##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051280_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23338766 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.270167668676226 31.0 31.0 33.0 28.0 34.0 2 31.477440452507214 31.0 31.0 34.0 28.0 34.0 3 31.73967479685944 33.0 31.0 34.0 30.0 34.0 4 35.236241667618586 37.0 35.0 37.0 33.0 37.0 5 35.2640586481736 37.0 35.0 37.0 33.0 37.0 6 35.287679991307165 37.0 35.0 37.0 32.0 37.0 7 35.25119674279266 37.0 35.0 37.0 32.0 37.0 8 35.35391832627312 37.0 35.0 37.0 32.0 37.0 9 36.865339881294496 39.0 37.0 39.0 32.0 39.0 10-11 36.98969992672278 39.0 37.0 39.0 33.0 39.0 12-13 36.91591084978529 39.0 37.0 39.0 32.5 39.0 14-15 38.095637361461186 40.0 38.0 41.0 33.0 41.0 16-17 37.8434930107273 40.0 37.5 41.0 32.0 41.0 18-19 37.885469758769595 40.0 37.5 41.0 32.0 41.0 20-21 37.82335068615025 40.0 37.5 41.0 32.0 41.0 22-23 37.90586811659194 40.0 38.0 41.0 32.5 41.0 24-25 38.054407546654346 40.0 38.0 41.0 33.0 41.0 26-27 38.16024564023651 40.0 38.0 41.0 33.0 41.0 28-29 37.95362981059067 39.5 38.0 41.0 33.0 41.0 30-31 37.25053717493033 39.0 36.0 40.5 32.0 41.0 32-33 36.545351605136275 38.0 35.0 40.0 31.5 41.0 34-35 36.3153950812995 38.0 35.0 40.0 31.0 41.0 36-37 36.18673967166902 38.0 35.0 40.0 30.5 41.0 38-39 35.90437568550111 38.0 35.0 40.0 30.0 41.0 40-41 34.842727010502614 38.0 34.0 40.0 23.0 41.0 42-43 34.92561286230814 38.0 34.0 40.0 23.5 41.0 44-45 34.802969895666294 38.0 33.5 40.0 23.0 41.0 46-47 34.76512097940397 38.0 33.0 40.0 23.0 41.0 48-49 34.56614574223847 37.0 33.0 40.0 23.0 41.0 50-51 34.48921380419171 37.0 33.0 40.0 24.0 41.0 52-53 34.4798946525279 37.0 33.0 40.0 24.0 41.0 54-55 34.11310679407815 37.0 33.0 40.0 23.0 41.0 56-57 33.73818581496554 37.0 33.0 40.0 22.5 41.0 58-59 33.27893162817605 36.0 32.0 40.0 20.0 41.0 60-61 32.89833935521698 36.0 32.0 40.0 18.0 41.0 62-63 32.430888826770015 36.0 31.0 39.5 14.0 41.0 64-65 32.049971386662 35.0 31.0 39.0 9.0 41.0 66-67 31.547561083563714 35.0 31.0 39.0 7.0 41.0 68-69 30.96487768462137 35.0 30.0 38.5 3.5 40.0 70-71 30.466517252883037 35.0 30.0 37.5 2.0 40.0 72-73 29.94308533707395 35.0 29.0 37.0 2.0 39.0 74-75 29.47869750268716 35.0 29.0 36.5 2.0 39.0 76-77 28.928611242770934 34.0 28.5 36.0 2.0 39.0 78-79 28.565924586586966 34.0 28.0 35.5 2.0 37.5 80-81 28.1411070533892 34.0 27.0 35.0 2.0 37.0 82-83 27.725137331596706 34.0 26.0 35.0 2.0 36.5 84-85 27.36460158176315 34.0 26.0 35.0 2.0 36.0 86-87 27.030833185439196 34.0 25.0 35.0 2.0 36.0 88-89 26.6970768505927 34.0 25.0 35.0 2.0 35.5 90-91 26.37727596651854 34.0 24.0 35.0 2.0 35.0 92-93 26.096015616249804 34.0 23.5 35.0 2.0 35.0 94-95 25.772700086199933 33.5 20.5 35.0 2.0 35.0 96-97 25.38225549285682 33.0 18.5 35.0 2.0 35.0 98-99 24.95088223601882 33.0 4.5 35.0 2.0 35.0 100-101 23.75272880751279 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03833622850470775 1101 2 0.07114303974121583 1101 3 0.15522501739581784 1101 4 0.021233169884354197 1101 5 0.12827649325389956 1101 6 0.0848890774715727 1101 7 0.13922177493883225 1101 8 0.18614707825364007 1101 9 0.2405878042869105 1101 10-11 0.3648638431508928 1101 12-13 0.42600067054389257 1101 14-15 0.42196892113992135 1101 16-17 0.31091270245565994 1101 18-19 0.23499803685131582 1101 20-21 0.33201076512035144 1101 22-23 0.45108124852927034 1101 24-25 0.558117623058024 1101 26-27 0.4778209887541607 1101 28-29 0.5593823039761787 1101 30-31 0.6427006468631475 1101 32-33 0.5703135360655267 1101 34-35 0.5176162519550189 1101 36-37 0.5765450585661256 1101 38-39 0.5663759963511055 1101 40-41 0.5015568345799437 1101 42-43 0.43203723353497026 1101 44-45 0.37072913123223117 