##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051281_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23594202 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.18685916989267 31.0 31.0 33.0 28.0 34.0 2 31.447456921831897 31.0 31.0 34.0 28.0 34.0 3 31.699259970733486 31.0 31.0 34.0 30.0 34.0 4 35.193117868533975 37.0 35.0 37.0 32.0 37.0 5 35.247027341717256 37.0 35.0 37.0 32.0 37.0 6 35.28121044314192 37.0 35.0 37.0 32.0 37.0 7 35.30452591700283 37.0 35.0 37.0 32.0 37.0 8 35.269060509018274 37.0 35.0 37.0 32.0 37.0 9 36.83940105285188 39.0 37.0 39.0 32.0 39.0 10-11 36.94587348196816 39.0 37.0 39.0 33.0 39.0 12-13 36.896680464124195 39.0 37.0 39.0 33.0 39.0 14-15 38.12970139867413 40.0 38.0 41.0 33.0 41.0 16-17 37.90108737307581 40.0 37.5 41.0 32.5 41.0 18-19 37.91589980453672 40.0 37.5 41.0 32.5 41.0 20-21 37.89142090925559 40.0 37.5 41.0 32.0 41.0 22-23 37.97388985649949 40.0 38.0 41.0 33.0 41.0 24-25 38.10475115878045 40.0 38.0 41.0 33.0 41.0 26-27 38.19165085557884 40.0 38.0 41.0 33.0 41.0 28-29 37.98314802085699 39.5 38.0 41.0 33.0 41.0 30-31 37.3278956626717 39.0 36.0 40.5 32.0 41.0 32-33 36.69778248910474 38.0 35.0 40.0 31.5 41.0 34-35 36.4913095598656 38.0 35.0 40.0 31.0 41.0 36-37 36.35957276707218 38.0 35.0 40.0 31.0 41.0 38-39 36.12371738616123 38.0 35.0 40.0 30.0 41.0 40-41 35.2822496179358 38.0 35.0 40.0 24.5 41.0 42-43 35.351648955959604 38.0 35.0 40.0 25.0 41.0 44-45 35.22416077051472 38.0 34.0 40.0 24.5 41.0 46-47 35.17009596255894 38.0 34.0 40.0 24.5 41.0 48-49 34.99877474135383 38.0 33.0 40.0 24.0 41.0 50-51 34.911177373152945 37.5 33.0 40.0 24.0 41.0 52-53 34.91083834918426 38.0 33.0 40.0 24.5 41.0 54-55 34.566505364326375 37.5 33.0 40.0 24.0 41.0 56-57 34.214096857355045 37.0 33.0 40.0 23.5 41.0 58-59 33.77283677574685 37.0 33.0 40.0 22.5 41.0 60-61 33.43751045277988 36.5 33.0 40.0 20.5 41.0 62-63 33.007278673803 36.0 32.0 40.0 18.0 41.0 64-65 32.6580275527013 36.0 32.0 39.0 17.5 41.0 66-67 32.17561765810092 35.0 31.5 39.0 11.5 41.0 68-69 31.609038462076406 35.0 31.0 39.0 7.5 40.0 70-71 31.12610723176821 35.0 31.0 38.0 4.5 40.0 72-73 30.59615820022224 35.0 30.0 37.0 2.0 39.5 74-75 30.11802719583396 35.0 30.0 37.0 2.0 39.0 76-77 29.557620808705458 35.0 29.0 36.0 2.0 39.0 78-79 29.19900050020764 34.0 29.0 36.0 2.0 37.5 80-81 28.774902791796052 34.0 29.0 35.0 2.0 37.0 82-83 28.360663607101436 34.0 29.0 35.0 2.0 37.0 84-85 28.002248327788326 34.0 27.5 35.0 2.0 36.0 86-87 27.672960691783516 34.0 27.0 35.0 2.0 36.0 88-89 27.345815933931565 34.0 26.5 35.0 2.0 36.0 90-91 27.037888482094033 34.0 25.5 35.0 2.0 35.0 92-93 26.779936231791183 34.0 25.0 35.0 2.0 35.0 94-95 26.480098224979173 34.0 25.0 35.0 2.0 35.0 96-97 26.11941154017415 34.0 24.0 35.0 2.0 35.0 98-99 25.708170528505264 34.0 21.5 35.0 2.0 35.0 100-101 24.522057071478834 32.0 9.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.0739542064848564 1101 2 -0.013392615455813939 1101 3 0.09050432704707134 1101 4 -0.04353914206945575 1101 5 0.061053028335827264 1101 6 0.024707688319196563 1101 7 0.08856656429061616 1101 8 0.1870570218784522 1101 9 0.25462813338852897 1101 10-11 0.3375047830858833 1101 12-13 0.4200265013037239 1101 14-15 0.3963953936545508 1101 16-17 0.2823957814167599 1101 18-19 0.19063579104015815 1101 20-21 0.32222052901359177 1101 22-23 0.43930918582532996 1101 24-25 