-37 -0.4108068957646793 1107 38-39 -0.2901493964678039 1107 40-41 -0.33219740821740373 1107 42-43 -0.37135596218394085 1107 44-45 -0.43476958383991615 1107 46-47 -0.4299662973952181 1107 48-49 -0.35062934497948817 1107 50-51 -0.2353832635224009 1107 52-53 -0.2758746539142436 1107 54-55 -0.37061237252421364 1107 56-57 -0.4708622718367579 1107 58-59 -0.37741440539765847 1107 60-61 -0.45717128884299996 1107 62-63 -0.35431942983002074 1107 64-65 -0.3145317114389172 1107 66-67 -0.3890952257350264 1107 68-69 -0.39745982933380475 1107 70-71 -0.36965593036241984 1107 72-73 -0.38690067013916973 1107 74-75 -0.3459645496449326 1107 76-77 -0.425602838467384 1107 78-79 -0.40640626075696673 1107 80-81 -0.37596023787689603 1107 82-83 -0.3850867014946431 1107 84-85 -0.47510499129289485 1107 86-87 -0.2749330449601004 1107 88-89 -0.2788230585376468 1107 90-91 -0.432787374360629 1107 92-93 -0.5446100019826225 1107 94-95 -0.4768327568452051 1107 96-97 -0.6034712397739384 1107 98-99 -0.5449031331624674 1107 100-101 -0.3029301657112029 1108 1 -0.3666412542585107 1108 2 -0.2793916151919902 1108 3 -0.30056699156705236 1108 4 -0.18097213320786665 1108 5 -0.39386344166568676 1108 6 -0.5473980333626685 1108 7 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-0.0022985626447322716 2305 10-11 0.04920151289098129 2305 12-13 0.04226293492695987 2305 14-15 0.1480403193241031 2305 16-17 0.07263431349147709 2305 18-19 0.11660319654811957 2305 20-21 0.10969552684830575 2305 22-23 0.15585245398762027 2305 24-25 0.06751113416404309 2305 26-27 0.14922820364191836 2305 28-29 0.12677773525504676 2305 30-31 0.17031383458314764 2305 32-33 0.148283567655362 2305 34-35 0.15163045840458977 2305 36-37 0.1875793415751872 2305 38-39 0.15553866398485638 2305 40-41 0.21927533419651724 2305 42-43 0.2754563920452 2305 44-45 0.23341404166814783 2305 46-47 0.29025259774959977 2305 48-49 0.270911355198443 2305 50-51 0.215018128823381 2305 52-53 0.29504142362772967 2305 54-55 0.25663616118233534 2305 56-57 0.2742696318689113 2305 58-59 0.3221300645343277 2305 60-61 0.4106434067007072 2305 62-63 0.3791226982837941 2305 64-65 0.3047289355746301 2305 66-67 0.45394135844155414 2305 68-69 0.4354749411750447 2305 70-71 0.4583583873721295 2305 72-73 0.46856239877561734 2305 74-75 0.421216477192246 2305 76-77 0.4124572109514588 2305 78-79 0.4387508432038132 2305 80-81 0.5099933725011354 2305 82-83 0.5019372760773706 2305 84-85 0.512178918986713 2305 86-87 0.6574724266518004 2305 88-89 0.5635963401140884 2305 90-91 0.6148984770736767 2305 92-93 0.7756363823728272 2305 94-95 0.8326137525327582 2305 96-97 0.834595067152641 2305 98-99 0.9393693627791926 2305 100-101 1.0740443585329942 2306 1 -0.5366427716242299 2306 2 -0.9332309594971626 2306 3 -0.739728975547024 2306 4 -0.3442000345821654 2306 5 -0.23100113562924207 2306 6 -0.20106906735067298 2306 7 -0.08537427554637134 2306 8 -0.05782234394486352 2306 9 -0.08831988978687377 2306 10-11 -0.36240877102041935 2306 12-13 -0.2674469357588052 2306 14-15 -0.14650531009842638 2306 16-17 -0.197103429940654 2306 18-19 -0.15243702947007165 2306 20-21 -0.13951562175868304 2306 22-23 -0.06637203946581849 2306 24-25 -0.10722951703149164 2306 26-27 -0.14150462189905255 2306 28-29 -0.09473972400017772 2306 30-31 -0.05255151426515425 2306 32-33 -0.091022546169782 2306 34-35 -0.1936695747955497 2306 36-37 -0.2272951979146285 2306 38-39 -0.133259609367542 2306 40-41 -0.18801923522629238 