1101 46-47 0.2870736435589265 1101 48-49 0.27945194534639484 1101 50-51 0.1685476164050641 1101 52-53 0.026166907605464473 1101 54-55 -0.007427344223238208 1101 56-57 -0.1409819228296385 1101 58-59 -0.1842424609132962 1101 60-61 -0.16872281229016295 1101 62-63 -0.20166826988672426 1101 64-65 -0.26068510294465597 1101 66-67 -0.28490794373305306 1101 68-69 -0.2747249333017976 1101 70-71 -0.3382358456177137 1101 72-73 -0.35022325554237455 1101 74-75 -0.4127016105143113 1101 76-77 -0.39421547727034323 1101 78-79 -0.500237277405077 1101 80-81 -0.5511937308462294 1101 82-83 -0.6061720116190763 1101 84-85 -0.5955607398601579 1101 86-87 -0.5569400976790355 1101 88-89 -0.5337250757034866 1101 90-91 -0.5838366098905006 1101 92-93 -0.5549269318328172 1101 94-95 -0.584104721374775 1101 96-97 -0.6159153383816189 1101 98-99 -0.6434196428794259 1101 100-101 -0.4683778224541726 1102 1 -0.1271624874772357 1102 2 -0.023022864821001576 1102 3 0.08039580488908271 1102 4 -0.032712265954096154 1102 5 0.05183483285661339 1102 6 0.04496948316512572 1102 7 0.14013228568363445 1102 8 0.11945935362174964 1102 9 0.13777399093537923 1102 10-11 0.3038898678564337 1102 12-13 0.37417264589034716 1102 14-15 0.2982003415095278 1102 16-17 0.23541875365476983 1102 18-19 0.20274531644776062 1102 20-21 0.30323333980843614 1102 22-23 0.3766323372192346 1102 24-25 0.4590423175557987 1102 26-27 0.4314858863218163 1102 28-29 0.47858751658478127 1102 30-31 0.5112414861066767 1102 32-33 0.4516735149554876 1102 34-35 0.40848058109371266 1102 36-37 0.5134935945956869 1102 38-39 0.4399976071070242 1102 40-41 0.28145292599725735 1102 42-43 0.17447439349987093 1102 44-45 0.13409772654314622 1102 46-47 0.15458464855969112 1102 48-49 0.16544052154475253 1102 50-51 0.10074284051572135 1102 52-53 -0.03371165514543151 1102 54-55 -0.16461827890375957 1102 56-57 -0.21381325770085624 1102 58-59 -0.2756899521328222 1102 60-61 -0.23481943483552925 1102 62-63 -0.280655086009574 1102 64-65 -0.37809538860197023 1102 66-67 -0.4024008830690917 1102 68-69 -0.38607686597959656 1102 70-71 -0.5102539670517068 1102 72-73 -0.5112897277237991 1102 74-75 -0.5975294366817003 1102 76-77 -0.6699593962678563 1102 78-79 -0.695770300057454 1102 80-81 -0.6594535572146185 1102 82-83 -0.7199678080092298 1102 84-85 -0.725776745488492 1102 86-87 -0.800592884539622 1102 88-89 -0.7712772942214876 1102 90-91 -0.8223315000138172 1102 92-93 -0.8077707542607442 1102 94-95 -0.822878216736548 1102 96-97 -0.8766660007442688 1102 98-99 -0.8892594307105952 1102 100-101 -0.7505737699371657 1103 1 -0.07748556182579591 1103 2 5.248056455364747E-4 1103 3 0.08784920742135327 1103 4 0.01711330517175469 1103 5 0.05715544399942729 1103 6 0.0751886915775728 1103 7 0.1780119283731807 1103 8 0.1794593809385887 1103 9 0.22595384530760754 1103 10-11 0.30142467044881727 1103 12-13 0.37894754080256376 1103 14-15 0.2876428602422507 1103 16-17 0.27930003049203833 1103 18-19 0.2765854624002131 1103 20-21 0.32850097676629986 1103 22-23 0.3376001887530933 1103 24-25 0.4456800436405075 1103 26-27 0.4108968816860781 1103 28-29 0.4832538861842721 1103 30-31 0.5015112074404229 1103 32-33 0.4249039401349677 1103 34-35 0.3693084949761243 1103 36-37 0.42945070804262286 1103 38-39 0.29973637457455027 1103 40-41 0.17475035908756809 1103 42-43 0.06703169381277974 1103 44-45 0.03645763428082205 1103 46-47 0.040103560585912135 1103 48-49 -0.03302333535032176 1103 50-51 -0.04991568250191847 1103 52-53 -0.15015513034675365 1103 54-55 -0.23984276696251783 1103 56-57 -0.3480399915251695 1103 58-59 -0.3776877032494923 1103 60-61 -0.380997393792299 1103 62-63 -0.4188319061369583 1103 