0.5428679770896281 1101 26-27 0.46224247331842605 1101 28-29 0.560677657237143 1101 30-31 0.608624588031887 1101 32-33 0.5344578354773901 1101 34-35 0.49281362214018287 1101 36-37 0.5890166698095314 1101 38-39 0.5903967288941985 1101 40-41 0.5031920354240071 1101 42-43 0.4255087778600739 1101 44-45 0.3719204799957154 1101 46-47 0.26366959780563803 1101 48-49 0.28202605317508755 1101 50-51 0.2600396835743055 1101 52-53 0.11524244029132547 1101 54-55 0.048105492693288454 1101 56-57 0.001553225475319664 1101 58-59 -0.1315382431356653 1101 60-61 -0.12820673137000682 1101 62-63 -0.14213646546670589 1101 64-65 -0.25038508012675464 1101 66-67 -0.30980509222821695 1101 68-69 -0.2894367186198039 1101 70-71 -0.2948535206765115 1101 72-73 -0.3227414743456052 1101 74-75 -0.342323784897701 1101 76-77 -0.3257385721804198 1101 78-79 -0.42196585498344064 1101 80-81 -0.4575752063369549 1101 82-83 -0.507762714207761 1101 84-85 -0.47772116480401294 1101 86-87 -0.4719918457605523 1101 88-89 -0.43642822817893645 1101 90-91 -0.503621682269749 1101 92-93 -0.5134639706781243 1101 94-95 -0.5610654639135362 1101 96-97 -0.5948885206769461 1101 98-99 -0.5966363846798473 1101 100-101 -0.4000229671121289 1102 1 -0.12110466696278621 1102 2 -0.04446692728891932 1102 3 0.05659544996145982 1102 4 -0.0689544075783175 1102 5 -0.00454611252057191 1102 6 -0.021361711515659465 1102 7 0.0698074443977248 1102 8 0.07872224675475081 1102 9 0.04202639531693109 1102 10-11 0.24274778384305762 1102 12-13 0.3559560960920152 1102 14-15 0.24700777060596124 1102 16-17 0.16057538262801785 1102 18-19 0.15619477142623595 1102 20-21 0.2618040156320518 1102 22-23 0.30663104611776504 1102 24-25 0.4162376323303789 1102 26-27 0.3789455913629709 1102 28-29 0.40773219054538146 1102 30-31 0.4513672697853792 1102 32-33 0.4237229219537255 1102 34-35 0.3726379860640634 1102 36-37 0.4262628515787412 1102 38-39 0.3722447435968519 1102 40-41 0.2686992030581692 1102 42-43 0.1325116228419887 1102 44-45 0.11203323869032289 1102 46-47 0.16825497460022376 1102 48-49 0.09114383621444233 1102 50-51 0.02619460281530195 1102 52-53 -0.06262361797565319 1102 54-55 -0.16730857973138313 1102 56-57 -0.2558003260890729 1102 58-59 -0.348308248185468 1102 60-61 -0.31107444140124585 1102 62-63 -0.3484657954402621 1102 64-65 -0.4464438653543823 1102 66-67 -0.45928331291764124 1102 68-69 -0.42516378720021564 1102 70-71 -0.48244538368122747 1102 72-73 -0.46455483460974634 1102 74-75 -0.5419925629378284 1102 76-77 -0.6324873473484018 1102 78-79 -0.6540858600735788 1102 80-81 -0.6115636728012994 1102 82-83 -0.6503676245657495 1102 84-85 -0.6550612708377557 1102 86-87 -0.7358662198371988 1102 88-89 -0.6932218830755978 1102 90-91 -0.7128659938321853 1102 92-93 -0.6995107243531429 1102 94-95 -0.7232296489412171 1102 96-97 -0.7829837043057353 1102 98-99 -0.7800821332513834 1102 100-101 -0.6962938023901941 1103 1 -0.06715274447708452 1103 2 -0.01892219219000424 1103 3 0.058707302847025744 1103 4 0.009162314004292682 1103 5 0.038609781429428836 1103 6 0.0419091760257686 1103 7 0.11601944882521309 1103 8 0.18139468335630937 1103 9 0.2401491469249919 1103 10-11 0.2950495597239211 1103 12-13 0.3775627457991533 1103 14-15 0.26462426664294014 1103 16-17 0.2512476787069957 1103 18-19 0.26026658741167097 1103 20-21 0.33667961162935 1103 22-23 0.3423191246011825 1103 24-25 