##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051282_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13190722 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.110264169012126 33.0 31.0 34.0 31.0 34.0 2 32.36326783325431 34.0 31.0 34.0 31.0 34.0 3 32.51242160967383 34.0 31.0 34.0 31.0 34.0 4 36.0511882518637 37.0 37.0 37.0 35.0 37.0 5 35.99681829394934 37.0 35.0 37.0 35.0 37.0 6 36.01626408319423 37.0 36.0 37.0 35.0 37.0 7 36.00765735188718 37.0 36.0 37.0 35.0 37.0 8 36.010386315472346 37.0 36.0 37.0 35.0 37.0 9 37.70503070264084 39.0 38.0 39.0 35.0 39.0 10-11 37.70880570449442 39.0 38.0 39.0 35.0 39.0 12-13 37.68039114917288 39.0 38.0 39.0 35.0 39.0 14-15 39.110520296008055 41.0 39.0 41.0 36.0 41.0 16-17 39.078774308184194 40.5 39.0 41.0 36.0 41.0 18-19 39.0384382295374 40.5 39.0 41.0 36.0 41.0 20-21 39.09066467324533 41.0 39.0 41.0 36.0 41.0 22-23 38.97000061861663 40.5 39.0 41.0 35.5 41.0 24-25 38.91505313355857 40.5 39.0 41.0 35.0 41.0 26-27 38.91869830931165 40.0 39.0 41.0 35.0 41.0 28-29 38.71035326951777 40.0 38.0 41.0 35.0 41.0 30-31 38.27368763438423 40.0 37.0 41.0 35.0 41.0 32-33 38.06838427797963 40.0 36.0 41.0 34.5 41.0 34-35 37.87168606085399 40.0 35.0 41.0 34.0 41.0 36-37 37.780263847574076 40.0 35.0 41.0 34.0 41.0 38-39 37.53446085058877 40.0 35.0 41.0 33.0 41.0 40-41 36.53022120396442 40.0 35.0 41.0 29.0 41.0 42-43 36.48706924457964 40.0 35.0 41.0 29.0 41.0 44-45 36.44245182333461 40.0 35.0 41.0 29.0 41.0 46-47 36.62008061423779 40.0 35.0 41.0 29.0 41.0 48-49 36.63387443841209 40.0 35.0 41.0 29.0 41.0 50-51 36.38000584804986 39.5 35.0 40.5 28.5 41.0 52-53 36.26115833538149 39.0 35.0 40.5 29.0 41.0 54-55 36.150989801771274 39.0 35.0 41.0 28.5 41.0 56-57 35.869863264497575 39.0 35.0 41.0 27.5 41.0 58-59 35.564857935752116 39.0 35.0 41.0 27.0 41.0 60-61 35.2325100172682 38.5 34.5 41.0 26.0 41.0 62-63 34.99948812506245 38.0 34.0 40.5 26.0 41.0 64-65 34.71779421930051 37.5 34.0 40.0 25.5 41.0 66-67 34.35747357119648 37.0 34.0 40.0 25.0 41.0 68-69 34.03284179592292 36.5 34.0 39.0 25.0 41.0 70-71 33.64459197153878 36.0 34.0 39.0 25.0 41.0 72-73 33.24167767314026 35.5 34.0 39.0 24.5 40.5 74-75 32.85650508743949 35.0 34.0 37.0 24.0 39.5 76-77 32.48435241073233 35.0 33.0 37.0 23.5 39.0 78-79 32.15194073531381 35.0 33.0 36.5 23.0 39.0 80-81 31.81814001538354 35.0 33.0 36.0 21.5 37.0 82-83 31.551031323380176 35.0 33.0 36.0 20.0 37.0 84-85 31.31175548995726 35.0 33.0 35.0 19.5 37.0 86-87 31.09826247570072 35.0 33.0 35.0 19.0 36.0 88-89 30.903785630536373 35.0 33.0 35.0 18.0 36.0 90-91 30.713569545321324 35.0 32.5 35.0 17.0 36.0 92-93 30.505576192114425 35.0 32.0 35.0 9.5 35.5 94-95 30.324700801063052 35.0 32.0 35.0 4.5 35.0 96-97 30.105482815876186 35.0 32.0 35.0 2.0 35.0 98-99 29.851069297040752 35.0 32.0 35.0 2.0 35.0 100-101 28.773562546462582 34.5 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35068572964706846 1101 2 0.3591000458890008 1101 3 0.3609480063055841 1101 4 0.2756890644180672 1101 5 0.28789805767104326 1101 6 0.285658128093111 1101 7 0.29682454232334265 1101 8 0.2510067017083415 1101 9 0.35914718695590153 1101 10-11 0.3532797567425021 1101 12-13 0.362055798118746 1101 14-15 0.48422952837906763 1101 16-17 0.42222823792960185 1101 18-19 0.48804210626705924 1101 20-21 0.4962618180092946 1101 22-23 0.5645045182207724 1101 24-25 0.5471587410027468 1101 26-27 0.5028951589902988 1101 28-29 0.5083990059204808 1101 30-31 0.5043201629739542 1101 32-33 0.4816595448880747 1101 34-35 0.5040498615430167 1101 36-37 0.4786606388318049 1101 38-39 0.46059256500658563 1101 40-41 0.3499525121977456 1101 42-43 0.36546260659226704 1101 44-45 0.3296942186035068 1101 