##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051282_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13190722 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47370758022192 34.0 31.0 34.0 31.0 34.0 2 32.72345304525408 34.0 31.0 34.0 31.0 34.0 3 32.804281372922574 34.0 31.0 34.0 31.0 34.0 4 36.2749857816729 37.0 37.0 37.0 35.0 37.0 5 36.138185915827805 37.0 35.0 37.0 35.0 37.0 6 36.09967922908238 37.0 35.0 37.0 35.0 37.0 7 36.06674456485399 37.0 35.0 37.0 35.0 37.0 8 36.04801162514076 37.0 35.0 37.0 35.0 37.0 9 37.750230730357295 39.0 38.0 39.0 35.0 39.0 10-11 37.73501844705695 39.0 38.0 39.0 35.0 39.0 12-13 37.732694123945606 39.0 38.0 39.0 35.0 39.0 14-15 39.16721442541204 40.0 39.0 41.0 36.0 41.0 16-17 39.17310811341487 40.0 39.0 41.0 36.0 41.0 18-19 39.22043501485362 40.0 39.0 41.0 36.0 41.0 20-21 39.261315491297594 40.5 39.0 41.0 36.0 41.0 22-23 39.23403264051808 40.0 39.0 41.0 36.0 41.0 24-25 39.19010968467079 40.5 39.0 41.0 36.0 41.0 26-27 39.068981970812516 40.0 39.0 41.0 35.5 41.0 28-29 38.93730756360418 40.0 38.0 41.0 35.0 41.0 30-31 38.8365130430313 40.0 38.0 41.0 35.0 41.0 32-33 38.81801325962294 40.0 38.0 41.0 35.0 41.0 34-35 38.759339329568164 40.0 38.0 41.0 35.0 41.0 36-37 38.70062146711909 40.0 38.0 41.0 35.0 41.0 38-39 38.63207506761192 40.0 38.0 41.0 35.0 41.0 40-41 38.64602870108247 40.0 38.0 41.0 35.0 41.0 42-43 38.63447865097908 40.0 38.0 41.0 35.0 41.0 44-45 38.57964715653927 40.0 38.0 41.0 35.0 41.0 46-47 38.47980849721493 40.0 38.0 41.0 34.0 41.0 48-49 38.41863155784801 40.0 38.0 41.0 34.0 41.0 50-51 38.38411642668233 40.0 38.0 41.0 34.0 41.0 52-53 38.24213761763761 40.0 37.5 41.0 34.0 41.0 54-55 38.097180237745896 40.0 37.0 41.0 34.0 41.0 56-57 37.956515647892516 40.0 37.0 41.0 33.5 41.0 58-59 37.74897765262584 40.0 36.5 41.0 33.0 41.0 60-61 37.48417292851748 39.0 36.0 41.0 33.0 41.0 62-63 37.185842859852556 39.0 35.5 41.0 33.0 41.0 64-65 36.87937620093881 38.5 35.0 40.5 32.0 41.0 66-67 36.49876921066186 37.5 35.0 40.0 32.0 41.0 68-69 36.09603920846789 37.0 35.0 39.5 31.5 41.0 70-71 35.741580748953695 36.5 35.0 39.0 31.0 41.0 72-73 35.33177463674846 36.0 35.0 39.0 31.0 40.5 74-75 34.94120488628295 35.5 34.5 37.5 31.0 39.5 76-77 34.32499752477537 35.0 34.0 37.0 30.0 39.0 78-79 34.23171354835618 35.0 34.0 37.0 30.0 39.0 80-81 33.98263139045763 35.0 34.0 36.0 30.0 37.5 82-83 33.71831447133826 35.0 34.0 36.0 30.0 37.0 84-85 33.47410554933991 35.0 34.0 35.5 30.0 37.0 86-87 33.26966279783623 35.0 34.0 35.0 30.0 36.0 88-89 33.07792890336101 35.0 34.0 35.0 30.0 36.0 90-91 32.92365395161842 35.0 34.0 35.0 30.0 36.0 92-93 32.77299396500055 35.0 34.0 35.0 29.0 36.0 94-95 32.62731774651911 35.0 34.0 35.0 29.0 35.0 96-97 32.47601552818716 35.0 33.5 35.0 29.0 35.0 98-99 32.289528541348986 35.0 33.0 35.0 29.0 35.0 100-101 31.40270028433622 34.5 32.0 35.0 26.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3591666190655687 1101 2 0.34634186326591987 1101 3 0.32792972831303047 1101 4 0.22456395094640413 1101 5 0.27070144286197717 1101 6 0.2963013661413356 1101 7 0.3044587469459401 1101 8 0.2987058762110948 1101 9 0.40627487485154745 1101 10-11 0.36349034069541375 1101 12-13 0.3446497218420532 1101 14-15 0.43580545401299275 1101 16-17 0.45541507545310367 1101 18-19 0.4236488473829354 1101 20-21 0.36635541576512765 1101 22-23 0.4119489373949392 1101 24-25 0.3611814930867041 1101 26-27 0.3836344523232569 1101 28-29 0.42158519405735717 1101 30-31 0.420906970720786 1101 32-33 0.43145809373599775 1101 34-35 0.39339752472189815 1101 36-37 0.3418539094850388 1101 38-39 0.3084371405796631 1101 40-41 0.3793947975131857 1101 42-43 0.38122575428135974 1101 44-45 0.4113539107586277 1101 46-47 