##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051281_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23594202 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15037808865076 33.0 31.0 34.0 31.0 34.0 2 32.430913577835774 34.0 31.0 34.0 31.0 34.0 3 32.45037886850337 34.0 31.0 34.0 31.0 34.0 4 36.12903937162189 37.0 35.0 37.0 35.0 37.0 5 36.09375481315283 37.0 35.0 37.0 35.0 37.0 6 36.079746922570216 37.0 35.0 37.0 35.0 37.0 7 36.07741719766577 37.0 35.0 37.0 35.0 37.0 8 36.07177305678743 37.0 35.0 37.0 35.0 37.0 9 37.78596953607501 39.0 38.0 39.0 35.0 39.0 10-11 37.77919126063259 39.0 38.0 39.0 35.0 39.0 12-13 37.75718875764478 39.0 38.0 39.0 35.0 39.0 14-15 39.14894847895258 40.0 39.0 41.0 36.0 41.0 16-17 39.11794808741571 40.0 38.5 41.0 36.0 41.0 18-19 39.09818850834625 40.0 38.5 41.0 36.0 41.0 20-21 39.09024047518115 40.0 39.0 41.0 36.0 41.0 22-23 39.06459269103486 40.0 39.0 41.0 36.0 41.0 24-25 39.00828112347263 40.0 38.0 41.0 35.5 41.0 26-27 38.86295480559164 40.0 38.0 41.0 35.0 41.0 28-29 38.692658348860455 40.0 38.0 41.0 35.0 41.0 30-31 38.49238361611043 40.0 38.0 41.0 34.5 41.0 32-33 38.54879514467156 40.0 38.0 41.0 35.0 41.0 34-35 38.54388525197843 40.0 38.0 41.0 35.0 41.0 36-37 38.52340373707066 40.0 38.0 41.0 35.0 41.0 38-39 38.45988510228064 40.0 38.0 41.0 34.5 41.0 40-41 38.32778934417871 40.0 38.0 41.0 34.0 41.0 42-43 38.23603459866962 40.0 38.0 41.0 34.0 41.0 44-45 38.134546360160854 40.0 37.5 41.0 33.5 41.0 46-47 37.99967180496293 40.0 37.0 41.0 33.0 41.0 48-49 37.91853731692218 40.0 37.0 41.0 33.0 41.0 50-51 37.92385699249333 40.0 37.0 41.0 33.0 41.0 52-53 37.78860478095424 40.0 37.0 41.0 33.0 41.0 54-55 37.674394815302506 40.0 36.5 41.0 33.0 41.0 56-57 37.4897070687112 39.0 36.0 41.0 33.0 41.0 58-59 37.24208260995646 39.0 35.5 41.0 32.0 41.0 60-61 37.01506452729361 39.0 35.0 40.5 32.0 41.0 62-63 36.648784879437756 38.5 35.0 40.0 31.5 41.0 64-65 36.279828175583134 37.5 35.0 40.0 31.0 41.0 66-67 35.87608724804509 37.0 35.0 40.0 31.0 41.0 68-69 35.47418990902935 36.5 34.5 39.0 30.5 41.0 70-71 35.05026529399045 36.0 34.0 39.0 30.0 40.5 72-73 34.67251011498503 35.5 34.0 38.5 30.0 40.0 74-75 34.29383475228363 35.0 34.0 37.0 29.5 39.0 76-77 33.70303689440313 35.0 33.0 37.0 29.0 39.0 78-79 33.483559054042175 35.0 33.0 36.0 29.0 39.0 80-81 33.15054281132288 35.0 33.0 36.0 29.0 37.0 82-83 32.81067253302315 35.0 33.0 35.5 28.0 37.0 84-85 32.57666061348462 35.0 33.0 35.0 27.5 36.5 86-87 32.31246485895136 35.0 33.0 35.0 27.0 36.0 88-89 32.0792249087297 35.0 33.0 35.0 27.0 36.0 90-91 31.866444879127506 35.0 32.0 35.0 26.0 35.5 92-93 31.633249600897713 34.0 32.0 35.0 25.5 35.0 94-95 31.44010886657663 34.0 32.0 35.0 25.0 35.0 96-97 31.250008582617035 34.0 32.0 35.0 25.0 35.0 98-99 30.92716897142781 34.0 32.0 35.0 24.0 35.0 100-101 29.395578922313202 33.0 29.5 34.5 12.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23216642667904352 1101 2 0.18514872433283358 1101 3 0.0699135802652151 1101 4 0.11871081695307595 1101 5 0.21785384250357254 1101 6 0.25198023666484204 1101 7 0.29062128206920335 1101 8 0.2842125953770278 1101 9 0.38449682195612667 1101 10-11 0.39972763314565185 1101 12-13 0.38099377861015427 1101 14-15 0.4774826283253688 1101 16-17 0.43392248947824186 1101 18-19 0.44906188012583215 1101 20-21 0.4310033631873651 1101 22-23 0.4449502406197752 1101 24-25 0.46586225860027497 1101 26-27 0.49501325371928573 1101 28-29 0.502998117135327 1101 30-31 0.5055969541664496 1101 32-33 0.4842301605383099 1101 34-35 0.4753759064671357 1101 36-37 