##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1051283_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6609294
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08263893238824	33.0	31.0	34.0	30.0	34.0
2	32.23827597924983	34.0	31.0	34.0	30.0	34.0
3	32.29738471310249	34.0	31.0	34.0	30.0	34.0
4	35.941987298492094	37.0	35.0	37.0	35.0	37.0
5	35.827003307766304	37.0	35.0	37.0	35.0	37.0
6	35.92469301562315	37.0	35.0	37.0	35.0	37.0
7	35.87808834044907	37.0	35.0	37.0	35.0	37.0
8	35.94345871132378	37.0	35.0	37.0	35.0	37.0
9	37.62166730667451	39.0	38.0	39.0	35.0	39.0
10-11	37.592444215675684	39.0	37.5	39.0	35.0	39.0
12-13	37.584403266067454	39.0	37.0	39.0	35.0	39.0
14-15	38.92867861529537	40.0	38.5	41.0	35.5	41.0
16-17	38.91912267785334	40.5	38.5	41.0	35.5	41.0
18-19	38.81848333573903	40.0	38.5	41.0	35.0	41.0
20-21	38.91860862900032	40.0	39.0	41.0	35.0	41.0
22-23	38.821666731726566	40.0	38.5	41.0	34.5	41.0
24-25	38.697493786779646	40.0	38.0	41.0	34.0	41.0
26-27	38.71041838961922	40.0	38.0	41.0	34.0	41.0
28-29	38.43631626918094	40.0	38.0	41.0	34.0	41.0
30-31	38.0332021090301	40.0	36.5	41.0	34.0	41.0
32-33	38.02531450409076	40.0	35.5	41.0	34.0	41.0
34-35	37.84519004601702	40.0	35.0	41.0	34.0	41.0
36-37	37.7713278453039	40.0	35.0	41.0	34.0	41.0
38-39	37.59718950314512	40.0	35.0	41.0	33.0	41.0
40-41	36.70524839718131	40.0	35.0	41.0	29.5	41.0
42-43	36.64764799387045	40.0	35.0	41.0	29.0	41.0
44-45	36.7003696612679	40.0	35.0	41.0	29.5	41.0
46-47	36.95457063946618	40.0	35.0	41.0	30.0	41.0
48-49	36.91959837162638	40.0	35.0	41.0	30.0	41.0
50-51	36.929753011441164	40.0	35.0	41.0	30.0	41.0
52-53	36.79568180807209	40.0	35.0	41.0	30.0	41.0
54-55	36.57566965548817	39.5	35.0	41.0	29.5	41.0
56-57	36.36065697788599	39.0	35.0	41.0	29.0	41.0
58-59	36.10616232232974	39.0	35.0	41.0	29.0	41.0
60-61	35.84712414064195	39.0	35.0	40.5	29.0	41.0
62-63	35.55930996563325	38.0	34.0	40.0	29.0	41.0
64-65	35.25147368236304	37.5	34.0	40.0	28.0	41.0
66-67	34.875176380412185	37.0	34.0	40.0	28.0	41.0
68-69	34.546424096128874	36.5	34.0	39.0	27.5	41.0
70-71	34.1478116270815	36.0	34.0	39.0	26.5	41.0
72-73	33.76120490327712	35.5	34.0	38.5	26.0	40.0
74-75	33.38375520895273	35.0	33.0	37.0	26.0	39.5
76-77	33.02328584565916	35.0	33.0	37.0	26.0	39.0
78-79	32.72000473878148	35.0	33.0	36.5	26.0	39.0
80-81	32.40396969782249	35.0	33.0	36.0	25.0	37.0
82-83	32.17174557524601	35.0	33.0	36.0	25.0	37.0
84-85	31.94820119970454	35.0	33.0	35.0	25.0	37.0
86-87	31.736807970715176	35.0	33.0	35.0	25.0	36.0
88-89	31.547030363606158	35.0	33.0	35.0	25.0	36.0
90-91	31.363788174652242	35.0	32.5	35.0	24.5	36.0
92-93	31.149871453743774	35.0	32.0	35.0	24.0	35.0
94-95	30.979397496918732	35.0	32.0	35.0	23.5	35.0
96-97	30.75849243504677	35.0	32.0	35.0	21.0	35.0
98-99	30.49984733619052	34.5	32.0	35.0	17.5	35.0
100-101	28.88978588333338	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	21.0
11	337.0
12	1424.0
13	3065.0
14	4820.0
15	6427.0
16	8179.0
17	11713.0
18	23285.0
19	63111.0
20	66872.0
21	50292.0
22	44908.0
23	43870.0
24	46918.0
25	53642.0
26	64400.0
27	78595.0
28	95566.0
