FastQCFastQC Report
Tue 31 May 2016
SRR1051282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13190722
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1047880.7944068565769182No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT158240.11996310740230898No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT139150.10549081392208857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG62750.083.737731
TCGACGG67800.077.55822
CGACGGC82250.063.931023
ACGGCGC97300.054.2368665
GACGGCG97300.054.1884544
TATCTCG49350.052.0738752
CGGCGCG108500.048.724676
ATCTCGA106650.040.21781
TCTCGAG120500.035.439082
TCGAGGG135450.031.8422284
CTCGAGG142400.030.188293
ATATCTC88750.029.8977491
CGAGGGC147800.029.4062755
GGGCGCG152500.028.3747468
AGGGCGC158400.027.2278987
CGCGCCG256400.025.9910079
GAGGGCG170000.025.593526
GGCGCGC264800.025.238277
GCGCGCC266050.025.119698
TCCATAT165850.016.42484918-19
Produced by FastQC (version 0.11.5)