##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1051283_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12655055
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.522302352696215	34.0	31.0	34.0	31.0	34.0
2	32.77581962306762	34.0	31.0	34.0	31.0	34.0
3	32.85248013540834	34.0	31.0	34.0	31.0	34.0
4	36.31317682933816	37.0	37.0	37.0	35.0	37.0
5	36.18066954272423	37.0	35.0	37.0	35.0	37.0
6	36.137677868646165	37.0	36.0	37.0	35.0	37.0
7	36.10741083306236	37.0	36.0	37.0	35.0	37.0
8	36.086345258870864	37.0	36.0	37.0	35.0	37.0
9	37.79725927702408	39.0	38.0	39.0	35.0	39.0
10-11	37.7827431014721	39.0	38.0	39.0	35.0	39.0
12-13	37.78005429450919	39.0	38.0	39.0	35.0	39.0
14-15	39.221892832547944	40.0	39.0	41.0	36.0	41.0
16-17	39.226095500967794	40.0	39.0	41.0	36.0	41.0
18-19	39.29351595074063	41.0	39.0	41.0	36.0	41.0
20-21	39.34337460406138	41.0	39.0	41.0	36.0	41.0
22-23	39.315541813133166	41.0	39.0	41.0	36.0	41.0
24-25	39.27204061934144	41.0	39.0	41.0	36.0	41.0
26-27	39.15604266437404	40.0	39.0	41.0	36.0	41.0
28-29	39.02544809169142	40.0	38.5	41.0	35.5	41.0
30-31	38.929258782360094	40.0	38.0	41.0	35.0	41.0
32-33	38.91445374990468	40.0	38.0	41.0	35.0	41.0
34-35	38.858893422430796	40.0	38.0	41.0	35.0	41.0
36-37	38.80332183463446	40.0	38.0	41.0	35.0	41.0
38-39	38.730541945491346	40.0	38.0	41.0	35.0	41.0
40-41	38.743927150059804	40.0	38.0	41.0	35.0	41.0
42-43	38.73242565915359	40.0	38.0	41.0	35.0	41.0
44-45	38.67461330669839	40.0	38.0	41.0	35.0	41.0
46-47	38.57609307110874	40.0	38.0	41.0	34.5	41.0
48-49	38.51824756984462	40.0	38.0	41.0	34.0	41.0
50-51	38.4830292717021	40.0	38.0	41.0	34.0	41.0
52-53	38.33829596157425	40.0	38.0	41.0	34.0	41.0
54-55	38.19219738673597	40.0	37.0	41.0	34.0	41.0
56-57	38.04925604827478	40.0	37.0	41.0	34.0	41.0
58-59	37.84292743097521	40.0	37.0	41.0	33.5	41.0
60-61	37.577555648711126	39.0	36.0	41.0	33.0	41.0
62-63	37.28206380770372	39.0	35.5	41.0	33.0	41.0
64-65	36.9755732393103	39.0	35.0	40.5	32.5	41.0
66-67	36.59898633391953	38.0	35.0	40.0	32.0	41.0
68-69	36.208122090342556	37.0	35.0	40.0	32.0	41.0
70-71	35.86525874443059	37.0	35.0	39.0	32.0	41.0
72-73	35.46365890942394	36.0	35.0	39.0	31.0	40.5
74-75	35.078746319158626	36.0	35.0	38.5	31.0	39.5
76-77	34.46373638834442	35.0	34.0	37.0	30.5	39.0
78-79	34.36706454456342	35.0	34.0	37.0	31.0	39.0
80-81	34.11800687551338	35.0	34.0	36.0	31.0	38.0
82-83	33.85455057287384	35.0	34.0	36.0	31.0	37.0
84-85	33.611608009605646	35.0	34.0	35.5	30.0	37.0
86-87	33.41010070679266	35.0	34.0	35.0	30.0	36.0
88-89	33.22447764944522	35.0	34.0	35.0	30.0	36.0
90-91	33.075321047597185	35.0	34.0	35.0	30.0	36.0
92-93	32.93263470605224	35.0	34.0	35.0	30.0	36.0
94-95	32.795163513710534	35.0	34.0	35.0	30.0	35.0
96-97	32.65806241063354	35.0	34.0	35.0	29.0	35.0
98-99	32.48523783578973	35.0	34.0	35.0	29.0	35.0
100-101	31.604536369063588	34.5	32.5	35.0	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	40.0
12	164.0
13	575.0
14	1753.0
15	3786.0
16	6291.0
17	10635.0
18	19498.0
19	34287.0
20	32342.0
21	29221.0
22	28359.0
23	30113.0
24	35346.0
25	43854.0
26	56788.0
27	73855.0
28	95893.0
29	121402.0
30	154228.0
31	199109.0
32	260841.0
33	352791.0
34	511185.0
35	803991.0
36	1414336.0
37	3091890.0
38	4367990.0
39	873993.0
