FastQCFastQC Report
Tue 31 May 2016
SRR1051280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23338766
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT22601789.684222379195198No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT1314650.5632902785005857No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT686160.2940001198006784No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351210.1504835345621958No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT256060.1097144553400981No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247150.10589677277710399No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA241240.10336450521848499No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236550.10135497309497854No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233580.10008241224064716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG2332950.094.0569461
TATCTCG2829000.092.485482
TCGACGG2394200.091.716192
ATATCTC2857750.091.671391
CGACGGC2416850.090.7819753
ACGGCGC2439050.089.901155
GACGGCG2440650.089.898664
ATCTCGA2927700.089.236153
CGGCGCG2461400.089.086766
TCTCGAG2928900.087.967114
CTCGAGG2987800.086.271125
TCGAGGG2993850.086.0444266
CGAGGGC3012700.085.480847
AGGGCGC3046550.084.621499
GAGGGCG3069350.084.079578
CTCGACG58800.060.5838785
TCTCGAC76550.046.846264
GGGCGCG3087350.041.87010610-11
TCCATAT2602650.041.6541918-19
CCATATT2624700.041.47797418-19
Produced by FastQC (version 0.11.5)