29	113784.0
30	130739.0
31	156236.0
32	195665.0
33	251552.0
34	331957.0
35	448653.0
36	737275.0
37	1509870.0
38	1789511.0
39	276548.0
40	59.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.660717165857655	28.86563980963776	21.451988669289037	20.02165435521555
2	21.10561279313645	25.26905294271975	35.47120161396966	18.154132650174134
3	20.486530028774634	26.37599416821222	22.881672384372674	30.255803418640475
4	31.29358445849133	23.280202091176456	25.51743045475054	19.908782995581674
5	21.19156751084155	34.00484227210955	22.478936479448485	22.324653737600418
6	21.214035871304862	23.137009792573913	24.54517835036541	31.103775985755817
7	31.453843632920552	23.217154509997588	22.40001730895917	22.92898454812269
8	33.54983149486163	23.294817873134406	22.588388411833396	20.566962220170566
9	20.526095525482752	25.63653546051969	22.852682903801828	30.984686110195735
10-11	28.54558293215584	23.110917747039245	22.792101546700753	25.551397774104164
12-13	26.943641786853483	23.024516688166692	22.97957240213554	27.052269122844287
14-15	26.85101464695019	23.13475539142305	23.000550437005828	27.013679524620937
16-17	26.79177987845601	28.30060971716495	22.981145943878424	21.926464460500622
18-19	21.660271127294383	23.120154739674163	28.276159299313967	26.943414833717487
20-21	21.394698132659855	29.721169916181665	23.332794697890577	25.551337253267896
22-23	20.00840785717809	28.087795761544278	30.10578739574908	21.79800898552856
24-25	20.028863899835596	22.966091385857553	35.425939593548115	21.579105120758737
26-27	20.003997401235292	22.94966754391619	36.937999731892695	20.108335322955824
28-29	20.023174941226703	22.924922086988413	37.00353623246295	20.048366739321928
30-31	20.020232115563328	22.85337132831434	37.02866750972191	20.097729046400417
32-33	20.041187455120017	22.855822422183063	37.01581439712018	20.087175725576742
34-35	20.03802524142518	22.941391319556974	36.981945424125485	20.038638014892364
36-37	20.08070756120094	22.945234392659792	36.83466796907506	20.139390077064206
38-39	20.401316691313777	23.127038984799285	35.8724396281963	20.599204695690645
40-41	22.151412843792393	24.50211021025846	30.697953518182125	22.64852342776702
42-43	22.073151837397457	24.976722173351646	31.152238045394864	21.797887943856033
44-45	22.42865122961696	25.913357765594935	30.00002723437632	21.657963770411786
46-47	22.007766638917865	26.77666479959887	29.617346421569383	21.598222139913887
48-49	22.025915324692775	27.375049437958122	28.72125978962352	21.877775447725583
50-51	22.143961215827286	27.50821797305431	28.28224920846311	22.06557160265529
52-53	22.198301361688554	27.35572816098058	28.277778231684046	22.16819224564681
54-55	22.278340167648768	27.035398939735472	28.43787097381354	22.248389918802218
56-57	22.269239346895446	26.81884025737091	28.681974201783127	22.22994619395052
58-59	22.246392731205482	26.7842601645501	28.848890668201477	22.120456436042943
60-61	22.291427798491036	26.778071909042023	28.729339321264874	22.201160971202068
62-63	22.40545661911847	26.85525867059326	28.49569863286457	22.2435860774237