64-65 -0.5308508482052261 1103 66-67 -0.5589724935506055 1103 68-69 -0.6040197831352927 1103 70-71 -0.7183632444564445 1103 72-73 -0.7311887507407846 1103 74-75 -0.7748429487177084 1103 76-77 -0.6826342127634639 1103 78-79 -0.8201512812681742 1103 80-81 -0.9041252179912433 1103 82-83 -0.9637524294318744 1103 84-85 -0.9562960219846879 1103 86-87 -0.9820575367455184 1103 88-89 -0.9479848178972929 1103 90-91 -1.0268642529816034 1103 92-93 -1.005561334492942 1103 94-95 -1.0830844093349015 1103 96-97 -1.0919930645169522 1103 98-99 -1.1564059857590685 1103 100-101 -1.0088122496253504 1104 1 -0.20694054809230167 1104 2 -0.14133447235026608 1104 3 0.0052094478844502135 1104 4 -0.1625344206735022 1104 5 -0.07823868612977236 1104 6 -0.02385866305243667 1104 7 0.10006671712753956 1104 8 0.1065026891942793 1104 9 0.05038372008525016 1104 10-11 0.12352977029913603 1104 12-13 0.19866659718712043 1104 14-15 0.08793897098219361 1104 16-17 0.10638536771840279 1104 18-19 0.10455591988011292 1104 20-21 0.167003697654728 1104 22-23 0.2149019264342087 1104 24-25 0.2345766721392053 1104 26-27 0.2324165100805331 1104 28-29 0.1853659788806965 1104 30-31 0.25139711134065124 1104 32-33 0.1694897687448389 1104 34-35 -0.016915066214707508 1104 36-37 0.13939187434331757 1104 38-39 0.10268819508944205 1104 40-41 0.03733869841543935 1104 42-43 -0.09948550058134487 1104 44-45 -0.097751865828414 1104 46-47 -0.16463924010487574 1104 48-49 -0.17845149206495847 1104 50-51 -0.2174794187356497 1104 52-53 -0.395245630570507 1104 54-55 -0.3715310539326069 1104 56-57 -0.44244309385027236 1104 58-59 -0.370127549666897 1104 60-61 -0.4064235000030081 1104 62-63 -0.4888388844364755 1104 64-65 -0.5309697328395551 1104 66-67 -0.5836984841238717 1104 68-69 -0.5844632882206113 1104 70-71 -0.7893071399012577 1104 72-73 -0.7970959161664766 1104 74-75 -0.8170029299079964 1104 76-77 -0.6820970776429327 1104 78-79 -0.7356746122988831 1104 80-81 -0.7781231302407363 1104 82-83 -0.9356083105038806 1104 84-85 -1.0085905117146226 1104 86-87 -0.9897858747497672 1104 88-89 -0.9046690079845021 1104 90-91 -0.9832775338088666 1104 92-93 -1.033666724029203 1104 94-95 -1.0376610180020904 1104 96-97 -1.098617275609186 1104 98-99 -1.2011525753268408 1104 100-101 -1.0469780285400354 1105 1 0.17014736600931712 1105 2 0.024375926819875104 1105 3 0.14031444390806058 1105 4 -0.0758108123965826 1105 5 0.18684165959143684 1105 6 0.12892310783708183 1105 7 0.12721573225191207 1105 8 0.07904247297506117 1105 9 0.09434032115434832 1105 10-11 0.06818910288327373 1105 12-13 0.13789998681605908 1105 14-15 0.051308730347471965 1105 16-17 0.14502277144342202 1105 18-19 0.1336932892403837 1105 20-21 0.0780161494841991 1105 22-23 0.062386538721639795 1105 24-25 0.18897987085198054 1105 26-27 0.09722206352062557 1105 28-29 0.13092302422845847 1105 30-31 0.14096346845166607 1105 32-33 0.11102527487656744 1105 34-35 0.007847446905508093 1105 36-37 -0.011650971811064892 1105 38-39 0.053112691791596944 1105 40-41 0.10362305249788051 1105 42-43 -0.08752184796406226 1105 44-45 -0.14121320417993388 1105 46-47 -0.19618694633383882 1105 48-49 -0.21102217051750216 1105 50-51 -0.12616527433371516 1105 52-53 -0.25605547046887267 1105 54-55 -0.38828891247784725 1105 56-57 -0.5384394929903138 1105 58-59 -0.5567415442020831 1105 60-61 -0.5799040922988468 1105 62-63 -0.5902801865730396 1105 64-65 -0.6681523327805365 1105 66-67 -0.7196282504043197 1105 68-69 -0.7634388072824976 1105 70-71 -0.8091880135654641 1105 72-73 -0.8779653322391852 1105 74-75 -0.9774799002956058 1105 76-77 -0.8810084352766197 1105 78-79 -0.8471738733135581 1105 80-81 -0.9025260390348393 