FastQCFastQC Report
Tue 31 May 2016
SRR1051280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23338766
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680220.719926666217057No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1034640.4433139267088928No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452380.19383201322640622No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342750.14685866424985794No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336030.14397933464005766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG151650.085.2525251
TCGACGG167400.077.409392
CGACGGC197900.065.619983
ATCTCGA218450.058.943661
ACGGCGC226650.057.4429475
GACGGCG229250.056.8121874
CGGCGCG257000.050.733246
TCTCGAG269500.047.836112
CTCGAGG340700.037.907093
TCGAGGG342200.037.6715244
TATCTCG66950.035.9717982
CGAGGGC378450.034.2263035
AGGGCGC417550.030.8506687
GGGCGCG417450.030.8239238
GAGGGCG442050.029.2804996
CGCGCCG672150.027.6944319
GGCGCGC685500.027.18287
GCGCGCC695800.026.807728
ATATCTC122350.020.2087671
CCATATT365350.020.19043718-19
Produced by FastQC (version 0.11.5)