##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051285_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21200161 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.129086142317504 33.0 31.0 34.0 30.0 34.0 2 32.37607969109291 34.0 31.0 34.0 31.0 34.0 3 32.44017962882452 34.0 31.0 34.0 31.0 34.0 4 36.0138606494545 37.0 35.0 37.0 35.0 37.0 5 35.95228847554507 37.0 35.0 37.0 35.0 37.0 6 36.0325706960433 37.0 37.0 37.0 35.0 37.0 7 35.982160512837616 37.0 36.0 37.0 35.0 37.0 8 35.994602824006854 37.0 36.0 37.0 35.0 37.0 9 37.68052714316651 39.0 38.0 39.0 35.0 39.0 10-11 37.67546788913537 39.0 38.0 39.0 35.0 39.0 12-13 37.65285313163423 39.0 38.0 39.0 35.0 39.0 14-15 39.09641650834632 41.0 39.0 41.0 36.0 41.0 16-17 38.98715247964391 40.5 38.5 41.0 35.5 41.0 18-19 39.01654673754601 40.5 39.0 41.0 36.0 41.0 20-21 39.05207387340124 41.0 39.0 41.0 36.0 41.0 22-23 38.95319967145532 40.5 39.0 41.0 35.5 41.0 24-25 38.92204389391194 40.5 39.0 41.0 35.0 41.0 26-27 38.90534435092262 40.0 39.0 41.0 35.0 41.0 28-29 38.69520559301413 40.0 38.0 41.0 34.5 41.0 30-31 38.20466993151609 40.0 36.5 41.0 34.0 41.0 32-33 37.89033411114189 40.0 35.5 41.0 34.0 41.0 34-35 37.645078591620134 39.5 35.0 41.0 33.5 41.0 36-37 37.496036681042185 39.0 35.0 41.0 33.0 41.0 38-39 37.18070060883028 39.0 35.0 41.0 32.0 41.0 40-41 36.164142833632255 39.0 35.0 41.0 27.0 41.0 42-43 36.31038276077243 39.0 35.0 41.0 28.0 41.0 44-45 36.20673439696991 39.0 35.0 41.0 28.0 41.0 46-47 36.24123913492922 39.0 35.0 41.0 28.0 41.0 48-49 36.20370033982289 39.0 35.0 41.0 27.5 41.0 50-51 36.07951076409279 39.0 35.0 40.5 27.5 41.0 52-53 36.149544619024354 39.0 35.0 40.5 28.0 41.0 54-55 36.01249452775382 39.0 35.0 41.0 28.0 41.0 56-57 35.68021964550175 39.0 35.0 41.0 27.0 41.0 58-59 35.374614442786545 39.0 34.0 41.0 26.0 41.0 60-61 35.05662730580207 38.0 34.0 40.5 26.0 41.0 62-63 34.76602769667646 38.0 34.0 40.0 25.0 41.0 64-65 34.43670413635066 37.0 34.0 40.0 25.0 41.0 66-67 34.069709777204054 37.0 34.0 40.0 25.0 41.0 68-69 33.6861901426126 36.0 34.0 39.0 24.5 41.0 70-71 33.28820658484622 36.0 33.0 39.0 23.5 41.0 72-73 32.88084354642401 35.0 33.0 38.5 22.5 40.0 74-75 32.46506028892894 35.0 33.0 37.0 20.5 39.5 76-77 32.06262058575876 35.0 33.0 37.0 20.0 39.0 78-79 31.70530612951477 35.0 32.0 36.5 19.0 39.0 80-81 31.3809076732955 35.0 32.0 36.0 18.5 37.0 82-83 31.098640901830887 35.0 32.0 35.5 18.0 37.0 84-85 30.837914273386886 35.0 32.0 35.0 17.5 37.0 86-87 30.61436771635838 35.0 32.0 35.0 13.5 36.0 88-89 30.417709233434596 35.0 32.0 35.0 8.5 36.0 90-91 30.198600968171892 35.0 32.0 35.0 6.5 36.0 92-93 29.98897480071024 35.0 31.0 35.0 2.0 35.0 94-95 29.774750012511696 35.0 31.0 35.0 2.0 35.0 96-97 29.532371240010868 35.0 31.0 35.0 2.0 35.0 98-99 29.25580973182232 35.0 31.0 35.0 2.0 35.0 100-101 28.21064099466037 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30869749788119805 1101 2 0.30957051043436223 1101 3 0.29570007657986963 1101 4 0.24611451506198279 1101 5 0.2420652435843138 1101 6 0.25043337126973597 1101 7 0.2642325998604633 1101 8 0.2284940624350824 1101 9 0.32228009149163483 1101 10-11 0.32297805151029735 1101 12-13 0.33975579239873355 1101 14-15 0.4161337261891944 1101 16-17 0.3538150647399476 1101 18-19 0.38086276231270233 1101 20-21 0.38717015571536706 1101 22-23 0.4582705056381968 1101 24-25 0.4288709781976081 1101 26-27 0.35354134359891276 1101 28-29 0.3439327735930817 1101 30-31 0.37206181293426255 1101 32-33 0.37199182899328775 1101 34-35 0.3534922330451451 1101 36-37 0.3179792968083035 1101 38-39 0.3155354997324409 1101 40-41 0.24426502960258034 1101 42-43 0.23888229109928716 1101 44-45 0.20891711597835183 1101 46-47 0.23918758772701665 1101 48-49 0.19010545397886602 1101 50-51 0.18230393883874285 