1105 82-83 -1.0034615228596167 1105 84-85 -1.0068704580552073 1105 86-87 -1.0896074341150417 1105 88-89 -1.0485368970408757 1105 90-91 -1.073661419595858 1105 92-93 -1.0980681587453205 1105 94-95 -1.1642059865578425 1105 96-97 -1.2691401254761523 1105 98-99 -1.3355178617510397 1105 100-101 -1.2777971192334832 1106 1 -0.04036966931438002 1106 2 -0.14416033605337475 1106 3 -0.004418658467312042 1106 4 -0.031920347505597135 1106 5 -0.04096827386141655 1106 6 -0.027505310207992295 1106 7 0.007517011926353234 1106 8 -0.08969097857227837 1106 9 -0.2553491093157305 1106 10-11 -0.23360821740589444 1106 12-13 -0.12603162143057745 1106 14-15 -0.21325230667925155 1106 16-17 -0.08871285664125139 1106 18-19 -0.06829385377727704 1106 20-21 -0.19525688161352406 1106 22-23 -0.19052262793572083 1106 24-25 -0.09327926632919059 1106 26-27 -0.1399548194429414 1106 28-29 -0.060550009301209684 1106 30-31 -0.017384411790828835 1106 32-33 -0.08153068163652222 1106 34-35 -0.14576927244775106 1106 36-37 -0.22577150746906227 1106 38-39 -0.157659194456933 1106 40-41 -0.1735130687604567 1106 42-43 -0.2824156875466528 1106 44-45 -0.3005622979417666 1106 46-47 -0.38557745365751117 1106 48-49 -0.3953133783161391 1106 50-51 -0.46780642675760475 1106 52-53 -0.5027713442271349 1106 54-55 -0.6051519697374488 1106 56-57 -0.6353987816147892 1106 58-59 -0.8607762148169797 1106 60-61 -0.7954712140972049 1106 62-63 -0.7970094155085192 1106 64-65 -0.8770882133459637 1106 66-67 -0.8894621057678904 1106 68-69 -1.0383132557731294 1106 70-71 -1.0866831284398408 1106 72-73 -1.0597562181887668 1106 74-75 -1.1662783971482291 1106 76-77 -1.2043127179624555 1106 78-79 -1.2469078917871883 1106 80-81 -1.2232268149466634 1106 82-83 -1.4023005513223694 1106 84-85 -1.3622428676275113 1106 86-87 -1.424501853552048 1106 88-89 -1.4651630106038347 1106 90-91 -1.5077990073694494 1106 92-93 -1.5235440819084047 1106 94-95 -1.5927953667636956 1106 96-97 -1.7567736348141771 1106 98-99 -1.7875937591274358 1106 100-101 -1.7009623503945654 1107 1 -0.01796476718685014 1107 2 -0.2310484862358102 1107 3 -0.1854777531555385 1107 4 -0.2103500485000822 1107 5 -0.09546829788875755 1107 6 -0.10397462190653073 1107 7 -0.10614895462021678 1107 8 -0.18873329794103455 1107 9 -0.2469153312164849 1107 10-11 -0.2501603264389587 1107 12-13 -0.19959313025762526 1107 14-15 -0.28896557200391726 1107 16-17 -0.22734038330916917 1107 18-19 -0.21741492999462508 1107 20-21 -0.36710555656039645 1107 22-23 -0.3980752199435642 1107 24-25 -0.27952331446944356 1107 26-27 -0.33209189363928004 1107 28-29 -0.26615963470020176 1107 30-31 -0.2608668635927387 1107 32-33 -0.36291425898845375 1107 34-35 -0.4497239994783655 1107 36-37 -0.346658460658972 1107 38-39 -0.3739912276727324 1107 40-41 -0.3522221941360755 1107 42-43 -0.48895688295048956 1107 44-45 -0.445717682790864 1107 46-47 -0.4289931959653899 1107 48-49 -0.47256231940315274 1107 50-51 -0.5244486143633154 1107 52-53 -0.5048611429752938 1107 54-55 -0.6930384237874136 1107 56-57 -0.752031663813618 1107 58-59 -0.8293065525228087 1107 60-61 -0.9577102713866026 1107 62-63 -0.9801982991609606 1107 64-65 -1.3200460600706876 1107 66-67 -1.14944824074297 1107 68-69 -1.1055385389942813 1107 70-71 -1.1414694167693362 1107 72-73 -1.2127144642252503 1107 74-75 -1.2047252429708095 1107 76-77 -1.2590837015599696 1107 78-79 -1.3179711184613758 1107 80-81 -1.3572458792590254 1107 82-83 -1.5335952496176084 1107 84-85 -1.477797279704017 1107 86-87 -1.5207553090287966 1107 88-89 -1.5746375956737815 1107 90-91 -1.6113096042314652 1107 92-93 -1.6168037298985638 1107 94-95 -1.801721658787315 1107 96-97 -1.8587622381080884 