##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051284_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11238556 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21765652099789 33.0 31.0 34.0 31.0 34.0 2 32.37855815284455 34.0 31.0 34.0 31.0 34.0 3 32.353721688088754 34.0 31.0 34.0 31.0 34.0 4 36.04840550689964 37.0 35.0 37.0 35.0 37.0 5 36.00146264342145 37.0 35.0 37.0 35.0 37.0 6 36.05541085527358 37.0 37.0 37.0 35.0 37.0 7 36.04376140493494 37.0 37.0 37.0 35.0 37.0 8 36.05087886735627 37.0 37.0 37.0 35.0 37.0 9 37.75675033340582 39.0 38.0 39.0 35.0 39.0 10-11 37.735185730266416 39.0 38.0 39.0 35.0 39.0 12-13 37.72459362216996 39.0 38.0 39.0 35.0 39.0 14-15 39.16797491599455 41.0 39.0 41.0 36.0 41.0 16-17 39.0979654770595 40.5 39.0 41.0 36.0 41.0 18-19 39.11500578899994 40.5 39.0 41.0 36.0 41.0 20-21 39.134014592266126 41.0 39.0 41.0 36.0 41.0 22-23 39.040763777837654 40.5 39.0 41.0 35.5 41.0 24-25 39.00342997801497 40.5 39.0 41.0 35.0 41.0 26-27 38.97233492452233 40.0 39.0 41.0 35.0 41.0 28-29 38.76135639667587 40.0 38.0 41.0 35.0 41.0 30-31 38.37043980561204 40.0 37.0 41.0 34.5 41.0 32-33 38.11663228799145 40.0 36.5 41.0 34.0 41.0 34-35 37.84785331852242 40.0 35.5 41.0 34.0 41.0 36-37 37.71161917954584 40.0 35.0 41.0 33.0 41.0 38-39 37.48170832622981 40.0 35.0 41.0 33.0 41.0 40-41 36.74477811028392 40.0 35.0 41.0 30.0 41.0 42-43 36.83635419888462 40.0 35.0 41.0 30.0 41.0 44-45 36.70815525588875 40.0 35.0 41.0 30.0 41.0 46-47 36.72241513945386 39.0 35.0 41.0 30.0 41.0 48-49 36.672388071919556 40.0 35.0 41.0 29.0 41.0 50-51 36.53671864072217 39.0 35.0 40.5 29.0 41.0 52-53 36.625589310584026 39.0 35.0 40.5 30.0 41.0 54-55 36.525361621190484 39.0 35.0 41.0 29.5 41.0 56-57 36.22020649272024 39.0 35.0 41.0 29.0 41.0 58-59 35.93315226618081 39.0 35.0 41.0 28.5 41.0 60-61 35.62982521953888 39.0 35.0 41.0 28.0 41.0 62-63 35.350957453964725 38.0 35.0 40.5 28.0 41.0 64-65 35.02783702817337 37.5 34.5 40.0 27.0 41.0 66-67 34.66980620108134 37.0 34.0 40.0 26.0 41.0 68-69 34.29752803651999 36.5 34.0 39.0 26.0 41.0 70-71 33.905319953915786 36.0 34.0 39.0 26.0 41.0 72-73 33.504319460613985 35.5 34.0 39.0 25.5 40.5 74-75 33.09463368781541 35.0 34.0 37.0 25.0 39.5 76-77 32.695664460807954 35.0 33.0 37.0 25.0 39.0 78-79 32.34049125172309 35.0 33.0 36.5 24.5 39.0 80-81 32.01961546483374 35.0 33.0 36.0 24.0 37.5 82-83 31.745387352254152 35.0 33.0 36.0 23.5 37.0 84-85 31.494543960985734 35.0 33.0 35.0 23.0 37.0 86-87 31.28265357222049 35.0 33.0 35.0 21.5 36.0 88-89 31.099892014596893 35.0 33.0 35.0 20.0 36.0 90-91 30.896317017951418 35.0 32.0 35.0 19.0 36.0 92-93 30.707049419872092 35.0 32.0 35.0 18.0 35.5 94-95 30.514397223273168 35.0 32.0 35.0 15.0 35.0 96-97 30.30433335919668 35.0 32.0 35.0 4.5 35.0 98-99 30.056655543648134 35.0 32.0 35.0 2.0 35.0 100-101 29.028122741035414 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38497660310511606 1101 2 0.39805928583979977 1101 3 0.36230664089607956 1101 4 0.3102505930543913 1101 5 0.32684671481330696 1101 6 0.30606035009466837 1101 7 0.3162018913781992 1101 8 0.26981826042936063 1101 9 0.3735668449686216 1101 10-11 0.38575061899741314 1101 12-13 0.3825997316953931 1101 14-15 0.5027146856840687 1101 16-17 0.43705168420220275 1101 18-19 0.4630400201764573 1101 20-21 0.4866689867067038 1101 22-23 0.5123599041664733 1101 24-25 0.49513205536631233 1101 26-27 0.43758234853748235 1101 28-29 0.4379395660854186 1101 30-31 0.4977942202730219 1101 32-33 0.5085751685548345 1101 34-35 0.4921174623984328 1101 36-37 0.46692736926443956 1101 38-39 0.5001346798410466 1101 40-41 0.4915463800404609 1101 42-43 0.44687870971752375 1101 44-45 0.36347597863468195 1101 46-47 0.41235263891248763 