64-65	22.49304539940272	26.93188561440904	28.22802253916984	22.347046447018396
66-67	22.573840715816242	26.97713250462152	27.91505416463544	22.533972614926796
68-69	22.53439626078065	27.117442498396954	27.744506448041196	22.603654792781196
70-71	22.60305714952308	27.213360458772147	27.621429762392168	22.5621526293126
72-73	22.624042749497907	27.224609769212872	27.46176823122107	22.689579250068164
74-75	22.701856296285985	27.242516547433553	27.37385655289868	22.68177060338178
76-77	22.731476977319044	27.29864809392728	27.28829874750212	22.681576181251554
78-79	22.762296922531263	27.210663405564212	27.227209505859452	22.79983016604507
80-81	22.789321539426798	27.201853155241473	27.167263102968274	22.84156220236346
82-83	22.787774644426495	27.285434112723784	27.118424034925958	22.808367207923762
84-85	22.775116402944565	27.190692938991745	27.17114461275288	22.86304604531081
86-87	22.81501664387894	27.238125297266162	27.142750473148247	22.80410758570665
88-89	22.869696549255274	27.215200119953803	27.03063951203858	22.884463818752344
90-91	22.905494096147002	27.193748855303646	26.885576360712516	23.015180687836832
92-93	22.84839456695169	27.20904351094213	26.937040074567754	23.005521847538425
94-95	22.852864466310624	27.286106503962447	26.92727090064385	22.933758129083078
96-97	22.908581461196913	27.217907086596544	26.925455275555905	22.94805617665064
98-99	22.965781216571695	27.224360120763276	26.88924868526048	22.920609977404546
100-101	23.054724150567367	27.17556519652477	26.739376399355212	23.03033425355265
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2357.0
1	1301.5
2	175.0
3	93.0
4	67.0
5	43.5
6	31.0
7	25.0
8	23.5
9	20.5
10	22.5
11	29.5
12	37.0
13	75.5
14	139.0
15	326.5
16	5646.0
17	6389.0
18	2018.5
19	2239.5
20	2942.0
21	4089.0
22	5506.0
23	7635.0
24	10567.5
25	14368.5
26	19407.0
27	25966.0
28	34754.0
29	46002.5
30	59855.0
31	76113.5
32	94263.5
33	115262.5
34	136571.5
35	157089.5
36	178477.5
37	200031.0
38	217962.0
39	231234.0
40	239886.5
41	243239.0
42	248686.0
43	260273.5
44	274654.5
45	286279.0
46	285815.0
47	277264.0
48	269784.0
49	261999.5
50	251447.0
51	238789.5
52	226138.0
53	215057.0
54	201839.0
55	185937.5
56	168353.5
57	148836.0
58	130999.5
59	111899.0
60	91841.5
61	75803.0
62	62280.0
63	48617.5
64	37195.5
65	28287.5
66	20708.0
67	14954.5
68	11028.5
69	8304.0
70	6329.5
71	4981.0
72	3954.5
73	2963.5
74	2137.0
75	1599.0
76	1162.0
77	784.0
78	522.5
79	307.0
80	164.0
81	85.0
82	41.5
83	24.0
84	11.5
85	5.0
86	2.0
87	1.5
88	0.5
89	1.0
90	1.5
91	1.0
92	2.0
93	4.0
94	3.0
95	1.5
96	1.5
97	2.0
98	2.5
99	3.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0016945834154147175
76-77	0.002738567840982713
78-79	0.007353281606174578
80-81	0.006922070647787796
82-83	0.0016945834154147175
84-85	4.917317946515921E-4
86-87	0.0016264974746168046
88-89	0.0012558073524948352
90-91	1.286067770627241E-4
92-93	3.7825522665507084E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6609294.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.44013429342212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.57140274115199	54.0348368417723
