##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051285_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21200161 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.60518247951042 34.0 31.0 34.0 31.0 34.0 2 32.83934886154874 34.0 31.0 34.0 31.0 34.0 3 32.891545304773864 34.0 31.0 34.0 31.0 34.0 4 36.32803076353996 37.0 37.0 37.0 35.0 37.0 5 36.208554218055234 37.0 37.0 37.0 35.0 37.0 6 36.176461207063475 37.0 37.0 37.0 35.0 37.0 7 36.14277235913444 37.0 36.0 37.0 35.0 37.0 8 36.12701101656728 37.0 36.0 37.0 35.0 37.0 9 37.83169104234633 39.0 38.0 39.0 35.0 39.0 10-11 37.80975653911308 39.0 38.0 39.0 35.0 39.0 12-13 37.78056970888098 39.0 38.0 39.0 35.0 39.0 14-15 39.27857955890052 41.0 39.0 41.0 36.0 41.0 16-17 39.28339508836749 41.0 39.0 41.0 36.0 41.0 18-19 39.32882790371262 41.0 39.0 41.0 36.0 41.0 20-21 39.33965685449276 41.0 39.0 41.0 36.0 41.0 22-23 39.32692013518199 41.0 39.0 41.0 36.0 41.0 24-25 39.275525666998476 41.0 39.0 41.0 36.0 41.0 26-27 39.18402485245277 40.0 39.0 41.0 36.0 41.0 28-29 39.063804185260665 40.0 38.5 41.0 35.5 41.0 30-31 38.91925125002589 40.0 38.0 41.0 35.0 41.0 32-33 38.809898118226556 40.0 38.0 41.0 35.0 41.0 34-35 38.76520963685134 40.0 38.0 41.0 35.0 41.0 36-37 38.77031648486066 40.0 38.0 41.0 35.0 41.0 38-39 38.70582650292137 40.0 38.0 41.0 35.0 41.0 40-41 38.665628718574354 40.0 38.0 41.0 35.0 41.0 42-43 38.65019385937683 40.0 38.0 41.0 35.0 41.0 44-45 38.5686329221745 40.0 38.0 41.0 34.5 41.0 46-47 38.45610493241065 40.0 38.0 41.0 34.0 41.0 48-49 38.36868378027884 40.0 38.0 41.0 34.0 41.0 50-51 38.279965185170056 40.0 38.0 41.0 34.0 41.0 52-53 38.22523819512503 40.0 37.0 41.0 34.0 41.0 54-55 38.181701002176354 40.0 37.0 41.0 34.0 41.0 56-57 37.97028883412725 40.0 37.0 41.0 34.0 41.0 58-59 37.75316246419072 40.0 36.5 41.0 33.0 41.0 60-61 37.493596628818054 39.0 36.0 41.0 33.0 41.0 62-63 37.16173134251197 39.0 35.0 41.0 33.0 41.0 64-65 36.82246771616499 38.5 35.0 40.5 32.0 41.0 66-67 36.459062881645096 37.5 35.0 40.0 32.0 41.0 68-69 36.064720216039866 37.0 35.0 39.5 31.5 41.0 70-71 35.69763281514702 36.5 35.0 39.0 31.0 41.0 72-73 35.30690172588784 36.0 35.0 39.0 31.0 40.5 74-75 34.904122284731706 35.5 34.0 37.5 31.0 39.5 76-77 34.311961545952414 35.0 34.0 37.0 30.0 39.0 78-79 34.13867345158369 35.0 34.0 37.0 30.0 39.0 80-81 33.869687451901896 35.0 34.0 36.0 30.0 37.5 82-83 33.59655485635227 35.0 34.0 36.0 30.0 37.0 84-85 33.32719982645415 35.0 34.0 35.5 30.0 37.0 86-87 33.091407819968914 35.0 34.0 35.0 29.0 36.0 88-89 32.927377202465586 35.0 34.0 35.0 29.0 36.0 90-91 32.730402047418416 35.0 33.0 35.0 29.0 36.0 92-93 32.56662588552983 35.0 33.0 35.0 29.0 36.0 94-95 32.40063297160809 35.0 33.0 35.0 29.0 35.0 96-97 32.21012003163561 35.0 33.0 35.0 29.0 35.0 98-99 32.008325974505574 35.0 33.0 35.0 27.5 35.0 100-101 31.068512946670545 34.5 31.5 35.0 23.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2918820438134304 1101 2 0.2756719401858234 1101 3 0.26543238232333266 1101 4 0.17254300807097422 1101 5 0.19645069768952794 1101 6 0.1909266575665285 1101 7 0.21645819129130217 1101 8 0.20315622583007098 1101 9 0.273157169233734 1101 10-11 0.22906538117000963 1101 12-13 0.23571441377164604 1101 14-15 0.31583594086046674 1101 16-17 0.31386116264923913 1101 18-19 0.27453557175972776 1101 20-21 0.2867040852718077 1101 22-23 0.2996280073943183 1101 24-25 0.2580990638092757 1101 26-27 0.23064553932137244 1101 28-29 0.2481676989713435 1101 30-31 0.23429647275756338 1101 32-33 0.25759109295771765 1101 34-35 0.2360454524729647 1101 36-37 0.21104539338020345 1101 38-39 0.1783187162861637 1101 40-41 0.2169359046831687 1101 42-43 0.15465179636836268 1101 44-45 0.1338178025841117 1101 46-47 0.10887728201689129 1101 48-49 0.06132088018469517 