0.4347232014837843 1103 26-27 0.4110120586551176 1103 28-29 0.4858124701001927 1103 30-31 0.49296716495599924 1103 32-33 0.4336117141396585 1103 34-35 0.42942214055830874 1103 36-37 0.4838990370814997 1103 38-39 0.34405922926253396 1103 40-41 0.2567653256054996 1103 42-43 0.16826462292483058 1103 44-45 0.14026762684807892 1103 46-47 0.12579690384548314 1103 48-49 0.06538527907827785 1103 50-51 0.04588825626839821 1103 52-53 -0.031693252484913614 1103 54-55 -0.10488883807869342 1103 56-57 -0.1922359952919095 1103 58-59 -0.2191225194716253 1103 60-61 -0.1866121704023982 1103 62-63 -0.21326068165825518 1103 64-65 -0.3811686445529219 1103 66-67 -0.36494770583539093 1103 68-69 -0.36024576140250275 1103 70-71 -0.47999009763533707 1103 72-73 -0.47561157117811703 1103 74-75 -0.5202244650986394 1103 76-77 -0.43235376770151035 1103 78-79 -0.5807094288048802 1103 80-81 -0.6092803691222457 1103 82-83 -0.6699399778835335 1103 84-85 -0.6434630585537064 1103 86-87 -0.6576851338599354 1103 88-89 -0.6062588191952578 1103 90-91 -0.672305629353982 1103 92-93 -0.697240707779045 1103 94-95 -0.7558671374534001 1103 96-97 -0.743709505788928 1103 98-99 -0.8251986873087027 1103 100-101 -0.7521821641276496 1104 1 -0.2525872470384911 1104 2 -0.20520231520832866 1104 3 -0.06159433233566247 1104 4 -0.20456606788847154 1104 5 -0.11974869499874075 1104 6 -0.05572462363392816 1104 7 0.012298843601428189 1104 8 0.07734969517030521 1104 9 -0.03850178442632313 1104 10-11 0.030171652166750107 1104 12-13 0.10519581216348683 1104 14-15 0.03461690441791632 1104 16-17 0.015667199702690482 1104 18-19 0.016656968232339864 1104 20-21 0.08569222371386331 1104 22-23 0.14402143693781966 1104 24-25 0.19562003909472025 1104 26-27 0.20419840784826704 1104 28-29 0.14895687218788822 1104 30-31 0.19768470477080768 1104 32-33 0.12982801558098345 1104 34-35 -0.043949691340806396 1104 36-37 0.06412687431968322 1104 38-39 0.06349070134419321 1104 40-41 -0.05089360483955119 1104 42-43 -0.19010956018212255 1104 44-45 -0.1771806453104503 1104 46-47 -0.2194771559139994 1104 48-49 -0.24522184005616054 1104 50-51 -0.31316385626461596 1104 52-53 -0.44829268643412234 1104 54-55 -0.46079715498053986 1104 56-57 -0.522882042422971 1104 58-59 -0.4703201461433366 1104 60-61 -0.5205972124258906 1104 62-63 -0.5686005034989279 1104 64-65 -0.5794210813317591 1104 66-67 -0.6464604450456122 1104 68-69 -0.6639938786631774 1104 70-71 -0.8246731171586639 1104 72-73 -0.8147087219066833 1104 74-75 -0.9094750016845623 1104 76-77 -0.7479505882187745 1104 78-79 -0.7945109838372417 1104 80-81 -0.8154745151510383 1104 82-83 -0.9784084421622268 1104 84-85 -1.1012950763076468 1104 86-87 -1.0539700485975025 1104 88-89 -0.9911075551082185 1104 90-91 -1.0943227831850244 1104 92-93 -1.1580693311131292 1104 94-95 -1.1810487619457923 1104 96-97 -1.231904454139631 1104 98-99 -1.3146956977823692 1104 100-101 -1.1840370895270524 1105 1 0.1579328026586424 1105 2 0.018961253172939507 1105 3 0.10536186768506184 1105 4 -0.08206020394644042 1105 5 0.152327818226631 1105 6 0.12630612032103983 1105 7 0.08413119666916913 1105 8 0.05288360656049207 1105 9 0.04553674079861736 1105 10-11 0.05195341926709318 1105 12-13 0.11771524626706054 1105 14-15 0.023326921480830265 1105 16-17 0.13630047149963076 1105 18-19 0.0811241180336495 1105 20-21 0.05972196485085135 1105 22-23 