0.41760988211893135 1101 48-49 0.4022419634171186 1101 50-51 0.41476014253410654 1101 52-53 0.3660986104084216 1101 54-55 0.39466595540540794 1101 56-57 0.3871499843152222 1101 58-59 0.3493688393858534 1101 60-61 0.36518505311961036 1101 62-63 0.31240709140566025 1101 64-65 0.32205521841139984 1101 66-67 0.38953604236176176 1101 68-69 0.3925241124865977 1101 70-71 0.3468297088519492 1101 72-73 0.3407254661702339 1101 74-75 0.4047266872751294 1101 76-77 0.2575983316073973 1101 78-79 0.3160634754284075 1101 80-81 0.2775741812138648 1101 82-83 0.2885431227859456 1101 84-85 0.24519825225845437 1101 86-87 0.18661317537993227 1101 88-89 0.16270744726638497 1101 90-91 0.17144027610252977 1101 92-93 0.1818154296308805 1101 94-95 0.10420556960210803 1101 96-97 0.1021876936286219 1101 98-99 0.0915323947933615 1101 100-101 0.23471512613568635 1102 1 0.31383568411344953 1102 2 0.29527338851765705 1102 3 0.2797962547820916 1102 4 0.1902907967282772 1102 5 0.20101930523472333 1102 6 0.23281472225181687 1102 7 0.21812621100770713 1102 8 0.23489131819681575 1102 9 0.3301871331388426 1102 10-11 0.28745008335227595 1102 12-13 0.27799972296924125 1102 14-15 0.3636925838415195 1102 16-17 0.32089161033705693 1102 18-19 0.3752742895467662 1102 20-21 0.3172965293483401 1102 22-23 0.27085005052185807 1102 24-25 0.29228543916369176 1102 26-27 0.30169481950181876 1102 28-29 0.35768904814389657 1102 30-31 0.3643879815160318 1102 32-33 0.3101343225866344 1102 34-35 0.2691778178145867 1102 36-37 0.28432327594187967 1102 38-39 0.27709720828391227 1102 40-41 0.3275995564190808 1102 42-43 0.3278131713314423 1102 44-45 0.2967808501440601 1102 46-47 0.304678372384096 1102 48-49 0.32827006645964474 1102 50-51 0.3327493413015219 1102 52-53 0.3027966903016974 1102 54-55 0.2911690465194354 1102 56-57 0.30089001787001735 1102 58-59 0.2836373055682131 1102 60-61 0.26923066924754124 1102 62-63 0.2748959285878101 1102 64-65 0.2509501246439996 1102 66-67 0.2578393858141439 1102 68-69 0.2593075372558218 1102 70-71 0.30492564404630684 1102 72-73 0.305781290826836 1102 74-75 0.261906767197452 1102 76-77 0.1764258210316143 1102 78-79 0.2885130033335841 1102 80-81 0.21489215798533934 1102 82-83 0.2073040331606748 1102 84-85 0.25705231377285287 1102 86-87 0.2603211191244412 1102 88-89 0.19744029633523752 1102 90-91 0.19366125402254397 1102 92-93 0.2719425773708508 1102 94-95 0.23825163272145033 1102 96-97 0.2524586168005456 1102 98-99 0.27741534083108377 1102 100-101 0.4423981937742205 1103 1 0.3284135422790726 1103 2 0.30558048569220375 1103 3 0.29579234872433346 1103 4 0.18496667956542723 1103 5 0.22514592349668305 1103 6 0.21618611069875016 1103 7 0.2358191450250473 1103 8 0.24466258506088678 1103 9 0.29907862728681067 1103 10-11 0.3181041263486506 1103 12-13 0.3019576612598911 1103 14-15 0.4019188024693534 1103 16-17 0.38539884394126744 1103 18-19 0.37251296008794554 1103 20-21 0.34648487055724786 1103 22-23 0.3357020693594137 1103 24-25 0.31527484365491887 1103 26-27 0.3607890287685933 1103 28-29 0.2643558732895457 1103 30-31 0.3285171306126884 1103 32-33 0.3516297760957201 1103 34-35 0.34140425158584264 1103 36-37 0.22228540869348734 1103 38-39 0.2406758224119514 1103 40-41 0.2741948488775634 1103 42-43 0.27605426115894005 1103 44-45 0.27823896383660696 1103 46-47 0.296539235441017 1103 48-49 0.3303162815278782 1103 50-51 0.3603533587380028 1103 52-53 0.35497409796369794 1103 54-55 0.344586913401983 1103 56-57 0.37827476215682054 1103 58-59 0.37900027964588645 1103 60-61 0.36617713250041817 1103 62-63 0.3761902024270114 1103 64-65 0.39424647664916534 1103 66-67 0.35896628144372045 1103 68-69 0.38532533118765144 1103 70-71 0.3800483737193119 1103 72-73 