##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051280_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23338766 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19329029649639 33.0 31.0 34.0 31.0 34.0 2 32.47649117352648 34.0 31.0 34.0 31.0 34.0 3 32.49479424062095 34.0 31.0 34.0 31.0 34.0 4 36.15452672176412 37.0 35.0 37.0 35.0 37.0 5 36.12065907854768 37.0 35.0 37.0 35.0 37.0 6 36.10684463780133 37.0 35.0 37.0 35.0 37.0 7 36.1037302486344 37.0 35.0 37.0 35.0 37.0 8 36.099231938826584 37.0 35.0 37.0 35.0 37.0 9 37.81885983174946 39.0 38.0 39.0 35.0 39.0 10-11 37.810847754332855 39.0 38.0 39.0 35.0 39.0 12-13 37.789031990808766 39.0 38.0 39.0 35.0 39.0 14-15 39.20139453816881 40.0 39.0 41.0 36.0 41.0 16-17 39.17234160966351 40.0 39.0 41.0 36.0 41.0 18-19 39.14810241038451 40.0 38.5 41.0 36.0 41.0 20-21 39.1432766839515 40.0 39.0 41.0 36.0 41.0 22-23 39.120792440354386 40.0 39.0 41.0 36.0 41.0 24-25 39.06797546622645 40.0 38.5 41.0 35.5 41.0 26-27 38.924999526538805 40.0 38.0 41.0 35.0 41.0 28-29 38.750522135574776 40.0 38.0 41.0 35.0 41.0 30-31 38.54900385478821 40.0 38.0 41.0 35.0 41.0 32-33 38.59955414952101 40.0 38.0 41.0 35.0 41.0 34-35 38.594425814972396 40.0 38.0 41.0 35.0 41.0 36-37 38.57387061509593 40.0 38.0 41.0 35.0 41.0 38-39 38.50614653319717 40.0 38.0 41.0 35.0 41.0 40-41 38.36258819339463 40.0 38.0 41.0 34.0 41.0 42-43 38.269776624008315 40.0 38.0 41.0 34.0 41.0 44-45 38.16549274713154 40.0 38.0 41.0 33.5 41.0 46-47 38.02928554577393 40.0 37.0 41.0 33.0 41.0 48-49 37.949529700927634 40.0 37.0 41.0 33.0 41.0 50-51 37.951988292782914 40.0 37.0 41.0 33.0 41.0 52-53 37.823923145722446 40.0 37.0 41.0 33.0 41.0 54-55 37.71627077455595 40.0 36.5 41.0 33.0 41.0 56-57 37.53047793957916 39.0 36.0 41.0 33.0 41.0 58-59 37.28142132707445 39.0 35.5 41.0 33.0 41.0 60-61 37.05190334398999 39.0 35.0 41.0 32.0 41.0 62-63 36.689407829017185 38.5 35.0 40.0 32.0 41.0 64-65 36.32952695528118 38.0 35.0 40.0 31.0 41.0 66-67 35.93815913403476 37.0 35.0 40.0 31.0 41.0 68-69 35.54987493768951 36.5 35.0 39.0 31.0 41.0 70-71 35.14494633092426 36.0 34.0 39.0 30.0 40.5 72-73 34.77644452153126 35.5 34.0 38.5 30.0 40.0 74-75 34.40530165562309 35.0 34.0 37.0 30.0 39.0 76-77 33.832908689345444 35.0 33.5 37.0 29.0 39.0 78-79 33.61574245185028 35.0 34.0 36.0 29.0 39.0 80-81 33.27345106849265 35.0 33.0 36.0 29.0 37.0 82-83 32.92700781609447 35.0 33.0 35.5 29.0 37.0 84-85 32.68645844428964 35.0 33.0 35.0 28.5 36.5 86-87 32.421832992369865 35.0 33.0 35.0 27.5 36.0 88-89 32.18357003108048 35.0 33.0 35.0 27.0 36.0 90-91 31.970928390129963 35.0 33.0 35.0 27.0 36.0 92-93 31.73719321749916 35.0 32.5 35.0 26.0 35.0 94-95 31.539147828124246 34.0 32.0 35.0 25.0 35.0 96-97 31.347244772924157 34.0 32.0 35.0 25.0 35.0 98-99 31.03064242985255 34.0 32.0 35.0 24.5 35.0 100-101 29.54075318292321 33.5 29.5 34.5 12.