0.046865555519865154 1105 24-25 0.1597460086071152 1105 26-27 0.0777534817121861 1105 28-29 0.08036063016847805 1105 30-31 0.09077182776028536 1105 32-33 0.032258871448767934 1105 34-35 -0.045814918534667015 1105 36-37 -0.06575969572777751 1105 38-39 -0.02726798743312031 1105 40-41 0.03279554570870857 1105 42-43 -0.1297741763713134 1105 44-45 -0.13825502242482202 1105 46-47 -0.2605288563299055 1105 48-49 -0.2996008760137272 1105 50-51 -0.1900631388753098 1105 52-53 -0.29607922212455406 1105 54-55 -0.41974636717586833 1105 56-57 -0.5748359909719127 1105 58-59 -0.6010340639146108 1105 60-61 -0.6002546639132333 1105 62-63 -0.6073024578673412 1105 64-65 -0.6924197762199711 1105 66-67 -0.6871424790997551 1105 68-69 -0.7202523697283567 1105 70-71 -0.7410681043256488 1105 72-73 -0.8043190010757435 1105 74-75 -0.9239964612011775 1105 76-77 -0.875953568578133 1105 78-79 -0.8796557146539392 1105 80-81 -0.9486172232851686 1105 82-83 -1.0563880321590382 1105 84-85 -1.0357392726655945 1105 86-87 -1.1369990283583782 1105 88-89 -1.1094356990406986 1105 90-91 -1.144897726794678 1105 92-93 -1.1749134660444653 1105 94-95 -1.169391145806152 1105 96-97 -1.254646861663609 1105 98-99 -1.2825495970364358 1105 100-101 -1.1880737286607683 1106 1 -0.042292922906849384 1106 2 -0.15234534076613926 1106 3 0.007610400135575901 1106 4 -0.04711904148250312 1106 5 -0.055537377447400615 1106 6 -0.03573264243033236 1106 7 -0.046835105934249555 1106 8 -0.09521292309350571 1106 9 -0.2591430134901529 1106 10-11 -0.2681945732082056 1106 12-13 -0.1655632171257082 1106 14-15 -0.2765853106866061 1106 16-17 -0.1425632162603634 1106 18-19 -0.11121328412438203 1106 20-21 -0.2643566114830662 1106 22-23 -0.241113901722116 1106 24-25 -0.12141670623768164 1106 26-27 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-1.1741114817951939 2308 22-23 -1.2032068134463785 2308 24-25 -1.5298382104151074 2308 26-27 -1.0173954338456355 2308 28-29 -0.6794423734167196 2308 30-31 -1.0752405789476853 2308 32-33 -0.9341396834577509 2308 34-35 -1.6636911338290403 2308 36-37 -1.7533191747285386 2308 38-39 -0.8179270406265005 2308 40-41 -2.387137477244508 2308 42-43 -1.171924294860986 2308 44-45 -1.4603576526226618 2308 46-47 -1.2606479311233088 2308 48-49 -1.4418031084096512 2308 50-51 -2.1802116215791685 2308 52-53 -2.2556336255711713 2308 54-55 -1.727113823539014 2308 56-57 -1.5456057144915292 2308 58-59 -2.3503804053316912 2308 60-61 -2.0295422987337304 2308 62-63 -1.8685706747620472 2308 64-65 -1.8756058962474995 2308 66-67 -2.464868253752819 2308 68-69 -2.917810382424044 2308 70-71 -3.1204323805098895 2308 72-73 -2.965504589963814 2308 74-75 -2.7868140616113344 2308 76-77 -3.2736871180466736 2308 78-79 -2.9761906575969483 2308 80-81 -3.2515959188302617 2308 82-83 -3.3387352064839497 2308 84-85 -3.607613008521472 2308 86-87 -3.9398709498229145 2308 88-89 -4.188299609682726 2308 90-91 -4.546152383317974 2308 92-93 -4.212122212313275 2308 94-95 -4.624584401178726 2308 96-97 -5.033747363915992 2308 98-99 -5.034717651973441 2308 100-101 -4.7809021478654685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 485.0 11 4200.0 12 14244.0 13 29133.0 14 47440.0 15 68109.0 16 95670.0 17 145225.0 18 243051.0 19 473295.0 20 510720.0 21 455700.0 22 421770.0 23 402787.0 24 407438.0 25 427583.0 26 467652.0 27 500504.0 28 539980.0 29 581752.0 30 621036.0 31 684428.0 32 797993.0 33 979384.0 34 1300451.0 35 1957771.0 36 3089294.0 37 4482029.0 38 3433256.0 39 411740.0 40 79.