1101 50-51 -0.019440330979854537 1101 52-53 0.014707224052898482 1101 54-55 0.10895818870194063 1101 56-57 0.13666368824010533 1101 58-59 0.12213070398946257 1101 60-61 0.08194826398300137 1101 62-63 0.009384545140989076 1101 64-65 0.00588007700867621 1101 66-67 0.026164009175019487 1101 68-69 -0.02370691226729349 1101 70-71 -0.0962809976418697 1101 72-73 -0.1258362929438661 1101 74-75 -0.17511741592007013 1101 76-77 -0.29926118600563 1101 78-79 -0.2936662724483483 1101 80-81 -0.37293936682900153 1101 82-83 -0.3538460699381716 1101 84-85 -0.4267639091277502 1101 86-87 -0.6233529918095542 1101 88-89 -0.7018435973540065 1101 90-91 -0.7970545398965392 1101 92-93 -0.9032607820395171 1101 94-95 -1.1022037175772397 1101 96-97 -1.3336154889917253 1101 98-99 -1.5337960846724812 1101 100-101 -1.7611041065122421 1102 1 0.29166024535780366 1102 2 0.24452652419491017 1102 3 0.23676407742738093 1102 4 0.16030160028187623 1102 5 0.183782958620462 1102 6 0.2128260577975496 1102 7 0.16248175804228993 1102 8 0.17629083392022693 1102 9 0.23631539304709293 1102 10-11 0.22135839113406774 1102 12-13 0.2262420185089269 1102 14-15 0.2712749552620153 1102 16-17 0.23087487950801489 1102 18-19 0.2808859060431601 1102 20-21 0.252443990347885 1102 22-23 0.2280635967631781 1102 24-25 0.2201248803294007 1102 26-27 0.23684957259010275 1102 28-29 0.2292104634301282 1102 30-31 0.2339846983004179 1102 32-33 0.2261377634591497 1102 34-35 0.1964384202465297 1102 36-37 0.17448462941028708 1102 38-39 0.18855123946220687 1102 40-41 0.1858679040723814 1102 42-43 0.15418595024817705 1102 44-45 0.08185611501433954 1102 46-47 0.030730636385989385 1102 48-49 0.038156000829829395 1102 50-51 -0.006276859645851118 1102 52-53 0.02018158243851076 1102 54-55 0.14353698188529762 1102 56-57 0.13293855307867375 1102 58-59 0.09250418023587059 1102 60-61 0.07415084527286808 1102 62-63 0.10075267941834909 1102 64-65 0.021991402779484304 1102 66-67 -0.014090367225847444 1102 68-69 -0.0935403194001978 1102 70-71 -0.09145589997704917 1102 72-73 -0.0846699524731136 1102 74-75 -0.17361568902961722 1102 76-77 -0.2683815403951044 1102 78-79 -0.30161559417130945 1102 80-81 -0.3468800358744488 1102 82-83 -0.413319842022176 1102 84-85 -0.435142392379376 1102 86-87 -0.5495406199429027 1102 88-89 -0.7248153985918222 1102 90-91 -0.8788658645154612 1102 92-93 -0.9382962954624361 1102 94-95 -1.1362671923947332 1102 96-97 -1.3407863836836142 1102 98-99 -1.5423920834000384 1102 100-101 -1.8180888629070147 1103 1 0.2593781611674615 1103 2 0.23900422512669905 1103 3 0.2321303392213352 1103 4 0.14290226690106778 1103 5 0.19196207779365437 1103 6 0.1429523029958375 1103 7 0.1466596409189478 1103 8 0.15130627235430438 1103 9 0.199930891908366 1103 10-11 0.21347295784206466 1103 12-13 0.1987109422240323 1103 14-15 0.2777891082653525 1103 16-17 0.30022820679164397 1103 18-19 0.276892609762065 1103 20-21 0.25114503026474466 1103 22-23 0.25466363685598026 1103 24-25 0.2563614858078509 1103 26-27 0.2627613674664673 1103 28-29 0.20540766206560335 1103 30-31 0.2295293137167178 1103 32-33 0.23832168355770023 1103 34-35 0.24601481893898125 1103 36-37 0.15004483555047443 1103 38-39 0.12709705591711185 1103 40-41 0.10802088097598528 1103 42-43 0.08630421816583578 1103 44-45 0.06818472273813114 1103 46-47 0.01580193463524182 1103 48-49 0.02265737737262441 1103 50-51 -0.03566165637517571 1103 52-53 0.02314717995216853 1103 54-55 0.1522199206963819 1103 56-57 0.22174515383395743 1103 58-59 0.1604277309255977 1103 60-61 0.11850152166653771 1103 62-63 0.10978630806300771 1103 64-65 0.08827144532637732 1103 66-67 0.02727366162420708 1103 68-69 0.019965229708859056 1103 70-71 -0.025922241136242974 1103 72-73 -0.11372057343154296 1103 74-75 -0.13779826452696398 1103 