46-47 0.4099501937280863 1101 48-49 0.4021316424085626 1101 50-51 0.35265723718542574 1101 52-53 0.2987477413998576 1101 54-55 0.28924941910273105 1101 56-57 0.3048317511148184 1101 58-59 0.27270357187776284 1101 60-61 0.24938252214857215 1101 62-63 0.29554187336795934 1101 64-65 0.2817622145555063 1101 66-67 0.3154977825499259 1101 68-69 0.2432125521279076 1101 70-71 0.19800200599316042 1101 72-73 0.20349030928905876 1101 74-75 0.16890774715525225 1101 76-77 0.14404549847331793 1101 78-79 0.1246431007845814 1101 80-81 0.1329530935694727 1101 82-83 0.1296485233941702 1101 84-85 0.15113732330825158 1101 86-87 0.11339866753270655 1101 88-89 0.07491912329929917 1101 90-91 0.05303028231261564 1101 92-93 0.046024104569994506 1101 94-95 0.1531604937210176 1101 96-97 0.1675868161341576 1101 98-99 0.1340629057258873 1101 100-101 0.2884799226309589 1102 1 0.25264325395666276 1102 2 0.26496456999191054 1102 3 0.2577219679804017 1102 4 0.13671194161712918 1102 5 0.233961077209365 1102 6 0.23836503950332855 1102 7 0.20645678219774055 1102 8 0.2190908378288441 1102 9 0.2547871687323138 1102 10-11 0.2750868516892595 1102 12-13 0.2666946013786884 1102 14-15 0.3858269938393377 1102 16-17 0.371534872653136 1102 18-19 0.4136516685576126 1102 20-21 0.3949215975038882 1102 22-23 0.39750209882220133 1102 24-25 0.39742244290157913 1102 26-27 0.37803286016217186 1102 28-29 0.43253831172737733 1102 30-31 0.406782897537866 1102 32-33 0.38471551738874155 1102 34-35 0.39830864416097 1102 36-37 0.38725616279225505 1102 38-39 0.35384846315258756 1102 40-41 0.24767167689878988 1102 42-43 0.21064310441406775 1102 44-45 0.2067209100690306 1102 46-47 0.20808652248550885 1102 48-49 0.21986448677176895 1102 50-51 0.19794936657265083 1102 52-53 0.16217612150841632 1102 54-55 0.2637052533530806 1102 56-57 0.26147783413878045 1102 58-59 0.27924253389416265 1102 60-61 0.1955398759312601 1102 62-63 0.2308824020165403 1102 64-65 0.2639638108504201 1102 66-67 0.2771592329018375 1102 68-69 0.15281059476617287 1102 70-71 0.16751373390424362 1102 72-73 0.1330359791029352 1102 74-75 0.17109517579152822 1102 76-77 0.14543582216337825 1102 78-79 0.08505997383139885 1102 80-81 0.13768598653362574 1102 82-83 0.16655003947269265 1102 84-85 0.17315028742288874 1102 86-87 0.12038312842602394 1102 88-89 0.13510710788847646 1102 90-91 0.16822555198562839 1102 92-93 0.19043461837149067 1102 94-95 0.16680167750600106 1102 96-97 0.19759651207234086 1102 98-99 0.21718026471531715 1102 100-101 0.4044784660748171 1103 1 0.25954719331599563 1103 2 0.22541218898241766 1103 3 0.25064875130910735 1103 4 0.21472501898077212 1103 5 0.23937102036084212 1103 6 0.26124252923175817 1103 7 0.2310131413909815 1103 8 0.2200335736508734 1103 9 0.3177063695930684 1103 10-11 0.27629931253520823 1103 12-13 0.2680601483505711 1103 14-15 0.37967905888522324 1103 16-17 0.4037276609655578 1103 18-19 0.42965404559966913 1103 20-21 0.3607295772224788 1103 22-23 0.4360699648077997 1103 24-25 0.46038364027441503 1103 26-27 0.41422526615138366 1103 28-29 0.38933978438435446 1103 30-31 0.4020063128932705 1103 32-33 0.3939911321872671 1103 34-35 0.3722316498864089 1103 36-37 0.37876751018179533 1103 38-39 0.40999023069571194 1103 40-41 0.3287125679884326 1103 42-43 0.3234057885868893 1103 44-45 0.36662642593736905 1103 46-47 0.3337308199898885 1103 48-49 0.3406963413458044 1103 50-51 0.23594798476520396 1103 52-53 0.20881946724023237 1103 54-55 0.25176714745229134 1103 56-57 0.2992514690669523 1103 58-59 0.26832496822247265 1103 60-61 0.3236492282307424 1103 62-63 0.2729826928536099 1103 64-65 0.26665646818041466 1103 66-67 0.3180772312203288 1103 68-69 0.27073108134756296 