FastQCFastQC Report
Tue 31 May 2016
SRR1051284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11238556
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA605150.5384588553903188No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT138260.12302292216188628No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT118670.1055918571745338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG57200.082.1211551
TCGACGG62750.074.564472
CGACGGC74750.062.5295943
ACGGCGC85600.054.601875
GACGGCG87700.053.2414444
CGGCGCG90600.051.6395766
TATCTCG43300.050.9025462
ATATCTC56700.039.3263741
ATCTCGA88900.038.2382851
TCTCGAG103400.032.617242
TCGAGGG117150.029.0718564
CTCGAGG118100.028.6372813
CGCGCCG215450.028.4612779
GCGCGCC222900.027.5952518
GGCGCGC222600.027.5897667
CGAGGGC128350.026.608435
GGGCGCG127950.026.5424128
AGGGCGC132800.025.6445887
GAGGGCG145850.023.5779886
TCCATAT145800.017.05429518-19
Produced by FastQC (version 0.11.5)