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.41900751718579 35.20603917860837 19.92817981298965 18.446773491216188 2 19.912108067905834 24.075923398468827 39.861305756388795 16.150662777236544 3 19.589206704257258 32.62996985445831 21.344790554899888 26.43603288638455 4 28.222497205033676 21.749127179635064 31.54260101697866 18.485774598352595 5 19.826154747679112 30.414459450673515 20.77670607380576 28.98267972784161 6 19.914222994276308 21.638337249125865 30.347332789640436 28.100106966957387 7 28.218737806856108 21.688561452512783 20.803831381964095 29.288869358667014 8 38.05716760414274 21.579683008562867 20.89381959177937 19.469329795515016 9 19.450600617897567 31.509978595588866 21.16293655534525 27.87648423116832 10-11 33.89948089789178 21.453035368604542 21.063469321827455 23.584014411676225 12-13 28.601571267381708 21.44213862371781 21.284559655800184 28.671730453100302 14-15 28.427615394663487 21.428103395910572 21.394173449900954 28.75010775952499 16-17 28.446564265759868 25.605999323475707 21.33837374021086 24.609062670553566 18-19 24.205177030685995 21.50023053334653 25.732740975902107 28.56185146006537 20-21 23.914638011491128 30.64292447780179 21.947853968530065 23.494583542177015 22-23 18.676560877117183 25.36265901258284 31.5241007091488 24.43667940115118 24-25 18.7121776782279 21.318432384362904 35.79393996881098 24.175449968598215 26-27 18.716320645216143 21.2989360691241 41.071666674719495 18.913076610940262 28-29 18.703569948862135 21.179888083753262 41.21516884590283 18.90137312148177 30-31 18.73998582694119 21.139786878561807 41.19196102747895 18.928266267018053 32-33 18.75394105360011 21.153636590321543 41.149855382909664 18.942566973168688 34-35 18.767291818656943 21.177521857460633 41.18186281201882 18.873323511863603 36-37 18.788020053619036 21.175194393856707 41.06454317043679 18.972242382087465 38-39 19.061798409275248 21.314475354506293 40.30606356634744 19.31766266987102 40-41 20.18853971395632 22.326437414153858 36.9092904837875 20.57573238810232 42-43 20.334673344279214 22.626600878364577 36.914180350764745 20.12454542659146 44-45 20.51957968244765 23.250965622442365 36.17569009209704 20.053764603012937 46-47 20.232907957669127 23.83549941711024 35.986832052939654 19.944760572280977 48-49 20.20467063505712 24.05488586061647 35.60106433782318 20.139379166503225 50-51 20.270470502875266 24.259140421106373 35.2786752801464 20.19171379587196 52-53 20.322395189998137 24.307128822300452 35.107074393407856 20.263401594293555 54-55 20.5885824008813 24.198760131648463 34.7449204515254 20.46773701594483 56-57 20.7646140612431 24.133116208904568 34.53126932748567 20.571000402366664 58-59 20.8808674982942 24.253827031625356 34.2148356035684 20.65046986651204 60-61 21.026458527692682 24.361045636223384 33.7463550966428 20.866140739441136 62-63 21.242235969562017 24.56745139751965 33.143074230654854 21.047238402263478 64-65 21.442632389092882 24.761028154289768 32.608025903991155 21.18831355262619 66-67 21.64098407437831 24.879361657841 32.024182386041254 21.455471881739438 68-69 21.751896319166285 25.038675759950763 31.607135659167234 21.602292261715718 70-71 21.82992051832032 25.213973772658495 31.239021086613366 