1103 70-71 0.21682203253764953 1103 72-73 0.20059683700824138 1103 74-75 0.20174544682839013 1103 76-77 0.24659037288040508 1103 78-79 0.21141655544448668 1103 80-81 0.2016852485775189 1103 82-83 0.16077428658663706 1103 84-85 0.1450641709398397 1103 86-87 0.14803712091403654 1103 88-89 0.1271300969664786 1103 90-91 0.17045844493046047 1103 92-93 0.1763272924698569 1103 94-95 0.198242336253859 1103 96-97 0.1994757594058214 1103 98-99 0.24672890101831513 1103 100-101 0.41113462664273825 1104 1 0.17667426020860688 1104 2 0.18690083051098583 1104 3 0.1579981311179779 1104 4 0.13696433664885177 1104 5 0.16566872208999683 1104 6 0.18879064823451586 1104 7 0.1661763330611592 1104 8 0.1676859395995578 1104 9 0.24015611778213497 1104 10-11 0.23594985968011173 1104 12-13 0.2252503253319773 1104 14-15 0.30673034956048895 1104 16-17 0.30785421134509505 1104 18-19 0.3411493715652938 1104 20-21 0.33713684140593614 1104 22-23 0.3996020488672585 1104 24-25 0.4134695021386676 1104 26-27 0.3010719914298434 1104 28-29 0.36108683026811406 1104 30-31 0.34687201261613154 1104 32-33 0.3111299776850345 1104 34-35 0.3544762459107176 1104 36-37 0.3318226714026338 1104 38-39 0.30131471695725764 1104 40-41 0.18399562328155383 1104 42-43 0.12239944811184245 1104 44-45 0.13450125439374006 1104 46-47 0.16328888758940963 1104 48-49 0.15719501808085568 1104 50-51 0.09155057403757638 1104 52-53 0.08072078297254848 1104 54-55 0.09295046441837229 1104 56-57 0.15106446904584203 1104 58-59 0.07339543070060017 1104 60-61 0.07601512678636624 1104 62-63 0.1254770405188026 1104 64-65 0.13775139960994665 1104 66-67 0.1232478314994907 1104 68-69 0.0871505896190996 1104 70-71 0.0800634590714111 1104 72-73 0.07914207725070099 1104 74-75 0.14729571507275097 1104 76-77 0.10648990621410803 1104 78-79 0.08534426795090155 1104 80-81 0.06852347525425984 1104 82-83 0.027889506073826453 1104 84-85 0.05146458772790652 1104 86-87 0.056341793367973736 1104 88-89 0.06096977403140613 1104 90-91 0.048994243811012694 1104 92-93 0.07984868457517891 1104 94-95 0.1199423650202327 1104 96-97 0.17120767021441807 1104 98-99 0.1804848167107096 1104 100-101 0.3865670254889544 1105 1 0.0842783138464327 1105 2 0.04723970193480653 1105 3 0.10713368592255534 1105 4 0.07088268100427086 1105 5 0.04826516451278451 1105 6 0.09800301529656252 1105 7 0.09669176507799904 1105 8 0.07438102087521514 1105 9 0.10018991709937808 1105 10-11 0.06420646210854386 1105 12-13 0.11315463494533873 1105 14-15 0.18719685424647992 1105 16-17 0.21118603074882714 1105 18-19 0.21708942119985863 1105 20-21 0.15319980526027877 1105 22-23 0.23281764960455575 1105 24-25 0.20668043638613653 1105 26-27 0.1785155700138361 1105 28-29 0.22766975723946103 1105 30-31 0.2322501158674939 1105 32-33 0.171738586294353 1105 34-35 0.18068951099815678 1105 36-37 0.212671691974208 1105 38-39 0.18625564074588397 1105 40-41 0.07647586307090393 1105 42-43 0.08417999233197548 1105 44-45 0.033140476922149276 1105 46-47 0.017215374920589 1105 48-49 0.06183127682261613 1105 50-51 0.014989522295032032 1105 52-53 0.009323559850201946 1105 54-55 0.04103658899076379 1105 56-57 0.053820080724761965 1105 58-59 0.05588162407231323 1105 60-61 0.024712958788100536 1105 62-63 0.05233454663761705 1105 64-65 0.07359400520542181 1105 66-67 0.12455097929137793 1105 68-69 0.0477992762413777 1105 70-71 0.04809072654990132 1105 72-73 0.0619748681643415 1105 74-75 0.06818783676597917 1105 76-77 0.06665177791921906 1105 78-79 0.03267418334839789 1105 80-81 -0.001185184892207758 1105 82-83 0.0012248322480168383 1105 84-85 0.007674648890187541 1105 86-87 -0.0290250912463641 1105 88-89 -0.00808353333323808 1105 90-91 -0.06485124219958038 1105 92-93 -0.035044027131945654 1105 94-95 -0.028858692693709997 1105 96-97 0.03917431964265461 1105 98-99 0.04627401286485977 1105 100-101 0.25623989199278796 1106 1 0.09996676248513126 1106 2 0.033125458885393755 1106 3 0.007772511670708582 1106 4 0.05174834081014268 1106 5 0.03184885047904373 1106 6 0.05253751789315686 1106 7 0.02522324937292808 1106 8 0.04964334540150617 1106 9 0.04533950368171702 1106 10-11 0.0777316830396586 1106 12-13 0.04428103755419954 1106 14-15 0.12201598433944127 1106 16-17 0.10479648721410939 1106 18-19 0.14551158120803365 1106 20-21 0.043101037760727934 1106 22-23 0.1283000248278796 1106 24-25 0.11783327585276027 1106 26-27 0.10066560965316995 1106 28-29 0.08529117406845188 1106 30-31 0.0888810910587381 1106 32-33 0.08633810522744767 1106 34-35 0.10956419241816917 1106 36-37 0.12917549099560688 1106 38-39 0.11778753103477158 1106 40-41 0.10326812162013965 1106 42-43 0.014324039972855473 1106 44-45 0.024272549397565513 1106 46-47 0.005901447527229209 1106 48-49 0.012556518425000718 1106 50-51 -0.1457131835046681 1106 52-53 -0.050516584392219954 1106 54-55 -0.01188577351788922 1106 56-57 0.02603990950436952 1106 58-59 0.03618736237561393 1106 60-61 0.017799300564036002 1106 62-63 -0.0019512406019615014 1106 64-65 0.007243967270518681 1106 66-67 0.10071249754128786 1106 68-69 0.05287298226819814 1106 70-71 0.06432582836669098 1106 72-73 0.06075741616564301 1106 74-75 0.041774991575771026 1106 76-77 -0.01358911318799727 1106 78-79 -0.01982542499550277 1106 80-81 0.00999379319805982 1106 82-83 0.03162499819119802 1106 84-85 -0.022108948164220266 1106 86-87 -0.022511628349359825 1106 88-89 0.03174583036501488 1106 90-91 -0.014396083971782048 1106 92-93 0.05030222318917055 1106 94-95 0.019172134033279775 1106 96-97 0.0174289195394266 1106 98-99 -0.026436205894654563 1106 100-101 0.219789951250128 1107 1 0.005319102375722196 1107 2 -0.01771163137642162 1107 3 6.812233272910362E-4 1107 4 -0.011845706026377911 1107 5 -0.07438505770647197 1107 6 -0.08056745910180041 1107 7 -0.04714287356233626 1107 8 -0.060580189655979666 1107 9 -0.06762085664127682 1107 10-11 -0.017281481376762997 1107 12-13 -0.06388254118343184 1107 14-15 -0.06779501218688466 1107 16-17 -0.02732056752254408 1107 18-19 -0.004809354319249337 1107 20-21 -0.015206482139596744 1107 22-23 -0.03603927465857737 1107 24-25 0.018251481062627306 1107 26-27 -0.046524871670669654 1107 28-29 -0.07170160731768505 1107 30-31 -0.04698871084776357 1107 32-33 -0.044482661492210696 1107 34-35 -0.02685956223004382 1107 36-37 -0.10750433527745429 1107 38-39 -0.2651751718687194 1107 40-41 -0.28218372815196346 1107 42-43 -0.2113320134205523 1107 44-45 -0.12838662017828995 1107 46-47 -0.2699608716170374 1107 48-49 -0.20181529281364163 1107 50-51 -0.19268071420039234 1107 52-53 -0.11309244823405606 1107 54-55 -0.05519342006199679 1107 56-57 -0.05426819592215537 1107 58-59 -0.08785434644372003 1107 60-61 -0.1434450283089177 1107 62-63 -0.18501203407366518 1107 64-65 -0.08503038272687746 1107 66-67 -0.020586997269930407 1107 68-69 -0.04224062303841691 1107 70-71 -0.050890428229614315 1107 72-73 -0.07389122273455229 1107 74-75 -0.039574498586539164 1107 76-77 -0.019914995559787485 1107 78-79 -0.08894208159695438 1107 80-81 -0.0915340103209239 1107 82-83 -0.04271186987701725 1107 84-85 -0.07932079216356414 1107 86-87 -0.06911011501439646 1107 88-89 -0.051131194106392996 1107 90-91 -0.12662348212900199 1107 92-93 -0.03877626611639684 1107 94-95 -0.0253191777766979 1107 96-97 -0.041067485106498935 1107 98-99 -0.06697250483075479 1107 100-101 0.14575353701811622 1108 1 -0.257609771765523 1108 2 -0.17263780621195224 