2306 42-43 -0.1673743028478185 2306 44-45 -0.1959113046349188 2306 46-47 -0.13950034367264408 2306 48-49 -0.07331139802668218 2306 50-51 -0.07230263519091551 2306 52-53 -0.037415967828565044 2306 54-55 -0.13016729130123394 2306 56-57 -0.12884679489810225 2306 58-59 -0.21267168241575263 2306 60-61 -0.23319703472260755 2306 62-63 -0.04842886089556231 2306 64-65 -0.1076257780671952 2306 66-67 -0.06613068418700863 2306 68-69 -0.0159992858727378 2306 70-71 -0.05802367933674901 2306 72-73 -0.07807543994152866 2306 74-75 -0.07629644412872238 2306 76-77 -0.040879790868814325 2306 78-79 0.011927881026686293 2306 80-81 0.14037103365337344 2306 82-83 0.14663288201802516 2306 84-85 0.035636093214542086 2306 86-87 0.18010269235212917 2306 88-89 0.0620700393947331 2306 90-91 0.11900447507413148 2306 92-93 0.16022564067838374 2306 94-95 0.2905538099952736 2306 96-97 0.15892019354082265 2306 98-99 0.2658632614201828 2306 100-101 0.5781439920053373 2307 1 -1.6812630757735434 2307 2 -2.179242610802291 2307 3 -1.7719295715779566 2307 4 -0.9237809766059328 2307 5 -0.5205853102438169 2307 6 -0.43508239148530947 2307 7 -0.3689030449833339 2307 8 -0.4756759963168946 2307 9 -0.4249751968270914 2307 10-11 -0.35926229375748164 2307 12-13 -0.35689888555362614 2307 14-15 -0.6430500023748422 2307 16-17 -0.5407283042402895 2307 18-19 -0.39186179034947344 2307 20-21 -0.4193212932988786 2307 22-23 -0.5082542410921036 2307 24-25 -0.45401383856223987 2307 26-27 -0.4186099735038695 2307 28-29 -0.37571268749903197 2307 30-31 -0.7658997936715863 2307 32-33 -0.4859023584020221 2307 34-35 -0.49666607620455494 2307 36-37 -0.4150557440091802 2307 38-39 -0.410862212545382 2307 40-41 -0.3494505155434737 2307 42-43 -0.3673650759340177 2307 44-45 -0.3359617846156411 2307 46-47 -0.4506175516999136 2307 48-49 -0.4402118891071325 2307 50-51 -0.3617139666331539 2307 52-53 -0.3851383231199037 2307 54-55 -0.32761655505330367 2307 56-57 -0.24459545418054773 2307 58-59 -0.4400642047249832 2307 60-61 -0.44866910791294146 2307 62-63 -0.3119194177854254 2307 64-65 -0.2809634179839122 2307 66-67 -0.3296270394460521 2307 68-69 -0.21242915102545368 2307 70-71 -0.29841042072369106 2307 72-73 -0.3425797213123616 2307 74-75 -0.3300802695882936 2307 76-77 -0.2818066516379574 2307 78-79 -0.2967066853505571 2307 80-81 -0.2566599851884277 2307 82-83 -0.2287557950923329 2307 84-85 -0.2722123553800202 2307 86-87 -0.24071059648493076 2307 88-89 -0.2584227342323757 2307 90-91 -0.23783567724488464 2307 92-93 -0.22774455011281347 2307 94-95 -0.1689239376240792 2307 96-97 -0.18390541051464027 2307 98-99 -0.19976987829939574 2307 100-101 -0.020218630081394906 2308 1 -1.2625814440243808 2308 2 -1.2425995489325992 2308 3 -0.7702551379654388 2308 4 -0.5141261577353191 2308 5 -0.6479063460504051 2308 6 -0.5714963842734733 2308 7 -1.4603878837761854 2308 8 -0.5917109147863826 2308 9 -0.5650070534634679 2308 10-11 -0.8210828157309962 2308 12-13 -0.8988310351671132 2308 14-15 -0.6607159481581419 2308 16-17 -0.8386284725965254 2308 18-19 -0.5891341314109582 2308 20-21 -0.6829040246832179 2308 22-23 -0.5264869065229618 2308 24-25 -0.5356318274091052 2308 26-27 -0.522631741252674 2308 28-29 -1.3171321403476384 2308 30-31 -0.6668605401543459 2308 32-33 -0.4960773686377209 2308 34-35 -1.1101218431461959 2308 36-37 -0.6579908909607042 2308 38-39 -0.4888637026274836 2308 40-41 -0.9937673660451978 2308 42-43 -0.4785844192538491 2308 44-45 -0.5600679396490662 2308 46-47 -0.5286015895907781 2308 48-49 -0.3805569665970481 2308 50-51 -0.49432607834649644 2308 52-53 -0.575346155976483 2308 54-55 -1.0440436999639005 2308 56-57 -1.7509404646105011 2308 58-59 -1.1656035762271841 2308 60-61 -0.7629708863523703 2308 62-63 -0.5202889708775089 2308 64-65 -0.40379590342706706 2308 66-67 -0.40244065629184433 2308 68-69 -0.30985627515347147 2308 70-71 -0.2048262476074214 2308 72-73 -0.31020691438324377 2308 74-75 -0.17220035559105895 2308 76-77 -1.2215080093289643 2308 78-79 -0.6867481690252077 2308 80-81 -0.21297703309227956 2308 82-83 -0.07213116201085512 2308 84-85 0.002994587916852254 2308 86-87 -0.3773299998490387 2308 88-89 -0.3307584997927506 2308 90-91 -0.9284253302199694 2308 92-93 -0.12384383426180534 2308 94-95 0.03712275230117257 2308 96-97 -0.09755559006990211 2308 98-99 -0.518285597295673 2308 100-101 0.18996400113502432 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 25.0 12 149.0 13 939.0 14 3263.0 15 7222.0 16 12246.0 17 18144.0 18 25904.0 19 33949.0 20 37667.0 21 39783.0 22 43133.0 23 49315.0 24 58184.0 25 71605.0 26 89683.0 27 112932.0 28 142275.0 29 178130.0 30 222650.0 31 277620.0 32 347140.0 33 450920.0 34 620173.0 35 923608.0 36 1509110.0 37 2896413.0 38 3550009.0 39 705748.0 40 394.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.974994435650153 32.59098265412042 24.30306769751027 19.130955212719158 2 23.177542826571845 30.810043637026645 27.318067682651247 18.694345853750264 3 23.140974713062448 28.502739792380527 28.07449988791722 20.281785606639808 4 24.161039534926946 28.14537306590018 27.371753253676058 20.321834145496815 5 23.68205395171598 29.43125527272961 26.005760224519214 20.880930551035195 6 23.21969113320489 28.33607237011233 25.123823907224903 23.32041258945788 7 23.603226015391442 27.990249699577618 26.79504535402369 21.611478931007248 8 22.791363444902313 28.084767589544374 26.501200683760135 22.622668281793175 9 22.409799864583633 27.467838612332223 26.9896410098376 23.132720513246547 10-11 22.724013313126818 28.1545919063173 26.902247968050425 22.219146812505457 12-13 22.579794477699547 27.971759692670016 27.3081913224901 22.140254507140337 14-15 22.54097994622771 27.85168729359162 27.302784323081084 22.30454843709958 16-17 22.73277957184128 28.161101225546304 27.090201543489133 22.015917659123286 18-19 22.415114333496806 27.86437604071664 27.399720075134766 22.320789550651796 20-21 22.407277402805768 28.03404076249956 27.123106192422398 22.435575642272276 22-23 22.430824402464207 28.066708050595672 27.434789133057645 22.067678413882472 24-25 22.451050156843227 27.70970348881837 27.789400413603303 22.049845940735096 26-27 22.456668572258472 27.737404085100696 27.794748854130503 22.01117848851033 28-29 22.463942273844406 27.8131060998919 27.761558567579648 21.96139305868405 30-31 22.522920407279013 27.75056353083498 27.728613687996017 21.997902373889985 32-33 22.525821037170303 27.806198497224287 27.696046976525817 21.97193348907959 34-35 22.538606337856198 27.844260715534304 27.694208435804153 21.92292451080535 36-37 22.55771187371438 27.82263674096329 27.648256986957627 21.971394398364705 38-39 22.55706416024351 27.849695876398567 27.564428380792762 22.028811582565165 40-41 22.624398199758858 27.962023875281766 27.355056007099904 22.058521917859473 42-43 22.631627647629387 27.993745743094305 27.342230475763646 22.032396133512655 44-45 22.598300576867295 