76-77 -0.22986878697438584 1103 78-79 -0.2613154558275852 1103 80-81 -0.28789224035367056 1103 82-83 -0.3329853088720398 1103 84-85 -0.41934933904634875 1103 86-87 -0.5468344758301384 1103 88-89 -0.6097301459705236 1103 90-91 -0.7729869607472608 1103 92-93 -0.8894424071937621 1103 94-95 -1.0433859097636997 1103 96-97 -1.23556174355884 1103 98-99 -1.453351261789095 1103 100-101 -1.7453485556952657 1104 1 0.2258136511705544 1104 2 0.19812338970741195 1104 3 0.19932569053865024 1104 4 0.1283584678056755 1104 5 0.16307342550240378 1104 6 0.1836093812588473 1104 7 0.17356479004374847 1104 8 0.14008502344236717 1104 9 0.2173202162650938 1104 10-11 0.22741248232657085 1104 12-13 0.20159110594252638 1104 14-15 0.23628580585526038 1104 16-17 0.23175884103552846 1104 18-19 0.21650631898263129 1104 20-21 0.24529266927673632 1104 22-23 0.2405524409520723 1104 24-25 0.23470746810819065 1104 26-27 0.23794839756433817 1104 28-29 0.20304283501239695 1104 30-31 0.20446360400072905 1104 32-33 0.19558467919935651 1104 34-35 0.22612851708664294 1104 36-37 0.17667854856499332 1104 38-39 0.16437163948317846 1104 40-41 0.13614461372547026 1104 42-43 0.11494617764113713 1104 44-45 0.11498223728938228 1104 46-47 0.059656650637194275 1104 48-49 0.08619955375745292 1104 50-51 0.004039582642754169 1104 52-53 0.005760862518201293 1104 54-55 0.11794310070251157 1104 56-57 0.16141336643022441 1104 58-59 0.11851923407136411 1104 60-61 0.10917762443473578 1104 62-63 0.14876765927397173 1104 64-65 0.10343202163179654 1104 66-67 0.060409123195803716 1104 68-69 0.02993294324878093 1104 70-71 -0.03209311878771359 1104 72-73 -0.0973312895870535 1104 74-75 -0.12991056079220442 1104 76-77 -0.2439063770892389 1104 78-79 -0.23915208961501833 1104 80-81 -0.29662247754450277 1104 82-83 -0.3057995412507495 1104 84-85 -0.30580613327947503 1104 86-87 -0.41124963283593274 1104 88-89 -0.5495820535331362 1104 90-91 -0.667285068560723 1104 92-93 -0.7396610282143463 1104 94-95 -0.9061040136068286 1104 96-97 -1.0607908896378646 1104 98-99 -1.2654569214409754 1104 100-101 -1.4948013322220959 1105 1 0.12981413271830178 1105 2 0.13791125095615087 1105 3 0.1308925093428286 1105 4 0.08938360499550413 1105 5 0.09078563929413974 1105 6 0.08483369278329178 1105 7 0.10043954676628886 1105 8 0.0821703904531077 1105 9 0.12535819216869726 1105 10-11 0.14571215322777675 1105 12-13 0.17480484596892154 1105 14-15 0.19829508209296876 1105 16-17 0.16591405147828908 1105 18-19 0.11375081663644693 1105 20-21 0.12493385015768865 1105 22-23 0.07337730056464409 1105 24-25 0.07541559674479714 1105 26-27 0.11248005774476866 1105 28-29 0.1054837016415533 1105 30-31 0.1330567661193527 1105 32-33 0.10325895446005973 1105 34-35 0.08796597614259127 1105 36-37 0.054630875767443854 1105 38-39 0.0643470063398155 1105 40-41 0.05111388901382696 1105 42-43 0.03911155439023162 1105 44-45 -0.016440007112954902 1105 46-47 -0.013587833163008156 1105 48-49 0.022086166767621762 1105 50-51 -0.08112221723967394 1105 52-53 0.02543149738961148 1105 54-55 0.07054978664505285 1105 56-57 0.09519841120455652 1105 58-59 0.07510538217425022 1105 60-61 0.041281698136458544 1105 62-63 0.014308718817559907 1105 64-65 0.037940311604081955 1105 66-67 0.01506280858521336 1105 68-69 -0.03801928891135731 1105 70-71 -0.07313267932062217 1105 72-73 -0.05990016103684326 1105 74-75 -0.09709142251244884 1105 76-77 -0.2210011648342416 1105 78-79 -0.18818518705546694 1105 80-81 -0.2512524775289364 1105 82-83 -0.21585950221466987 1105 84-85 -0.29471933134449557 1105 86-87 -0.3605439704669493 1105 88-89 -0.435420415115253 1105 90-91 -0.553341259620062 1105 92-93 -0.6411751436364206 1105 94-95 -0.739624644743671 1105 96-97 -0.8894392487912981 1105 98-99 -1.0319661829927682 1105 100-101 -1.1917517463891372 1106 1 0.02085100020448749 1106 2 0.03193256019036994 1106 3 0.036812736696511195 1106 4 0.028500045646978833 1106 5 0.05923907413196616 1106 6 0.06066289902754818 1106 7 0.06688374182390788 1106 8 0.013807813228986276 1106 9 0.07105911745235005 1106 10-11 0.053524198492048924 1106 12-13 0.07468314748662408 1106 14-15 0.10256568639897523 1106 16-17 0.08419516935410343 1106 18-19 0.07302712861089589 1106 20-21 0.07274252430771355 1106 22-23 0.027567112296537744 1106 24-25 0.04945318565804513 1106 26-27 0.03874782314743186 1106 28-29 0.008589004782280085 1106 30-31 0.04887561160416709 1106 32-33 0.04186025073482824 1106 34-35 0.04147549374008719 1106 36-37 0.021052394495264082 1106 38-39 -0.017770997563239632 1106 40-41 -0.024152572172134512 1106 42-43 -0.04190275552757328 1106 44-45 -0.052358430854788196 1106 46-47 -0.06480606160643276 1106 48-49 -0.07706627347171491 1106 50-51 -0.08185500067310869 1106 52-53 -0.042474773422455314 1106 54-55 0.040924322335492036 1106 56-57 0.049361487658458714 1106 58-59 -0.01511863208481401 1106 60-61 -0.04420846946877077 1106 62-63 -0.06564344135259148 1106 64-65 -0.04594050371242275 1106 66-67 -0.013949187064284274 1106 68-69 -0.02846830395141353 1106 70-71 -0.04958913599184456 1106 72-73 -0.10886587287495786 1106 74-75 -0.06016001752496436 1106 76-77 -0.17033165763644575 1106 78-79 -0.09263782425185241 1106 80-81 -0.11238141367027765 1106 82-83 -0.11887872328664173 1106 84-85 -0.1441500802024933 1106 86-87 -0.21007806049229316 1106 88-89 -0.2703913357566776 1106 90-91 -0.3425370751995871 1106 92-93 -0.36591263185970746 1106 94-95 -0.4238550447914733 1106 96-97 -0.5163026135174462 1106 98-99 -0.6383995526337856 1106 100-101 -0.7350815763252143 1107 1 -0.02959286421916829 1107 2 -0.017572669403662644 1107 3 -0.009348202940770989 1107 4 0.016364026015864397 1107 5 -0.015776745499636036 1107 6 -0.008189887131244689 1107 7 0.008930398200938328 1107 8 0.010866493654233977 1107 9 -0.015460415896960455 1107 10-11 0.02098949664149785 1107 12-13 0.030419356657759522 1107 14-15 0.012739250459269158 1107 16-17 0.014188771174623582 1107 18-19 -0.021716455431281645 1107 20-21 -0.012481681363723851 1107 22-23 -0.027616322322444375 1107 24-25 -0.022955311250562715 1107 26-27 -0.05143383580835348 1107 28-29 -0.06288300047093998 1107 30-31 -0.1135153968968936 1107 32-33 -0.07747660935797285 1107 34-35 -0.09561836620468256 1107 36-37 -0.08214544773919386 1107 38-39 -0.1351683998607669 1107 40-41 -0.16576287351135477 1107 42-43 -0.1039236187068795 1107 44-45 -0.11099724097685737 1107 46-47 -0.09382483947524634 1107 48-49 -0.1345446212944239 1107 50-51 -0.06459639012606289 1107 52-53 -0.065262690691128 1107 54-55 -0.0234435483428328 1107 56-57 -0.025080688915856797 1107 58-59 -0.03968038189041323 1107 60-61 -0.05212442976410614 1107 62-63 -0.018160168519493425 1107 64-65 -0.03692783442642167 1107 66-67 0.05285225926277093 1107 68-69 -0.021974211967020096 1107 70-71 -0.023904616475164175 1107 72-73 -0.057898504216616686 1107 74-75 -0.08269620583931214 1107 76-77 -0.19977460954061854 1107 78-79 -0.13273268041444197 1107 80-81 -0.13451996305470715 1107 82-83 -0.15219391264520254 1107 84-85 -0.10903573614415052 1107 86-87 -0.08633827871263833 1107 88-89 -0.1379525137737332 1107 90-91 -0.22572286821329612 1107 92-93 -0.22487702166080936 1107 94-95 -0.2604747402518939 1107 96-97 -0.3306706089990925 1107 98-99 -0.4271221175275919 1107 100-101 -0.38360205563467886 1108 1 -0.12305181371099394 1108 2 -0.1038005540728335 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-0.5251091459080897 2308 22-23 -0.6196401884971863 2308 24-25 -0.5433042911634871 2308 26-27 -0.5454662303702662 2308 28-29 -0.586894601935704 2308 30-31 -0.586213871210866 2308 