1107 98-99 -1.9173109256905292 1107 100-101 -1.841438546190055 1108 1 -0.42181264106940475 1108 2 -0.31362342097011364 1108 3 -0.2426473473970141 1108 4 -0.14446039327622628 1108 5 -0.14864913314779216 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-2.6420966493371516 2308 76-77 -3.111240837975 2308 78-79 -2.817559556717356 2308 80-81 -3.075617509894073 2308 82-83 -3.1458964106555136 2308 84-85 -3.4533233012539704 2308 86-87 -3.7680558634247525 2308 88-89 -3.9960832337223593 2308 90-91 -4.36328576187416 2308 92-93 -4.063450435383565 2308 94-95 -4.4176326150371 2308 96-97 -4.760364271223718 2308 98-99 -4.72799877209647 2308 100-101 -4.479721669340279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 518.0 11 4821.0 12 15913.0 13 32630.0 14 51878.0 15 74607.0 16 102893.0 17 155548.0 18 264975.0 19 518704.0 20 557766.0 21 497785.0 22 461288.0 23 439577.0 24 446654.0 25 472678.0 26 516142.0 27 549602.0 28 587171.0 29 629924.0 30 648087.0 31 698646.0 32 802701.0 33 969665.0 34 1264904.0 35 1885874.0 36 2943834.0 37 4232500.0 38 3162240.0 39 349178.0 40 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.278773007964517 35.65490994682409 19.550836578077867 17.515480467133525 2 19.067032078731156 23.04246077106219 42.16744364290725 15.7230635072994 3 18.819662530572526 33.24026643053879 20.446385211626016 27.493685827262677 4 29.121201180902194 20.779534787743277 32.147822211337136 17.95144182001739 5 19.15877643231009 31.125887289842147 19.92429676873233 29.791039509115436 6 19.257227192612806 20.781939404248682 31.02502210378313 28.935811299355386 7 29.14430865796418 20.877916167461468 19.9355698583207 30.04220531625365 8 40.48816891175823 20.776132722698364 19.98139061850999 18.754307747033412 9 18.81857849725217 32.143001905070726 20.277751617202043 28.76066798047506 10-11 35.44069767870332 20.598976826795383 20.215876023608104 23.74444947089319 12-13 29.48480866554813 20.581940793270732 20.40038663569445 29.53286390548669 14-15 29.249067838462413 20.592560463565214 20.52473554085936 29.63363615711302 16-17 29.303118625656516 25.537029132498425 20.485641903110366 24.674210338734696 18-19 24.241492641105904 20.69587658130154 25.707932703379978 29.354698074212582 20-21 23.941893500281893 31.281726720255904 21.177972734291092 23.59840704517111 22-23 17.971526429460752 25.28618908129076 32.278109733822255 24.464174755426228 24-25 17.978469812842718 20.57504454177226 37.267328101237226 24.17915754414779 26-27 17.97316104887465 20.544997966044992 43.30188879737686 18.179952187703496 28-29 17.951336190488085 20.33237727545421 43.54624725662434 18.170039277433368 30-31 17.99650587603521 20.292662232555315 43.5276760900638 18.183155801345677 32-33 18.011796001040384 20.30164451568865 43.495535310660024 18.191024172610945 34-35 18.0084045627046 20.348608384351373 43.5053148150358 18.137672237908227 36-37 18.045738870618845 20.342224535319616 43.38346752699836 18.228569067063184 38-39 18.33832853922395 20.499249080208415 42.526965178154015 18.635457202413622 40-41 19.686941444504196 21.700123035524122 38.47260359506935 20.140331924902334 42-43 19.844546289411717 22.123512271963396 38.431965039090095 19.599976399534793 44-45 20.062702896006375 22.91285303532865 37.502250874013974 19.522193194651 46-47 19.743996053857373 23.635674404498644 37.23471750261824 19.385612039025744 48-49 19.71998047625955 23.95355915461767 36.7478112756221 19.578649093500683 50-51 19.799557782581807 24.191029800829916 36.337166529855544 19.672245886732732 52-53 19.89522752316515 24.197347599214908 36.07458309262943 19.83284178499051 54-55 20.245375943082493 24.072359631461122 35.63011212378516 