FastQCFastQC Report
Tue 31 May 2016
SRR1051283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6609294
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT578740.8756457194974229No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA88810.13437138671694737No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT69480.10512469259197729No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA66450.10054023924491785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG695850.094.3166351
TCGACGG700550.093.690642
CGACGGC710400.092.351463
GACGGCG717550.091.4510964
ACGGCGC718150.091.341625
CGGCGCG719300.091.162576
TATCTCG201900.087.517882
ATATCTC218100.081.213251
ATCTCGA219050.080.687563
TCTCGAG223650.078.5819854
CTCGAGG234750.074.906755
TCGAGGG237600.073.90836
CGAGGGC239900.073.1997157
AGGGCGC241400.073.05979
GAGGGCG249600.070.526298
CGCGCCG954250.069.403969
GGCGCGC957250.069.186457
GCGCGCC957600.069.166128
TCCATAT765050.042.914518-19
GATCCAT775800.042.371916-17
Produced by FastQC (version 0.11.5)