1108 3 -0.20204126993113647 1108 4 -0.1307498238050826 1108 5 -0.11869541818582974 1108 6 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-0.6731213735806989 2308 38-39 -0.7930423185195679 2308 40-41 -0.8948911934551944 2308 42-43 -0.7877787844542468 2308 44-45 -0.7671383068537523 2308 46-47 -0.9001425245002679 2308 48-49 -0.7558152183316764 2308 50-51 -1.2608987008553356 2308 52-53 -1.1971323455687894 2308 54-55 -0.832184100496093 2308 56-57 -0.8969179664501894 2308 58-59 -1.1213591243324643 2308 60-61 -1.1373498809690261 2308 62-63 -1.0785666887050596 2308 64-65 -1.259608933795441 2308 66-67 -1.0105083711160745 2308 68-69 -0.935523165279939 2308 70-71 -0.8550254661110799 2308 72-73 -0.8847421795952997 2308 74-75 -1.1374724900395208 2308 76-77 -0.9916161822579035 2308 78-79 -0.9680735316440554 2308 80-81 -1.0499028480058605 2308 82-83 -0.931840121045461 2308 84-85 -0.928413326514125 2308 86-87 -0.9229023827907596 2308 88-89 -0.9764290000177631 2308 90-91 -1.0931791410189682 2308 92-93 -1.0088533427599131 2308 94-95 -1.0033923269740335 2308 96-97 -1.0563740140118547 2308 98-99 -1.0794402580534097 2308 100-101 -0.5183664723465391 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 125.0 11 967.0 12 3118.0 13 6281.0 14 9467.0 15 13724.0 16 18600.0 17 29737.0 18 67797.0 19 198528.0 20 208977.0 21 138941.0 22 115820.0 23 107575.0 24 111163.0 25 124362.0 26 146693.0 27 172325.0 28 196313.0 29 217500.0 30 240197.0 31 274837.0 32 331744.0 33 416855.0 34 558682.0 35 819352.0 36 1409985.0 37 2920448.0 38 3665752.0 39 664370.0 40 486.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.13173438241302 32.78445776280199 19.197592627775087 17.8862152270099 2 20.85554190947681 26.066326311660028 35.99370201199881 17.08442976686435 3 20.71090429697658 27.962136617317245 23.077047295415262 28.24991179029091 4 30.595377837903857 22.93878701546566 27.224324436407077 19.2415107102234 5 21.101648962351327 33.32986343511735 21.924803337895504 23.64368426463582 6 21.059903187533404 23.021580006747307 25.305793206448605 30.612723599270687 7 30.552281765029733 22.840454377359915 22.138546612889066 24.46871724472129 8 34.275265599563085 23.039042138860935 22.01884779316856 20.666844468407415 9 20.555432826194046 26.945712296870482 22.319407535084128 30.17944734185134 10-11 29.91835852502994 22.79287289960322 22.268686278127912 25.020082297238922 12-13 27.398094660777478 22.586728004729384 22.640182243246425 27.374995091246713 14-15 27.18376977393656 22.65653085555135 22.688337302537345 27.47136206797475 16-17 27.193591071057373 27.41689196391221 22.55399287468874 22.83552409034168 18-19 22.56570565280657 22.711652933023682 27.483408413883637 27.239233000286113 20-21 22.182072368745242 29.810741216439858 23.030267031630263 24.976919383184633 22-23 19.840854806886234 27.3065606264767 30.20446113563761 22.648123430999455 24-25 19.81914257612282 22.67710213284762 35.15474361448903 22.349011676540524 26-27 19.759388455006484 22.696289861919613 37.66551595886867 19.87880572420524 28-29 19.75620060827603 22.56180518397704 37.86953056853143 19.8124636392155 30-31 19.799348360157996 22.492650516021794 37.85262095585063 19.855380167969578 32-33 19.782868176213146 22.51465157082283 37.869826814075616 19.8326534388884 34-35 19.772907780132254 22.631974500558936 37.80702502187049 19.788092697438326 36-37 19.811447773670007 22.61256813690714 37.67697098005704 19.89901310936581 38-39 20.04533728470656 22.759517063874263 36.87931831609851 20.31582733532067 40-41 21.446475788057697 24.046314523192894 32.50683321201068 22.000376476738726 42-43 21.535945020631402 24.407837237670634 32.80900280186178 21.24721493983618 44-45 