FastQCFastQC Report
Tue 31 May 2016
SRR1051281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23594202
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT20463828.673240993698366No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT1191820.5051325745197909No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT672600.2850700354265001No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346200.14673096381899248No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT239620.10155884907656551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1969150.094.15611
TATCTCG2501100.092.54192
TCGACGG2018900.091.908832
ATATCTC2527200.091.659461
CGACGGC2039100.090.82833
ACGGCGC2061200.089.8774955
GACGGCG2063950.089.7853554
CGGCGCG2076550.089.203966
ATCTCGA2593200.089.0958253
TCTCGAG2608300.087.716884
CTCGAGG2656650.086.181255
TCGAGGG2661750.085.942976
CGAGGGC2682750.085.206487
AGGGCGC2711950.084.3100749
GAGGGCG2734850.083.744798
CTCGACG41350.054.791855
GGGCGCG2735150.041.91043510-11
CCATATT2224050.041.68453618-19
TCCATAT2207650.041.6445818-19
ATCCATA2259650.040.82724416-17
Produced by FastQC (version 0.11.5)