1101 52-53 0.12924047487208412 1101 54-55 0.1374471732743956 1101 56-57 0.1567805080195228 1101 58-59 0.11633268455210555 1101 60-61 0.05263580090475983 1101 62-63 0.027006830381431257 1101 64-65 0.022454777307714835 1101 66-67 0.04710676901546407 1101 68-69 -0.018046707542701768 1101 70-71 -0.05118763106654001 1101 72-73 -0.08222611446532113 1101 74-75 -0.11802962563309904 1101 76-77 -0.12409506985024876 1101 78-79 -0.16993966241124525 1101 80-81 -0.1907384048402747 1101 82-83 -0.19045614005515432 1101 84-85 -0.22044229724173903 1101 86-87 -0.22293801853573214 1101 88-89 -0.2072947677762329 1101 90-91 -0.25745783275026923 1101 92-93 -0.2805062602160149 1101 94-95 -0.314839606007304 1101 96-97 -0.3043233066797093 1101 98-99 -0.34217626009931124 1101 100-101 -0.34197208572421545 1102 1 0.24325049568771107 1102 2 0.240312845805434 1102 3 0.2639622081964319 1102 4 0.16619985541700544 1102 5 0.2277326469374117 1102 6 0.2083548884457187 1102 7 0.23045053979013375 1102 8 0.23303854484228026 1102 9 0.2506820730501431 1102 10-11 0.2781401876410641 1102 12-13 0.2700491558931617 1102 14-15 0.3669007270661595 1102 16-17 0.3629077804695555 1102 18-19 0.3277839761422996 1102 20-21 0.3371347128397417 1102 22-23 0.3604451241545945 1102 24-25 0.33404227711920953 1102 26-27 0.3109023851783448 1102 28-29 0.29281373685707024 1102 30-31 0.2737636907777983 1102 32-33 0.26218071341163807 1102 34-35 0.3129859171543785 1102 36-37 0.25653722047323413 1102 38-39 0.281149734523936 1102 40-41 0.24092249475747707 1102 42-43 0.19753636076360692 1102 44-45 0.1698936665461801 1102 46-47 0.12749583874740011 1102 48-49 0.1538591470738595 1102 50-51 0.13856749020388293 1102 52-53 0.14202748970223666 1102 54-55 0.2030557740324639 1102 56-57 0.16673057460488394 1102 58-59 0.13303804080192805 1102 60-61 0.15689423737538277 1102 62-63 0.13038511383030738 1102 64-65 0.08951633748704069 1102 66-67 0.05808731633457853 1102 68-69 0.02833121135844152 1102 70-71 0.06403050471021743 1102 72-73 2.9688259365201475E-4 1102 74-75 -0.006919639007278988 1102 76-77 -0.03531823020983893 1102 78-79 -0.03828054931897995 1102 80-81 -0.034460773266481226 1102 82-83 -0.03795107259377062 1102 84-85 -0.058743158555220276 1102 86-87 -0.08063511871892004 1102 88-89 -0.07488467911520758 1102 90-91 -0.14012540648935712 1102 92-93 -0.1564748135902505 1102 94-95 -0.19026823512328406 1102 96-97 -0.20063341173583993 1102 98-99 -0.22031850993057134 1102 100-101 -0.26385866208728004 1103 1 0.13878205519820597 1103 2 0.12333005341947256 1103 3 0.0957719103402539 1103 4 0.123523716760495 1103 5 0.13347657786119527 1103 6 0.15030180763594814 1103 7 0.16303194545736943 1103 8 0.19613900305569842 1103 9 0.22562690835722066 1103 10-11 0.1947612568185022 1103 12-13 0.18793426540567282 1103 14-15 0.25271291960655873 1103 16-17 0.2827044587364327 1103 18-19 0.3081405433152824 1103 20-21 0.27303573528472214 1103 22-23 0.32173807468109317 1103 24-25 0.2977241856831938 1103 26-27 0.2676620651375998 1103 28-29 0.25751449916139535 1103 30-31 0.24078034255585123 1103 32-33 0.22523054944481657 1103 34-35 0.16593860276118022 1103 36-37 0.1832808275302682 1103 38-39 0.18739520946567723 1103 40-41 0.04613774876815313 1103 42-43 -0.022160589495484828 1103 44-45 -0.04906651739198509 1103 46-47 -0.03199280915995928 1103 48-49 0.009355718327689999 1103 50-51 -0.08427238792587133 1103 52-53 -0.07179733564883861 1103 54-55 -0.06122547129848499 1103 56-57 -0.05153999176310009 1103 58-59 -0.09194146116413293 1103 60-61 -0.09598972363633607 1103 62-63 -0.10017054063361996 1103 64-65 -0.13710315773812454 1103 66-67 -0.13652230728180825 1103 68-69 -0.20609709225699646 1103 70-71 -0.310518889813423 1103 72-73 -0.3054098398713023 1103 74-75 -0.36210910193129564 