32-33 -0.5670011984396623 2308 34-35 -0.7165292977960576 2308 36-37 -1.0280416533353076 2308 38-39 -0.9177915105661896 2308 40-41 -0.7381294787468846 2308 42-43 -0.8039316859735237 2308 44-45 -0.6760365840004994 2308 46-47 -0.6250139530507823 2308 48-49 -0.5528055576399993 2308 50-51 -0.4445638138902481 2308 52-53 -0.5503159990180109 2308 54-55 -0.6195825938400574 2308 56-57 -0.7845369441446621 2308 58-59 -0.7263049597160176 2308 60-61 -0.8394222597437277 2308 62-63 -0.9703441622476987 2308 64-65 -0.8287498035295968 2308 66-67 -0.9320055876771534 2308 68-69 -0.9526690279930108 2308 70-71 -1.362577060924778 2308 72-73 -1.1153206507650282 2308 74-75 -1.0141431613154168 2308 76-77 -1.3260924073750218 2308 78-79 -1.1027977487608212 2308 80-81 -1.0368543342588907 2308 82-83 -1.1075407235317698 2308 84-85 -1.1047810881315954 2308 86-87 -1.1253228839113447 2308 88-89 -1.1616874795907606 2308 90-91 -1.0270374695931608 2308 92-93 -0.905835788344497 2308 94-95 -0.9744548708216314 2308 96-97 -0.9938342773910662 2308 98-99 -1.1854598555552442 2308 100-101 -1.0626427739128914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 5.0 11 79.0 12 347.0 13 1178.0 14 3200.0 15 6712.0 16 11177.0 17 18637.0 18 35737.0 19 65774.0 20 64807.0 21 56851.0 22 55259.0 23 57735.0 24 64946.0 25 78966.0 26 100216.0 27 127718.0 28 163329.0 29 209515.0 30 269668.0 31 349758.0 32 465456.0 33 639631.0 34 934168.0 35 1471564.0 36 2463760.0 37 5151890.0 38 6879095.0 39 1452065.0 40 917.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.155515937557656 29.857902062722957 24.68700743054623 22.299574569173156 2 24.07220622235975 28.39392382063351 26.872180844803744 20.661689112202996 3 23.13508078543411 27.05157681961743 26.60212026888431 23.211222126064154 4 24.362568242266757 26.638066762585638 26.51901396605012 22.480351029097488 5 24.420472537142423 27.644972373579673 25.27330545101455 22.661249638263353 6 24.424125835648137 26.71525937939811 24.698194508994533 24.162420275959224 7 24.618256436826115 26.87278176802525 25.311189853699695 23.197771941448934 8 24.181835222855145 26.53956260049157 25.48012724997702 23.798474926676263 9 23.572297399062204 26.249602538395816 25.820417118530376 24.357682944011604 10-11 23.8825721126633 26.582591829495627 25.699558399559248 23.835277658281825 12-13 23.708989342030193 26.52060697039117 25.948858508959695 23.821545178618948 14-15 23.552354154291564 26.58156699847704 26.03150513809777 23.834573709133625 16-17 23.625857455692962 26.79056324093193 25.99779246063988 23.585786842735228 18-19 23.26487756390152 26.64382831809626 26.423186125803475 23.66810799219874 20-21 23.041780201574884 26.72142678539092 26.46590514100341 23.770887872030784 22-23 22.976959939124992 26.60330268246548 27.006101510266834 23.4136358681427 24-25 22.988629661821907 26.324934513469024 27.47702482070773 23.20941100400134 26-27 22.98193631642703 26.335198586463566 27.623660499559417 23.05920459754999 28-29 22.971264227663177 26.42797618376577 27.61641055461796 22.98434903395309 30-31 22.982716180758466 26.392369505559788 27.592457910609248 23.032456403072498 32-33 22.980431610873143 26.438773271580345 27.59672910031202 22.984066017234493 34-35 22.984422146605397 26.50184354732023 27.567387342011223 22.94634696406315 36-37 22.99889373481645 26.505341162267587 27.48788794575664 23.007877157159324 38-39 22.99296453456179 26.531991903269038 27.42732944339432 23.047714118774852 40-41 23.090789129997695 26.69224360512663 27.15253651665353 23.064430748222147 42-43 23.090697107013735 26.717819600590136 27.08621553547787 23.10526775691826 44-45 23.08092706292652 26.831087204224445 27.010938381779496 23.077047351069535 46-47 23.10457259450825 26.985296002694703 26.840947759752016 23.06918364304503 48-49 