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2195402551283152 1101 2 0.17217827246435746 1101 3 0.06391336141252424 1101 4 0.10551465165348617 1101 5 0.2038254351164639 1101 6 0.24428373299986106 1101 7 0.2718400509180441 1101 8 0.2769244189648177 1101 9 0.3754704922549976 1101 10-11 0.36397204873281197 1101 12-13 0.36889456182745306 1101 14-15 0.46401653790845643 1101 16-17 0.41757870853179924 1101 18-19 0.40663940059561554 1101 20-21 0.40222033311229666 1101 22-23 0.4092380361597847 1101 24-25 0.4344717592796883 1101 26-27 0.46836900010335825 1101 28-29 0.48441190090776587 1101 30-31 0.4724343267811051 1101 32-33 0.45647255458831637 1101 34-35 0.4461590095279604 1101 36-37 0.38314136035102564 1101 38-39 0.343902728875328 1101 40-41 0.3204742126301241 1101 42-43 0.2520385627366224 1101 44-45 0.25390689523445786 1101 46-47 0.2263344556734026 1101 48-49 0.25699412166856916 1101 50-51 0.2887050291322879 1101 52-53 0.3236155837328525 1101 54-55 0.4073002236173835 1101 56-57 0.3678414239894181 1101 58-59 0.347102561300062 1101 60-61 0.32316162167391127 1101 62-63 0.32390762228678227 1101 64-65 0.3562284990880755 1101 66-67 0.32855830130282015 1101 68-69 0.2759362164997299 1101 70-71 0.24483024134620024 1101 72-73 0.20348999001101475 1101 74-75 0.1058551549257345 1101 76-77 0.07437367048439825 1101 78-79 0.1117251242119437 1101 80-81 0.09236211799014882 1101 82-83 0.14646978906844765 1101 84-85 0.040546773099407574 1101 86-87 -0.03127165979812219 1101 88-89 -0.08055625982082404 1101 90-91 -0.11025116733575402 1101 92-93 -0.15339643647237722 1101 94-95 -0.21095576820892603 1101 96-97 -0.3833395549094796 1101 98-99 -0.39631346108974697 1101 100-101 -0.1921276606114546 1102 1 0.2351985044070588 1102 2 0.20821494764052773 1102 3 0.09888181824611308 1102 4 0.14791348717815112 1102 5 0.21438104454171736 1102 6 0.23661893195642847 1102 7 0.23585204801091209 1102 8 0.23864553005751077 1102 9 0.34247551943791876 1102 10-11 0.34708893619987435 1102 12-13 0.34406453490539235 1102 14-15 0.41481202685915264 1102 16-17 0.3899970540421833 1102 18-19 0.37315817832531906 1102 20-21 0.3445844657192936 1102 22-23 0.36079050939930823 1102 24-25 0.397359331564644 1102 26-27 0.3932515628733242 1102 28-29 0.4195983646998158 1102 30-31 0.42375489673164424 1102 32-33 0.36188247635951143 1102 34-35 0.39012271598662096 1102 36-37 0.33634548123914243 1102 38-39 0.28364317853189647 1102 40-41 0.26961409713527473 1102 42-43 0.2325878484956192 1102 44-45 0.2518910992187955 1102 46-47 0.17364170060838546 1102 48-49 0.21021962896029578 1102 50-51 0.21762441719683068 1102 52-53 0.27117253008565 1102 54-55 0.3559506681250184 1102 56-57 0.28478522491317904 1102 58-59 0.2632515845270973 1102 60-61 0.26761653585568723 1102 62-63 0.26741126611644717 1102 64-65 0.29545631888431245 1102 66-67 0.28554738465105345 1102 68-69 0.17218237893648336 1102 70-71 0.14578963689728397 1102 72-73 0.1685515683548786 1102 74-75 0.12311516339876505 1102 76-77 0.026122710212284517 1102 78-79 0.03756847956133669 1102 80-81 0.08917642507437051 1102 82-83 0.07697047837579163 1102 84-85 0.007499329687092882 1102 86-87 -0.04589289859524115 1102 88-89 -0.12104863015989054 1102 90-91 -0.15635188283861368 1102 92-93 -0.21931795475036608 1102 94-95 -0.2727702850649081 1102 96-97 -0.4200050429266895 1102 98-99 -0.4683821014430194 1102 100-101 -0.2576811653898723 1103 1 0.15031441073384855 1103 2 0.16401447014747106 1103 3 0.12937036596233753 1103 4 0.14255324864350172 1103 5 0.16331743264880316 1103 6 0.1836253052062915 1103 7 0.18506921232010853 1103 8 0.15802417385213374 1103 9 0.23794314109115788 1103 10-11 0.2397436617498485 1103 12-13 0.2470285634055287 1103 14-15 0.30521412547944493 1103 16-17 