21.71708462240782 72-73 21.973260180003624 25.309442623836016 30.847298817129253 21.8699983790311 74-75 22.174261529005502 25.363917006516377 30.538625970078193 21.923195494399927 76-77 22.26110296818775 25.501092841490873 30.170906447453266 22.06689774286811 78-79 22.399823112381558 25.42732387687627 29.924591543443448 22.248261467298732 80-81 22.48586768650565 25.500973159184824 29.664732625947128 22.3484265283624 82-83 22.624407846208207 25.61867895026026 29.39511428048709 22.361798923044446 84-85 22.697011831122545 25.62055152511162 29.231573906576973 22.45086273718886 86-87 22.85150483058766 25.781090116697058 28.883413078274977 22.483991974440308 88-89 22.9126296425142 25.872870316119545 28.67077175864894 22.543728282717314 90-91 23.10799005907431 25.83564778112027 28.33151318115259 22.72484897865283 92-93 23.119711098232624 25.905880766797868 28.189559138885095 22.784848996084413 94-95 23.17558509504311 26.03364919007054 28.010689568505253 22.7800761463811 96-97 23.250144157321255 26.003170719354042 27.843357732882684 22.903327390442023 98-99 23.375617016287066 26.04036277814408 27.708651355247255 22.875368850321603 100-101 23.78437774916935 25.815380596771853 27.35596548597182 23.044276168086977 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9738.0 1 5636.5 2 1310.0 3 927.0 4 723.5 5 659.0 6 672.0 7 724.5 8 761.5 9 760.5 10 836.5 11 984.5 12 1482.5 13 2760.5 14 4516.5 15 8722.0 16 183608.0 17 229370.0 18 99037.5 19 93493.5 20 93533.5 21 97738.0 22 104935.0 23 114197.5 24 125906.0 25 140608.0 26 158641.5 27 180508.0 28 206326.0 29 237567.5 30 277593.5 31 321958.5 32 367000.0 33 418266.5 34 466922.5 35 513511.5 36 566381.0 37 614212.0 38 650083.5 39 682226.5 40 714985.5 41 742995.5 42 771272.0 43 808178.0 44 845052.0 45 867745.5 46 869754.0 47 865104.5 48 861307.5 49 850452.5 50 834478.0 51 819337.0 52 802284.5 53 780340.0 54 740394.0 55 683883.0 56 623779.0 57 554609.0 58 487649.5 59 421740.0 60 350417.5 61 289244.0 62 238019.5 63 188343.5 64 146811.5 65 113136.0 66 83915.0 67 61296.5 68 45873.0 69 35478.5 70 28032.0 71 22433.0 72 18244.0 73 14058.0 74 10103.0 75 7811.0 76 5929.5 77 3874.5 78 2434.5 79 1517.0 80 827.5 81 460.5 82 262.5 83 155.5 84 87.0 85 44.5 86 21.5 87 7.0 88 5.5 89 4.0 90 2.5 91 5.0 92 5.0 93 1.5 94 2.0 95 3.5 96 2.0 97 0.5 98 3.5 99 4.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.178747049804863E-4 18-19 6.357494099609727E-6 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.716523406894626E-4 30-31 6.018427747630541E-4 32-33 0.005124140244285439 34-35 0.0013265971021185628 36-37 6.632985510592814E-4 38-39 2.161547993867307E-4 40-41 0.0016656634540977483 42-43 0.0010193182206374263 44-45 0.0025938575926407683 46-47 0.00231836618165768 48-49 6.357494099609727E-6 50-51 8.900491739453617E-5 52-53 4.89527045669949E-4 54-55 1.483415289908936E-5 56-57 2.1403563468686077E-4 58-59 0.001807647488989032 60-61 5.361486690670869E-4 62-63 2.6065725808399875E-4 64-65 0.0 66-67 0.0014198403489128388 68-69 0.00533605671427243 70-71 0.006495239805101271 72-73 0.0012227580318249374 74-75 0.0034224509902899027 76-77 0.0047426905983088555 78-79 0.007720117001626078 80-81 0.005071161126788692 82-83 0.002388298616753387 84-85 0.0015745393720033422 86-87 0.001413482854813229 88-89 3.8144964597658354E-4 90-91 8.900491739453617E-5 92-93 6.844901980579806E-4 