2	10.671264689984802	13.966579887865183
3	3.104023319558174	6.093831086454025
4	1.3085898139637078	3.4253717264315724
5	0.6407739034550504	2.09661651469094
6	0.3802557432131424	1.4930392141027846
7	0.24016367611898207	1.1001440252339687
8	0.16576818542771427	0.8678313852773218
9	0.11571367686984417	0.681508669855343
>10	0.7123478426901738	9.232231976352212
>50	0.07245385769434841	3.1473700521736174
>100	0.016152649031978262	1.6036297464679137
>500	4.638004694092571E-4	0.21760021912521418
>1k	4.86966955231362E-4	0.6625035575909931
>5k	1.1594451278996358E-4	0.4986758870204339
>10k+	2.3188902557992718E-5	0.8782292095861661
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	57874	0.8756457194974229	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	8881	0.13437138671694737	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT	6948	0.10512469259197729	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	6645	0.10054023924491785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.361718815958255E-4	0.0	0.0	0.0	1.5130209066202836E-5
2	1.361718815958255E-4	0.0	0.0	0.0	1.5130209066202836E-5
3	1.5130209066202836E-4	0.0	0.0	0.0	3.026041813240567E-5
4	1.5130209066202836E-4	1.5130209066202836E-5	0.0	0.0	3.026041813240567E-5
5	1.664322997282312E-4	1.5130209066202836E-5	0.0	0.0	3.026041813240567E-5
6	1.8156250879443401E-4	1.5130209066202836E-5	0.0	3.026041813240567E-5	4.5390627198608504E-5
7	1.9669271786063687E-4	1.5130209066202836E-5	0.0	3.026041813240567E-5	4.5390627198608504E-5
8	1.9669271786063687E-4	1.5130209066202836E-5	0.0	4.5390627198608504E-5	4.5390627198608504E-5
9	2.118229269268397E-4	1.5130209066202836E-5	0.0	4.5390627198608504E-5	4.5390627198608504E-5
10-11	2.118229269268397E-4	3.782552266550709E-5	0.0	4.5390627198608504E-5	5.295573173170992E-5
12-13	2.193880314599411E-4	4.5390627198608504E-5	0.0	4.5390627198608504E-5	6.052083626481134E-5
14-15	2.3451824052614394E-4	4.5390627198608504E-5	0.0	4.5390627198608504E-5	6.052083626481134E-5
16-17	2.4964844959234674E-4	4.5390627198608504E-5	0.0	6.052083626481134E-5	6.052083626481134E-5
18-19	2.950390767909553E-4	4.5390627198608504E-5	0.0	6.052083626481134E-5	6.052083626481134E-5
20-21	3.555599130557666E-4	5.295573173170992E-5	0.0	6.052083626481134E-5	6.052083626481134E-5
22-23	4.0095054025437516E-4	6.052083626481134E-5	0.0	6.052083626481134E-5	6.052083626481134E-5
24-25	4.766015855853893E-4	6.052083626481134E-5	0.0	6.052083626481134E-5	6.052083626481134E-5
26-27	6.279036762474176E-4	6.052083626481134E-5	0.0	6.052083626481134E-5	6.808594079791276E-5
28-29	7.111198261115332E-4	6.052083626481134E-5	0.0	6.052083626481134E-5	8.32161498641156E-5
30-31	7.489453487770403E-4	6.052083626481134E-5	0.0	6.052083626481134E-5	9.078125439721701E-5
32-33	7.716406623763446E-4	6.052083626481134E-5	0.0	6.808594079791276E-5	9.078125439721701E-5
34-35	8.321614986411559E-4	6.808594079791276E-5	0.0	7.565104533101418E-5	9.078125439721701E-5
36-37	9.078125439721701E-4	7.565104533101418E-5	0.0	9.078125439721701E-5	9.078125439721701E-5
38-39	9.456380666376772E-4	9.078125439721701E-5	0.0	9.078125439721701E-5	9.078125439721701E-5