0.3068584404540502 1103 18-19 0.23806995874210202 1103 20-21 0.24512988675176928 1103 22-23 0.23114330144527884 1103 24-25 0.26638923657140623 1103 26-27 0.28395985853280337 1103 28-29 0.3012622521039461 1103 30-31 0.31011571937943216 1103 32-33 0.26214648000933494 1103 34-35 0.24457838108267538 1103 36-37 0.20768463590967912 1103 38-39 0.10972216516297095 1103 40-41 0.10268554706252786 1103 42-43 0.08358841895034885 1103 44-45 0.08444085805438561 1103 46-47 0.0782419980611948 1103 48-49 0.05968032899895093 1103 50-51 0.09764127094599928 1103 52-53 0.16118136445192022 1103 54-55 0.21500791662926844 1103 56-57 0.17653100546032618 1103 58-59 0.16239318629015287 1103 60-61 0.11004576783553688 1103 62-63 0.15490101347449325 1103 64-65 0.15637433561775538 1103 66-67 0.10896641470770874 1103 68-69 0.057400824836861375 1103 70-71 0.02057910634159299 1103 72-73 0.030706525239438065 1103 74-75 -0.019111597913990863 1103 76-77 -0.09807030712787679 1103 78-79 -0.06759082725544374 1103 80-81 -0.07172704167271604 1103 82-83 -0.07794122672257942 1103 84-85 -0.1546566835221057 1103 86-87 -0.21105889816574575 1103 88-89 -0.23076756248150332 1103 90-91 -0.33725277271243925 1103 92-93 -0.3882214321737436 1103 94-95 -0.45939398338335025 1103 96-97 -0.6409784250890418 1103 98-99 -0.62582582498991 1103 100-101 -0.47471310907014086 1104 1 -0.030015014567759124 1104 2 -0.08740433098975586 1104 3 -0.17181514569951162 1104 4 -0.09729547116847215 1104 5 0.020978938107489853 1104 6 0.13117734222568345 1104 7 0.17902344815919236 1104 8 0.18391884750499088 1104 9 0.2897343337205811 1104 10-11 0.2933143177704878 1104 12-13 0.3038363325570188 1104 14-15 0.34997638507412887 1104 16-17 0.3652197874518279 1104 18-19 0.2922494960340529 1104 20-21 0.2528186476758947 1104 22-23 0.2282397179330573 1104 24-25 0.2296956812596136 1104 26-27 0.2772355412154184 1104 28-29 0.28315001913995275 1104 30-31 0.2838766277214759 1104 32-33 0.25343318008791016 1104 34-35 0.2029499631350049 1104 36-37 0.21457883474902673 1104 38-39 0.18092660227389246 1104 40-41 0.0721055046271104 1104 42-43 0.11339684491296964 1104 44-45 0.18647593795886763 1104 46-47 0.12676694764301288 1104 48-49 0.08798353212216625 1104 50-51 0.1135752607253977 1104 52-53 0.15512852262435928 1104 54-55 0.23541438763069777 1104 56-57 0.11274570231821457 1104 58-59 0.11181634045864541 1104 60-61 0.08516603025794467 1104 62-63 0.06789745464020314 1104 64-65 0.14994077800375294 1104 66-67 0.15157148113652852 1104 68-69 -0.04465259684977241 1104 70-71 -0.010367350047125967 1104 72-73 0.0526970713359205 1104 74-75 -0.005085067302260882 1104 76-77 -0.08821632656921707 1104 78-79 -0.07439175078457083 1104 80-81 -0.1324239485726153 1104 82-83 -0.08375801017000128 1104 84-85 -0.17773685218294588 1104 86-87 -0.28630002136192445 1104 88-89 -0.3394607156276308 1104 90-91 -0.42065960061968966 1104 92-93 -0.39973651831426693 1104 94-95 -0.3896243133088184 1104 96-97 -0.6489942567116138 1104 98-99 -0.6903848312223886 1104 100-101 -0.6316444368908236 1105 1 0.028780629155512827 1105 2 0.08511245010954838 1105 3 0.14228928230007654 1105 4 0.08849282306467643 1105 5 0.13295992269573986 1105 6 0.106642285196628 1105 7 0.1320364170435866 1105 8 0.1076993832005968 1105 9 0.13610942281688665 1105 10-11 0.18116379451014097 1105 