0.38889163133141835 1103 74-75 0.38929158518076434 1103 76-77 0.29323670273815594 1103 78-79 0.39083233367511383 1103 80-81 0.40506067078525376 1103 82-83 0.40825563859902303 1103 84-85 0.38508879996126666 1103 86-87 0.30789741017054695 1103 88-89 0.35893927140367055 1103 90-91 0.34205270236530083 1103 92-93 0.375121566992469 1103 94-95 0.42380068561337936 1103 96-97 0.42460842866826454 1103 98-99 0.4293938880166479 1103 100-101 0.5474475693014931 1104 1 0.27365713629634314 1104 2 0.2384795157224957 1104 3 0.20846376703863 1104 4 0.15762962994325846 1104 5 0.16610551379937277 1104 6 0.19157641361215383 1104 7 0.22172036302167442 1104 8 0.1890293550996205 1104 9 0.25812696080878794 1104 10-11 0.2505369854489672 1104 12-13 0.26033099441133345 1104 14-15 0.3147971356111583 1104 16-17 0.33491828785901134 1104 18-19 0.29938505359622525 1104 20-21 0.25254698858555713 1104 22-23 0.2746652588201215 1104 24-25 0.27297589699482216 1104 26-27 0.2914066400871249 1104 28-29 0.2771884650674252 1104 30-31 0.2664777164355385 1104 32-33 0.2745824365213281 1104 34-35 0.27954800041943173 1104 36-37 0.2576269886361118 1104 38-39 0.24759597408390022 1104 40-41 0.26054917754837703 1104 42-43 0.26950762120459615 1104 44-45 0.28646167060489347 1104 46-47 0.2641015475450388 1104 48-49 0.29042700513406317 1104 50-51 0.23085726543320817 1104 52-53 0.24358044601071072 1104 54-55 0.29596526968033743 1104 56-57 0.33732853974538557 1104 58-59 0.31928871452991814 1104 60-61 0.2954721698115961 1104 62-63 0.33984113697637497 1104 64-65 0.3151391638062222 1104 66-67 0.32843174054650603 1104 68-69 0.367652565174474 1104 70-71 0.2967557285343432 1104 72-73 0.3435162346831575 1104 74-75 0.3451007128449177 1104 76-77 0.225767828272339 1104 78-79 0.30577935511315957 1104 80-81 0.30827362737672814 1104 82-83 0.2771846669596272 1104 84-85 0.36935289307609054 1104 86-87 0.35537448287579565 1104 88-89 0.3285233834413148 1104 90-91 0.3355333358183685 1104 92-93 0.3572825136903859 1104 94-95 0.37004359068446746 1104 96-97 0.37068163887166605 1104 98-99 0.4276235697556672 1104 100-101 0.6052317736573087 1105 1 0.15371073813350478 1105 2 0.1836880932990823 1105 3 0.1379581472096021 1105 4 0.09792236021301903 1105 5 0.12371696042546887 1105 6 0.13251655588175026 1105 7 0.09900917429506961 1105 8 0.10908425204581818 1105 9 0.1711169578284384 1105 10-11 0.1679192280611872 1105 12-13 0.18728914760366422 1105 14-15 0.266951697343238 1105 16-17 0.23982107718894952 1105 18-19 0.14831439747119646 1105 20-21 0.13048603099227307 1105 22-23 0.11942025429793546 1105 24-25 0.12913001639461186 1105 26-27 0.1775413132555812 1105 28-29 0.17962098089065393 1105 30-31 0.19907891085741625 1105 32-33 0.1587120956404391 1105 34-35 0.143836081479364 1105 36-37 0.11568113719811635 1105 38-39 0.12334118730213817 1105 40-41 0.1181219045117885 1105 42-43 0.09629088568813415 1105 44-45 0.062401742507489644 1105 46-47 0.11988697505660895 1105 48-49 0.15247181305620927 1105 50-51 0.15752601430978075 1105 52-53 0.15622155633422352 1105 54-55 0.15338852467235142 1105 56-57 0.10162487333981574 1105 58-59 0.12252373923051607 1105 60-61 0.17524566201163339 1105 62-63 0.163284556655654 1105 64-65 0.22478210117763808 1105 66-67 0.20375502790732725 1105 68-69 0.21235779158963908 1105 70-71 0.21990797136211881 1105 72-73 0.2767199309278894 1105 74-75 0.24419241297747618 1105 76-77 0.13720794319678475 1105 78-79 0.21722481882008537 1105 80-81 0.20205450312339934 1105 82-83 0.26753650550674024 1105 84-85 0.25000584803568415 1105 86-87 0.2626550977301392 1105 88-89 0.240074228406435 1105 90-91 0.21960715115434937 1105 92-93 0.2627262025457 1105 94-95 0.2600321618371737 1105 96-97 0.2097847081731814 