28.02654176927159 27.354508870254946 22.02064878360617 46-47 22.628618394982112 28.124941916998537 27.297723307265215 21.948716380754142 48-49 22.607650180012044 28.1094048398277 27.27422458438721 22.008720395773047 50-51 22.567817813085984 28.118291790493256 27.298157798289154 22.015732598131606 52-53 22.58056690618655 28.201066353618565 27.261431237571244 21.956935502623644 54-55 22.603353546553098 28.153095326123733 27.23457325538779 22.008977871935382 56-57 22.557180829129567 28.207776826099394 27.219531015216443 22.015511329554602 58-59 22.550892778477568 28.333445308643512 27.18748730220098 21.92817461067794 60-61 22.567664936614598 28.24173954113725 27.197886925320248 21.99270859692791 62-63 22.596731581503075 28.32506124110752 27.131333360421973 21.94687381696744 64-65 22.5885044135035 28.3869158660432 27.145305465293625 21.879274255159682 66-67 22.544053567915572 28.391522275509956 27.13852257764214 21.925901578932336 68-69 22.537005157971684 28.46662485361072 27.055216969932015 21.941153018485583 70-71 22.548470893325614 28.549781607914493 27.034675199855812 21.867072298904077 72-73 22.551926706191942 28.491237965503824 27.03162571655817 21.925209611746062 74-75 22.503774640770466 28.591536999927985 27.031428586371387 21.87325977293016 76-77 22.492843973066385 28.753336629564618 26.890589125574742 21.86323027179425 78-79 22.492421551237555 28.69093486778398 26.91047111298496 21.9061724679935 80-81 22.44491719928695 28.74426461790739 26.90552274299011 21.90529543981555 82-83 22.46246983204106 28.88444027297301 26.86432655701669 21.788763337969243 84-85 22.42754158139445 28.870110115313114 26.880861354316565 21.821486948975867 86-87 22.42573120212804 28.986256002915916 26.82632066161718 21.761692133338858 88-89 22.41218151908522 29.044574353684542 26.809657126236136 21.7335870009941 90-91 22.428285848426196 29.07447377303557 26.71352196412472 21.783718414413517 92-93 22.35882450701661 29.113112460885244 26.74617423597667 21.781888796121475 94-95 22.386894333544625 29.244901207193173 26.645256717432925 21.722947741829273 96-97 22.34765155590029 29.274440220673164 26.653159896317568 21.72474832710898 98-99 22.31991744348368 29.374957682382945 26.57825149459889 21.72687337953448 100-101 22.39336702624294 29.561863168766333 26.243248088319493 21.801521716671232 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 45666.0 1 25921.0 2 4502.5 3 2575.0 4 2225.5 5 2057.5 6 1972.0 7 1977.0 8 2010.0 9 2065.5 10 2155.5 11 2340.0 12 2767.0 13 3590.5 14 4518.0 15 4894.0 16 8119.0 17 9195.0 18 7327.5 19 8090.5 20 10393.5 21 13993.0 22 18430.0 23 24164.5 24 29887.5 25 37838.5 26 49326.5 27 63547.0 28 80041.5 29 103275.0 30 133838.0 31 165605.0 32 199381.0 33 241814.5 34 279461.0 35 317459.5 36 365372.0 37 404407.5 38 430820.5 39 454013.5 40 475744.5 41 480506.5 42 477013.0 43 483848.5 44 488815.5 45 490964.5 46 486366.0 47 477811.0 48 471832.0 49 462136.0 50 445054.5 51 425581.0 52 413047.0 53 395750.5 54 368237.5 55 333835.0 56 297121.0 57 261835.0 58 228955.5 59 194420.5 60 156306.0 61 124169.0 62 99844.0 63 78280.5 64 60883.5 65 46978.0 66 35400.5 67 26298.0 68 20111.0 69 15860.0 70 12707.5 71 10575.5 72 9009.0 73 7442.0 74 5871.0 75 4528.5 76 3361.0 77 2462.5 78 1847.0 79 1258.5 80 760.5 81 505.0 82 323.5 83 204.5 84 130.0 85 78.5 86 36.5 87 9.5 88 6.0 89 5.0 90 2.