FastQCFastQC Report
Tue 31 May 2016
SRR1051284_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051284_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11238556
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT2253692.005319900528146No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT338390.3010974007692803No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA154880.13781129888928792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG811000.093.913411
TCGACGG826800.092.141722
CGACGGC838550.090.756733
ACGGCGC848300.089.751615
GACGGCG851500.089.4370354
CGGCGCG853450.089.1814046
TATCTCG435650.088.44442
ATATCTC442950.087.115491
ATCTCGA469050.082.05243
TCTCGAG475700.079.436734
CTCGAGG488400.077.41945
TCGAGGG491800.076.878696
CGAGGGC501600.075.186297
AGGGCGC503000.075.099459
GAGGGCG516750.073.128728
CGCGCCG1342700.057.3780259
GCGCGCC1349350.057.0952538
GGCGCGC1350600.057.0778277
CTCGACG16200.041.0511555
TCCATAT949800.040.08160418-19
Produced by FastQC (version 0.11.5)