40	499.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.998612200376275	29.38649290102327	24.842222330324788	22.772672568275667
2	24.021257726424	28.161975385283057	26.531217001123764	21.285549887169182
3	23.1121472957706	26.61412236912714	26.85985185428685	23.41387848081541
4	24.271551744233875	26.564964107326457	26.639852622058125	22.52363152638155
5	24.393520411380575	27.472608317791085	25.618364450229535	22.515506820598805
6	24.351913128785295	26.620777230916815	25.061566306902655	23.96574333339523
7	24.44569383538831	26.768994682362106	25.660267774418998	23.125043707830585
8	23.99814145414619	26.52016921301409	25.808603755574357	23.67308557726537
9	23.491458551543236	26.209826824142606	26.192244917149708	24.10646970716445
10-11	23.75149298047302	26.531789075590744	26.06157776477463	23.655140179161606
12-13	23.60759472665603	26.41402511125208	26.297664393738973	23.68071576835292
14-15	23.481130662806287	26.53045364085735	26.308799132046445	23.679616564289923
16-17	23.553792542840252	26.684291613601257	26.29444110495956	23.467474738598934
18-19	23.19712557551113	26.513112744274913	26.686083150172006	23.603678530041947
20-21	23.016024031503616	26.570967095757386	26.705822297888076	23.70718657485092
22-23	23.0018360252089	26.476494965845664	27.158416142798274	23.363252866147164
24-25	23.006063584867864	26.203224719292013	27.58468453910315	23.206027156736972
26-27	23.03525350146641	26.23668170545288	27.6754071791865	23.052657613894212
28-29	22.99814580023556	26.352125692065343	27.645411260559516	23.004317247139582
30-31	23.04990890188626	26.28012861598113	27.626387026762544	23.043575455370064
32-33	23.02466089637698	26.299814580023558	27.64679805816727	23.028726465432193
34-35	23.033590134535174	26.369802422826293	27.621954230937757	22.97465321170078
36-37	23.02640328311493	26.391852899888622	27.536217740657786	23.045526076338664
38-39	23.0652612730644	26.416870570692897	27.483337686007687	23.03453047023502
40-41	23.139319806535006	26.557236950368047	27.210792728014077	23.09265051508287
42-43	23.116091081580432	26.600458844474783	27.14678617837203	23.13666389557276
44-45	23.10617127958402	26.68042807746611	27.127075348564127	23.086325294385734
46-47	23.15271264886078	26.81087890039604	26.94097737745208	23.0954310732911
48-49	23.16494080823829	26.81049193385568	26.855786087061652	23.168781170844376
50-51	23.153644926869145	26.854059504285043	26.863936980123754	23.128358588722055
52-53	23.165596672633978	26.850452250108752	26.85019148474661	23.13375959251066
54-55	23.158049116688385	26.84238944351609	26.849042915447097	23.150518524348428
56-57	23.149275461208155	26.847688201639336	26.858976185041694	23.14406015211081
58-59	23.169072676052064	26.929164794225606	26.82955632965151	23.07220620007082
60-61	23.152159354503002	26.87176784296868	26.820954156264037	23.15511864626428
62-63	23.169816330312273	26.93166880744493	26.816979459986545	23.081535402256254
64-65	23.125577091525876	26.97729089284875	26.824225576261817	23.072906439363557
66-67	23.136343540190065	26.96262876771377	26.786841305707483	23.11418638638868
68-69	23.11739310290876	27.02168976878391	26.767767321254095	23.093149807053234
70-71	23.089024353432233	27.109694672183938	26.7798699239055	23.02141105047833