1101 48-49 0.3409513887127531 1101 50-51 0.331381640119055 1101 52-53 0.3430875820240473 1101 54-55 0.37928154922785495 1101 56-57 0.48534166734774686 1101 58-59 0.46091722825986636 1101 60-61 0.4141464605723115 1101 62-63 0.4171702362954832 1101 64-65 0.4205487058759516 1101 66-67 0.42525281590747 1101 68-69 0.3389140647499289 1101 70-71 0.3620912777106682 1101 72-73 0.3757604127149463 1101 74-75 0.3044651687292941 1101 76-77 0.30415375478309414 1101 78-79 0.2717335710762967 1101 80-81 0.28715611433415944 1101 82-83 0.26439513542462834 1101 84-85 0.29157285883799133 1101 86-87 0.2367690093557293 1101 88-89 0.25533749280305074 1101 90-91 0.19508078965001374 1101 92-93 0.1706204689683446 1101 94-95 0.201189226143331 1101 96-97 0.1919860720191977 1101 98-99 0.17025934396709275 1101 100-101 0.11555477562146166 1102 1 0.3030135657742292 1102 2 0.30273497600697397 1102 3 0.3283020830614447 1102 4 0.20845596505476038 1102 5 0.2818742329263628 1102 6 0.258787256559863 1102 7 0.26661782477302154 1102 8 0.2595297851446574 1102 9 0.2836026490185546 1102 10-11 0.3109202250204248 1102 12-13 0.31580997676546474 1102 14-15 0.4114462819080913 1102 16-17 0.4229421676102092 1102 18-19 0.38603503180926424 1102 20-21 0.39257834548591575 1102 22-23 0.4319991590587122 1102 24-25 0.3969794546354066 1102 26-27 0.3863760392727045 1102 28-29 0.3396910672518487 1102 30-31 0.3383130381977324 1102 32-33 0.30573501823948845 1102 34-35 0.3376611832361007 1102 36-37 0.28948951391848965 1102 38-39 0.29917117170406726 1102 40-41 0.27929719490678195 1102 42-43 0.232849882583821 1102 44-45 0.1852768103648259 1102 46-47 0.15951007756769542 1102 48-49 0.13438969127209077 1102 50-51 0.12747193172703675 1102 52-53 0.11920248279083978 1102 54-55 0.15767995558055503 1102 56-57 0.18864577400565707 1102 58-59 0.1450780908444287 1102 60-61 0.0947812208718517 1102 62-63 0.1122484914883799 1102 64-65 0.08910327065096624 1102 66-67 0.07323571593560274 1102 68-69 -0.010026210332171104 1102 70-71 0.017122311636249776 1102 72-73 0.01219751881187392 1102 74-75 -0.018859193442565925 1102 76-77 -0.08525527277301137 1102 78-79 -0.06566025551811094 1102 80-81 -0.07267178934994334 1102 82-83 -0.06509482895160801 1102 84-85 -0.06978734411542575 1102 86-87 -0.09974244958285539 1102 88-89 -0.09615130362140434 1102 90-91 -0.13032781581746988 1102 92-93 -0.1273186533330417 1102 94-95 -0.15465853281650865 1102 96-97 -0.11138051500381607 1102 98-99 -0.12161899754189776 1102 100-101 -0.1592029746867354 1103 1 0.2066107341486898 1103 2 0.18984803805653172 1103 3 0.15145300060383704 1103 4 0.16651656263938008 1103 5 0.19032822083192258 1103 6 0.21360572552067225 1103 7 0.20297156203705669 1103 8 0.2508424295904206 1103 9 0.29974125339811764 1103 10-11 0.2847153385679704 1103 12-13 0.2707450891953229 1103 14-15 0.352710638673301 1103 16-17 0.381509525958613 1103 18-19 0.40027744894607054 1103 20-21 0.37147458128245603 1103 22-23 0.4074686392741782 1103 24-25 0.3759471344619243 1103 26-27 0.3391800602759787 1103 28-29 0.3322286812774564 1103 30-31 0.29215903424047696 1103 32-33 0.29360458293525227 1103 34-35 0.2285408166610452 1103 36-37 0.2416237357623814 1103 38-39 0.2787948464508361 1103 40-41 0.18778638597363084 1103 42-43 0.0971528086813862 1103 44-45 0.07212622343236319 1103 46-47 0.0550713718878697 1103 48-49 0.07027692293013388 1103 50-51 0.009365950646824217 1103 52-53 0.010838186180500031 1103 54-55 -0.02568631919743325 1103 56-57 0.0033533625965773695 1103 58-59 0.0025582759279814127 1103 60-61 -0.02685171068605996 1103 62-63 -0.03157790930114146 1103 64-65 -0.05068378584510924 1103 66-67 -0.03582778457779057 1103 68-69 -0.08310825080498319 1103 70-71 -0.1663123243918534 