21.822648893021107 25.242729634282334 31.78445252920941 21.150168943487145 46-47 21.448833505853585 26.05885409456738 31.44232362716764 21.049988772411396 48-49 21.431855663397346 26.644072250177057 30.630533340024908 21.29353874640069 50-51 21.53800492278931 26.873581504534354 30.11110675197682 21.47730682069952 52-53 21.61465075423724 26.84481029726371 29.962323200949527 21.578215747549525 54-55 21.768953533858753 26.544419999575457 29.92942365367062 21.757202812895166 56-57 21.78113037501719 26.34998122162111 30.070825646732068 21.798062756629633 58-59 21.799042226466206 26.326073835449098 30.097970944900442 21.77691299318425 60-61 21.855874507791768 26.335338537334096 29.93413367882628 21.874653276047855 62-63 21.984772364083423 26.451133384271042 29.628438769288717 21.935655482356818 64-65 22.078765406884425 26.58126275101847 29.299203153030113 22.04076868906699 66-67 22.169225900745822 26.614456209068464 29.006125391527256 22.210192498658454 68-69 22.197719730580328 26.795011675630796 28.765699860856746 22.241568732932134 70-71 22.261863300583546 26.91319701832849 28.59115293309949 22.233786747988475 72-73 22.307762986741743 26.90543019555715 28.421510968088025 22.365295849613084 74-75 22.38303837259836 26.951466755012426 28.344083730854496 22.321411141534718 76-77 22.43789508541828 27.019979982566245 28.199434714463482 22.342690217551993 78-79 22.426688067631538 26.923047617791262 28.14939538369785 22.50086893087935 80-81 22.432015182089206 26.981573817294773 28.077839442295367 22.508571558320657 82-83 22.487938416146942 27.07565966521981 27.99009941635804 22.446302502275213 84-85 22.482299083428952 26.998830723065133 28.00545811665333 22.513412076852582 86-87 22.5187102616135 27.007717454599295 27.977628426057592 22.49594385772961 88-89 22.567131113586974 27.05934882008628 27.845826558232712 22.527693508094035 90-91 22.6412444492762 26.991811802265865 27.702663797785824 22.66427995067211 92-93 22.58787532274277 27.035003733245834 27.707181307850192 22.669939636161203 94-95 22.61315092821187 27.104993540340867 27.648599546734076 22.633255984713184 96-97 22.654938827457663 27.026568371314326 27.661836857755016 22.656655943472995 98-99 22.726830264196014 27.053399979667375 27.621398170503397 22.598371585633217 100-101 22.859568270930687 27.07436993392304 27.44326297052161 22.62279882462466 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 13357.0 1 7712.0 2 1570.5 3 883.5 4 609.5 5 480.5 6 444.5 7 436.0 8 424.0 9 412.0 10 421.5 11 476.0 12 728.5 13 1389.5 14 2092.0 15 4048.5 16 70786.0 17 81099.0 18 24673.5 19 21356.0 20 19294.0 21 19823.5 22 22783.0 23 27291.0 24 33211.5 25 41006.5 26 50841.0 27 63441.5 28 78692.0 29 97885.0 30 121846.5 31 149338.5 32 180557.5 33 218018.5 34 257725.0 35 295616.0 36 334375.0 37 374483.0 38 407600.0 39 431962.5 40 450808.5 41 461970.0 42 476459.5 43 500659.0 44 530544.0 45 556077.0 46 559164.0 47 546555.5 48 533468.0 49 520591.5 50 501882.0 51 477683.5 52 454458.0 53 433659.5 54 407461.5 55 374757.5 56 338363.5 57 298622.5 58 260448.0 59 220815.0 60 182402.5 61 150382.0 62 122276.0 63 95758.0 64 73148.0 65 54624.0 66 39963.0 67 29180.0 68 21736.0 69 16629.5 70 12410.0 71 9410.5 72 7371.5 73 5444.0 74 4025.5 75 3077.0 76 2183.5 77 1465.5 78 972.5 79 586.0 80 317.0 81 181.5 82 96.5 83 32.5 84 14.0 85 11.0 86 6.5 87 6.0 88 6.0 89 3.5 90 3.0 91 3.0 92 3.0 93 3.0 94 2.5 95 1.0 96 0.0 97 0.5 98 2.0 99 4.