FastQCFastQC Report
Tue 31 May 2016
SRR1051283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12655055
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA318280.25150424079547656No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT136890.10817021340484098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG59150.082.881561
TCGACGG64950.075.694692
CGACGGC83550.058.728813
ACGGCGC92450.053.0222435
GACGGCG96400.050.800784
TATCTCG39000.048.8409352
CGGCGCG100150.048.8029756
ATCTCGA85450.040.2884451
TCTCGAG98200.034.8277322
CGCGCCG226100.030.2722939
GGCGCGC233900.029.4049367
GCGCGCC235700.029.1199198
TCGAGGG121750.028.441164
CTCGAGG125400.027.5002353
CGAGGGC132500.026.3844015
GGGCGCG132300.026.136868
AGGGCGC137050.025.2309887
ATATCTC79350.024.3294961
GAGGGCG154450.022.573046
GCCGGAT230950.017.41002812-13
Produced by FastQC (version 0.11.5)