1105 98-99 0.24122017927780348 1105 100-101 0.38854636472618864 1106 1 0.04891771972219772 1106 2 0.05571740469729747 1106 3 0.07046146345641802 1106 4 0.0557581694306748 1106 5 0.0987671431561239 1106 6 0.11160939465177933 1106 7 0.11689261452669086 1106 8 0.05530622138766006 1106 9 0.12240486804063266 1106 10-11 0.08469567767976827 1106 12-13 0.12271837480772518 1106 14-15 0.1710741358885528 1106 16-17 0.14175901687007553 1106 18-19 0.08311383141570161 1106 20-21 0.07876393783524804 1106 22-23 0.09516352608083878 1106 24-25 0.07417857129125593 1106 26-27 0.06699799787384109 1106 28-29 0.07442251956698698 1106 30-31 0.10632742883782242 1106 32-33 0.10347362788878911 1106 34-35 0.12391495349222481 1106 36-37 0.08109006177384259 1106 38-39 0.040205524003489757 1106 40-41 0.07631184719019757 1106 42-43 0.06924159801792484 1106 44-45 0.03179508526303465 1106 46-47 0.08658792969144002 1106 48-49 0.10033899602927221 1106 50-51 0.10584153332418111 1106 52-53 0.10398082723715163 1106 54-55 0.11571662993094378 1106 56-57 0.14688642712955158 1106 58-59 0.07405032625939612 1106 60-61 0.04976638804855327 1106 62-63 0.018862942096816937 1106 64-65 0.0918507579480945 1106 66-67 0.1196308447250729 1106 68-69 0.15019786952706937 1106 70-71 0.1383768905541629 1106 72-73 0.1504188307346368 1106 74-75 0.1321428102649378 1106 76-77 -0.016703680710932645 1106 78-79 0.1447032355717539 1106 80-81 0.10997468516145403 1106 82-83 0.08611239827569506 1106 84-85 0.11830938933692892 1106 86-87 0.13103370790826574 1106 88-89 0.1103666525594349 1106 90-91 0.03586957753116593 1106 92-93 0.06952226178902521 1106 94-95 0.09860802823389037 1106 96-97 0.06896569481803283 1106 98-99 0.04277312429194069 1106 100-101 0.3199135449765578 1107 1 -0.006883208448343225 1107 2 0.04042214583166981 1107 3 0.016550284148095784 1107 4 0.009233280688178525 1107 5 0.014304909528490839 1107 6 0.004807321805962772 1107 7 0.038895026801789356 1107 8 0.03218895643853159 1107 9 -0.0027139878586197597 1107 10-11 0.05126144835973179 1107 12-13 0.017175036184625014 1107 14-15 0.052918398975833725 1107 16-17 0.03209769340455182 1107 18-19 -0.020300955390680997 1107 20-21 -0.051220996576603284 1107 22-23 -0.06847320832338255 1107 24-25 -0.08108966702211262 1107 26-27 -0.055797875163769106 1107 28-29 -0.07187850032504173 1107 30-31 -0.10271498034382631 1107 32-33 -0.05882357448284381 1107 34-35 -0.13418715926559344 1107 36-37 -0.145836410793585 1107 38-39 -0.19649469980416256 1107 40-41 -0.25136234212327935 1107 42-43 -0.13515348348384038 1107 44-45 -0.1129317491403583 1107 46-47 -0.08326316268554734 1107 48-49 -0.11622831347985141 1107 50-51 -0.028939360045207252 1107 52-53 0.03507281662916739 1107 54-55 0.01678545119901287 1107 56-57 -0.03178212525465085 1107 58-59 -0.03452727516332743 1107 60-61 -0.06759178768817975 1107 62-63 -0.0500046256721518 1107 64-65 -0.03811585292092445 1107 66-67 -0.008772738397595958 1107 68-69 0.003739683782903569 1107 70-71 -0.016216105889611754 1107 72-73 0.003569557412028246 1107 74-75 -0.0696344906735078 1107 76-77 -0.25166846882299154 1107 78-79 -0.06877245670642651 1107 80-81 -0.051460846358757806 1107 82-83 -0.1181958982610638 1107 84-85 -0.05384813562938007 1107 86-87 0.051662308203226814 1107 88-89 -0.04276880035801156 1107 90-91 -0.05162940192930421 1107 92-93 -0.010830963941465654 1107 94-95 -0.05263014415590561 1107 96-97 -0.08223415644600607 1107 98-99 -0.09714915143578651 1107 100-101 0.13610597585718054 1108 1 -0.11599577987604448 1108 2 -0.07772224680004314 1108 3 -0.10492043700587317 1108 4 -0.06565431821509549 1108 5 -0.08892226941561887 1108 6 -0.06451552479761347 1108 7 -0.11212990637802278 1108 8 -0.09467276131884717 1108 