20.052152301671224 56-57 20.554945803241967 23.9669936363025 35.37638528159535 20.10167527886019 58-59 20.70856196628091 24.081100578044193 35.01667898258806 20.193658473086835 60-61 20.824692887518143 24.19563916739877 34.529407766035376 20.45026017904771 62-63 20.989207455563943 24.439673256921207 33.85339220206915 20.717727085445702 64-65 21.17388297221884 24.6504849485187 33.268946181644736 20.906685897617724 66-67 21.4255231137735 24.804868983858462 32.650587944634815 21.11901995773322 68-69 21.520250102737233 24.983769113244357 32.21349659481856 21.282484189199852 70-71 21.4931120156994 25.176135652106467 31.885816623868397 21.444935708325733 72-73 21.622759866229334 25.30057051775075 31.53000949077595 21.546660125243964 74-75 21.829419825555217 25.379955522188798 31.199807832134546 21.590816820121443 76-77 21.92198662669756 25.49902216118981 30.836461070634623 21.742530141478007 78-79 22.090041941722262 25.42408309165549 30.5478704022443 21.938004564377948 80-81 22.1795333962732 25.521253903408052 30.259406497295206 22.03980620302354 82-83 22.323071378521686 25.622763030122574 29.97288438261185 22.081281208743892 84-85 22.392418191820042 25.64892381931822 29.799396406534544 22.159261582327197 86-87 22.557529407447163 25.81583078401556 29.452013135328897 22.17462667320838 88-89 22.634307363816994 25.896415938735174 29.198265411568414 22.271011285879418 90-91 22.82651757095412 25.881480597242078 28.840662976550924 22.451338855252878 92-93 22.839063442442757 25.945059131390764 28.69903741948056 22.516840006685914 94-95 22.927306536637797 26.08416314740918 28.47227222579201 22.51625809016102 96-97 23.008466622250953 26.052294854011514 28.30228968017644 22.636948843561093 98-99 23.145647487247007 26.11064181318153 28.13633782081179 22.607372878759673 100-101 23.59989559804536 25.853661974827286 27.762502563613005 22.783939863514348 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 17217.0 1 9948.0 2 2176.0 3 1429.0 4 1112.0 5 1029.0 6 1068.5 7 1133.5 8 1207.5 9 1254.5 10 1329.5 11 1479.5 12 2014.0 13 3589.0 14 5638.0 15 10226.0 16 214865.5 17 266913.5 18 112435.5 19 105225.0 20 103270.5 21 105778.0 22 112534.0 23 121900.0 24 133640.0 25 148389.5 26 166278.0 27 188109.0 28 213532.0 29 244572.5 30 284693.5 31 331414.5 32 382799.5 33 439138.5 34 488045.5 35 527623.0 36 571353.5 37 615347.0 38 647289.5 39 672212.0 40 700252.0 41 725875.5 42 753036.0 43 793720.0 44 835606.5 45 861033.5 46 862068.0 47 852728.5 48 845819.0 49 834911.5 50 818240.0 51 800241.0 52 780201.0 53 754833.0 54 713477.5 55 658450.5 56 598865.0 57 529880.0 58 461087.5 59 393807.5 60 324926.0 61 266090.5 62 217032.5 63 169515.5 64 130570.0 65 100342.0 66 74133.5 67 53969.0 68 40251.0 69 31105.5 70 24879.0 71 20065.5 72 16420.0 73 12701.5 74 9297.5 75 7341.0 76 5623.0 77 3796.5 78 2362.0 79 1484.5 80 900.5 81 536.0 82 307.0 83 173.5 84 91.5 85 39.0 86 20.0 87 7.5 88 4.0 89 3.5 90 3.5 91 2.5 92 2.5 93 3.5 94 4.5 95 3.5 96 1.5 97 2.0 98 2.5 99 2.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 4.284716681250414E-6 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.7705506795003644E-4 18-19 4.284716681250414E-6 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.9923932567814424E-4 30-31 6.641310855938141E-4 32-33 0.005124521150775495 34-35 0.0015917722470845288 36-37 7.305441941531957E-4 38-39 2.3565941746877275E-4 40-41 0.001647473563940784 42-43 0.0010411861535438505 44-45 0.0026115348172221276 46-47 0.00227946927442522 48-49 2.142358340625207E-6 50-51 8.569433362500829E-5 52-53 4.220445931031658E-4 54-55 2.99930167687529E-5 56-57 2.3351705912814756E-4 58-59 0.0017653032726751706 