1103 76-77 -0.3316509977581674 1103 78-79 -0.35920876705867144 1103 80-81 -0.39587883270855073 1103 82-83 -0.4270111769746876 1103 84-85 -0.4032116304626818 1103 86-87 -0.4194934761917146 1103 88-89 -0.4506768806725603 1103 90-91 -0.4684744645092458 1103 92-93 -0.5126081984315753 1103 94-95 -0.6027487363548687 1103 96-97 -0.6267572363552283 1103 98-99 -0.714912156207685 1103 100-101 -0.7700166947507867 1104 1 0.06761997437052969 1104 2 0.10606004289557092 1104 3 0.0910560211106457 1104 4 0.10868245609139393 1104 5 0.1401927221653736 1104 6 0.14166820803578872 1104 7 0.15823214418612253 1104 8 0.1669799581259781 1104 9 0.2110400476084422 1104 10-11 0.19940137706491612 1104 12-13 0.208263954925485 1104 14-15 0.22149114887583465 1104 16-17 0.2959640390840619 1104 18-19 0.2619719919677834 1104 20-21 0.2696765633017435 1104 22-23 0.29226581332798673 1104 24-25 0.30288400545082794 1104 26-27 0.2279337207167771 1104 28-29 0.17428051196989003 1104 30-31 0.17561785584332767 1104 32-33 0.16511062162952328 1104 34-35 0.16165426164791086 1104 36-37 0.11853885581509616 1104 38-39 0.09455314597803266 1104 40-41 0.04867307696100909 1104 42-43 -0.07108799826936263 1104 44-45 -0.03163784527956892 1104 46-47 -0.04364253213186231 1104 48-49 -0.11366617074384777 1104 50-51 -0.13652962300636773 1104 52-53 -0.11198014726947036 1104 54-55 -0.10144394992950367 1104 56-57 -0.06638500528397628 1104 58-59 -0.13501836879969176 1104 60-61 -0.1628170940794007 1104 62-63 -0.13615421612625767 1104 64-65 -0.17932388224538443 1104 66-67 -0.21387759390287897 1104 68-69 -0.3170725931964 1104 70-71 -0.3765181646709692 1104 72-73 -0.3948869628582159 1104 74-75 -0.43502855091320214 1104 76-77 -0.41208332114339186 1104 78-79 -0.45318796037986786 1104 80-81 -0.4766415777843598 1104 82-83 -0.5174185671023288 1104 84-85 -0.5548032457298682 1104 86-87 -0.5630103592838829 1104 88-89 -0.6203175820805598 1104 90-91 -0.6660042691806005 1104 92-93 -0.7442589902368866 1104 94-95 -0.7917995650521661 1104 96-97 -0.8360146481048645 1104 98-99 -0.873831164826246 1104 100-101 -0.9356086567257762 1105 1 0.02568620791270604 1105 2 0.01223380935769569 1105 3 0.07238146732762374 1105 4 0.08424700612265923 1105 5 0.10677760411127224 1105 6 0.11096009389716244 1105 7 0.13731810341253947 1105 8 0.11483044184573998 1105 9 0.11760750405530018 1105 10-11 0.11618297781705422 1105 12-13 0.14536074929842613 1105 14-15 0.1918045087705451 1105 16-17 0.2317114661086066 1105 18-19 0.20540530337694918 1105 20-21 0.1838667842203634 1105 22-23 0.2142150689989677 1105 24-25 0.15442319969493212 1105 26-27 0.07819538779233426 1105 28-29 0.07102526422436739 1105 30-31 0.11691965531660742 1105 32-33 0.09106934759637397 1105 34-35 -0.0020165342047917534 1105 36-37 0.0317605471785356 1105 38-39 0.04624695581811267 1105 40-41 -0.029773435396670322 1105 42-43 -0.09123920093325921 1105 44-45 -0.15180776107798266 1105 46-47 -0.18601832591645007 1105 48-49 -0.18877067031033334 1105 50-51 -0.1671927343385562 1105 52-53 -0.14900392014560992 1105 54-55 -0.14400312706277418 1105 56-57 -0.111143931516267 1105 58-59 -0.13379130265888506 1105 60-61 -0.21799125429594568 1105 62-63 -0.2939685311425748 1105 64-65 -0.27250638932537186 1105 66-67 -0.3372966387619414 1105 68-69 -0.3606616397678408 1105 70-71 -0.3825418421289086 1105 72-73 -0.3692222962148577 1105 74-75 -0.3899855582249643 1105 76-77 -0.45320946683961694 1105 78-79 -0.5272185515490015 1105 80-81 -0.533677541851489 1105 82-83 -0.5698010766738157 1105 84-85 -0.5986719959389468 1105 86-87 -0.6511461595077215 1105 88-89 -0.6850412803672654 1105 90-91 -0.7459129807649099 1105 92-93 -0.8192666486610207 1105 94-95 -0.8400946105605236 1105 96-97 -0.8672482039714673 1105 98-99 -0.9770628533529155 1105 