0.42039285157365214 1101 38-39 0.36436668591525034 1101 40-41 0.3209771780184312 1101 42-43 0.28334126268842397 1101 44-45 0.297542247191835 1101 46-47 0.2536428390175729 1101 48-49 0.30145721193876795 1101 50-51 0.3148371999007935 1101 52-53 0.343412689522836 1101 54-55 0.41938827690182734 1101 56-57 0.39919891489520154 1101 58-59 0.39545726622014143 1101 60-61 0.36784790524594513 1101 62-63 0.3556845424845889 1101 64-65 0.38694216009680815 1101 66-67 0.3530386994447525 1101 68-69 0.3040531201566168 1101 70-71 0.30335552344173067 1101 72-73 0.28328566831773827 1101 74-75 0.1907059098401902 1101 76-77 0.13571499776425355 1101 78-79 0.16796300047604262 1101 80-81 0.1292509549209342 1101 82-83 0.1908398763248087 1101 84-85 0.09534156357339185 1101 86-87 0.010558210728952133 1101 88-89 -0.02809660605673514 1101 90-91 -0.06946623677314534 1101 92-93 -0.12089695842721682 1101 94-95 -0.1826820490679637 1101 96-97 -0.3800036118103627 1101 98-99 -0.3725195635494245 1101 100-101 -0.2117663537566692 1102 1 0.23704104064645293 1102 2 0.20647343642014704 1102 3 0.10229635626092204 1102 4 0.14981291867665192 1102 5 0.21453182830761364 1102 6 0.23394998543583512 1102 7 0.2445430618533635 1102 8 0.22905015437754628 1102 9 0.3096836810092114 1102 10-11 0.3274190347009025 1102 12-13 0.3221700667628724 1102 14-15 0.4035011573055982 1102 16-17 0.38977709788372294 1102 18-19 0.40420118828637186 1102 20-21 0.36634519216929107 1102 22-23 0.36073854846836895 1102 24-25 0.38860340857600306 1102 26-27 0.41549477706847426 1102 28-29 0.4276328541833223 1102 30-31 0.4279991749104042 1102 32-33 0.3953194895184211 1102 34-35 0.40904701181032976 1102 36-37 0.3550530295010077 1102 38-39 0.30706253301281805 1102 40-41 0.2880301267446086 1102 42-43 0.2390506955954379 1102 44-45 0.2572101944489589 1102 46-47 0.20828179329569707 1102 48-49 0.2191137957089424 1102 50-51 0.23605437805984053 1102 52-53 0.2790602817812555 1102 54-55 0.3665844860973948 1102 56-57 0.30119931586949633 1102 58-59 0.27233435105256376 1102 60-61 0.26065350414803135 1102 62-63 0.269890647199027 1102 64-65 0.3096341484996543 1102 66-67 0.2909287253497155 1102 68-69 0.20194723009520033 1102 70-71 0.19164084363131906 1102 72-73 0.21098813631396496 1102 74-75 0.18504068632958592 1102 76-77 0.07071703025340526 1102 78-79 0.05779390161131914 1102 80-81 0.05219592880824564 1102 82-83 0.08469697600438053 1102 84-85 0.006522852607432128 1102 86-87 -0.04900641252490345 1102 88-89 -0.11496278361187251 1102 90-91 -0.17339641078572043 1102 92-93 -0.21971854092979015 1102 94-95 -0.3460531730097003 1102 96-97 -0.4943671962849159 1102 98-99 -0.5430902854815152 1102 100-101 -0.28464030120287376 1103 1 0.17826829838458735 1103 2 0.1666019469857929 1103 3 0.1390550764079066 1103 4 0.14373443925655494 1103 5 0.19429494895864963 1103 6 0.19398478363154936 1103 7 0.1915704802435485 1103 8 0.15470191675586165 1103 9 0.23879055928592408 1103 10-11 0.2565854425064842 1103 12-13 0.24966275402278626 1103 14-15 0.30915911645845995 1103 16-17 0.3246656880411436 1103 18-19 0.25583364773520145 1103 20-21 0.26153988046058174 1103 22-23 0.2712009436269227 1103 24-25 0.3050314720340239 1103 26-27 0.30092769329471736 1103 28-29 0.30409410217204424 1103 30-31 0.32603088354942855 1103 32-33 0.271108857765995 1103 34-35 0.26175257911104666 1103 36-37 0.2548934359693149 1103 38-39 0.16275715927636014 1103 40-41 0.14892474366560293 1103 42-43 0.08919054805861037 1103 44-45 0.10990170709514047 1103 46-47 0.10632715958085726 1103 48-49 0.1115307753142929 1103 50-51 