1103 72-73 -0.16619717267276712 1103 74-75 -0.1926980308821129 1103 76-77 -0.19244192623930445 1103 78-79 -0.2263672942940218 1103 80-81 -0.2689611197572326 1103 82-83 -0.28600149307631284 1103 84-85 -0.3073763803957803 1103 86-87 -0.3151800134386491 1103 88-89 -0.30619383888329565 1103 90-91 -0.360805745246779 1103 92-93 -0.40745834567901795 1103 94-95 -0.39778084489266163 1103 96-97 -0.45399734858268204 1103 98-99 -0.5382982820629145 1103 100-101 -0.5866390945878663 1104 1 0.11165403914522898 1104 2 0.15354455134648504 1104 3 0.09608982237241293 1104 4 0.14461058037570496 1104 5 0.16789344676823248 1104 6 0.17274918804199046 1104 7 0.16324266862951475 1104 8 0.18149448434447635 1104 9 0.2460342695095079 1104 10-11 0.21971090962826878 1104 12-13 0.19455616420829358 1104 14-15 0.24391956255205116 1104 16-17 0.3194203872328103 1104 18-19 0.2928718311486591 1104 20-21 0.30138609288896845 1104 22-23 0.3067591100908871 1104 24-25 0.31067626525731384 1104 26-27 0.2563955742210311 1104 28-29 0.20854377721975936 1104 30-31 0.19103945894115526 1104 32-33 0.21670067367482915 1104 34-35 0.21468900237260158 1104 36-37 0.19410024833879902 1104 38-39 0.1849439113255542 1104 40-41 0.10330872586155948 1104 42-43 -0.04831662338818177 1104 44-45 -0.042484400447079906 1104 46-47 -0.024270955753884493 1104 48-49 -0.09261644996692553 1104 50-51 -0.09798979154803078 1104 52-53 -0.06924923914324665 1104 54-55 0.011226103591994274 1104 56-57 0.03422617943052586 1104 58-59 -0.012343505191715565 1104 60-61 -0.03934970686341899 1104 62-63 -0.02182469295448186 1104 64-65 -0.06769363495683933 1104 66-67 -0.11085436304659169 1104 68-69 -0.19315486025426765 1104 70-71 -0.21683412739678687 1104 72-73 -0.2376433350047975 1104 74-75 -0.24310162301581784 1104 76-77 -0.2813291520330381 1104 78-79 -0.2853207654461869 1104 80-81 -0.3002722760272434 1104 82-83 -0.37726981740029686 1104 84-85 -0.3644883963410699 1104 86-87 -0.37795610189819584 1104 88-89 -0.42194207850397447 1104 90-91 -0.46980421657939786 1104 92-93 -0.5322015320437359 1104 94-95 -0.5618945656139935 1104 96-97 -0.5842475191534504 1104 98-99 -0.6445525773856922 1104 100-101 -0.6573866879477137 1105 1 0.03731859037189622 1105 2 -0.023303251484520615 1105 3 0.0529306705975543 1105 4 0.04408695085924563 1105 5 0.0929160799098625 1105 6 0.09812809675219114 1105 7 0.11248540008702435 1105 8 0.12468480377419411 1105 9 0.1361955147692484 1105 10-11 0.10812960746603295 1105 12-13 0.1406970252190689 1105 14-15 0.19662863014410448 1105 16-17 0.24096402238011194 1105 18-19 0.19655201979907133 1105 20-21 0.1655164701166001 1105 22-23 0.21467044779888766 1105 24-25 0.15369721818683502 1105 26-27 0.07314356629652252 1105 28-29 0.12090798913226308 1105 30-31 0.13463536685192423 1105 32-33 0.08915032865663619 1105 34-35 0.007529159226834281 1105 36-37 0.054709358592653246 1105 38-39 0.042455547537038285 1105 40-41 0.030394176679735097 1105 42-43 -0.007156328871062101 1105 44-45 -0.07962414820431718 1105 46-47 -0.16817428910012922 1105 48-49 -0.16489992947397525 1105 50-51 -0.14194736694555843 1105 52-53 -0.11488256567480448 1105 54-55 -0.13016839497252874 1105 56-57 -0.1665585007200292 1105 58-59 -0.166800404500826 1105 60-61 -0.23307921273124776 1105 62-63 -0.21605992523314654 1105 64-65 -0.21687119523546983 1105 66-67 -0.23293947162325424 1105 68-69 -0.22328946233501767 1105 70-71 -0.2545067979266946 1105 72-73 -0.24768963554622303 1105 74-75 -0.26180999708450514 1105 76-77 -0.29686061543623055 1105 78-79 -0.30249417018100644 1105 80-81 -0.3250614746267644 1105 82-83 -0.3745472538692951 1105 84-85 -0.43603921533146917 1105 86-87 -0.4733095007008217 1105 88-89 -0.4957521004547871 1105 90-91 -0.5653140079153935 1105 92-93 -0.6435334068685776 1105 94-95 -0.6494413493832241 1105 96-97 -0.6570972899807828 1105 98-99 -0.7733821200175761 1105 100-101 -0.8462720342423147 1106 1 0.03495702135612788 1106 2 0.036064620406847325 1106 3 0.022483710557558823 1106 4 0.05466381829310052 1106 5 0.022365586980306773 1106 6 0.043014384581901766 1106 7 0.04447932547164868 1106 8 0.05404949690083072 1106 9 0.053312941794288804 1106 10-11 0.06781677240976336 1106 12-13 0.07838899688908185 1106 14-15 0.12213765446025349 1106 16-17 0.10428351877697395 1106 18-19 0.06046165728325548 1106 20-21 0.035567412734877735 1106 22-23 0.0638663931257426 1106 24-25 0.07748920721748931 1106 26-27 0.041529374699393884 1106 28-29 0.038395575473295196 1106 30-31 -0.013313777332989218 1106 32-33 -0.038485062206845555 1106 34-35 -0.06871996269466507 1106 36-37 -0.022616976769839425 1106 38-39 -0.025988079421466637 1106 40-41 -0.07451906434216227 1106 42-43 -0.21958367139160373 1106 44-45 -0.2552272997224847 1106 46-47 -0.2269604992892127 1106 48-49 -0.2946876173265096 1106 50-51 -0.35166112541087813 1106 52-53 -0.26587895369789294 1106 54-55 -0.28914249788573443 1106 56-57 -0.2735589062034194 1106 58-59 -0.31117418182171974 1106 60-61 -0.35407960535491156 1106 62-63 -0.4335076062218022 1106 64-65 -0.33657650244640536 1106 66-67 -0.3153780824829724 1106 68-69 -0.3617051153601878 1106 70-71 -0.43083788053921523 1106 72-73 -0.44710795375061707 1106 74-75 -0.4246294207435142 1106 76-77 -0.5107355617870439 1106 78-79 -0.4948823950076786 1106 80-81 -0.47664849602374915 1106 82-83 -0.4977242965973545 1106 84-85 -0.5378973459766918 1106 86-87 -0.633463363362111 1106 88-89 -0.5911045736221539 1106 90-91 -0.652273678191893 1106 92-93 -0.7188665341783071 1106 94-95 -0.7023676916578196 1106 96-97 -0.8458665454184384 1106 98-99 -0.9082491303069702 1106 100-101 -0.9684732745633582 1107 1 -0.07481823823101053 1107 2 -0.08883170210578584 1107 3 -0.04109402017857633 1107 4 -0.062204140949766895 1107 5 -0.14530680823627762 1107 6 -0.11129825735785204 1107 7 -0.07875761003278825 1107 8 -0.09513938361175889 1107 9 -0.08758416915406997 1107 10-11 -0.04660878194636808 1107 12-13 -0.07443081282664821 1107 14-15 -0.08082173540098836 1107 16-17 -0.030180651268366887 1107 18-19 -0.02533553221054774 1107 20-21 -0.03587669522134718 1107 22-23 -0.022489910876835495 1107 24-25 -0.0696039568122444 1107 26-27 -0.14006295913679878 1107 28-29 -0.15895437077555385 1107 30-31 -0.13964744918823868 1107 32-33 -0.12165555722613419 1107 34-35 -0.13469161123709483 1107 36-37 -0.15420101376533069 1107 38-39 -0.3003842866048032 1107 40-41 -0.3004294128743865 1107 42-43 -0.2847196071163509 1107 44-45 -0.2475061124972342 1107 46-47 -0.34320177534142715 1107 48-49 -0.2264745268893975 1107 50-51 -0.19875749345141713 1107 52-53 -0.2146246259246425 1107 54-55 -0.1991326403634588 1107 56-57 -0.13830687593989666 1107 58-59 -0.273861388606619 1107 60-61 -0.29377034538252644 1107 62-63 -0.35781378504153594 1107 64-65 -0.2993533031287754 1107 66-67 -0.2919790293153355 1107 68-69 -0.26454383213612687 1107 70-71 -0.2973161269663649 1107 72-73 -0.31663652979085555 1107 74-75 -0.30973988628062443 1107 76-77 -0.2729073591857798 1107 78-79 -0.34594977403400407 1107 80-81 -0.3907879527827731 1107 82-83 -0.3648680993762632 1107 84-85 -0.35368234390747944 1107 86-87 -0.3441687653998038 1107 88-89 -0.39212846924039013 1107 90-91 -0.42972985249430806 1107 92-93 -0.4911665956017224 1107 94-95 -0.5619874892632133 1107 96-97 -0.5869725190003336 1107 98-99 -0.6261092177217442 1107 100-101 -0.5903665020921309 1108 1 -0.28097009676951146 1108 2 -0.22690179568859037 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-0.5658687508033466 