FastQCFastQC Report
Tue 31 May 2016
SRR1051285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21200161
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1366760.6446932171883034No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT284000.13396124680373891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG109000.082.036951
TCGACGG120000.074.73512
CGACGGC148100.060.618693
ACGGCGC168750.053.3109635
GACGGCG173850.051.694484
CGGCGCG180350.049.8816836
TATCTCG79950.048.3032072
ATCTCGA167400.042.48381
TCTCGAG202950.035.201622
ATATCTC112850.034.3323251
TCGAGGG241550.029.8905774
CTCGAGG240450.029.8496653
CGCGCCG437050.028.9956599
GGCGCGC450900.028.083957
GCGCGCC453750.027.9284928
CGAGGGC259650.027.8423985
GGGCGCG262250.027.4575618
AGGGCGC273950.026.3195677
GAGGGCG298750.024.2936486
GCCGGAT447300.017.06995612-13
Produced by FastQC (version 0.11.5)