94-95 7.289926567552486E-4 96-97 7.925675977513459E-4 98-99 4.768120574707295E-4 100-101 6.082002688626638E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.3594202E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.743666141991405 #Duplication Level Percentage of deduplicated Percentage of total 1 75.71590265008501 37.663865830653506 2 13.061778027471327 12.994814508386654 3 4.717883275121729 7.040544316036207 4 2.2193014359729895 4.4158475879793 5 1.24687774403646 3.1012135109614536 6 0.7576496544136236 2.2612962877047873 7 0.4906255807654156 1.708386056322083 8 0.3327473280407457 1.324165759655884 9 0.2372776475915884 1.0622754076277756 >10 1.0761890884060907 9.344813478700866 >50 0.07708484254875801 2.7290218256201944 >100 0.06499484758003858 5.3478606311285715 >500 8.049525502074546E-4 0.27180005932848883 >1k 6.763657644697448E-4 0.7271162728567411 >5k 8.73906218822636E-5 0.3094958905407885 >10k+ 1.1916902983935249E-4 9.697482576496853 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 2046382 8.673240993698366 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 119182 0.5051325745197909 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67260 0.2850700354265001 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34620 0.14673096381899248 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 23962 0.10155884907656551 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016275184895000899 0.0 0.0 0.0 0.0 2 0.0016444718070990493 0.0 0.0 4.238329399739817E-6 0.0 3 0.0016529484658985287 0.0 0.0 8.476658799479635E-6 4.238329399739817E-6 4 0.0016571867952982686 0.0 0.0 1.2714988199219452E-5 1.2714988199219452E-5 5 0.0016826167716967075 4.238329399739817E-6 0.0 1.695331759895927E-5 1.695331759895927E-5 6 0.0017038084186954067 4.238329399739817E-6 0.0 1.695331759895927E-5 1.695331759895927E-5 7 0.001737715053893325 4.238329399739817E-6 0.0 1.695331759895927E-5 2.1191646998699087E-5 8 0.0017461917126928047 4.238329399739817E-6 0.0 2.1191646998699087E-5 2.5429976398438904E-5 9 0.0018140049830886419 4.238329399739817E-6 0.0 2.1191646998699087E-5 2.5429976398438904E-5 10-11 0.0018945332416836986 4.238329399739817E-6 0.0 3.602579989778845E-5 2.966830579817872E-5 12-13 0.0020492322647742015 4.238329399739817E-6 0.0 4.2383293997398174E-5 3.602579989778845E-5 14-15 0.002186977970265746 4.238329399739817E-6 0.0 4.2383293997398174E-5 4.4502458697268084E-5 16-17 0.00232260451105742 6.357494099609726E-6 0.0 4.87407880970079E-5 5.933661159635744E-5 18-19 0.0024476352283497446 8.476658799479635E-6 0.0 6.145577629622735E-5 6.357494099609726E-5 20-21 0.002606572580839988 8.476658799479635E-6 0.0 6.357494099609726E-5 6.569410569596717E-5 22-23 0.0027718674274298406 8.476658799479635E-6 0.0 6.781327039583708E-5 7.20515997955769E-5 24-25 0.0029753072386173515 8.476658799479635E-6 0.0 6.781327039583708E-5 8.476658799479635E-5 26-27 0.0032190111791023913 8.476658799479635E-6 0.0 6.781327039583708E-5 8.476658799479635E-5 28-29 0.003411855166790553 8.476658799479635E-6 0.0 7.840909389518662E-5 9.112408209440608E-5 30-31 0.0036110566485783244 8.476658799479635E-6 0.0 8.476658799479635E-5 9.74815761940158E-5 32-33 0.0038441647655640147 8.476658799479635E-6 0.0 8.688575269466626E-5 1.1443489379297508E-4 34-35 0.004284951023136955 8.476658799479635E-6 0.0 8.900491739453617E-5 