9 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40-41 -0.7152387771638544 2308 42-43 -0.7270228328177097 2308 44-45 -0.6697030868709604 2308 46-47 -0.5930033569602315 2308 48-49 -0.529567264336464 2308 50-51 -0.46924275767572965 2308 52-53 -0.48659188330304914 2308 54-55 -0.4937435798478802 2308 56-57 -0.6310521515207412 2308 58-59 -0.6054165429158331 2308 60-61 -0.754552217272412 2308 62-63 -0.9021974881077668 2308 64-65 -0.7141152181999999 2308 66-67 -0.8536906531832074 2308 68-69 -0.8710648566023877 2308 70-71 -1.2250282439352418 2308 72-73 -0.9948221585544204 2308 74-75 -0.9679616126324646 2308 76-77 -1.3201608427748823 2308 78-79 -1.1318278622477322 2308 80-81 -1.1009367064349078 2308 82-83 -1.2049599057652287 2308 84-85 -1.2166724676673226 2308 86-87 -1.32317782221698 2308 88-89 -1.3222019516396202 2308 90-91 -1.2586949479040754 2308 92-93 -1.184841866213869 2308 94-95 -1.2049942204190813 2308 96-97 -1.2442746409689676 2308 98-99 -1.4456304592879619 2308 100-101 -1.310118173329215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 4.0 11 87.0 12 345.0 13 971.0 14 2479.0 15 4863.0 16 7754.0 17 12393.0 18 21948.0 19 36949.0 20 35160.0 21 32112.0 22 32187.0 23 34571.0 24 40351.0 25 50549.0 26 64456.0 27 83694.0 28 106859.0 29 133719.0 30 170038.0 31 218892.0 32 286022.0 33 384719.0 34 558765.0 35 900391.0 36 1488497.0 37 3205397.0 38 4425298.0 39 850783.0 40 469.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 24.794135460760238 32.418884905503305 22.215885535301684 20.571094098434777 2 23.789354475275054 29.743368371527218 26.2201561840551 20.247120969142628 3 23.373914939423255 27.07330806134024 27.28213822804275 22.27063877119375 4 24.42889084635576 27.048826061935255 26.75108185676119 21.771201234947803 5 24.406516964261073 28.474646327081228 25.131739390570562 21.987097318087134 6 24.303688607795692 27.247871647965894 24.08517896139423 24.363260782844183 7 24.406071176392015 27.154487828641983 25.574445432175736 22.86499556279027 8 23.734159509994978 26.929405380539446 25.40017900460642 23.936256104859158 9 23.496302931712155 26.394688630387332 25.81579689117851 24.293211546722006 10-11 23.73025904116545 26.968258447111538 25.72691623703388 23.574566274689136 12-13 23.536773002436977 26.732247637950522 26.159659333137817 23.57132002647468 14-15 23.31414080290677 26.807619021915556 26.19519234807617 23.683047827101504 16-17 23.490429856461304 27.010026565010893 26.037270010543068 23.462273567984738 18-19 23.187578360001826 26.88281960608373 26.47240992570384 23.457192108210602 20-21 22.935571684400596 26.97233707146584 26.51898432853031 23.573106915603255 22-23 22.887011037000097 26.914125701383142 26.930197604043205 23.26866565757356 24-25 22.876374773117046 26.606754353552443 27.43079946647348 23.08607140685703 26-27 22.848279267806568 26.642578776203457 27.5980761326029 22.911065823387077 28-29 22.84495117098215 26.76563496675921 27.580105167859653 22.80930869439899 30-31 22.90240078794843 26.675597699017136 27.56416617724397 22.857835335790465 32-33 22.865211623745843 26.718567793332316 27.5858023541092 22.830418228812647 34-35 22.8435827849302 26.838530900734625 27.547707396153143 22.770178918182037 36-37 22.88163225636929 26.825999365311464 27.42062185830313 22.871746520016114 38-39 22.83066461411286 26.882137308329295 27.43877855965731 22.84841951790054 40-41 22.946522919098232 27.036334281592 27.136746167432975 22.8803966318768 42-43 22.942010880986295 27.04469946892951 27.08249120062929 22.930798449454905 44-45 22.90818518293471 27.141382231307297 27.07762860150297 22.872803984255025 46-47 22.955249583930122 27.310361934736864 26.877625004235938 22.856763477097072 48-49 22.98515956897583 