FastQCFastQC Report
Tue 31 May 2016
SRR1051282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13190722
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT3329922.52444104272685No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT551610.4181802936943103No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA238540.1808392292703917No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA177120.13427619807316082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA144490.10953911393174688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1268700.094.306841
TCGACGG1291100.092.696412
CGACGGC1304550.091.6278153
ACGGCGC1318450.090.605365
GACGGCG1320250.090.5321964
CGGCGCG1322350.090.311456
TATCTCG660650.089.578172
ATATCTC682750.086.866481
ATCTCGA707550.083.492833
TCTCGAG701300.082.06044
CTCGAGG723000.079.6105965
TCGAGGG726850.079.138676
CGAGGGC736450.077.976877
AGGGCGC746450.077.1283349
GAGGGCG761850.075.637858
CGCGCCG2057150.058.5082179
GGCGCGC2065700.058.3612067
GCGCGCC2064950.058.3263178
CTCGACG25450.057.114675
TCCATAT1427200.041.65402218-19
Produced by FastQC (version 0.11.5)