1.250307172923246E-4 36-37 0.005673003901551746 8.476658799479635E-6 0.0 8.900491739453617E-5 1.3138821139193434E-4 38-39 0.0060290235711298905 8.476658799479635E-6 0.0 9.324324679427599E-5 1.3986487019141398E-4 40-41 0.006416830711206084 8.476658799479635E-6 0.0 1.0171990559375562E-4 1.5257985839063344E-4 42-43 0.0069550985449730405 8.476658799479635E-6 0.0 1.0383907029362553E-4 1.6317568188998295E-4 44-45 0.007686210366428158 8.476658799479635E-6 0.0 1.0595823499349544E-4 1.6529484658985287E-4 46-47 0.008466062975980286 8.476658799479635E-6 0.0 1.1019656439323526E-4 1.695331759895927E-4 48-49 0.009442997902620313 8.476658799479635E-6 0.0 1.1443489379297507E-4 1.7800983478907234E-4 50-51 0.010568274358251235 8.476658799479635E-6 0.0 1.207923878925848E-4 1.9284398768816167E-4 52-53 0.01205168964816017 8.476658799479635E-6 0.0 1.2714988199219451E-4 2.0343981118751123E-4 54-55 0.013848741313649854 8.476658799479635E-6 0.0 1.2926904669206444E-4 2.140356346868608E-4 56-57 0.015868305272625876 8.476658799479635E-6 0.0 1.3350737609180423E-4 2.2675062288608023E-4 58-59 0.017976874148996437 1.0595823499349543E-5 0.0 1.441031995911538E-4 2.3310811698568995E-4 60-61 0.020528348447639806 1.2714988199219452E-5 0.0 1.441031995911538E-4 2.415847757851696E-4 62-63 0.023779147097240244 1.2714988199219452E-5 0.0 1.5257985839063344E-4 2.479422698847793E-4 64-65 0.02759364355700608 1.2714988199219452E-5 0.0 1.5681818779037323E-4 2.5641892868425895E-4 66-67 0.03187223708604343 1.2714988199219452E-5 0.0 1.7377150538933252E-4 2.670147521836085E-4 68-69 0.0368882999306355 1.2714988199219452E-5 0.0 1.8436732888868208E-4 2.712530815833483E-4 70-71 0.04803298708725135 1.483415289908936E-5 0.0 1.8648649358855198E-4 2.818489050826978E-4 72-73 0.05994057353582037 1.695331759895927E-5 0.0 1.949631523880316E-4 2.903255638821775E-4 74-75 0.06905933923936058 1.695331759895927E-5 0.0 1.992014817877714E-4 3.0092138738152703E-4 76-77 0.0807698433708417 1.695331759895927E-5 0.0 2.0343981118751123E-4 3.178747049804863E-4 78-79 0.09423501587381511 1.695331759895927E-5 0.0 2.0979730528712095E-4 3.221130343802261E-4 80-81 0.11045086415721964 1.695331759895927E-5 2.1191646998699087E-6 2.161547993867307E-4 3.2635136377996596E-4 82-83 0.1286099864704049 1.695331759895927E-5 4.238329399739817E-6 2.161547993867307E-4 3.284705284798358E-4 84-85 0.15033142464407145 1.695331759895927E-5 4.238329399739817E-6 2.161547993867307E-4 3.3058969317970575E-4 86-87 0.17534180643193611 1.695331759895927E-5 4.238329399739817E-6 2.161547993867307E-4 3.4754301077866503E-4 88-89 0.20528348447639805 2.1191646998699087E-5 4.238329399739817E-6 2.2039312878647052E-4 3.5813883427801454E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 196915 0.0 94.1561 1 TATCTCG 250110 0.0 92.5419 2 TCGACGG 201890 0.0 91.90883 2 ATATCTC 252720 0.0 91.65946 1 CGACGGC 203910 0.0 90.8283 3 ACGGCGC 206120 0.0 89.877495 5 GACGGCG 206395 0.0 89.785355 4 CGGCGCG 207655 0.0 89.20396 6 ATCTCGA 259320 0.0 89.095825 3 TCTCGAG 260830 0.0 87.71688 4 CTCGAGG 265665 0.0 86.18125 5 TCGAGGG 266175 0.0 85.94297 6 CGAGGGC 268275 0.0 85.20648 7 AGGGCGC 271195 0.0 84.310074 9 GAGGGCG 273485 0.0 83.74479 8 CTCGACG 4135 0.0 54.79185 5 GGGCGCG 273515 0.0 41.910435 10-11 CCATATT 222405 0.0 41.684536 18-19 TCCATAT 220765 0.0 41.64458 18-19 ATCCATA 225965 0.0 40.827244 16-17 >>END_MODULE