100-101 -1.046041752759379 1106 1 -0.006868264014833869 1106 2 -1.4203570484028205E-4 1106 3 -0.009270369184250171 1106 4 0.04312678179084628 1106 5 0.015574244485186739 1106 6 0.05040751179215874 1106 7 0.033673623402407316 1106 8 0.04711420874796346 1106 9 0.01928794995063754 1106 10-11 0.0642459268563016 1106 12-13 0.03843943521587079 1106 14-15 0.0881862463785339 1106 16-17 0.1064037944254963 1106 18-19 0.06172073472521333 1106 20-21 0.02218465552404325 1106 22-23 0.06950832631005 1106 24-25 0.03127951594287737 1106 26-27 -3.466477680973412E-4 1106 28-29 0.008225365434114451 1106 30-31 -0.03669541966858958 1106 32-33 -0.012537481049172072 1106 34-35 -0.046866250667278564 1106 36-37 -0.05464623566275861 1106 38-39 -0.021995706288521433 1106 40-41 -0.08401765371259273 1106 42-43 -0.18313263051518902 1106 44-45 -0.18577218694647968 1106 46-47 -0.17924943563885165 1106 48-49 -0.20209099837835964 1106 50-51 -0.27671783630421487 1106 52-53 -0.22606918088430206 1106 54-55 -0.22527101824088902 1106 56-57 -0.2449871182593526 1106 58-59 -0.31332386574657534 1106 60-61 -0.318778913210771 1106 62-63 -0.35531788368309236 1106 64-65 -0.2911867314519867 1106 66-67 -0.30928461325071055 1106 68-69 -0.3510515885782013 1106 70-71 -0.4055386747818517 1106 72-73 -0.4142574048800256 1106 74-75 -0.42272444993196956 1106 76-77 -0.5226267769642945 1106 78-79 -0.5225609100839748 1106 80-81 -0.5306148581968486 1106 82-83 -0.5600670068962117 1106 84-85 -0.6065965938897406 1106 86-87 -0.65699341625184 1106 88-89 -0.6554241968475871 1106 90-91 -0.707656520884548 1106 92-93 -0.7516515443971272 1106 94-95 -0.8251705693396865 1106 96-97 -0.9021790443103832 1106 98-99 -0.9404697184297 1106 100-101 -0.96907620073506 1107 1 -0.0886027222900907 1107 2 -0.07639933319650538 1107 3 -0.08352841418753343 1107 4 -0.05995542371421436 1107 5 -0.1433269275690563 1107 6 -0.09356160668417601 1107 7 -0.02581918073357059 1107 8 -0.06017736041542321 1107 9 -0.0638260332202023 1107 10-11 -0.03944312468529887 1107 12-13 -0.06550125126376827 1107 14-15 -0.08819107204735843 1107 16-17 -0.03823806822550324 1107 18-19 -0.009532920005881351 1107 20-21 -0.03085672843783982 1107 22-23 -0.040356975607465984 1107 24-25 -0.049519292520052716 1107 26-27 -0.11667904310397148 1107 28-29 -0.15235122131301182 1107 30-31 -0.12981569324445275 1107 32-33 -0.11014574847699521 1107 34-35 -0.12184677909858976 1107 36-37 -0.1474991136609134 1107 38-39 -0.24751462709652117 1107 40-41 -0.22763029054628703 1107 42-43 -0.19799813247553288 1107 44-45 -0.1454169288265703 1107 46-47 -0.270780577643265 1107 48-49 -0.1905172822479031 1107 50-51 -0.20223175379024383 1107 52-53 -0.19637943985369333 1107 54-55 -0.11330458394030529 1107 56-57 -0.10905892097287762 1107 58-59 -0.22462154189847183 1107 60-61 -0.2517943026211711 1107 62-63 -0.3074972429844607 1107 64-65 -0.23954026391210448 1107 66-67 -0.24524181416619228 1107 68-69 -0.21725200126046218 1107 70-71 -0.23867868093416433 1107 72-73 -0.2587061210555035 1107 74-75 -0.26175212609858534 1107 76-77 -0.2722331360441146 1107 78-79 -0.32818299228171455 1107 80-81 -0.34455606009763784 1107 82-83 -0.37657235917223986 1107 84-85 -0.36349863117460046 1107 86-87 -0.38430869510596466 1107 88-89 -0.47497000556842295 1107 90-91 -0.48042535648730933 1107 92-93 -0.5052399834101422 1107 94-95 -0.5826753957256514 1107 96-97 -0.5828026314308907 1107 98-99 -0.6019477539144056 1107 100-101 -0.6801877487276684 1108 1 -0.3151090657435809 1108 2 -0.24130858341573713 1108 3 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-0.6008321909558916 2308 30-31 -0.5792158230703137 2308 32-33 -0.4885568522909125 2308 34-35 -0.5150229235932784 2308 36-37 -0.4681219783457635 2308 38-39 -0.48129060632275866 2308 40-41 -0.48764506980729294 2308 42-43 -0.6140652097250978 2308 44-45 -0.5590264840389807 2308 46-47 -0.4977977028992342 2308 48-49 -0.2798876914120072 2308 50-51 -0.9378302187872904 2308 52-53 -1.0388018469256224 2308 54-55 -0.752008826416116 2308 56-57 -0.8721599669656399 2308 58-59 -1.1851379318018402 2308 60-61 -1.2237651519539199 2308 62-63 -1.200573534557826 2308 64-65 -1.279481480822973 2308 66-67 -1.0495023733535476 2308 68-69 -0.9344557870359083 2308 70-71 -0.8400242732061827 2308 72-73 -0.8497278017251233 2308 74-75 -1.0629372626674964 2308 76-77 -0.8981018974529675 2308 78-79 -0.829713132571694 2308 80-81 -0.8677751246711338 2308 82-83 -0.8390568397922209 2308 84-85 -0.8170054335883457 2308 86-87 -0.7614090463218197 2308 88-89 -0.8571088592681946 2308 90-91 -0.8961635533459678 2308 92-93 -0.8464411915711665 2308 94-95 -0.8241282306004294 2308 96-97 -0.8486716430767594 2308 98-99 -0.8789310650913542 2308 100-101 -0.4002879515938105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 182.0 11 1758.0 12 6510.0 13 13737.0 14 21116.0 15 28177.0 16 38072.0 17 58673.0 18 123980.0 19 316988.0 20 348021.0 21 251619.0 22 213107.0 23 198057.0 24 199174.0 25 219844.0 26 257224.0 27 301214.0 28 343920.0 29 384112.0 30 428253.0 31 486886.0 32 571773.0 33 696915.0 34 910325.0 35 1345189.0 36 2349075.0 37 4543152.0 38 5463667.0 39 1078766.0 40 675.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.80952036161359 30.226722648778882 21.45051193009843 19.5132450595091 2 21.13887070848174 25.271027492455193 35.953909688039936 17.636192111023128 3 20.575828220708413 27.487751823370022 22.948216376230324 28.98820357969124 4 30.54808742367745 22.983072536655275 26.540650776012477 19.9281892636548 5 21.27078248827044 32.837651117655405 22.23756376364187 23.654002630432284 6 21.28346522582574 22.88672717466479 25.38976941431922 30.44003818519025 7 30.726992067226337 22.913152225056336 22.10757770332818 24.252278004389147 8 34.00134555581913 22.969849144070178 22.298005189677568 20.730800110433123 9 20.71706908263574 26.436629419936953 22.55402683026794 30.29227466715937 10-11 29.46940355783147 22.77509590611128 22.470081713058686 25.28541882299856 12-13 27.25304303113547 22.70979687371242 22.697146026391028 27.340014068761082 14-15 27.14357923979917 22.768713879106862 22.741780121386814 27.345926759707158 16-17 27.075242494620678 27.50407178511522 22.712721379804616 22.70796434045949 18-19 22.406289744686372 22.82643042191991 27.539281423381645 27.227998410012077 20-21 22.11408441662306 29.523971539650102 23.139260593351153 25.222683450375683 22-23 20.080394672474423 27.365624723321673 29.977600170111916 22.576380434091988 24-25 20.070123524061916 22.727716548945075 34.88599449787198 22.316165429121035 26-27 20.04296335296699 22.74090512803181 37.027690497256124 20.18844102174507 28-29 20.031321931941932 22.64955204821322 37.19934249555935 20.119783524285502 30-31 20.047258131671736 22.598403851744333 37.18152895159617 20.172809064987764 32-33 20.069191764091872 22.630712031021226 37.162248995775556 20.13784720911135 34-35 20.065783934376725 22.698525732894197 37.14385706787793 20.09183326485115 36-37 20.103484119766826 22.68914844561794 37.030975849664536 20.176391584950697 38-39 20.343315574311006 22.85237967565838 36.20545047961058 20.59885427042003 40-41 21.733620324864514 24.051423477397176 31.991351386435223 22.223604811303083 42-43 21.78803859044467 24.42906863940407 32.2461240016182 21.53676876853306 44-45 22.065944026499327 25.24646421826931 31.2565154513174 21.43107630391396 46-47 21.700313974030667 25.97597725790856 31.012575800721514 21.311132967339258 48-49 21.658526555529463 26.434947828933943 30.387790451214023 21.51873516432257 50-51 21.72255998871705 26.598425740971194 