FastQCFastQC Report
Tue 31 May 2016
SRR1051281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23594202
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1125850.4771723154697073No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT955950.40516309896812785No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409920.1737375987541346No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308580.13078636861717127No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288590.1223139481470914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG137000.085.1323851
TCGACGG154350.075.644092
CGACGGC183200.063.8593
ATCTCGA206900.058.46211
ACGGCGC207950.056.4184575
GACGGCG209100.056.108174
CGGCGCG229150.051.1159446
TCTCGAG253600.047.819092
CTCGAGG310150.039.1140063
TCGAGGG311700.038.828074
TATCTCG64300.038.1187362
CGAGGGC340500.035.7113275
AGGGCGC373400.032.4630667
GGGCGCG373550.032.386458
GAGGGCG403100.030.141926
CGCGCCG597400.028.003189
GGCGCGC616750.027.1708097
GCGCGCC624050.026.8681958
ATATCTC118300.021.3416291
CCATATT348050.019.43352318-19
Produced by FastQC (version 0.11.5)