27.311514866282526 26.743983384685084 22.95934218005656 50-51 22.966297826608734 27.327473052650188 26.79932531365607 22.906903807085012 52-53 22.94492674472254 27.393038076308486 26.75298971504365 22.909045463925327 54-55 22.954652347908937 27.361736845823987 26.75866874853495 22.92494205773212 56-57 22.95233110492309 27.34013968457671 26.808421133070908 22.89910807742929 58-59 22.951741209499165 27.47484670278702 26.740663660714098 22.832748426999714 60-61 22.945578718132335 27.412532839369973 26.74511296652298 22.896775475974703 62-63 22.939843626451985 27.44620802409451 26.755385338270337 22.858563011183165 64-65 22.90482658947706 27.530468006224375 26.729958375288327 22.834747029010238 66-67 22.91692979353215 27.49884350530623 26.701203315481898 22.883023385679724 68-69 22.871624374891258 27.599480088441062 26.673210415970942 22.855685120696734 70-71 22.86978940991131 27.660414758996676 26.692533588290345 22.777262242801672 72-73 22.87591039559588 27.610809005260357 26.666663886900306 22.846616712243463 74-75 22.827902554301915 27.697757706993798 26.685965186398334 22.788374552305957 76-77 22.874855679421994 27.787958572133455 26.58151643585798 22.755669312586573 78-79 22.816352759733558 27.72205255890595 26.606684355184022 22.854910326176466 80-81 22.79819694736803 27.743597503631207 26.65733622763441 22.800869321366353 82-83 22.793323106143067 27.8927402730471 26.583707427103032 22.7302291937068 84-85 22.77367931593732 27.83693931514434 26.61369306499622 22.775688303922127 86-87 22.772675009731273 27.91775185222122 26.58866559674753 22.720907541299972 88-89 22.786628488716527 28.00081921443734 26.533439069561627 22.67911322728451 90-91 22.80484655483703 27.942519608243376 26.475697119547846 22.776936717371747 92-93 22.747860807164034 28.032882649695168 26.50055651100822 22.718700032132574 94-95 22.783010587555523 28.090582432115507 26.43221072597397 22.694196254355 96-97 22.73747670661688 28.109367430159843 26.41122147371982 22.74193438950346 98-99 22.740846073695984 28.18466359135095 26.374320881256264 22.70016945369681 100-101 22.77212864419973 28.238405966991188 26.262967836675717 22.72649755213337 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 84167.0 1 47904.5 2 8292.0 3 4263.5 4 3219.0 5 2605.5 6 2307.5 7 2189.5 8 2102.0 9 2080.0 10 2108.5 11 2231.0 12 2818.0 13 4129.5 14 5306.5 15 5462.0 16 12711.5 17 13031.5 18 5737.5 19 5938.5 20 7144.0 21 9453.5 22 12682.0 23 16760.0 24 21830.5 25 28012.5 26 35644.5 27 45774.5 28 57876.5 29 73065.5 30 92122.0 31 112978.5 32 137957.5 33 171649.0 34 209926.5 35 248204.0 36 289094.5 37 330003.5 38 361744.5 39 388079.5 40 414895.5 41 438905.5 42 465429.0 43 499381.0 44 537533.5 45 571030.5 46 581885.5 47 576334.0 48 570440.0 49 563906.0 50 550831.5 51 529650.5 52 506511.0 53 485038.0 54 456600.5 55 419776.0 56 379208.5 57 334162.0 58 291861.0 59 248969.5 60 205305.0 61 169258.0 62 138017.0 63 107890.0 64 82096.5 65 61259.0 66 45161.5 67 33423.5 68 24903.5 69 18663.0 70 14125.5 71 10829.5 72 8466.5 73 6478.5 74 4845.5 75 3619.0 76 2665.0 77 1831.5 78 1212.0 79 762.5 80 438.0 81 250.5 82 141.0 83 78.0 84 49.5 85 34.5 86 19.5 87 7.5 88 5.0 89 3.5 90 4.0 91 2.