FastQCFastQC Report
Tue 31 May 2016
SRR1051285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21200161
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT4983932.350892523882248No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT868400.40961953071960167No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA338600.15971576819628872No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA228900.10797087814568955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG2007600.094.280861
TCGACGG2033800.093.103692
CGACGGC2059400.091.849293
ACGGCGC2078050.090.9656755
GACGGCG2082600.090.812764
CGGCGCG2085500.090.60796
TATCTCG926150.088.946862
ATATCTC939200.087.903171
ATCTCGA987800.083.275823
TCTCGAG1006800.080.702314
CTCGAGG1031850.078.724515
TCGAGGG1041550.077.9703456
CGAGGGC1054650.076.770717
AGGGCGC1061850.076.437869
GAGGGCG1087050.074.6789868
CGCGCCG3125050.061.0662169
GGCGCGC3141650.060.8192837
GCGCGCC3143300.060.766068
TCCATAT2251800.041.90559418-19
ATCCATA2280900.041.57295216-17
Produced by FastQC (version 0.11.5)