72-73	23.09236008428037	27.060865100201468	26.75848034880391	23.08829446671425
74-75	23.07385040112901	27.116839524333862	26.76661739616341	23.042692678373715
76-77	23.087932095191235	27.177166480989207	26.713923051578902	23.020978372240652
78-79	23.054474658207084	27.162493037827872	26.71434191456978	23.068690389395268
80-81	23.02970218243298	27.186995462488696	26.753691046934634	23.02961130814369
82-83	23.027999257052343	27.27508458813086	26.72065140436823	22.976264750448568
84-85	23.00091907951709	27.24657889619269	26.75046086334052	23.0020411609497
86-87	22.97642229595476	27.327428759857035	26.744886566618586	22.951262377569616
88-89	23.01601688246769	27.388462856880057	26.701221854849404	22.89429840580285
90-91	22.992165052466685	27.3651375237697	26.672492018095095	22.97020540566852
92-93	22.976029721727436	27.418820623604873	26.664698799117453	22.94045085555024
94-95	22.992363546925514	27.48522147294349	26.628350393774674	22.894064586356322
96-97	22.938920639074823	27.492531831576407	26.634012493358377	22.934535035990393
98-99	22.96721985481251	27.554777590139256	26.589404127887693	22.888598427160538
100-101	22.9764977774041	27.620086891968686	26.493346317490456	22.91006901313676
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27009.0
1	15175.5
2	2384.0
3	1226.5
4	941.0
5	823.0
6	772.0
7	714.0
8	669.5
9	685.0
10	724.5
11	797.5
12	989.0
13	1417.5
14	1894.0
15	2252.0
16	5491.0
17	6050.5
18	3679.0
19	4266.0
20	5672.5
21	7823.0
22	10628.5
23	14541.0
24	19679.5
25	25879.5
26	33471.5
27	42925.0
28	54820.5
29	70028.0
30	88737.5
31	109859.0
32	134452.0
33	167841.5
34	205581.0
35	242902.0
36	283372.5
37	322898.5
38	353035.0
39	377914.5
40	402422.0
41	424248.0
42	448989.5
43	482347.0
44	520795.5
45	551679.0
46	559732.5
47	553194.0
48	547718.0
49	542032.5
50	528259.0
51	508162.0
52	487221.0
53	465727.0
54	437584.0
55	403066.0
56	366069.5
57	325145.5
58	286106.0
59	244957.0
60	202200.5
61	166267.0
62	135726.5
63	106681.5
64	81437.5
65	61664.5
66	45605.0
67	33222.5
68	24467.5
69	18507.5
70	14322.0
71	11120.5
72	8803.5
73	6744.5
74	4927.0
75	3709.5
76	2766.0
77	1911.0
78	1225.5
79	714.0
80	406.5
81	264.5
82	160.5
83	94.5
84	62.5
85	40.0
86	16.5
87	5.0
88	3.0
89	3.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10067123374809513
2	0.0021177308198186418
3	6.479624150191367E-4
4	0.0019280832837154796
5	0.002378496181960489
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.692178738061588E-5
14-15	0.0
16-17	6.005505309933461E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	1.4223565207737145E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	4.1485398522566675E-4
42-43	3.555891301934286E-5
44-45	8.099530187739208E-4
46-47	2.0545149744509208E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	6.321584536772065E-5
56-57	3.555891301934286E-5
58-59	4.741188402579048E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	7.901980670965082E-5
70-71	7.90198067096508E-6
72-73	0.0011931990813157272
74-75	6.835213280384795E-4
76-77	3.5954012052891117E-4
78-79	4.780698305933874E-4
80-81	0.0016633669312381494
82-83	4.6226586925145717E-4
84-85	1.580396134193016E-5
86-87	4.741188402579048E-5
88-89	2.6076536214184764E-4