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0442537154011378 2 0.0010299046493355707 3 1.6896873153161707E-4 4 6.436904058347317E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.046130072934146E-6 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 4.023065036467073E-6 94-95 8.046130072934146E-6 96-97 0.0 98-99 4.023065036467073E-6 100-101 2.413839021880244E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2428335E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.70119285967558 #Duplication Level Percentage of deduplicated Percentage of total 1 78.71599153288233 39.122786763163745 2 11.829816694125673 11.759120020186998 3 3.7824221406535408 5.639726768679858 4 1.7271316343044245 3.4336200980244347 5 0.927732271182239 2.305470026608665 6 0.5512342378056612 1.6438199498421264 7 0.37190352277646915 1.2938834097494232 8 0.25219310326321703 1.0027438450532182 9 0.1917308707711603 0.8576327686815877 >10 1.3055479022452203 13.167804143107235 >50 0.20205628952295862 7.039449130146673 >100 0.1418652127824777 11.88053577491803 >500 1.959451810351418E-4 0.060311908408092446 >1k 1.2992200314704272E-4 0.17942301251169956 >5k 1.624016686627114E-5 0.040938935353848335 >10k+ 3.248033373254228E-5 0.5727334455644748 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60882 0.4898644911003767 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.218452029173658E-5 0.0 0.0 0.0 0.0 2 3.218452029173658E-5 0.0 0.0 0.0 0.0 3 3.218452029173658E-5 0.0 0.0 8.046130072934146E-6 0.0 4 3.218452029173658E-5 0.0 0.0 8.046130072934146E-6 0.0 5 3.218452029173658E-5 0.0 0.0 8.046130072934146E-6 0.0 6 3.218452029173658E-5 0.0 0.0 8.046130072934146E-6 0.0 7 3.218452029173658E-5 0.0 0.0 1.609226014586829E-5 0.0 8 3.218452029173658E-5 0.0 0.0 2.413839021880244E-5 0.0 9 3.218452029173658E-5 0.0 0.0 2.413839021880244E-5 0.0 10-11 4.023065036467073E-5 8.046130072934146E-6 0.0 2.816145525526951E-5 0.0 12-13 4.42537154011378E-5 8.046130072934146E-6 4.023065036467073E-6 4.023065036467073E-5 4.023065036467073E-6 14-15 5.229984547407195E-5 8.046130072934146E-6 8.046130072934146E-6 5.229984547407195E-5 2.413839021880244E-5 16-17 7.643823569287439E-5 8.046130072934146E-6 8.046130072934146E-6 6.03459755470061E-5 2.413839021880244E-5 18-19 1.0057662591167682E-4 8.046130072934146E-6 1.206919510940122E-5 6.436904058347317E-5 2.413839021880244E-5 20-21 1.1666888605754513E-4 1.609226014586829E-5 1.609226014586829E-5 7.241517065640732E-5 3.218452029173658E-5 22-23 1.327611462034134E-4 1.609226014586829E-5 1.609226014586829E-5 7.241517065640732E-5 4.023065036467073E-5 24-25 1.5287647138574877E-4 1.609226014586829E-5 1.609226014586829E-5 8.046130072934145E-5 4.023065036467073E-5 26-27 1.7299179656808414E-4 1.609226014586829E-5 1.609226014586829E-5 8.046130072934145E-5 4.023065036467073E-5 28-29 1.931071217504195E-4 1.609226014586829E-5 1.609226014586829E-5 8.046130072934145E-5 4.023065036467073E-5 30-31 2.091993818962878E-4 2.413839021880244E-5 1.609226014586829E-5 8.046130072934145E-5 4.827678043760488E-5 32-33 2.252916420421561E-4 2.413839021880244E-5 2.0115325182335364E-5 9.253049583874269E-5 5.632291051053902E-5 34-35 2.252916420421561E-4 3.218452029173658E-5 2.413839021880244E-5 9.655356087520976E-5 6.839210561994025E-5 36-37 2.5747616233389267E-4 3.218452029173658E-5 2.413839021880244E-5 9.655356087520976E-5 8.046130072934145E-5 38-39 2.73568422479761E-4 3.218452029173658E-5 2.413839021880244E-5 9.655356087520976E-5 8.046130072934145E-5 40-41 3.178221378808988E-4 3.218452029173658E-5 