29.984548445628818 21.694465824682936 52-53 21.772425196632373 26.588285965518494 29.852040550034086 21.787248287815046 54-55 21.93102103454651 26.372257056045562 29.698368646884482 21.998353262523448 56-57 22.000509102728962 26.275613538245807 29.676236277534052 22.047641081491175 58-59 22.004864170694887 26.31314884805248 29.619708039853265 22.062278941399363 60-61 22.066407216344462 26.303410271960097 29.46737824819688 22.162804263498565 62-63 22.216861713828933 26.41982158398864 29.14723567544729 22.21608102673514 64-65 22.311621183644274 26.564892463620932 28.849822653594675 22.27366369914012 66-67 22.39393911091161 26.61020403850461 28.56480100410711 22.43105584647667 68-69 22.415103356998088 26.688733637447378 28.402291378824906 22.49387162672963 70-71 22.475251485118438 26.845234807414908 28.205344761296857 22.474168946169794 72-73 22.537460918339253 26.853126256918518 28.05916426766759 22.55024855707464 74-75 22.55822771438475 26.850735179957102 28.032469771026854 22.558567334631295 76-77 22.617931905860843 26.90546732795055 27.909706905534783 22.566893860653824 78-79 22.64970933499571 26.82238786471573 27.853763533782285 22.674139266506284 80-81 22.64084778693615 26.87128688071222 27.77493006058731 22.712935271764323 82-83 22.702597778588864 26.905952950236124 27.73148720863658 22.659962062538437 84-85 22.705174628093484 26.850341361691505 27.687452226356452 22.757031783858565 86-87 22.74957311295217 26.89408330860617 27.650764023981573 22.70557955446009 88-89 22.784787467097196 26.926732956473792 27.550864410424538 22.73761516600447 90-91 22.82553168245544 26.884558548278026 27.42051225592305 22.869397513343475 92-93 22.789061191381744 26.88916155646877 27.440299235792292 22.881478016357192 94-95 22.81971448933141 26.970816508474808 27.358485871340505 22.850983130853276 96-97 22.815740541580816 26.930683542011003 27.35925813767443 22.894317778733743 98-99 22.877793959347585 26.942849105812016 27.317055867500656 22.86230106733974 100-101 23.034529980228644 26.946544413547596 27.089142706712394 22.929782899511363 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 17323.0 1 9673.5 2 1565.0 3 897.5 4 630.5 5 547.5 6 501.0 7 450.5 8 433.0 9 439.0 10 463.5 11 536.0 12 780.0 13 1436.0 14 2208.0 15 4769.5 16 85413.5 17 97451.0 18 30033.5 19 27697.0 20 26669.0 21 28811.0 22 33820.0 23 41528.0 24 51919.5 25 64832.0 26 81383.5 27 102681.5 28 128113.5 29 159529.5 30 198372.5 31 242862.5 32 291427.5 33 346171.0 34 403053.5 35 461778.5 36 526929.0 37 589427.5 38 642323.0 39 688743.0 40 727324.5 41 751854.0 42 770678.0 43 796710.0 44 824385.0 45 847647.5 46 853877.0 47 847701.5 48 841130.0 49 827984.5 50 803283.0 51 772825.0 52 746879.0 53 723325.0 54 682654.5 55 626709.5 56 569758.5 57 506535.0 58 444301.0 59 381482.5 60 315909.0 61 260026.0 62 212661.5 63 166689.0 64 128175.5 65 97793.5 66 72021.0 67 52489.0 68 38957.0 69 29535.5 70 22871.5 71 18116.0 72 14900.5 73 11519.5 74 8244.0 75 6105.0 76 4477.5 77 3095.5 78 2017.5 79 1211.5 80 617.0 81 311.0 82 158.5 83 86.0 84 49.0 85 21.5 86 9.5 87 7.5 88 5.5 89 4.5 90 5.5 91 6.5 92 4.5 93 5.0 94 5.5 95 3.5 96 4.5 97 5.5 98 4.5 99 4.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001386781921137297 2 0.010032942674350445 3 0.003202805865483757 4 0.0038867629354324244 5 0.00643391340282746 6 0.0015047055538870673 7 6.415045621587497E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 4.457513317941312E-4 34-35 0.0 36-37 0.0 38-39 2.1226253894958628E-4 40-41 0.0 42-43 2.0047017567460926E-4 44-45 3.5612937090430586E-4 46-47 0.0 48-49 0.0 50-51 1.3443294133473797E-4 52-53 6.108444176438094E-4 54-55 2.853751912544438E-4 56-57 5.542410739239197E-4 58-59 1.9575323036461847E-4 60-61 0.0016108368233618605 62-63 0.00418157201730685 