90-91	1.9359852643864448E-4
92-93	5.570896373030382E-4
94-95	0.002311329346257286
96-97	8.692178738061588E-5
98-99	8.534139124642288E-4
100-101	0.0011497381876254193
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.2655055E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.03156060105946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42237351199238	46.29395094452098
2	12.387371278188542	14.624917165924206
3	4.016708874719818	7.113377800645089
4	1.7774588245858387	4.197046732777075
5	0.9635937749456076	2.844122216025265
6	0.5835792434291975	2.0669756084406665
7	0.3688123279588342	1.5240097101823817
8	0.2587807044245838	1.222098306849974
9	0.17864228151177583	0.9490979400276529
>10	0.8884378640871082	9.631179074991463
>50	0.10434248351194073	4.422401812280075
>100	0.049549220123712934	4.4437168547132915
>500	2.158372755969994E-4	0.07856318521587813
>1k	8.026490905750713E-5	0.11581451245947101
>5k	2.6754167969246857E-5	0.11329397554587244
>10k+	2.6754167969246857E-5	0.3594341594006744
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31828	0.25150424079547656	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13689	0.10817021340484098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.482376805158096E-5	7.90198067096508E-6	0.0	0.0	7.90198067096508E-6
2	9.482376805158096E-5	7.90198067096508E-6	0.0	7.90198067096508E-6	7.90198067096508E-6
3	1.1062772939351114E-4	7.90198067096508E-6	0.0	1.580396134193016E-5	7.90198067096508E-6
4	1.1062772939351114E-4	7.90198067096508E-6	0.0	1.580396134193016E-5	7.90198067096508E-6
5	1.1062772939351114E-4	7.90198067096508E-6	0.0	1.580396134193016E-5	7.90198067096508E-6
6	1.1852971006447621E-4	7.90198067096508E-6	0.0	1.580396134193016E-5	7.90198067096508E-6
7	1.1852971006447621E-4	1.580396134193016E-5	0.0	1.580396134193016E-5	7.90198067096508E-6
8	1.264316907354413E-4	1.580396134193016E-5	0.0	1.580396134193016E-5	7.90198067096508E-6
9	1.264316907354413E-4	1.580396134193016E-5	0.0	2.370594201289524E-5	1.580396134193016E-5
10-11	1.264316907354413E-4	2.370594201289524E-5	0.0	2.370594201289524E-5	1.97549516774127E-5
12-13	1.3433367140640637E-4	2.370594201289524E-5	0.0	3.95099033548254E-5	3.95099033548254E-5
14-15	1.9359852643864448E-4	2.370594201289524E-5	0.0	4.741188402579049E-5	3.95099033548254E-5
16-17	3.2793219784505085E-4	2.7656932348377784E-5	0.0	5.531386469675557E-5	3.95099033548254E-5
18-19	3.5558913019342864E-4	3.160792268386032E-5	0.0	5.531386469675557E-5	4.741188402579048E-5
20-21	3.990500238837366E-4	4.346089369030794E-5	0.0	5.531386469675557E-5	5.926485503223811E-5
22-23	4.938737919353176E-4	4.741188402579048E-5	0.0	5.531386469675557E-5	7.111782603868573E-5
24-25	5.689426083094858E-4	4.741188402579048E-5	0.0	6.321584536772065E-5	7.111782603868573E-5
26-27	6.914233087094445E-4	4.741188402579048E-5	0.0	6.716683570320319E-5	7.111782603868573E-5
28-29	8.178549994448858E-4	4.741188402579048E-5	0.0	7.111782603868573E-5	7.90198067096508E-5
30-31	9.561396611867748E-4	4.741188402579048E-5	0.0	7.111782603868573E-5	7.90198067096508E-5
32-33	0.001090473332593181	5.531386469675557E-5	0.0	8.692178738061588E-5	9.87747583870635E-5
34-35	0.0012248070039995876	5.531386469675557E-5	0.0	9.482376805158096E-5	1.1062772939351114E-4