40-41	9.532031711707786E-4	1.0591146346341985E-4	0.0	9.078125439721701E-5	9.834635893031843E-5
42-43	9.834635893031843E-4	1.1347656799652126E-4	0.0	9.834635893031843E-5	1.0591146346341985E-4
44-45	0.0010061589029024885	1.2104167252962269E-4	0.0	1.0591146346341985E-4	1.0591146346341985E-4
46-47	0.00101372400743559	1.2104167252962269E-4	0.0	1.0591146346341985E-4	1.0591146346341985E-4
48-49	0.0010515495301010968	1.2104167252962269E-4	0.0	1.0591146346341985E-4	1.0591146346341985E-4
50-51	0.0011196354708990098	1.2104167252962269E-4	0.0	1.0591146346341985E-4	1.0591146346341985E-4
52-53	0.0013087630842265451	1.2104167252962269E-4	0.0	1.2860677706272408E-4	1.2104167252962269E-4
54-55	0.001649192788216109	1.2104167252962269E-4	0.0	1.664322997282312E-4	1.2104167252962269E-4
56-57	0.001959362074073267	1.361718815958255E-4	0.0	1.664322997282312E-4	1.361718815958255E-4
58-59	0.002587265750320685	1.4373698612892694E-4	0.0	1.664322997282312E-4	1.5130209066202836E-4
60-61	0.003275690262832914	1.5130209066202836E-4	0.0	1.8156250879443401E-4	1.664322997282312E-4
62-63	0.004198633015871287	1.5130209066202836E-4	0.0	1.9669271786063687E-4	1.8156250879443401E-4
64-65	0.0052426174414392825	1.5130209066202836E-4	0.0	1.9669271786063687E-4	1.8912761332753544E-4
66-67	0.006763203452592667	1.5130209066202836E-4	0.0	1.9669271786063687E-4	2.042578223937383E-4
68-69	0.00880578167653005	1.5130209066202836E-4	0.0	1.9669271786063687E-4	2.118229269268397E-4
70-71	0.011839388594303718	1.5130209066202836E-4	0.0	1.9669271786063687E-4	2.118229269268397E-4
72-73	0.015879154414979876	1.5130209066202836E-4	0.0	1.9669271786063687E-4	2.193880314599411E-4
74-75	0.021204988006283274	1.664322997282312E-4	0.0	1.9669271786063687E-4	2.3451824052614394E-4
76-77	0.027552110709555364	1.664322997282312E-4	0.0	1.9669271786063687E-4	2.5721355412544817E-4
78-79	0.03607041841382756	1.739974042613326E-4	0.0	1.9669271786063687E-4	2.72343763191651E-4
80-81	0.04731216375001626	1.8156250879443401E-4	0.0	1.9669271786063687E-4	2.72343763191651E-4
82-83	0.062298635830090175	1.8156250879443401E-4	0.0	1.9669271786063687E-4	2.72343763191651E-4
84-85	0.08228564200654412	1.8156250879443401E-4	0.0	2.042578223937383E-4	2.950390767909553E-4
86-87	0.10704622914338506	1.8156250879443401E-4	0.0	2.118229269268397E-4	3.026041813240567E-4
88-89	0.13922818382719848	1.8156250879443401E-4	0.0	2.118229269268397E-4	3.026041813240567E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	69585	0.0	94.316635	1
TCGACGG	70055	0.0	93.69064	2
CGACGGC	71040	0.0	92.35146	3
GACGGCG	71755	0.0	91.451096	4
ACGGCGC	71815	0.0	91.34162	5
CGGCGCG	71930	0.0	91.16257	6
TATCTCG	20190	0.0	87.51788	2
ATATCTC	21810	0.0	81.21325	1
ATCTCGA	21905	0.0	80.68756	3
TCTCGAG	22365	0.0	78.581985	4
CTCGAGG	23475	0.0	74.90675	5
TCGAGGG	23760	0.0	73.9083	6
CGAGGGC	23990	0.0	73.199715	7
AGGGCGC	24140	0.0	73.0597	9
GAGGGCG	24960	0.0	70.52629	8
CGCGCCG	95425	0.0	69.40396	9
GGCGCGC	95725	0.0	69.18645	7
GCGCGCC	95760	0.0	69.16612	8
TCCATAT	76505	0.0	42.9145	18-19
GATCCAT	77580	0.0	42.3719	16-17
>>END_MODULE