64-65 5.943351090588416E-4 66-67 0.003910347661982378 68-69 0.0 70-71 0.0 72-73 0.0 74-75 5.424487106489427E-5 76-77 0.0013655556672423383 78-79 0.0035683691270080448 80-81 0.004337231212536546 82-83 0.003766480830027659 84-85 0.002733469807139672 86-87 0.002200454987110711 88-89 0.0010377279681979773 90-91 0.001363197194587343 92-93 0.00263205548297487 94-95 3.5377089824931046E-5 96-97 4.716945309990806E-6 98-99 5.495241286139289E-4 100-101 2.240549022245633E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.1200161E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.64671705038563 #Duplication Level Percentage of deduplicated Percentage of total 1 75.11922927752784 41.05019267372108 2 13.642488971598809 14.910344693879333 3 4.888737952222806 8.014604388258041 4 2.3043079156174104 5.0369145064683405 5 1.245434201499651 3.4029445207112192 6 0.749456220279968 2.4573193206774544 7 0.4759794601728518 1.820750041730279 8 0.30709270390239307 1.3425286478713545 9 0.2278206060521611 1.120468337746184 >10 0.9070731843099056 8.616016047103878 >50 0.07661771375148334 2.9589291055095894 >100 0.05423072746203135 4.579792214619107 >500 7.027148642102048E-4 0.25825719975465244 >1k 6.406767726375144E-4 0.628580387093603 >5k 1.023676176762684E-4 0.36611220304772074 >10k+ 8.530634806348995E-5 3.4362457118081737 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 498393 2.350892523882248 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 86840 0.40961953071960167 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 33860 0.15971576819628872 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 22890 0.10797087814568955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.6886588266947594E-4 4.716945309990806E-6 0.0 4.716945309990806E-6 0.0 2 2.877336639094392E-4 4.716945309990806E-6 0.0 9.433890619981612E-6 4.716945309990806E-6 3 3.066014451494024E-4 4.716945309990806E-6 0.0 1.4150835929972419E-5 4.716945309990806E-6 4 3.16035335769384E-4 4.716945309990806E-6 0.0 1.8867781239963225E-5 4.716945309990806E-6 5 3.2546922638936564E-4 9.433890619981612E-6 0.0 2.358472654995403E-5 4.716945309990806E-6 6 3.3490311700934725E-4 9.433890619981612E-6 0.0 2.358472654995403E-5 1.8867781239963225E-5 7 3.3962006231933806E-4 9.433890619981612E-6 0.0 2.8301671859944837E-5 2.8301671859944837E-5 8 3.584878435593013E-4 9.433890619981612E-6 0.0 2.8301671859944837E-5 2.8301671859944837E-5 9 4.009403513492185E-4 9.433890619981612E-6 0.0 2.8301671859944837E-5 3.301861716993564E-5 10-11 4.5046827710412197E-4 1.650930858496782E-5 0.0 3.066014451494024E-5 4.245250778991726E-5 12-13 5.282978747189703E-4 2.358472654995403E-5 0.0 4.0094035134921854E-5 6.132028902988048E-5 14-15 6.981079058786394E-4 2.5943199204949433E-5 0.0 4.716945309990806E-5 8.018807026984371E-5 16-17 9.103704448282257E-4 2.8301671859944837E-5 0.0 5.188639840989887E-5 9.433890619981613E-5 18-19 0.0010377279681979773 2.8301671859944837E-5 0.0 5.188639840989887E-5 1.1320668743977935E-4 20-21 0.0011415007650177752 2.8301671859944837E-5 0.0 5.896181637488507E-5 1.4150835929972418E-4 22-23 0.0012547074524575544 2.8301671859944837E-5 0.0 7.075417964986209E-5 1.533007225747012E-4 24-25 0.0014032912297222647 3.301861716993564E-5 0.0 7.075417964986209E-5 1.6273461319468282E-4 26-27 0.0015778182061919246 3.773556247992645E-5 0.0 8.018807026984371E-5 1.8160239443464605E-4 28-29 0.001719326565491649 4.716945309990806E-5 0.0 8.490501557983452E-5 1.8867781239963224E-4 30-31 0.0019150797958562673 4.716945309990806E-5 0.0 9.198043354482073E-5 1.9811170301961385E-4 32-33 0.0021320592801158445 4.716945309990806E-5 4.716945309990806E-6 1.0141432416480233E-4 2.1462101160458168E-4 34-35 0.0024999810142951277 4.716945309990806E-5 4.716945309990806E-6 