60-61 5.420166601781774E-4 62-63 2.6565243423752567E-4 64-65 0.0 66-67 0.0014739425383501425 68-69 0.0056408295108661705 70-71 0.0067205781145412745 72-73 0.0013539704712751308 74-75 0.0035863078622065964 76-77 0.0046060704323441955 78-79 0.007461834100397596 80-81 0.004914570033394225 82-83 0.0023780177580939796 84-85 0.0016089111138095304 86-87 0.0014246682965157627 88-89 3.7919742629066163E-4 90-91 9.426376698750911E-5 92-93 6.29853352143811E-4 94-95 6.61988727253189E-4 96-97 7.669642859438241E-4 98-99 5.184507184313001E-4 100-101 5.248777934531758E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.3338766E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.51582323848484 #Duplication Level Percentage of deduplicated Percentage of total 1 75.78048634319116 36.76552680352677 2 12.863349813186161 12.481520115826736 3 4.750526334821061 6.914270878498375 4 2.2747337163100934 4.414423155804887 5 1.214033077652596 2.9449907100533546 6 0.7808192901166802 2.272925439630004 7 0.5114895199179951 1.7370734595675243 8 0.3412821298649837 1.3246066789586541 9 0.23537738068966702 1.0277574656289696 >10 1.0961455622936414 9.212224798828492 >50 0.08150046530398311 2.8271133023773176 >100 0.0683010841396647 5.701623526185565 >500 9.546345839663737E-4 0.32024993684905595 >1k 7.712331966915644E-4 0.8529356200684927 >5k 9.832059924188515E-5 0.34910409586465735 >10k+ 1.310941323221874E-4 10.85365401233113 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 2260178 9.684222379195198 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 131465 0.5632902785005857 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68616 0.2940001198006784 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35121 0.1504835345621958 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 25606 0.1097144553400981 No Hit GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24715 0.10589677277710399 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 24124 0.10336450521848499 No Hit GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23655 0.10135497309497854 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23358 0.10008241224064716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.005253062651213007 0.0 0.0 0.0 4.284716681250414E-6 2 0.005265916801256759 0.0 0.0 8.569433362500828E-6 8.569433362500828E-6 3 0.005300194534706762 0.0 0.0 8.569433362500828E-6 8.569433362500828E-6 4 0.005308763968069263 0.0 0.0 8.569433362500828E-6 1.2854150043751242E-5 5 0.005343041701519266 0.0 0.0 1.2854150043751242E-5 1.7138866725001656E-5 6 0.0053901735850130205 0.0 0.0 1.2854150043751242E-5 1.7138866725001656E-5 7 0.005437305468506775 0.0 0.0 1.2854150043751242E-5 2.142358340625207E-5 8 0.0054887220686817804 0.0 0.0 2.142358340625207E-5 2.142358340625207E-5 9 0.005831499403181814 0.0 0.0 2.142358340625207E-5 2.5708300087502484E-5 10-11 0.005970752695322452 0.0 0.0 2.5708300087502484E-5 3.642009179062852E-5 12-13 0.006202127396109975 0.0 0.0 2.5708300087502484E-5 4.284716681250414E-5 14-15 0.006433502096897496 0.0 0.0 2.5708300087502484E-5 4.284716681250414E-5 16-17 0.006697012172794397 4.284716681250414E-6 0.0 2.99930167687529E-5 5.3558958515630173E-5 18-19 0.007024792998910054 4.284716681250414E-6 0.0 3.8562450131253724E-5 5.99860335375058E-5 20-21 0.007337577316641334 4.284716681250414E-6 0.0 5.141660017500497E-5 6.427075021875621E-5 22-23 0.007751052476381999 4.284716681250414E-6 0.0 5.141660017500497E-5 7.498254192188224E-5 24-25 0.008162385277782038 4.284716681250414E-6 0.0 5.141660017500497E-5 8.140961694375786E-5 26-27 0.008610138170972708 6.427075021875621E-6 0.0 5.141660017500497E-5 