23.13010264403181 26.98929267565468 26.73109416480375 23.149510515509764 50-51 23.119435272213263 26.993028496340195 26.73534884947336 23.15218738197318 52-53 23.120640451739966 27.0623864601783 26.715525886808123 23.10144720127361 54-55 23.127865191180668 26.996223518355244 26.69086727475024 23.185044015713846 56-57 23.135535121955222 27.032598058938994 26.6788766385609 23.15299018054488 58-59 23.140587887211204 27.103787684571174 26.656015014984558 23.099609413233065 60-61 23.128326714122597 27.060200156027115 26.643941524783703 23.167531605066586 62-63 23.13799881048073 27.09858665695982 26.64885894026937 23.114555592290078 64-65 23.14292580321304 27.17393998718985 26.614700411312192 23.068433798284925 66-67 23.10576073979249 27.144551676083523 26.61315577322322 23.136531810900767 68-69 23.108318926416917 27.17957411754129 26.5978846962722 23.114222259769594 70-71 23.116252463851367 27.251975597594363 26.585948583778684 23.04582335477559 72-73 23.07958418831833 27.233389670774205 26.58490886303959 23.102117277867876 74-75 23.076842344165282 27.24756609648482 26.607794005386637 23.06779755396326 76-77 23.104233697534085 27.322346414909614 26.556338994480267 23.017080893076034 78-79 23.07461899387983 27.262514709143687 26.569485354441746 23.093380942534733 80-81 23.040741301294858 27.303109120098355 26.585584125182855 23.07056545342393 82-83 23.052763038351486 27.384162590716958 26.580359624155303 22.98271474677625 84-85 23.021452169032834 27.33641652720974 26.595481133244032 23.046650170513395 86-87 23.007283839064034 27.423846528155266 26.569050386781484 22.999819245999216 88-89 23.03210807132821 27.45584381049192 26.55091212158555 22.961135996594322 90-91 23.022721825644226 27.43584773788591 26.511352422190793 23.030078014279074 92-93 22.990388401852694 27.461532096933755 26.543322478089976 23.004757023123574 94-95 23.01057643385323 27.52909000459734 26.508820660547382 22.951512901002047 96-97 22.962621252321316 27.555339375835857 26.485299292936492 22.99674007890633 98-99 22.969447705474114 27.611943255442206 26.46207652054864 22.956532518535038 100-101 23.003470267045635 27.655374338439 26.342458722845784 22.998696671669585 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 117255.0 1 63978.5 2 7497.0 3 3578.5 4 2560.0 5 2119.5 6 1897.5 7 1776.0 8 1727.0 9 1698.0 10 1755.5 11 1883.5 12 2302.5 13 3415.5 14 4382.5 15 4969.0 16 13185.0 17 13561.0 18 6148.5 19 6757.0 20 8818.5 21 12134.5 22 16701.5 23 23159.0 24 30916.0 25 40584.0 26 53219.0 27 69460.0 28 89203.5 29 114080.0 30 145037.5 31 179827.0 32 219921.5 33 271305.0 34 327278.5 35 386260.0 36 455374.0 37 522492.0 38 578780.0 39 632767.5 40 685387.0 41 725411.0 42 757809.0 43 794235.5 44 830719.0 45 865114.5 46 884052.5 47 890795.0 48 895001.0 49 891098.5 50 873390.5 51 846698.5 52 824200.0 53 801633.5 54 761229.0 55 703532.5 56 639679.0 57 568811.0 58 499331.0 59 429139.5 60 355782.5 61 292626.5 62 238706.0 63 186778.5 64 143890.5 65 109836.0 66 80731.5 67 58812.0 68 44026.0 69 33441.0 70 25636.0 71 20278.5 72 16798.0 73 13029.5 74 9410.0 75 7108.0 76 5369.5 77 3756.0 78 2449.5 79 1446.5 80 712.0 81 405.5 82 263.0 83 188.0 84 124.0 85 83.5 86 39.0 87 9.0 88 7.5 89 3.5 90 1.5 91 1.0 92 1.5 93 1.