2308 40-41 -0.6297718981127289 2308 42-43 -0.7510347153924357 2308 44-45 -0.645285824803544 2308 46-47 -0.6427515978059617 2308 48-49 -0.44328887519651516 2308 50-51 -1.0351444899246047 2308 52-53 -1.120612877386634 2308 54-55 -0.8369631196615757 2308 56-57 -0.8770633292994603 2308 58-59 -1.12064780707842 2308 60-61 -1.1934553066379863 2308 62-63 -1.1110530008335289 2308 64-65 -1.2453785632357892 2308 66-67 -1.0527760620944093 2308 68-69 -0.8984460948567587 2308 70-71 -0.8265130370018596 2308 72-73 -0.8406206845053532 2308 74-75 -1.1113197593494988 2308 76-77 -0.9382387671460464 2308 78-79 -0.7965017335526774 2308 80-81 -0.866861783968119 2308 82-83 -0.8545639061761996 2308 84-85 -0.8125990451124068 2308 86-87 -0.8232820094518729 2308 88-89 -0.9091704626777357 2308 90-91 -0.9090714461558882 2308 92-93 -0.8327593073759729 2308 94-95 -0.8114804885051043 2308 96-97 -0.8817286510867994 2308 98-99 -0.937791366672986 2308 100-101 -0.5312440184792422 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 74.0 11 684.0 12 2536.0 13 5484.0 14 8739.0 15 12069.0 16 15884.0 17 24366.0 18 51039.0 19 138562.0 20 153980.0 21 109960.0 22 93946.0 23 87010.0 24 87509.0 25 96445.0 26 113332.0 27 134173.0 28 154954.0 29 177286.0 30 201535.0 31 235198.0 32 284428.0 33 355900.0 34 479643.0 35 723098.0 36 1292837.0 37 2550153.0 38 3061717.0 39 585686.0 40 329.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.33626737331348 31.20990487193539 21.537093841140965 19.91673391361017 2 21.937449588270095 26.37316568934058 33.91823991605224 17.771144806337087 3 21.24096094580456 27.840705139213846 23.925711089466635 26.992622825514957 4 28.860305796574004 23.86572036956129 26.949944786482522 20.324029047382187 5 21.942782680486935 31.46494302178229 22.939900378104753 23.652373919626022 6 21.866193407682687 23.741614119822376 25.582768342604385 28.809424129890548 7 29.002457805244514 23.69025460911892 22.92661055114027 24.380677034496294 8 31.69061043073505 23.7405499425371 23.115131516895943 21.453708109831904 9 21.277039505787045 26.71173236134607 23.391919744849783 28.619308388017107 10-11 28.429768913372854 23.592764052606046 23.28253736512057 24.694929668900524 12-13 26.447049781128467 23.488942885545082 23.53565262298822 26.52835471033823 14-15 26.330393335229186 23.52357366907279 23.592626134531873 26.553406861166152 16-17 26.315934182291745 27.127381845140956 23.49520258652446 23.061481386042836 18-19 22.82375511587076 23.576805596733248 27.157568107504204 26.441871179891795 20-21 22.540493636371078 28.90159554305731 23.864044455533257 24.693866365038357 22-23 20.733975966307415 26.996359674677066 29.30492137957937 22.96474297943615 24-25 20.73433633288832 23.541525263565887 32.98720938882184 22.736929014723955 26-27 20.70132942345974 23.562115097348805 34.89595104566814 20.84060443352331 28-29 20.690994465837072 23.446664322356003 35.078514535141345 20.78382667666558 30-31 20.71017397608732 23.3939796180221 35.06071420563283 20.835132200257757 32-33 20.71905026513758 23.42546450795724 35.07109708987094 20.78438813703424 34-35 20.734856862394064 23.507739784363757 35.01134843301933 20.746054920222846 36-37 20.75563800189277 23.507993375661428 34.903865763537596 20.832502858908207 38-39 20.92989547033367 23.619156843616228 34.287262784854825 21.163684901195282 40-41 22.020698210695393 24.585373779336063 31.06317662162292 22.33075138834562 42-43 22.05210962957189 24.835794908180663 31.249912688813495 21.862182773433943 44-45 22.263745095216294 25.49182641341145 30.46031959018084 21.78410890119142 46-47 21.991579701164454 26.055598245895645 30.271099774739746 21.68172227820015 48-49 21.980742009916575 