2.413839021880244E-5 1.1264582102107804E-4 8.046130072934145E-5 42-43 3.580527882455695E-4 3.620758532820366E-5 2.413839021880244E-5 1.1666888605754513E-4 8.046130072934145E-5 44-45 3.902373085373061E-4 4.023065036467073E-5 2.413839021880244E-5 1.206919510940122E-4 8.046130072934145E-5 46-47 4.3449102393844385E-4 4.023065036467073E-5 3.218452029173658E-5 1.206919510940122E-4 9.655356087520976E-5 48-49 4.908139344489829E-4 4.023065036467073E-5 3.218452029173658E-5 1.2471501613047926E-4 9.655356087520976E-5 50-51 5.632291051053903E-4 4.023065036467073E-5 3.620758532820366E-5 1.2873808116694633E-4 9.655356087520976E-5 52-53 8.086360723298816E-4 4.023065036467073E-5 4.023065036467073E-5 1.2873808116694633E-4 1.1264582102107804E-4 54-55 0.0013839343725446731 4.023065036467073E-5 4.023065036467073E-5 1.2873808116694633E-4 1.206919510940122E-4 56-57 0.0021442936644369497 4.023065036467073E-5 4.023065036467073E-5 1.2873808116694633E-4 1.206919510940122E-4 58-59 0.003166152183699586 4.023065036467073E-5 4.023065036467073E-5 1.367842112398805E-4 1.206919510940122E-4 60-61 0.004256402808582163 4.023065036467073E-5 4.023065036467073E-5 1.5287647138574877E-4 1.2873808116694633E-4 62-63 0.005624244920980968 4.827678043760488E-5 4.023065036467073E-5 1.5287647138574877E-4 1.3678421123988047E-4 64-65 0.007639800504250971 5.632291051053902E-5 4.023065036467073E-5 1.5689953642221584E-4 1.3678421123988047E-4 66-67 0.009892716924672532 5.632291051053902E-5 4.023065036467073E-5 1.609226014586829E-4 1.3678421123988047E-4 68-69 0.01235483272699038 6.839210561994025E-5 4.023065036467073E-5 1.6494566649514998E-4 1.3678421123988047E-4 70-71 0.015681907512148652 7.241517065640732E-5 4.023065036467073E-5 1.770148616045512E-4 1.4483034131281463E-4 72-73 0.020207855678174107 8.046130072934145E-5 4.023065036467073E-5 1.770148616045512E-4 1.5287647138574877E-4 74-75 0.02604532304608783 8.046130072934145E-5 4.023065036467073E-5 1.8506099167748535E-4 1.5287647138574877E-4 76-77 0.032860395217863055 8.046130072934145E-5 4.023065036467073E-5 1.8506099167748535E-4 1.5287647138574877E-4 78-79 0.0412364166237875 8.046130072934145E-5 4.023065036467073E-5 1.8506099167748535E-4 1.5287647138574877E-4 80-81 0.051499255531815 8.046130072934145E-5 4.023065036467073E-5 1.8506099167748535E-4 1.5287647138574877E-4 82-83 0.06481560080252102 8.046130072934145E-5 4.023065036467073E-5 1.931071217504195E-4 1.5287647138574877E-4 84-85 0.081129129525395 8.046130072934145E-5 4.023065036467073E-5 1.931071217504195E-4 1.5287647138574877E-4 86-87 0.10223010564166479 8.046130072934145E-5 4.023065036467073E-5 1.931071217504195E-4 1.5689953642221584E-4 88-89 0.1282191057772421 8.046130072934145E-5 4.023065036467073E-5 2.0517631685982072E-4 1.7299179656808414E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 7445 0.0 87.82713 1 TCGACGG 7625 0.0 85.84496 2 CGACGGC 8335 0.0 78.75849 3 ACGGCGC 8990 0.0 72.86115 5 GACGGCG 9000 0.0 72.78049 4 CGGCGCG 10030 0.0 65.2589 6 CGCGCCG 14010 0.0 49.093266 9 GGCGCGC 14625 0.0 47.15874 7 GCGCGCC 14785 0.0 46.648403 8 TATCTCG 2045 0.0 29.267302 2 GCCGGAT 13990 0.0 25.854948 12-13 CGCCGGA 13685 0.0 25.702284 10-11 ATCTCGA 4525 0.0 25.623957 1 TCGAGGG 4670 0.0 24.716251 4 CCGGATC 14410 0.0 24.508032 12-13 GCGCCGG 15070 0.0 24.45907 10-11 GGGCGCG 4955 0.0 23.198675 8 CTCGAGG 5010 0.0 22.944185 3 CGGATCC 16295 0.0 22.401701 14-15 TCCATAT 15480 0.0 21.540592 18-19 >>END_MODULE