0.14906194308023402 1103 52-53 0.22306527355814865 1103 54-55 0.2440949197441924 1103 56-57 0.19417942946135724 1103 58-59 0.20548385385826862 1103 60-61 0.18094916687294926 1103 62-63 0.2016761807054266 1103 64-65 0.2029410649182637 1103 66-67 0.17971240165751112 1103 68-69 0.11032867828799908 1103 70-71 0.07813594733175222 1103 72-73 0.05857505008778929 1103 74-75 -0.0030675534875754806 1103 76-77 -0.09616394971938291 1103 78-79 -0.08763218420939722 1103 80-81 -0.09056687171918298 1103 82-83 -0.09531038822560589 1103 84-85 -0.15856470976250137 1103 86-87 -0.2295330021696671 1103 88-89 -0.27704605694098916 1103 90-91 -0.3411418089862863 1103 92-93 -0.3931770983867402 1103 94-95 -0.4746633749457274 1103 96-97 -0.65420116016222 1103 98-99 -0.6615565244213464 1103 100-101 -0.5198782383020095 1104 1 -0.03141095463398358 1104 2 -0.10863554529701958 1104 3 -0.20677652041229777 1104 4 -0.12547741278256552 1104 5 -0.011531080854190634 1104 6 0.10791550603558875 1104 7 0.17303779198123692 1104 8 0.18410873140291528 1104 9 0.28133235975878534 1104 10-11 0.27738152621449075 1104 12-13 0.298399224109815 1104 14-15 0.34867762179185036 1104 16-17 0.3574964258768034 1104 18-19 0.3167035724175946 1104 20-21 0.2617581269120919 1104 22-23 0.21827279163597524 1104 24-25 0.2210041153636979 1104 26-27 0.26276118101999657 1104 28-29 0.2839711903299502 1104 30-31 0.26936139073168874 1104 32-33 0.23442987798495807 1104 34-35 0.17085314499987447 1104 36-37 0.20368254886754755 1104 38-39 0.1786302699131852 1104 40-41 0.0750213315434749 1104 42-43 0.11722838277410119 1104 44-45 0.2013604000139253 1104 46-47 0.11207234595718774 1104 48-49 0.10621088855534566 1104 50-51 0.08004247785832774 1104 52-53 0.11296463206249996 1104 54-55 0.21121488125023546 1104 56-57 0.10595983756698502 1104 58-59 0.1021973721357341 1104 60-61 0.06853767321403836 1104 62-63 0.05630904505219547 1104 64-65 0.13093934844308563 1104 66-67 0.15046020330171928 1104 68-69 -0.04340117786364317 1104 70-71 -0.04577809929440235 1104 72-73 0.004442063092618298 1104 74-75 -0.04324294056705469 1104 76-77 -0.15249163716238456 1104 78-79 -0.14992957056870893 1104 80-81 -0.2242953133793435 1104 82-83 -0.14782783214203477 1104 84-85 -0.27248657457593595 1104 86-87 -0.3663082848648358 1104 88-89 -0.3959644097217563 1104 90-91 -0.5072756116315951 1104 92-93 -0.49717989522306283 1104 94-95 -0.5011862334819597 1104 96-97 -0.7408472737203873 1104 98-99 -0.754759359630615 1104 100-101 -0.7195718497738994 1105 1 0.008670620082440905 1105 2 0.06654767175332665 1105 3 0.1337202153337813 1105 4 0.09644530539066665 1105 5 0.1442889768596416 1105 6 0.10006754927742634 1105 7 0.12970084371322343 1105 8 0.10751651107324989 1105 9 0.12611384412397086 1105 10-11 0.17260260522619575 1105 12-13 0.1955844640947717 1105 14-15 0.21140870839968784 1105 16-17 0.16948827259749777 1105 18-19 0.11179303041482314 1105 20-21 -0.012410647869032232 1105 22-23 0.019348079345235192 1105 24-25 0.09441580949562933 1105 26-27 0.09479256432792482 1105 28-29 0.09689322419388446 1105 30-31 0.07263747982324276 1105 32-33 0.10945070736504903 1105 34-35 0.1228408946107038 1105 36-37 0.03850684812115901 1105 38-39 0.007283884745085345 1105 40-41 -0.032453811656893095 1105 42-43 0.015570538660185207 1105 44-45 -0.0031879939053993667 1105 46-47 -0.044857299996024835 1105 48-49 0.023609548548840564 1105 50-51 0.03305360177647998 1105 52-53 0.07183009196067758 1105 54-55 0.11677755003920254 1105 56-57 0.08213314287375084 1105 58-59 0.05772522370328659 1105 60-61 0.02088538232165149 