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08428709574422572 2 0.0015377241710570028 3 5.377317653389519E-4 4 0.0015235733351270304 5 0.001632063077256819 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.716945309990806E-6 12-13 7.78295976148483E-5 14-15 0.0 16-17 4.056572966592094E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 6.839570699486669E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.4669548028432425E-4 42-43 4.0094035134921854E-5 44-45 6.273537262287772E-4 46-47 1.9575323036461847E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.3584726549954033E-5 56-57 1.4150835929972417E-5 58-59 2.8301671859944834E-5 60-61 0.0 62-63 0.0 64-65 0.002691017299349755 66-67 2.547150467395035E-4 68-69 0.0012924430149374808 70-71 5.660334371988967E-4 72-73 0.0010731050580229085 74-75 5.259394020639749E-4 76-77 1.8867781239963226E-4 78-79 0.001591969042121897 80-81 0.00424289230633673 82-83 0.0023608311276503984 84-85 3.16035335769384E-4 86-87 3.6320478886929205E-4 88-89 0.0012900845422824853 90-91 0.0015282902804370213 92-93 0.005608447973579069 94-95 0.00450468277104122 96-97 0.0034032760411583664 98-99 0.0014764038820271222 100-101 9.787661518230923E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.1200161E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.2102527394053 #Duplication Level Percentage of deduplicated Percentage of total 1 74.75992033720722 42.02274016932223 2 13.768457068249749 15.478569032759392 3 5.10828447932226 8.614138849424569 4 2.2971474978740343 5.164929657407679 5 1.2343676520674793 3.469205884802965 6 0.7517462761810001 2.5353508908024476 7 0.4843885038104277 1.9059320156272568 8 0.31838710251531993 1.431729560108246 9 0.22986766504589515 1.1628827593962054 >10 0.909636270136958 8.809008687960182 >50 0.07849725693761705 3.1014717265618037 >100 0.05895508208429733 5.113916891987367 >500 1.9352536646987544E-4 0.07219001515783068 >1k 1.0091178363758433E-4 0.12439907085493379 >5k 1.679047247884331E-5 0.06976545218595377 >10k+ 3.358094495768574E-5 0.9237693356407783 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 136676 0.6446932171883034 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28400 0.13396124680373891 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.3584726549954032E-4 0.0 0.0 4.716945309990806E-6 0.0 2 2.452811561195219E-4 0.0 0.0 4.716945309990806E-6 4.716945309990806E-6 3 2.5943199204949433E-4 0.0 0.0 1.4150835929972419E-5 1.4150835929972419E-5 4 2.6414893735948514E-4 4.716945309990806E-6 0.0 1.4150835929972419E-5 1.8867781239963225E-5 5 2.6414893735948514E-4 4.716945309990806E-6 0.0 1.4150835929972419E-5 2.8301671859944837E-5 6 2.7358282797946675E-4 4.716945309990806E-6 0.0 1.8867781239963225E-5 3.773556247992645E-5 7 2.7829977328945756E-4 4.716945309990806E-6 0.0 1.8867781239963225E-5 4.245250778991726E-5 8 2.877336639094392E-4 9.433890619981612E-6 0.0 2.358472654995403E-5 5.188639840989887E-5 9 3.3018617169935645E-4 1.8867781239963225E-5 0.0 2.358472654995403E-5 6.132028902988048E-5 10-11 3.679217341792829E-4 1.8867781239963225E-5 0.0 2.358472654995403E-5 7.31126523048575E-5 12-13 4.221666052441771E-4 2.358472654995403E-5 0.0 2.5943199204949433E-5 8.726348823482992E-5 14-15 6.014105270238278E-4 2.5943199204949433E-5 0.0 3.301861716993564E-5 9.669737885481152E-5 16-17 8.53767101108336E-4 2.8301671859944837E-5 0.0 4.245250778991726E-5 1.1084821478478395E-4 18-19 9.551814252731383E-4 2.8301671859944837E-5 0.0 4.716945309990806E-5 1.2028210540476555E-4 20-21 0.001030652550232991 3.301861716993564E-5 0.0 4.716945309990806E-5 1.2971599602474716E-4 22-23 0.0011202745111228165 3.773556247992645E-5 0.0 4.716945309990806E-5 1.3443294133473797E-4 24-25 0.0012499905071475638 3.773556247992645E-5 0.0 5.188639840989887E-5 1.4150835929972418E-4 26-27 0.0014221590109622282 3.773556247992645E-5 4.716945309990806E-6 5.188639840989887E-5 1.485837772647104E-4 28-29 0.0015707427882269386 3.773556247992645E-5 4.716945309990806E-6 5.424487106489427E-5 1.556591952296966E-4 30-31 0.0017594206006265709 3.773556247992645E-5 4.716945309990806E-6 6.132028902988048E-5 1.6273461319468282E-4 32-33 0.001971683139576157 4.0094035134921854E-5 4.716945309990806E-6 7.075417964986209E-5 1.7216850381466444E-4 34-35 0.002316020147205486 