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001819460678498114 2 0.013251738608394598 3 0.004275732594470568 4 0.005011097952030222 5 0.008293708259487238 6 0.0020620554356311958 7 7.581086160408808E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 5.420476604692298E-4 34-35 1.1371629240613213E-5 36-37 0.0 38-39 2.539663863736951E-4 40-41 0.0 42-43 2.1606095557165104E-4 44-45 5.193044019880034E-4 46-47 0.0 48-49 0.0 50-51 2.2364204173205986E-4 52-53 8.415005638053776E-4 54-55 3.1840561873716996E-4 56-57 1.5162172320817618E-4 58-59 1.9331769709042464E-4 60-61 0.0022250487880799853 62-63 0.005731301137269059 64-65 8.263383914845602E-4 66-67 0.0053674090015694365 68-69 0.0 70-71 0.0 72-73 0.0 74-75 9.438452269708966E-4 76-77 0.001667838955289938 78-79 0.005022469581270835 80-81 0.0033546306259808977 82-83 0.0014138725689162427 84-85 6.93669383677406E-4 86-87 0.0017663930753752523 88-89 0.002016568918668743 90-91 0.0016109808090868716 92-93 0.00346834691838703 94-95 7.960140468429249E-5 96-97 0.0 98-99 7.50527529880472E-4 100-101 3.1840561873716996E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3190722E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.50692959743893 #Duplication Level Percentage of deduplicated Percentage of total 1 77.59729590746464 43.07187640887277 2 12.678470800380856 14.074859722398509 3 4.23701247268657 7.055506590746523 4 1.940428824434241 4.308289845868504 5 1.0061290858810106 2.7923568167966417 6 0.5850931466988718 1.9486034461054975 7 0.39605379891976283 1.538861123340626 8 0.266761554348074 1.184569185320154 9 0.20128945712132554 1.005566375262611 >10 0.9391045289135808 9.341144924580039 >50 0.09379581341986987 3.719812179988681 >100 0.056808891925475696 4.929057496949189 >500 7.994325241000775E-4 0.29982229852629666 >1k 7.677266379117409E-4 0.8150787630574671 >5k 8.081084727681734E-5 0.33027193083338674 >10k+ 1.077477963690898E-4 3.584322891353289 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 332992 2.52444104272685 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 55161 0.4181802936943103 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 23854 0.1808392292703917 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 17712 0.13427619807316082 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14449 0.10953911393174688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2129737856654094E-4 0.0 0.0 7.5810861604088085E-6 0.0 2 1.2129737856654094E-4 0.0 0.0 1.5162172320817617E-5 7.5810861604088085E-6 3 1.2129737856654094E-4 0.0 0.0 1.5162172320817617E-5 1.5162172320817617E-5 4 1.2887846472694973E-4 0.0 0.0 2.2743258481226426E-5 2.2743258481226426E-5 5 1.3645955088735856E-4 7.5810861604088085E-6 0.0 2.2743258481226426E-5 2.2743258481226426E-5 6 1.4404063704776736E-4 2.2743258481226426E-5 0.0 2.2743258481226426E-5 2.2743258481226426E-5 7 1.4404063704776736E-4 2.2743258481226426E-5 0.0 2.2743258481226426E-5 3.0324344641635234E-5 8 1.5920280936858498E-4 2.2743258481226426E-5 0.0 2.2743258481226426E-5 3.0324344641635234E-5 9 1.819460678498114E-4 2.2743258481226426E-5 0.0 3.0324344641635234E-5 3.7905430802044046E-5 10-11 2.1227041249144662E-4 2.2743258481226426E-5 0.0 3.0324344641635234E-5 4.548651696245286E-5 12-13 2.4638530021328626E-4 2.2743258481226426E-5 0.0 3.411488772183964E-5 6.064868928327047E-5 14-15 3.56311049539214E-4 2.6533801561430828E-5 0.0 4.1695973882248445E-5 7.202031852388368E-5 16-17 5.268854881484123E-4 3.0324344641635234E-5 0.0 4.548651696245285E-5 8.339194776449689E-5 18-19 5.647909189504562E-4 3.7905430802044046E-5 0.0 4.548651696245285E-5 9.47635770051101E-5 20-21 6.064868928327047E-4 3.7905430802044046E-5 0.0 4.927706004265726E-5 9.855412008531451E-5 22-23 6.785072113565883E-4 3.7905430802044046E-5 0.0 5.306760312286166E-5 1.0992574932592773E-4 24-25 7.884329606825161E-4 3.7905430802044046E-5 0.0 5.306760312286166E-5 1.1371629240613212E-4 26-27 