1105 62-63 0.017458975665171295 1105 64-65 0.08998253544166346 1105 66-67 0.06277285251103848 1105 68-69 0.053961579604674625 1105 70-71 0.04827056139725272 1105 72-73 -0.005377357629065216 1105 74-75 0.010441057392853281 1105 76-77 -0.0693357519301685 1105 78-79 -0.041820395699154744 1105 80-81 -0.047662526272432615 1105 82-83 0.008676661946367403 1105 84-85 -0.09956028922486126 1105 86-87 -0.17959791517483126 1105 88-89 -0.256255253693368 1105 90-91 -0.253216024893554 1105 92-93 -0.323419272113199 1105 94-95 -0.3409892576147371 1105 96-97 -0.5590772719327184 1105 98-99 -0.6240531932788365 1105 100-101 -0.4477760469437655 1106 1 -0.0362017279070912 1106 2 0.01181986264808188 1106 3 0.11792248210867484 1106 4 0.07165374325207097 1106 5 0.04448247089035107 1106 6 1.8225304425811828E-4 1106 7 0.04524233658336385 1106 8 0.012949831100712572 1106 9 -0.053280745627951376 1106 10-11 0.035785835702519364 1106 12-13 0.03179868511578121 1106 14-15 0.029930736507466804 1106 16-17 -0.017103604287420637 1106 18-19 -0.02509849054448665 1106 20-21 -0.00804952491854749 1106 22-23 -0.056847825937396124 1106 24-25 -0.01954872548202502 1106 26-27 -0.06445869197387566 1106 28-29 -0.06997487101085653 1106 30-31 -0.0605479254534913 1106 32-33 -0.06385367570116784 1106 34-35 -0.0876949081806373 1106 36-37 -0.1441363854204809 1106 38-39 -0.1779364027417074 1106 40-41 -0.21823138433314426 1106 42-43 -0.22279472891473517 1106 44-45 -0.2633651850177472 1106 46-47 -0.2987861444169013 1106 48-49 -0.305797778501919 1106 50-51 -0.22406454592329794 1106 52-53 -0.2705955195831393 1106 54-55 -0.20926567993963374 1106 56-57 -0.18911555698434768 1106 58-59 -0.22407498843330131 1106 60-61 -0.23365840153233108 1106 62-63 -0.19821822629682373 1106 64-65 -0.16500422847192908 1106 66-67 -0.2264859210862511 1106 68-69 -0.19304878335022124 1106 70-71 -0.20773057767241454 1106 72-73 -0.2642268511876793 1106 74-75 -0.23312822626354546 1106 76-77 -0.3530582585568922 1106 78-79 -0.36783396930864143 1106 80-81 -0.34796012460850534 1106 82-83 -0.27919076890518113 1106 84-85 -0.3366638804986337 1106 86-87 -0.4277474356322557 1106 88-89 -0.5281307087232179 1106 90-91 -0.6134368663513925 1106 92-93 -0.6840844331021607 1106 94-95 -0.679499822267509 1106 96-97 -0.8738238646300367 1106 98-99 -0.8703524580323609 1106 100-101 -0.74149904220819 1107 1 -0.1261912159669123 1107 2 -0.06911931040668406 1107 3 0.06786546263262494 1107 4 0.036112833951371215 1107 5 0.006933608070816888 1107 6 -0.04398332595117438 1107 7 -0.0627726294725548 1107 8 -0.06408038117687909 1107 9 -0.08655327301046611 1107 10-11 -0.027129113158245843 1107 12-13 -0.025694855434018393 1107 14-15 -0.06682731799895691 1107 16-17 -0.13013472308815466 1107 18-19 -0.18917179922886618 1107 20-21 -0.15751412555592026 1107 22-23 -0.1355979984897857 1107 24-25 -0.11390800695649972 1107 26-27 -0.16917909742792858 1107 28-29 -0.14730230256778754 1107 30-31 -0.19344188982164923 1107 32-33 -0.1435786572950164 1107 34-35 -0.15398696697638314 1107 36-37 -0.20910171977014613 1107 38-39 -0.2794094134313738 1107 40-41 -0.2950955164224709 1107 42-43 -0.3343362295732817 1107 44-45 -0.32219241920516595 1107 46-47 -0.29468215025030986 1107 48-49 -0.2656789561917563 1107 50-51 -0.22806954862038253 1107 52-53 -0.3089449256384498 1107 54-55 -0.34686211887421337 1107 56-57 -0.3288883879518778 1107 58-59 -0.2581748408079463 1107 60-61 -0.27793494846889644 1107 62-63 -0.2909021978015005 1107 64-65 -0.2670641920574184 1107 66-67 -0.2854255863236972 1107 68-69 -0.2519814248041996 