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10226127121775441 2 0.0019635013155458814 3 7.50527529880472E-4 4 0.0015541226628838058 5 0.002168190641876919 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.1695973882248445E-5 14-15 0.0 16-17 4.776084281057549E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 8.339194776449689E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.9800702342146244E-4 42-43 4.548651696245285E-5 44-45 7.618991591210852E-4 46-47 2.1985149865185545E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 4.548651696245285E-5 56-57 2.2743258481226426E-5 58-59 4.548651696245285E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.2887846472694973E-4 70-71 1.1371629240613213E-5 72-73 0.0012508792164674534 74-75 5.53419289709843E-4 76-77 2.691285586945127E-4 78-79 4.207502819026888E-4 80-81 0.001538960490562988 82-83 5.117233158275946E-4 84-85 7.5810861604088085E-6 86-87 4.1695973882248445E-5 88-89 2.767096448549215E-4 90-91 2.463853002132863E-4 92-93 4.359124542235065E-4 94-95 0.0021492379264758973 96-97 9.47635770051101E-5 98-99 7.429464437200633E-4 100-101 0.001171277811783161 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3190722E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.41980736023498 #Duplication Level Percentage of deduplicated Percentage of total 1 78.00034045277064 44.787645228308335 2 12.550025394490467 14.412400810353992 3 4.060180627855754 6.994043684977058 4 1.868665177775572 4.291935781146104 5 0.9701233962454089 2.7852149264034143 6 0.5883663015079253 2.02703278139034 7 0.37837496436402007 1.5208352294602465 8 0.26861817141263383 1.233920292477761 9 0.20027347484238614 1.034969791033321 >10 0.952786003560243 9.883143405352355 >50 0.09740642171416752 4.015423134025211 >100 0.06436304835415614 5.659466646753778 >500 3.448233091561108E-4 0.1337623888375136 >1k 7.904508173516856E-5 0.15489251534007906 >5k 1.317417890214496E-5 0.04767203450179124 >10k+ 3.9522536706434875E-5 1.017641349638602 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104788 0.7944068565769182 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15824 0.11996310740230898 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 13915 0.10549081392208857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.855412008531451E-5 0.0 0.0 0.0 0.0 2 9.855412008531451E-5 7.5810861604088085E-6 0.0 0.0 0.0 3 1.0613520624572333E-4 1.5162172320817617E-5 0.0 0.0 7.5810861604088085E-6 4 1.1371629240613212E-4 1.5162172320817617E-5 0.0 0.0 2.2743258481226426E-5 5 1.2129737856654094E-4 1.5162172320817617E-5 0.0 0.0 2.2743258481226426E-5 6 1.2129737856654094E-4 1.5162172320817617E-5 0.0 0.0 2.2743258481226426E-5 7 1.2887846472694973E-4 1.5162172320817617E-5 0.0 0.0 2.2743258481226426E-5 8 1.3645955088735856E-4 1.5162172320817617E-5 0.0 0.0 3.0324344641635234E-5 9 1.6678389552899378E-4 1.5162172320817617E-5 0.0 0.0 3.7905430802044046E-5 10-11 1.8573661093001582E-4 1.5162172320817617E-5 0.0 0.0 3.7905430802044046E-5 12-13 2.0468932633103783E-4 2.2743258481226426E-5 0.0 7.5810861604088085E-6 4.1695973882248445E-5 14-15 3.032434464163523E-4 3.0324344641635234E-5 0.0 1.5162172320817617E-5 4.927706004265726E-5 16-17 5.193044019880034E-4 3.0324344641635234E-5 0.0 2.2743258481226426E-5 6.822977544367928E-5 18-19 5.610003758702519E-4 3.7905430802044046E-5 0.0 2.6533801561430828E-5 8.339194776449689E-5 20-21 6.064868928327047E-4 4.548651696245286E-5 0.0 3.0324344641635234E-5 9.855412008531451E-5 22-23 6.74716668276384E-4 5.306760312286166E-5 0.0 3.0324344641635234E-5 1.0613520624572333E-4 24-25 7.732707883616985E-4 5.306760312286166E-5 0.0 3.0324344641635234E-5 1.0992574932592773E-4 26-27 9.173114254094659E-4 5.306760312286166E-5 0.0 3.0324344641635234E-5 1.2129737856654094E-4 28-29 0.0010689331486176418 5.306760312286166E-5 0.0 3.0324344641635234E-5 1.3266900780715415E-4 30-31 0.001284994104189293 5.306760312286166E-5 0.0 3.0324344641635234E-5 1.4025009396756297E-4 32-33 0.001546541576723397 5.306760312286166E-5 7.5810861604088085E-6 3.0324344641635234E-5 1.4404063704776736E-4 34-35 0.0017284876445732085 5.306760312286166E-5 7.5810861604088085E-6 3.7905430802044046E-5 1.4783118012797177E-4 36-37 