8.99790503062587E-5 28-29 0.009079314647569628 8.569433362500828E-6 0.0 5.570131685625538E-5 9.854848366875952E-5 30-31 0.009582768857616551 8.569433362500828E-6 0.0 5.99860335375058E-5 1.0926027537188555E-4 32-33 0.010019809959104092 8.569433362500828E-6 0.0 6.2128391878131E-5 1.199720670750116E-4 34-35 0.010653948027929155 8.569433362500828E-6 0.0 6.641310855938142E-5 1.3068385877813761E-4 36-37 0.011870807565404273 8.569433362500828E-6 0.0 7.069782524063183E-5 1.4139565048126366E-4 38-39 0.01245781375073558 1.0711791703126034E-5 0.0 7.498254192188224E-5 1.542498005250149E-4 40-41 0.013029823427682509 1.2854150043751242E-5 0.0 7.712490026250745E-5 1.563921588656401E-4 42-43 0.013777506488560708 1.2854150043751242E-5 0.0 7.712490026250745E-5 1.6067687554689053E-4 44-45 0.014681581708304544 1.2854150043751242E-5 0.0 8.569433362500829E-5 1.6710395056876616E-4 46-47 0.015656354753289012 1.2854150043751242E-5 0.0 8.99790503062587E-5 1.7781574227189218E-4 48-49 0.01670825269853599 1.2854150043751242E-5 0.0 8.99790503062587E-5 1.8210045895314262E-4 50-51 0.017788001302211094 1.7138866725001656E-5 0.0 8.99790503062587E-5 1.9066989231564344E-4 52-53 0.019289794498989363 1.7138866725001656E-5 0.0 9.426376698750911E-5 1.9281225065626863E-4 54-55 0.02118578163044267 1.7138866725001656E-5 0.0 1.0497555869063514E-4 1.9281225065626863E-4 56-57 0.02315460894547724 1.7138866725001656E-5 0.0 1.1140263371251076E-4 1.9495460899689383E-4 58-59 0.02523698125256494 1.7138866725001656E-5 0.0 1.1140263371251076E-4 1.9923932567814426E-4 60-61 0.027951349270137076 1.7138866725001656E-5 0.0 1.1140263371251076E-4 2.0138168401876946E-4 62-63 0.031182025647799885 1.7138866725001656E-5 0.0 1.1140263371251076E-4 2.0566640070001987E-4 64-65 0.03526536064503153 1.7138866725001656E-5 0.0 1.1354499205313597E-4 2.163781924031459E-4 66-67 0.040038535027944494 1.7138866725001656E-5 0.0 1.1568735039376118E-4 2.2494762576564674E-4 68-69 0.04564508680536066 1.7138866725001656E-5 0.0 1.1568735039376118E-4 2.2708998410627193E-4 70-71 0.05651112830901171 1.7138866725001656E-5 0.0 1.1568735039376118E-4 2.3351705912814756E-4 72-73 0.06815056117362846 1.7138866725001656E-5 0.0 1.1568735039376118E-4 2.3565941746877278E-4 74-75 0.07835032923334506 1.7138866725001656E-5 0.0 1.1568735039376118E-4 2.3565941746877278E-4 76-77 0.0909902434430338 1.7138866725001656E-5 0.0 1.1568735039376118E-4 2.37801775809398E-4 78-79 0.10600603305247586 1.9281225065626862E-5 0.0 1.221144254156368E-4 2.399441341500232E-4 80-81 0.12400827018874949 2.142358340625207E-5 0.0 1.24256783756262E-4 2.442288508312736E-4 82-83 0.14503337494364527 2.142358340625207E-5 0.0 1.24256783756262E-4 2.442288508312736E-4 84-85 0.17042032128005397 2.142358340625207E-5 0.0 1.2854150043751242E-4 2.4637120917189877E-4 86-87 0.20066613633300065 2.142358340625207E-5 0.0 1.2854150043751242E-4 2.5279828419377446E-4 88-89 0.23655278089681347 2.142358340625207E-5 0.0 1.3496857545938805E-4 2.6136771755627523E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 233295 0.0 94.056946 1 TATCTCG 282900 0.0 92.48548 2 TCGACGG 239420 0.0 91.71619 2 ATATCTC 285775 0.0 91.67139 1 CGACGGC 241685 0.0 90.781975 3 ACGGCGC 243905 0.0 89.90115 5 GACGGCG 244065 0.0 89.89866 4 ATCTCGA 292770 0.0 89.23615 3 CGGCGCG 246140 0.0 89.08676 6 TCTCGAG 292890 0.0 87.96711 4 CTCGAGG 298780 0.0 86.27112 5 TCGAGGG 299385 0.0 86.044426 6 CGAGGGC 301270 0.0 85.48084 7 AGGGCGC 304655 0.0 84.62149 9 GAGGGCG 306935 0.0 84.07957 8 CTCGACG 5880 0.0 60.583878 5 TCTCGAC 7655 0.0 46.84626 4 GGGCGCG 308735 0.0 41.870106 10-11 TCCATAT 260265 0.0 41.65419 18-19 CCATATT 262470 0.0 41.477974 18-19 >>END_MODULE