1107 70-71 -0.19546069845856096 1107 72-73 -0.29315594920439736 1107 74-75 -0.3083410375699316 1107 76-77 -0.4611755720132962 1107 78-79 -0.3544515925169165 1107 80-81 -0.35562554056254214 1107 82-83 -0.26129313840158375 1107 84-85 -0.3147866973284721 1107 86-87 -0.32495591465576723 1107 88-89 -0.37670821558868894 1107 90-91 -0.3692511385411308 1107 92-93 -0.4617387408091105 1107 94-95 -0.4416995943501618 1107 96-97 -0.6061815954437648 1107 98-99 -0.6483410803448955 1107 100-101 -0.398174852625111 1108 1 -0.30277287543029274 1108 2 -0.1918883153674571 1108 3 -0.019441043473854336 1108 4 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-1.6439114169609397 2308 80-81 -1.3505637437184497 2308 82-83 -0.9843012271746403 2308 84-85 -1.1084705953827871 2308 86-87 -0.9273837496394961 2308 88-89 -1.2766977277780782 2308 90-91 -1.2427548617983035 2308 92-93 -1.270214652906347 2308 94-95 -1.6605043304165008 2308 96-97 -1.5432070738957222 2308 98-99 -1.30311033486959 2308 100-101 -1.6216137297067164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 50.0 12 258.0 13 1118.0 14 3652.0 15 8587.0 16 15675.0 17 24981.0 18 41788.0 19 70841.0 20 79521.0 21 80844.0 22 83704.0 23 90408.0 24 102257.0 25 120595.0 26 147101.0 27 184945.0 28 236672.0 29 299733.0 30 381519.0 31 496974.0 32 654125.0 33 893804.0 34 1290284.0 35 1999772.0 36 3363481.0 37 6031638.0 38 5951858.0 39 937569.0 40 445.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.587996117828 30.051154068832997 24.133824206268113 22.227025607070892 2 23.906311199731213 28.66039373903054 26.93974736856918 20.493547692669072 3 23.23529511490112 26.979384764052185 26.395364114909597 23.3899560061371 4 24.506461375553197 26.67834241649707 26.331998852938533 22.4831973550112 5 24.34647291737182 27.55880025101082 25.31132436689319 22.783402464724173 6 24.407153079387893 26.54506814852225 24.6188152496109 24.428963522478956 7 24.71841175217539 26.78880599564249 25.485973206468266 23.006809045713858 8 24.270437287940485 26.30112686159083 25.563585494436303 23.864850356032385 9 23.804454162086092 25.88660553130807 25.898184647228163 24.410755659377674 10-11 23.898089454349844 26.345101648277826 25.75627902143077 24.000529875941556 12-13 23.877548390914004 26.277883863162653 26.047295433005107 23.79727231291823 14-15 23.57401619262224 26.331354626869768 26.147343317650666 23.947285862857324 16-17 23.740438434832424 26.59300365403331 26.07738121424916 23.589176696885108 18-19 23.261322845332934 26.44945143726412 26.66823188171399 23.620993835688957 20-21 22.980802656517056 26.39864022525534 26.73612991869782 23.884427199529785 22-23 23.01496782980836 26.306581591528293 27.288538938507013 23.389911640156342 24-25 23.028725447039914 26.000099515974306 27.828497865704467 23.142677171281317 26-27 23.0419702264141 25.994038704932677 27.92547931902931 23.03851174962391 28-29 23.063969275163448 26.05609632400367 27.875882388393556 23.004052012439328 30-31 23.097926770314164 26.006431580097516 27.82968035960699 23.065961289981328 32-33 23.09112783880937 26.041897456961934 27.817963147342077 23.04901155688662 34-35 23.12651006378601 26.090002959201584 27.783986930348398 22.999500046664007 36-37 23.158975666988017 26.085838800566343 27.673078326616007 23.082107205829637 38-39 23.16608546455608 26.115267640753437 27.598750743932772 23.119896150757715 40-41 23.265251776686494 26.29162876540601 27.28428577495437 23.158833682953126 42-43 23.28974932061699 26.323191604445874 27.215684175290182 23.171374899646953 44-45 23.252104478888498 