26.43703070038535 29.712776267698448 21.869451021999623 50-51 22.02664179985972 26.623594652064536 29.346652379523412 22.003111168552326 52-53 22.085743927447247 26.618110951387326 29.253912344251326 22.042232776914105 54-55 22.22471414525999 26.447712496957454 29.092510885430183 22.23506247235237 56-57 22.274156794685982 26.365388012874853 29.076968860478214 22.28348633196095 58-59 22.298694246779995 26.422849246554208 28.99852360617367 22.279932900492124 60-61 22.32514967334513 26.39363261136014 28.88384684621877 22.397370869075957 62-63 22.435885542960143 26.452561693003666 28.687568714439838 22.42398404959635 64-65 22.49959703193051 26.600888812089284 28.446978633727582 22.452535522252624 66-67 22.550453410093535 26.597831282299957 28.23499188987691 22.616723417729602 68-69 22.577949515934254 26.68466927601731 28.104255564504903 22.63312564354353 70-71 22.62107783241904 26.846936563736477 27.946477287651543 22.585508316192936 72-73 22.63238711450119 26.83941335523888 27.847283049530564 22.680916480729373 74-75 22.678927560892088 26.83923312345601 27.812743984443898 22.669095331208 76-77 22.73934599442865 26.910508226244673 27.700323280277544 22.64982249904913 78-79 22.756331840426476 26.808736408174216 27.681927863078798 22.75300388832051 80-81 22.75843183037657 26.82865029315532 27.628987895608407 22.783929980859703 82-83 22.787346299789604 26.90015346385035 27.567327242385016 22.745172993975032 84-85 22.8120901979863 26.817201301533977 27.52735016553916 22.843358334940564 86-87 22.851731512653835 26.883788866549597 27.515557297614983 22.748922323181585 88-89 22.884086456944082 26.915937113923054 27.420539639387087 22.779436789745773 90-91 22.906043602835467 26.863316780147173 27.311818417899563 22.918821199117794 92-93 22.903680631755346 26.858860761943482 27.336425178082273 22.901033428218895 94-95 22.91277863682463 26.96653412885679 27.245395626519215 22.875291607799365 96-97 22.896526920362366 26.94581047600777 27.24804681313151 22.909615790498354 98-99 22.955558945689653 26.957127305363482 27.218977347809115 22.86833640113775 100-101 23.11799232345779 26.937813494806502 27.022860253840285 22.92133392789542 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9859.0 1 5525.0 2 892.0 3 497.5 4 360.5 5 300.0 6 261.5 7 243.5 8 250.5 9 256.5 10 267.0 11 298.0 12 451.5 13 807.0 14 1169.5 15 2340.0 16 39437.5 17 44551.0 18 13096.5 19 12124.0 20 11967.5 21 13234.0 22 15767.0 23 19505.5 24 24132.0 25 30063.0 26 37963.0 27 48306.0 28 60872.5 29 76366.0 30 96523.5 31 119826.0 32 144977.0 33 176002.0 34 207735.0 35 238933.5 36 274945.5 37 309612.0 38 338878.5 39 365594.5 40 386921.0 41 398937.5 42 408846.5 43 423211.5 44 437750.5 45 452304.5 46 457534.5 47 454834.5 48 453355.0 49 448076.0 50 435421.5 51 418957.5 52 405491.5 53 392572.5 54 370984.0 55 342156.0 56 311005.5 57 275934.5 58 241741.0 59 207012.0 60 171024.0 61 139973.0 62 113865.0 63 89025.5 64 67959.0 65 51377.5 66 37846.5 67 27373.0 68 19867.0 69 14668.5 70 11069.5 71 8602.0 72 6811.5 73 5067.0 74 3679.5 75 2709.0 76 1933.0 77 1367.5 78 895.5 79 535.5 80 285.0 81 132.5 82 73.5 83 41.0 84 20.5 85 10.5 86 8.0 87 3.5 88 2.0 89 1.5 90 1.0 91 2.0 92 3.5 93 2.0 94 1.0 95 0.5 96 0.0 97 0.5 98 2.0 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00113003841418773 2 0.00971655077396064 3 0.0033634214217556063 4 0.0032566461385252696 5 0.0059171302790144925 6 0.001521547786032298 7 6.584475799204097E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 4.53794953728931E-4 34-35 0.0 36-37 0.0 38-39 2.1355056646067344E-4 40-41 0.0 42-43 1.6906086511469978E-4 44-45 3.3812173022939956E-4 46-47 0.0 48-49 0.0 50-51 1.201221936341288E-4 52-53 5.116315654786968E-4 54-55 