0.001921805341663633 5.306760312286166E-5 7.5810861604088085E-6 3.7905430802044046E-5 1.5920280936858498E-4 38-39 0.0020923797802728313 5.685814620306607E-5 7.5810861604088085E-6 3.7905430802044046E-5 1.629933524487894E-4 40-41 0.0025207111483359287 6.064868928327047E-5 7.5810861604088085E-6 3.7905430802044046E-5 1.705744386091982E-4 42-43 0.0027670964485492153 6.443923236347488E-5 7.5810861604088085E-6 3.7905430802044046E-5 1.743649816894026E-4 44-45 0.003066549351885363 6.822977544367928E-5 7.5810861604088085E-6 3.7905430802044046E-5 1.819460678498114E-4 46-47 0.003506252349189074 6.822977544367928E-5 7.5810861604088085E-6 3.7905430802044046E-5 1.8573661093001582E-4 48-49 0.0041241108712623915 6.822977544367928E-5 7.5810861604088085E-6 3.7905430802044046E-5 2.1227041249144662E-4 50-51 0.0046699490748118265 7.581086160408809E-5 7.5810861604088085E-6 3.7905430802044046E-5 2.3501367097267305E-4 52-53 0.005325713027687189 9.09730339249057E-5 7.5810861604088085E-6 3.7905430802044046E-5 2.4259475713308187E-4 54-55 0.0060648689283270465 9.09730339249057E-5 7.5810861604088085E-6 3.7905430802044046E-5 2.501758432934907E-4 56-57 0.00684193025976895 9.09730339249057E-5 7.5810861604088085E-6 3.7905430802044046E-5 2.539663863736951E-4 58-59 0.007967721554589658 9.09730339249057E-5 7.5810861604088085E-6 4.548651696245286E-5 2.5775692945389947E-4 60-61 0.009339898149623653 9.09730339249057E-5 7.5810861604088085E-6 5.306760312286166E-5 2.5775692945389947E-4 62-63 0.011091129052678086 9.09730339249057E-5 7.5810861604088085E-6 6.064868928327047E-5 2.6912855869451273E-4 64-65 0.013236576436073781 9.47635770051101E-5 7.5810861604088085E-6 6.443923236347488E-5 2.729191017747171E-4 66-67 0.016185618952472808 9.855412008531451E-5 7.5810861604088085E-6 6.822977544367928E-5 2.842907310153303E-4 68-69 0.019661546957020243 9.855412008531451E-5 7.5810861604088085E-6 7.202031852388368E-5 2.9187181717573916E-4 70-71 0.02539663863736951 9.855412008531451E-5 7.5810861604088085E-6 7.581086160408809E-5 2.9945290333614793E-4 72-73 0.032928447737735664 9.855412008531451E-5 7.5810861604088085E-6 7.960140468429249E-5 3.1082453257676114E-4 74-75 0.04229867023200094 9.855412008531451E-5 7.5810861604088085E-6 9.09730339249057E-5 3.1840561873716996E-4 76-77 0.054394293200933204 1.0234466316551893E-4 7.5810861604088085E-6 9.47635770051101E-5 3.3356779105798756E-4 78-79 0.07046240531791967 1.0613520624572333E-4 7.5810861604088085E-6 1.0613520624572333E-4 3.56311049539214E-4 80-81 0.0916629127655029 1.0613520624572333E-4 7.5810861604088085E-6 1.0613520624572333E-4 3.56311049539214E-4 82-83 0.11912918792466401 1.0613520624572333E-4 7.5810861604088085E-6 1.1371629240613214E-4 3.56311049539214E-4 84-85 0.15306971066481423 1.0613520624572333E-4 7.5810861604088085E-6 1.2129737856654094E-4 3.638921356996228E-4 86-87 0.1966836993456461 1.1371629240613212E-4 7.5810861604088085E-6 1.2887846472694973E-4 3.790543080204404E-4 88-89 0.25042222859370394 1.1750683548633652E-4 7.5810861604088085E-6 1.3645955088735856E-4 3.790543080204404E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 6275 0.0 83.73773 1 TCGACGG 6780 0.0 77.5582 2 CGACGGC 8225 0.0 63.93102 3 ACGGCGC 9730 0.0 54.236866 5 GACGGCG 9730 0.0 54.188454 4 TATCTCG 4935 0.0 52.073875 2 CGGCGCG 10850 0.0 48.72467 6 ATCTCGA 10665 0.0 40.2178 1 TCTCGAG 12050 0.0 35.43908 2 TCGAGGG 13545 0.0 31.842228 4 CTCGAGG 14240 0.0 30.18829 3 ATATCTC 8875 0.0 29.897749 1 CGAGGGC 14780 0.0 29.406275 5 GGGCGCG 15250 0.0 28.374746 8 AGGGCGC 15840 0.0 27.227898 7 CGCGCCG 25640 0.0 25.991007 9 GAGGGCG 17000 0.0 25.59352 6 GGCGCGC 26480 0.0 25.23827 7 GCGCGCC 26605 0.0 25.11969 8 TCCATAT 16585 0.0 16.424849 18-19 >>END_MODULE