26.408930889037908 27.20548463558971 23.13347999648388 46-47 23.25830727396502 26.531331722937697 27.093933924953255 23.116427078144028 48-49 23.275803097727145 26.509250026765052 27.047257203273922 23.167689672233884 50-51 23.24463018499206 26.550482614330416 27.073797621975093 23.13108957870243 52-53 23.265035621887105 26.580655281327164 27.028784444585156 23.12552465220057 54-55 23.255923213677665 26.591602886166694 26.9775748296128 23.17489907054284 56-57 23.251324626278947 26.61160568176877 26.96715489678354 23.169914795168744 58-59 23.237520387423995 26.718176355360523 26.928653912516303 23.115649344699175 60-61 23.21609139397891 26.69378053133562 26.872332872287863 23.217795202397607 62-63 23.26762997255915 26.75530468789122 26.813958929697197 23.16310640985244 64-65 23.265582232560902 26.840905439142492 26.74473191295802 23.14878041533858 66-67 23.246338316450455 26.821251480900948 26.700520372248775 23.231889830399822 68-69 23.25328212144439 26.898310441249674 26.635048600596576 23.213358836709357 70-71 23.2528533706692 27.00307911554039 26.557959606279752 23.18610790751066 72-73 23.246972987810572 26.971683675485842 26.523855044515955 23.25748829218763 74-75 23.224684647543928 27.021029388351046 26.525649073307033 23.228636890797993 76-77 23.243179049733893 27.135809194475176 26.410961608512157 23.21005014727877 78-79 23.21773854035176 27.126028549513826 26.37447816771592 23.281754742418496 80-81 23.19508794255144 27.167784380388355 26.36960513777874 23.26752253928146 82-83 23.196252128836495 27.286481228135745 26.323546951212844 23.19371969181492 84-85 23.166722965885217 27.265856353497497 26.30500034542431 23.262420335192974 86-87 23.168183122626505 27.355395660035658 26.261652408263625 23.21476880907421 88-89 23.17201373304241 27.448927946581094 26.211005332765453 23.168052987611045 90-91 23.168832972062276 27.463719500681762 26.126140654931458 23.241306872324508 92-93 23.134347478762887 27.5273463340585 26.123576152723043 23.21473003445557 94-95 23.148542103561887 27.616007091352856 26.065267427927214 23.170183377158043 96-97 23.108542159272304 27.643714400605667 26.02493143035459 23.22281200976743 98-99 23.08930006681139 27.740512584316612 25.99225893176666 23.177928417105335 100-101 23.101421286402342 27.859514476212766 25.6381370005979 23.400927236786988 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 83320.0 1 46687.5 2 7514.0 3 4281.5 4 3329.5 5 3011.0 6 3010.0 7 3094.0 8 3220.0 9 3382.5 10 3645.0 11 4133.0 12 5554.0 13 8638.0 14 12182.0 15 13818.5 16 34252.5 17 37876.0 18 20956.0 19 21312.0 20 23296.5 21 27391.5 22 33606.0 23 41904.5 24 52070.5 25 65246.5 26 81020.0 27 99695.5 28 120966.0 29 146576.5 30 180807.5 31 219238.5 32 261302.0 33 312346.0 34 361611.5 35 413048.0 36 475825.0 37 534834.5 38 579869.0 39 622248.5 40 670964.5 41 717325.5 42 764484.0 43 822064.5 44 876603.0 45 915243.5 46 936406.5 47 949226.5 48 962130.5 49 967621.5 50 963505.0 51 957340.0 52 946246.0 53 927652.0 54 885702.0 55 820154.0 56 747898.5 57 664415.0 58 584193.5 59 505506.0 60 421177.5 61 350338.5 62 291458.5 63 232723.0 64 182367.0 65 142000.5 66 105972.0 67 78027.5 68 59721.5 69 47087.0 70 38116.0 71 31083.5 72 25717.5 73 20097.0 74 14699.5 75 11504.0 76 8776.5 77 5872.0 78 3599.0 79 2120.0 80 1097.5 81 652.5 82 460.5 83 249.0 84 90.5 85 27.0 86 12.5 87 4.0 88 2.