36-37	0.0014302585014446796	5.531386469675557E-5	0.0	9.482376805158096E-5	1.2248070039995874E-4
38-39	0.0016041020762059114	5.531386469675557E-5	0.0	9.482376805158096E-5	1.382846617418889E-4
40-41	0.0018530144673413115	5.531386469675557E-5	0.0	9.87747583870635E-5	1.5013763274833653E-4
42-43	0.002026858042102543	5.531386469675557E-5	0.0	1.0272574872254606E-4	1.580396134193016E-4
44-45	0.0022520644912250483	5.531386469675557E-5	0.0	1.1457871972899367E-4	1.7384357476123177E-4
46-47	0.002532584805044308	6.321584536772065E-5	0.0	1.2248070039995874E-4	1.8569654576767937E-4
48-49	0.002990899683960283	7.111782603868573E-5	0.0	1.264316907354413E-4	1.8964753610316193E-4
50-51	0.0034255086208633623	7.111782603868573E-5	0.0	1.3433367140640637E-4	1.9359852643864448E-4
52-53	0.003824558644747099	7.90198067096508E-5	0.0	1.4223565207737145E-4	1.97549516774127E-4
54-55	0.004496227001779131	7.90198067096508E-5	0.0	1.5013763274833653E-4	1.97549516774127E-4
56-57	0.005029610697069274	7.90198067096508E-5	0.0	1.659415940902667E-4	2.0940248778057464E-4
58-59	0.005685475092759375	7.90198067096508E-5	0.0	1.7384357476123177E-4	2.1730446845153974E-4
60-61	0.006669271686294528	7.90198067096508E-5	0.0	1.7779456509671432E-4	2.2125545878702227E-4
62-63	0.007799254922242534	8.692178738061588E-5	0.0	1.8174555543219685E-4	2.2125545878702227E-4
64-65	0.00928482728838397	8.692178738061588E-5	0.0	1.97549516774127E-4	2.4101041046443495E-4
66-67	0.011355146224176821	9.482376805158096E-5	0.0	2.0940248778057464E-4	2.449614007999175E-4
68-69	0.01398255479727271	9.482376805158096E-5	0.0	2.1730446845153974E-4	2.528633814708826E-4
70-71	0.018763253103206583	9.482376805158096E-5	0.0	2.2915743945798735E-4	2.6866734281281275E-4
72-73	0.024440826215294996	9.482376805158096E-5	0.0	2.449614007999175E-4	2.6866734281281275E-4
74-75	0.030047281501344718	1.1852971006447621E-4	0.0	2.449614007999175E-4	2.6866734281281275E-4
76-77	0.036613827438916696	1.1852971006447621E-4	0.0	2.489123911354E-4	2.6866734281281275E-4
78-79	0.045815683930255535	1.2248070039995874E-4	0.0	2.528633814708826E-4	2.7656932348377785E-4
80-81	0.05753037027496127	1.3433367140640637E-4	0.0	2.528633814708826E-4	2.7656932348377785E-4
82-83	0.07335013557823336	1.3433367140640637E-4	0.0	2.568143718063651E-4	2.805203138192604E-4
84-85	0.09389528532274258	1.3433367140640637E-4	0.0	2.6076536214184764E-4	2.884222944902255E-4
86-87	0.12012195916967566	1.3433367140640637E-4	0.0	2.6866734281281275E-4	2.92373284825708E-4
88-89	0.1535828963208773	1.382846617418889E-4	0.0	2.6866734281281275E-4	2.9632427516119054E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	5915	0.0	82.88156	1
TCGACGG	6495	0.0	75.69469	2
CGACGGC	8355	0.0	58.72881	3
ACGGCGC	9245	0.0	53.022243	5
GACGGCG	9640	0.0	50.80078	4
TATCTCG	3900	0.0	48.840935	2
CGGCGCG	10015	0.0	48.802975	6
ATCTCGA	8545	0.0	40.288445	1
TCTCGAG	9820	0.0	34.827732	2
CGCGCCG	22610	0.0	30.272293	9
GGCGCGC	23390	0.0	29.404936	7
GCGCGCC	23570	0.0	29.119919	8
TCGAGGG	12175	0.0	28.44116	4
CTCGAGG	12540	0.0	27.500235	3
CGAGGGC	13250	0.0	26.384401	5
GGGCGCG	13230	0.0	26.13686	8
AGGGCGC	13705	0.0	25.230988	7
ATATCTC	7935	0.0	24.329496	1
GAGGGCG	15445	0.0	22.57304	6
GCCGGAT	23095	0.0	17.410028	12-13
>>END_MODULE