4.245250778991726E-5 4.716945309990806E-6 7.54711249598529E-5 1.7688544912465524E-4 36-37 0.003976384896322249 4.245250778991726E-5 4.716945309990806E-6 8.018807026984371E-5 1.8396086708964145E-4 38-39 0.004254684669611707 4.481098044491266E-5 4.716945309990806E-6 9.198043354482073E-5 1.9575323036461845E-4 40-41 0.00459194625927605 5.188639840989887E-5 4.716945309990806E-6 1.0377279681979773E-4 1.9811170301961385E-4 42-43 0.004867887559910512 5.188639840989887E-5 4.716945309990806E-6 1.1320668743977934E-4 2.0282864832960466E-4 44-45 0.005106093298065047 5.188639840989887E-5 4.716945309990806E-6 1.2264057805976095E-4 2.311303201895495E-4 46-47 0.005594297137649096 5.6603343719889675E-5 4.716945309990806E-6 1.2971599602474716E-4 2.4292268346452653E-4 48-49 0.006165047520157984 5.6603343719889675E-5 4.716945309990806E-6 1.4150835929972418E-4 2.547150467395035E-4 50-51 0.006556553980887221 5.6603343719889675E-5 4.716945309990806E-6 1.438668319547196E-4 2.712243553244714E-4 52-53 0.0070282485118863015 5.6603343719889675E-5 4.716945309990806E-6 1.46225304609715E-4 2.80658245944453E-4 54-55 0.007646168347495097 5.6603343719889675E-5 4.716945309990806E-6 1.485837772647104E-4 3.018844998394116E-4 56-57 0.008330125417443764 6.132028902988048E-5 4.716945309990806E-6 1.58017667884692E-4 3.066014451494024E-4 58-59 0.00909191208500728 6.603723433987128E-5 4.716945309990806E-6 1.7216850381466444E-4 3.113183904593932E-4 60-61 0.010124923107895267 6.603723433987128E-5 4.716945309990806E-6 1.8396086708964145E-4 3.113183904593932E-4 62-63 0.011358404306457862 6.603723433987128E-5 4.716945309990806E-6 1.8631933974463683E-4 3.2546922638936564E-4 64-65 0.012917354731409822 7.075417964986209E-5 4.716945309990806E-6 1.9103628505462764E-4 3.372615896643427E-4 66-67 0.014907905652225942 7.54711249598529E-5 4.716945309990806E-6 2.0047017567460926E-4 3.537708982493105E-4 68-69 0.017391377357936104 7.54711249598529E-5 4.716945309990806E-6 2.1226253894958628E-4 3.632047888692921E-4 70-71 0.020926727867774213 7.54711249598529E-5 4.716945309990806E-6 2.1462101160458168E-4 3.632047888692921E-4 72-73 0.025513957181740272 7.54711249598529E-5 4.716945309990806E-6 2.216964295695679E-4 3.702802068342783E-4 74-75 0.030905425671059764 7.54711249598529E-5 4.716945309990806E-6 2.311303201895495E-4 3.891479880742415E-4 76-77 0.03779216582364634 8.726348823482992E-5 4.716945309990806E-6 2.311303201895495E-4 3.962234060392277E-4 78-79 0.046289742799594774 8.962196088982532E-5 4.716945309990806E-6 2.3584726549954032E-4 3.9858187869422313E-4 80-81 0.05724248980939343 8.962196088982532E-5 4.716945309990806E-6 2.4056421080953112E-4 4.1037424196920013E-4 82-83 0.0711574784738663 8.962196088982532E-5 4.716945309990806E-6 2.4056421080953112E-4 4.2924202320916336E-4 84-85 0.08823046202337803 9.669737885481154E-5 4.716945309990806E-6 2.452811561195219E-4 4.4103438648414035E-4 86-87 0.10879398510228294 1.0377279681979773E-4 4.716945309990806E-6 2.547150467395035E-4 4.433928591391358E-4 88-89 0.13496123920945696 1.0377279681979773E-4 4.716945309990806E-6 2.712243553244714E-4 4.528267497591174E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 10900 0.0 82.03695 1 TCGACGG 12000 0.0 74.7351 2 CGACGGC 14810 0.0 60.61869 3 ACGGCGC 16875 0.0 53.310963 5 GACGGCG 17385 0.0 51.69448 4 CGGCGCG 18035 0.0 49.881683 6 TATCTCG 7995 0.0 48.303207 2 ATCTCGA 16740 0.0 42.4838 1 TCTCGAG 20295 0.0 35.20162 2 ATATCTC 11285 0.0 34.332325 1 TCGAGGG 24155 0.0 29.890577 4 CTCGAGG 24045 0.0 29.849665 3 CGCGCCG 43705 0.0 28.995659 9 GGCGCGC 45090 0.0 28.08395 7 GCGCGCC 45375 0.0 27.928492 8 CGAGGGC 25965 0.0 27.842398 5 GGGCGCG 26225 0.0 27.457561 8 AGGGCGC 27395 0.0 26.319567 7 GAGGGCG 29875 0.0 24.293648 6 GCCGGAT 44730 0.0 17.069956 12-13 >>END_MODULE