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09689244840745197 2 0.0036407249543765033 3 8.476658799479635E-6 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 6.357494099609727E-6 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.1191646998699087E-6 64-65 0.0016571867952982688 66-67 1.462223642910237E-4 68-69 8.540233740475732E-4 70-71 3.7721131657684375E-4 72-73 8.688575269466626E-5 74-75 2.754914109830881E-5 76-77 2.1191646998699087E-6 78-79 7.417076449544681E-4 80-81 0.002136118017468868 82-83 0.0013901720431146601 84-85 1.3562654079167416E-4 86-87 1.9072482298829177E-4 88-89 6.71775209858761E-4 90-91 8.095209153503051E-4 92-93 0.0032825861200984885 94-95 0.0016804976069968378 96-97 0.0020746622411726406 98-99 7.268734920553787E-4 100-101 5.509828219661762E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.3594202E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.335547808173665 #Duplication Level Percentage of deduplicated Percentage of total 1 74.94025867442029 39.969797492864444 2 13.435776869397076 14.332090391153635 3 4.8631598570326116 7.781378851606617 4 2.326614228854214 4.963649777369241 5 1.2536720304737567 3.343264225855161 6 0.7755317122312607 2.481804522867912 7 0.4984363742434197 1.8609063947454856 8 0.3464685231798645 1.478327078566959 9 0.23737912003306663 1.1394670864667238 >10 1.1677351673354506 11.020693881877701 >50 0.07763447163396692 2.921094916342691 >100 0.077008444830595 6.997873349591509 >500 2.1393450533908716E-4 0.06786185442400594 >1k 1.5798832732186636E-5 0.03959141213848916 >5k 3.949708179669685E-5 0.17249671111934892 >10k+ 5.529591451510071E-5 1.4297020530099565 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112585 0.4771723154697073 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95595 0.40516309896812785 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40992 0.1737375987541346 No Hit GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30858 0.13078636861717127 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28859 0.1223139481470914 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016571867952982686 0.0 0.0 0.0 0.0 2 0.0016826167716967075 4.238329399739817E-6 0.0 0.0 0.0 3 0.001695331759895927 4.238329399739817E-6 0.0 4.238329399739817E-6 0.0 4 0.0017038084186954067 4.238329399739817E-6 0.0 8.476658799479635E-6 4.238329399739817E-6 5 0.0017334767244935854 4.238329399739817E-6 0.0 8.476658799479635E-6 8.476658799479635E-6 6 0.0017461917126928047 4.238329399739817E-6 0.0 1.2714988199219452E-5 8.476658799479635E-6 7 0.0017928133360899429 4.238329399739817E-6 0.0 1.695331759895927E-5 1.2714988199219452E-5 8 0.001809766653688902 4.238329399739817E-6 0.0 2.1191646998699087E-5 2.1191646998699087E-5 9 0.0018563882770860401 8.476658799479635E-6 0.0 2.1191646998699087E-5 2.5429976398438904E-5 10-11 0.0019411548650808364 1.0595823499349543E-5 0.0 2.1191646998699087E-5 3.814496459765836E-5 12-13 0.0021128072057702988 1.483415289908936E-5 0.0 2.7549141098308815E-5 5.085995279687781E-5 14-15 0.0022759828876602823 1.695331759895927E-5 0.0 2.966830579817872E-5 5.721744689648754E-5 16-17 0.0024264435813510458 1.695331759895927E-5 0.0 2.966830579817872E-5 7.41707644954468E-5 18-19 0.002576904275041809 1.907248229882918E-5 0.0 2.966830579817872E-5 8.900491739453617E-5 20-21 0.0027527949451310115 2.1191646998699087E-5 0.0 3.602579989778845E-5 9.74815761940158E-5 22-23 0.0029308047799200838 2.1191646998699087E-5 0.0 4.026412929752827E-5 1.1231572909310516E-4 24-25 0.003149078744006684 2.3310811698568994E-5 0.0 4.6621623397137995E-5 1.1655405849284498E-4 26-27 0.0034012593432912034 2.5429976398438904E-5 0.0 5.085995279687781E-5 1.207923878925848E-4 28-29 0.003596222495679235 2.5429976398438904E-5 