2.179995365952708E-4 56-57 5.02733625209502E-4 58-59 2.091015963260761E-4 60-61 0.0014726091145517272 62-63 0.0041864808966561185 64-65 6.451006695166176E-4 66-67 0.003919542688580277 68-69 0.0 70-71 0.0 72-73 0.0 74-75 5.783661174976572E-5 76-77 0.0014726091145517272 78-79 0.0036792983013120193 80-81 0.004324398970828636 82-83 0.0035992168388892663 84-85 0.0024780763649707312 86-87 0.002322362410259823 88-89 9.476306386692382E-4 90-91 0.0012056709064758853 92-93 0.0025047701857783154 94-95 1.779588053838945E-5 96-97 0.0 98-99 5.561212668246703E-4 100-101 2.891830587488286E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1238556E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.84416651600214 #Duplication Level Percentage of deduplicated Percentage of total 1 80.18380014887092 49.5890028829261 2 12.022221673491716 14.870085581354234 3 3.7112166203641093 6.88551295940258 4 1.565774095997455 3.873359756772373 5 0.7990173271045736 2.47072803133131 6 0.43056753876485504 1.5976854338255342 7 0.25888021546090284 1.120716180686381 8 0.168126469956199 0.8318113122975042 9 0.11970546241082707 0.6662776095189202 >10 0.6203938561670607 7.471649949805322 >50 0.08059766889689128 3.572820783160676 >100 0.03851598858489558 3.3365727870802377 >500 6.70442091488028E-4 0.3030738561686864 >1k 3.843696276863769E-4 0.5311040503768314 >5k 5.694320465035902E-5 0.26875128789128166 >10k+ 7.117900581294877E-5 2.6108475374020625 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 225369 2.005319900528146 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 33839 0.3010974007692803 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 15488 0.13781129888928792 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.423670443071156E-4 0.0 0.0 0.0 0.0 2 1.423670443071156E-4 0.0 0.0 0.0 0.0 3 1.6906086511469978E-4 0.0 0.0 8.897940269194726E-6 8.897940269194726E-6 4 1.779588053838945E-4 0.0 0.0 8.897940269194726E-6 8.897940269194726E-6 5 1.779588053838945E-4 0.0 0.0 8.897940269194726E-6 8.897940269194726E-6 6 1.8685674565308924E-4 0.0 8.897940269194726E-6 8.897940269194726E-6 1.779588053838945E-5 7 2.135505664606734E-4 0.0 8.897940269194726E-6 8.897940269194726E-6 1.779588053838945E-5 8 2.4024438726825758E-4 0.0 8.897940269194726E-6 8.897940269194726E-6 1.779588053838945E-5 9 2.5804026780664705E-4 0.0 8.897940269194726E-6 1.779588053838945E-5 1.779588053838945E-5 10-11 3.0697893928721806E-4 0.0 8.897940269194726E-6 3.1142790942181536E-5 3.1142790942181536E-5 12-13 3.781624614407758E-4 0.0 8.897940269194726E-6 3.55917610767789E-5 5.338764161516835E-5 14-15 5.02733625209502E-4 0.0 8.897940269194726E-6 3.55917610767789E-5 7.11835221535578E-5 16-17 5.961619980360466E-4 0.0 8.897940269194726E-6 3.55917610767789E-5 7.11835221535578E-5 18-19 6.762434604587991E-4 0.0 8.897940269194726E-6 4.448970134597363E-5 7.11835221535578E-5 20-21 8.008146242275253E-4 0.0 8.897940269194726E-6 4.448970134597363E-5 8.008146242275254E-5 22-23 9.120388775924594E-4 0.0 8.897940269194726E-6 4.448970134597363E-5 8.897940269194727E-5 24-25 9.965693101498094E-4 8.897940269194726E-6 8.897940269194726E-6 4.448970134597363E-5 9.787734296114199E-5 26-27 0.0010988956232455486 8.897940269194726E-6 8.897940269194726E-6 4.448970134597363E-5 1.1122425336493409E-4 28-29 0.0011789770856683013 8.897940269194726E-6 8.897940269194726E-6 4.448970134597363E-5 1.46816014441713E-4 30-31 0.0012902013390332352 8.897940269194726E-6 8.897940269194726E-6 4.448970134597363E-5 1.6906086511469978E-4 32-33 0.001459262204147935 8.897940269194726E-6 8.897940269194726E-6 4.448970134597363E-5 2.046526261914787E-4 34-35 0.0016995065914161924 8.897940269194726E-6 1.3346910403792088E-5 