1.1084821478478395E-4 2.287718475345541E-4 36-37 0.00426883550554168 4.716945309990806E-5 4.716945309990806E-6 1.1320668743977935E-4 2.452811561195219E-4 38-39 0.004540059860866151 4.952792575490346E-5 4.716945309990806E-6 1.2028210540476555E-4 2.499981014295127E-4 40-41 0.0048702460325655075 5.188639840989887E-5 4.716945309990806E-6 1.2971599602474716E-4 2.6179046470448976E-4 42-43 0.005143828860544974 5.188639840989887E-5 4.716945309990806E-6 1.3679141398973338E-4 2.6414893735948514E-4 44-45 0.005400902379939473 5.188639840989887E-5 4.716945309990806E-6 1.3679141398973338E-4 2.80658245944453E-4 46-47 0.005900898582798499 5.188639840989887E-5 4.716945309990806E-6 1.3914988664472878E-4 2.971675545294208E-4 48-49 0.00649523369185734 5.188639840989887E-5 4.716945309990806E-6 1.485837772647104E-4 2.995260271844162E-4 50-51 0.006903249461171546 5.188639840989887E-5 4.716945309990806E-6 1.6509308584967822E-4 3.1839380842437945E-4 52-53 0.007374943992170625 5.188639840989887E-5 4.716945309990806E-6 1.8396086708964145E-4 3.2782769904436107E-4 54-55 0.00799286382777942 5.188639840989887E-5 4.716945309990806E-6 1.9339475770962304E-4 3.372615896643427E-4 56-57 0.008709839514898024 5.188639840989887E-5 4.716945309990806E-6 1.9339475770962304E-4 3.5141242559431506E-4 58-59 0.009497569381666487 5.896181637488507E-5 9.433890619981612E-6 2.0754559363959547E-4 3.632047888692921E-4 60-61 0.010549448185794438 6.603723433987128E-5 1.1792363274977016E-5 2.1226253894958628E-4 3.797140974542599E-4 62-63 0.011806514110906989 6.603723433987128E-5 1.4150835929972419E-5 2.1697948425957708E-4 3.9858187869422313E-4 64-65 0.013405558570993872 6.839570699486669E-5 1.4150835929972419E-5 2.1697948425957708E-4 4.127327146241955E-4 66-67 0.01538667560119001 7.54711249598529E-5 1.4150835929972419E-5 2.216964295695679E-4 4.26883550554168E-4 68-69 0.01794090148655003 7.54711249598529E-5 1.4150835929972419E-5 2.216964295695679E-4 4.5046827710412197E-4 70-71 0.021504553668248085 7.54711249598529E-5 1.4150835929972419E-5 2.287718475345541E-4 4.528267497591174E-4 72-73 0.02610357534548912 7.78295976148483E-5 1.4150835929972419E-5 2.311303201895495E-4 4.528267497591174E-4 74-75 0.03150919467073859 8.018807026984371E-5 1.4150835929972419E-5 2.3584726549954032E-4 4.669775856890898E-4 76-77 0.03853508470996989 8.018807026984371E-5 1.4150835929972419E-5 2.3584726549954032E-4 4.8112842161906225E-4 78-79 0.047138792955393125 8.018807026984371E-5 1.4150835929972419E-5 2.452811561195219E-4 4.8112842161906225E-4 80-81 0.05829908555883137 8.018807026984371E-5 1.4150835929972419E-5 2.499981014295127E-4 4.8584536692905305E-4 82-83 0.07246407232473376 8.018807026984371E-5 1.4150835929972419E-5 2.547150467395035E-4 5.023546755140208E-4 84-85 0.08983894037408491 8.254654292483911E-5 1.4150835929972419E-5 2.547150467395035E-4 5.165055114439933E-4 86-87 0.11075151740592915 8.490501557983452E-5 1.4150835929972419E-5 2.547150467395035E-4 5.282978747189703E-4 88-89 0.13741640924330717 8.490501557983452E-5 1.4150835929972419E-5 2.6414893735948514E-4 5.448071833039381E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 200760 0.0 94.28086 1 TCGACGG 203380 0.0 93.10369 2 CGACGGC 205940 0.0 91.84929 3 ACGGCGC 207805 0.0 90.965675 5 GACGGCG 208260 0.0 90.81276 4 CGGCGCG 208550 0.0 90.6079 6 TATCTCG 92615 0.0 88.94686 2 ATATCTC 93920 0.0 87.90317 1 ATCTCGA 98780 0.0 83.27582 3 TCTCGAG 100680 0.0 80.70231 4 CTCGAGG 103185 0.0 78.72451 5 TCGAGGG 104155 0.0 77.970345 6 CGAGGGC 105465 0.0 76.77071 7 AGGGCGC 106185 0.0 76.43786 9 GAGGGCG 108705 0.0 74.678986 8 CGCGCCG 312505 0.0 61.066216 9 GGCGCGC 314165 0.0 60.819283 7 GCGCGCC 314330 0.0 60.76606 8 TCCATAT 225180 0.0 41.905594 18-19 ATCCATA 228090 0.0 41.572952 16-17 >>END_MODULE