0.0 5.721744689648754E-5 1.229115525924547E-4 30-31 0.003812377295065966 2.966830579817872E-5 0.0 5.933661159635744E-5 1.2926904669206444E-4 32-33 0.004030651259152567 2.966830579817872E-5 0.0 6.357494099609726E-5 1.5257985839063344E-4 34-35 0.004486271669624597 3.178747049804863E-5 0.0 6.781327039583708E-5 1.6105651719011305E-4 36-37 0.005863728724540038 3.390663519791854E-5 0.0 6.781327039583708E-5 1.695331759895927E-4 38-39 0.006243059205816751 3.390663519791854E-5 0.0 6.781327039583708E-5 1.8436732888868208E-4 40-41 0.006624508851793335 3.390663519791854E-5 0.0 6.781327039583708E-5 1.9284398768816167E-4 42-43 0.007173372509059641 3.390663519791854E-5 0.0 6.993243509570699E-5 1.992014817877714E-4 44-45 0.007919318483413849 3.390663519791854E-5 0.0 7.20515997955769E-5 2.0343981118751123E-4 46-47 0.008703409422365716 3.390663519791854E-5 0.0 7.20515997955769E-5 2.161547993867307E-4 48-49 0.009684582678405482 3.390663519791854E-5 0.0 7.20515997955769E-5 2.3522728168555988E-4 50-51 0.010803501639936795 3.390663519791854E-5 0.0 7.20515997955769E-5 2.415847757851696E-4 52-53 0.01228691692984573 3.390663519791854E-5 0.0 7.628992919531672E-5 2.458231051849094E-4 54-55 0.014107279407033983 3.390663519791854E-5 0.0 8.264742329492644E-5 2.5429976398438903E-4 56-57 0.016137439189509356 3.390663519791854E-5 0.0 9.74815761940158E-5 2.691339168834784E-4 58-59 0.01825236555997952 3.390663519791854E-5 0.0 9.96007408938857E-5 2.8608723448243767E-4 60-61 0.020778409882224454 3.390663519791854E-5 0.0 1.0595823499349544E-4 2.903255638821775E-4 62-63 0.024003778555426458 3.814496459765836E-5 0.0 1.0807739969336535E-4 2.9668305798178724E-4 64-65 0.02786065830918969 3.814496459765836E-5 0.0 1.1019656439323526E-4 3.157555402806164E-4 66-67 0.03210746436772899 3.814496459765836E-5 0.0 1.1019656439323526E-4 3.1999386968035624E-4 68-69 0.03714683802401963 3.814496459765836E-5 0.0 1.1019656439323526E-4 3.369471872793155E-4 70-71 0.04819828193384121 3.814496459765836E-5 0.0 1.1443489379297507E-4 3.4966217547853496E-4 72-73 0.05990878606532232 3.814496459765836E-5 0.0 1.229115525924547E-4 3.6025799897788447E-4 74-75 0.0689109977103697 4.2383293997398174E-5 0.0 1.229115525924547E-4 3.793304812767137E-4 76-77 0.08043289618356239 4.2383293997398174E-5 0.0 1.3138821139193434E-4 3.9416463417580303E-4 78-79 0.0937560846516445 4.2383293997398174E-5 0.0 1.3562654079167416E-4 3.984029635755428E-4 80-81 0.10976213562976192 4.2383293997398174E-5 0.0 1.3562654079167416E-4 3.984029635755428E-4 82-83 0.12768179233186186 4.2383293997398174E-5 0.0 1.3562654079167416E-4 3.984029635755428E-4 84-85 0.14917012238854274 4.2383293997398174E-5 0.0 1.4198403489128387E-4 4.0476045767515254E-4 86-87 0.17394103856532211 4.4502458697268084E-5 0.0 1.4834152899089362E-4 4.153562811745021E-4 88-89 0.20337835541121502 4.6621623397137995E-5 0.0 1.4834152899089362E-4 4.195946105742419E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 13700 0.0 85.132385 1 TCGACGG 15435 0.0 75.64409 2 CGACGGC 18320 0.0 63.859 3 ATCTCGA 20690 0.0 58.4621 1 ACGGCGC 20795 0.0 56.418457 5 GACGGCG 20910 0.0 56.10817 4 CGGCGCG 22915 0.0 51.115944 6 TCTCGAG 25360 0.0 47.81909 2 CTCGAGG 31015 0.0 39.114006 3 TCGAGGG 31170 0.0 38.82807 4 TATCTCG 6430 0.0 38.118736 2 CGAGGGC 34050 0.0 35.711327 5 AGGGCGC 37340 0.0 32.463066 7 GGGCGCG 37355 0.0 32.38645 8 GAGGGCG 40310 0.0 30.14192 6 CGCGCCG 59740 0.0 28.00318 9 GGCGCGC 61675 0.0 27.170809 7 GCGCGCC 62405 0.0 26.868195 8 ATATCTC 11830 0.0 21.341629 1 CCATATT 34805 0.0 19.433523 18-19 >>END_MODULE