9.286830546500791E-4 3.7905430802044046E-5 0.0 5.306760312286166E-5 1.1371629240613212E-4 28-29 0.0011030480363394817 4.548651696245285E-5 0.0 5.306760312286166E-5 1.1750683548633652E-4 30-31 0.0013077373626705195 4.548651696245285E-5 0.0 5.306760312286166E-5 1.2129737856654094E-4 32-33 0.0015692848352046236 4.927706004265726E-5 0.0 5.306760312286166E-5 1.3266900780715415E-4 34-35 0.001762602532295048 6.064868928327047E-5 0.0 5.306760312286166E-5 1.4783118012797177E-4 36-37 0.0019521296863052682 6.064868928327047E-5 0.0 6.064868928327047E-5 1.5162172320817618E-4 38-39 0.002149237926475897 6.064868928327047E-5 0.0 6.822977544367928E-5 1.5162172320817618E-4 40-41 0.002588940923779608 6.064868928327047E-5 0.0 7.202031852388368E-5 1.629933524487894E-4 42-43 0.00283153568091269 6.064868928327047E-5 0.0 7.960140468429249E-5 1.743649816894026E-4 44-45 0.0031575223858102686 6.064868928327047E-5 0.0 8.339194776449689E-5 1.743649816894026E-4 46-47 0.003638921356996228 6.064868928327047E-5 0.0 8.339194776449689E-5 1.743649816894026E-4 48-49 0.004271942051390363 6.064868928327047E-5 0.0 8.339194776449689E-5 1.78155524769607E-4 50-51 0.004844314056501229 6.064868928327047E-5 0.0 9.09730339249057E-5 1.8573661093001582E-4 52-53 0.005492496923216182 6.064868928327047E-5 0.0 9.09730339249057E-5 1.9331769709042462E-4 54-55 0.006254396082337267 6.064868928327047E-5 0.0 9.09730339249057E-5 2.1227041249144662E-4 56-57 0.007035247956859374 6.822977544367928E-5 0.0 9.09730339249057E-5 2.2364204173205984E-4 58-59 0.008168620337840491 6.822977544367928E-5 0.0 9.09730339249057E-5 2.539663863736951E-4 60-61 0.009540796932874485 6.822977544367928E-5 0.0 9.09730339249057E-5 2.842907310153303E-4 62-63 0.011261703491287285 6.822977544367928E-5 0.0 9.09730339249057E-5 2.880812740955347E-4 64-65 0.013414731960843387 6.822977544367928E-5 0.0 9.09730339249057E-5 3.1840561873716996E-4 66-67 0.01637135556340282 6.822977544367928E-5 0.0 9.09730339249057E-5 3.411488772183964E-4 68-69 0.019847283567950263 6.822977544367928E-5 0.0 9.09730339249057E-5 3.601015926194184E-4 70-71 0.025555841446738094 6.822977544367928E-5 0.0 9.09730339249057E-5 3.7147322186003163E-4 72-73 0.033027001857820974 7.581086160408809E-5 0.0 9.09730339249057E-5 3.9800702342146244E-4 74-75 0.04250715010141219 7.581086160408809E-5 0.0 9.09730339249057E-5 4.0558810958187127E-4 76-77 0.054705117733509966 7.581086160408809E-5 0.0 9.47635770051101E-5 4.207502819026889E-4 78-79 0.07081492582437868 7.581086160408809E-5 0.0 9.855412008531451E-5 4.245408249828933E-4 80-81 0.09220116988289193 7.581086160408809E-5 0.0 1.0613520624572331E-4 4.245408249828933E-4 82-83 0.11967123558513325 7.581086160408809E-5 0.0 1.1371629240613212E-4 4.3591245422350646E-4 84-85 0.15364208266992513 7.581086160408809E-5 0.0 1.2129737856654094E-4 4.4349354038391534E-4 86-87 0.1975100377371307 7.581086160408809E-5 0.0 1.2129737856654094E-4 4.889800573463681E-4 88-89 0.2515252766300434 7.581086160408809E-5 0.0 1.2887846472694973E-4 4.96561143506777E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 126870 0.0 94.30684 1 TCGACGG 129110 0.0 92.69641 2 CGACGGC 130455 0.0 91.627815 3 ACGGCGC 131845 0.0 90.60536 5 GACGGCG 132025 0.0 90.532196 4 CGGCGCG 132235 0.0 90.31145 6 TATCTCG 66065 0.0 89.57817 2 ATATCTC 68275 0.0 86.86648 1 ATCTCGA 70755 0.0 83.49283 3 TCTCGAG 70130 0.0 82.0604 4 CTCGAGG 72300 0.0 79.610596 5 TCGAGGG 72685 0.0 79.13867 6 CGAGGGC 73645 0.0 77.97687 7 AGGGCGC 74645 0.0 77.128334 9 GAGGGCG 76185 0.0 75.63785 8 CGCGCCG 205715 0.0 58.508217 9 GGCGCGC 206570 0.0 58.361206 7 GCGCGCC 206495 0.0 58.326317 8 CTCGACG 2545 0.0 57.11467 5 TCCATAT 142720 0.0 41.654022 18-19 >>END_MODULE