4.448970134597363E-5 2.179995365952708E-4 36-37 0.00300305484085322 8.897940269194726E-6 1.779588053838945E-5 4.448970134597363E-5 2.535912976720497E-4 38-39 0.0031810136462371145 8.897940269194726E-6 1.779588053838945E-5 4.448970134597363E-5 2.802851184796339E-4 40-41 0.003381217302293996 8.897940269194726E-6 1.779588053838945E-5 5.783661174976572E-5 2.980809990180233E-4 42-43 0.0035235843466011113 8.897940269194726E-6 1.779588053838945E-5 8.008146242275254E-5 3.025299691526207E-4 44-45 0.0036437065402352403 8.897940269194726E-6 1.779588053838945E-5 8.897940269194726E-5 3.3367276009480223E-4 46-47 0.003915093718445679 8.897940269194726E-6 1.779588053838945E-5 9.787734296114199E-5 3.559176107677891E-4 48-49 0.00430215412015565 1.3346910403792088E-5 1.779588053838945E-5 9.787734296114199E-5 3.8261143157537324E-4 50-51 0.00464027585038505 2.6693820807584177E-5 1.779588053838945E-5 9.787734296114199E-5 4.0040731211376265E-4 52-53 0.0051563563859983435 2.6693820807584177E-5 1.779588053838945E-5 1.0232631309573935E-4 4.1375422251755474E-4 54-55 0.0056768858917462355 2.6693820807584177E-5 1.779588053838945E-5 1.2457116376872617E-4 4.226521627867495E-4 56-57 0.006188517457224932 2.6693820807584177E-5 1.779588053838945E-5 1.2457116376872617E-4 4.448970134597363E-4 58-59 0.007100556334817392 2.6693820807584177E-5 1.779588053838945E-5 1.2457116376872617E-4 4.6269289399812575E-4 60-61 0.008025942122813642 2.6693820807584177E-5 1.779588053838945E-5 1.2457116376872617E-4 4.982846550749046E-4 62-63 0.009107041865520802 2.6693820807584177E-5 1.779588053838945E-5 1.2902013390332352E-4 5.205295057478915E-4 64-65 0.0104595287864384 2.6693820807584177E-5 1.779588053838945E-5 1.3791807417251823E-4 5.561212668246703E-4 66-67 0.012243565810411944 2.6693820807584177E-5 1.779588053838945E-5 1.423670443071156E-4 5.65019207093865E-4 68-69 0.014330132803538106 3.55917610767789E-5 1.779588053838945E-5 1.46816014441713E-4 5.917130279014493E-4 70-71 0.017524493360179014 3.55917610767789E-5 1.779588053838945E-5 1.6016292484550507E-4 6.050599383052414E-4 72-73 0.021390648407144122 3.55917610767789E-5 1.779588053838945E-5 1.6016292484550507E-4 6.139578785744361E-4 74-75 0.026022026317259975 3.55917610767789E-5 1.779588053838945E-5 1.6016292484550507E-4 6.362027292474229E-4 76-77 0.031823483372774936 3.55917610767789E-5 1.779588053838945E-5 1.779588053838945E-4 6.451006695166176E-4 78-79 0.03962252801872412 3.55917610767789E-5 1.779588053838945E-5 1.779588053838945E-4 6.584475799204097E-4 80-81 0.04930348703160797 3.55917610767789E-5 1.779588053838945E-5 1.779588053838945E-4 6.717944903242018E-4 82-83 0.06115554347017535 3.55917610767789E-5 1.779588053838945E-5 1.779588053838945E-4 7.029372812663833E-4 84-85 0.07756779429670502 3.55917610767789E-5 1.779588053838945E-5 1.779588053838945E-4 7.029372812663833E-4 86-87 0.09814428116921783 4.448970134597363E-5 1.779588053838945E-5 1.779588053838945E-4 7.11835221535578E-4 88-89 0.12411291984486264 4.448970134597363E-5 1.779588053838945E-5 1.913057157876866E-4 7.11835221535578E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 81100 0.0 93.91341 1 TCGACGG 82680 0.0 92.14172 2 CGACGGC 83855 0.0 90.75673 3 ACGGCGC 84830 0.0 89.75161 5 GACGGCG 85150 0.0 89.437035 4 CGGCGCG 85345 0.0 89.181404 6 TATCTCG 43565 0.0 88.4444 2 ATATCTC 44295 0.0 87.11549 1 ATCTCGA 46905 0.0 82.0524 3 TCTCGAG 47570 0.0 79.43673 4 CTCGAGG 48840 0.0 77.4194 5 TCGAGGG 49180 0.0 76.87869 6 CGAGGGC 50160 0.0 75.18629 7 AGGGCGC 50300 0.0 75.09945 9 GAGGGCG 51675 0.0 73.12872 8 CGCGCCG 134270 0.0 57.378025 9 GCGCGCC 134935 0.0 57.095253 8 GGCGCGC 135060 0.0 57.077827 7 CTCGACG 1620 0.0 41.051155 5 TCCATAT 94980 0.0 40.081604 18-19 >>END_MODULE