12-13 0.19338590846502512 1105 14-15 0.18566668556346144 1105 16-17 0.15096241537371924 1105 18-19 0.12013379365532728 1105 20-21 -0.004863558206459118 1105 22-23 0.009053572373751706 1105 24-25 0.06896866707201355 1105 26-27 0.08814570248130593 1105 28-29 0.08136331486057458 1105 30-31 0.044360265670853494 1105 32-33 0.07149543866108132 1105 34-35 0.11482945888703 1105 36-37 0.021445524324356313 1105 38-39 -0.020232737118568878 1105 40-41 -0.06820156826778856 1105 42-43 -0.02713861520989269 1105 44-45 -0.0547522656266608 1105 46-47 -0.0947540354725831 1105 48-49 -0.06637022038788132 1105 50-51 -0.036638725756823476 1105 52-53 -0.0017660170564397504 1105 54-55 0.04225773137935107 1105 56-57 0.010241544355721999 1105 58-59 0.005806480518899093 1105 60-61 -0.044536480526375044 1105 62-63 -0.028649108812075497 1105 64-65 0.028070169990776606 1105 66-67 -0.0014683557386945267 1105 68-69 -0.03180079144939896 1105 70-71 -0.020259444040867436 1105 72-73 -0.0859051550119787 1105 74-75 -0.0825409208235186 1105 76-77 -0.15037929638878467 1105 78-79 -0.09057705434295116 1105 80-81 -0.10031083735222524 1105 82-83 -0.05377689577494493 1105 84-85 -0.14203918818076744 1105 86-87 -0.21331875560092328 1105 88-89 -0.26269587996402777 1105 90-91 -0.2480281038475738 1105 92-93 -0.31312701605024884 1105 94-95 -0.31754636759983157 1105 96-97 -0.5385162710246583 1105 98-99 -0.5665838136826338 1105 100-101 -0.4599602310051978 1106 1 -0.040341407935130746 1106 2 0.008555551544077389 1106 3 0.11691710588260662 1106 4 0.06282839758202385 1106 5 0.05512872031418681 1106 6 -0.005016553647777755 1106 7 0.07324445123690992 1106 8 0.022807437501157324 1106 9 -0.03315439019338129 1106 10-11 0.04862239537506241 1106 12-13 0.041694590492973305 1106 14-15 0.03398757702044719 1106 16-17 0.012459180811390524 1106 18-19 -0.01749633292806152 1106 20-21 -0.030876827048942346 1106 22-23 -0.036313175068457326 1106 24-25 -0.008917219482796668 1106 26-27 -0.060941719116911486 1106 28-29 -0.07049719347902084 1106 30-31 -0.07266183691442762 1106 32-33 -0.07367247237522889 1106 34-35 -0.0996230869093182 1106 36-37 -0.16717232217024502 1106 38-39 -0.1848667009370999 1106 40-41 -0.2088841099138392 1106 42-43 -0.22809728034476962 1106 44-45 -0.2866094113163413 1106 46-47 -0.3093229001846396 1106 48-49 -0.29775892762161504 1106 50-51 -0.23151060558429037 1106 52-53 -0.28158297868756677 1106 54-55 -0.20176043607202132 1106 56-57 -0.21909410532438756 1106 58-59 -0.23507873686229175 1106 60-61 -0.24446149967767639 1106 62-63 -0.23495753795529595 1106 64-65 -0.22314295964198294 1106 66-67 -0.3027365005815881 1106 68-69 -0.2981694615023258 1106 70-71 -0.2532031531603707 1106 72-73 -0.29844568060716625 1106 74-75 -0.26432028332506263 1106 76-77 -0.3808619653573473 1106 78-79 -0.38958072272592403 1106 80-81 -0.3754873029432062 1106 82-83 -0.3175796721628146 1106 84-85 -0.3391880694632263 1106 86-87 -0.4278338508630668 1106 88-89 -0.5082292692891244 1106 90-91 -0.5475937444070453 1106 92-93 -0.6219811824291952 1106 94-95 -0.6264399666960188 1106 96-97 -0.8561275729115927 1106 98-99 -0.8451999447622853 1106 100-101 -0.7350554970061332 1107 1 -0.12058948632648736 1107 2 -0.0710945471224278 1107 3 0.06198207614461637 1107 4 0.05008823023475628 1107 5 0.008620143028053917 1107 6 -0.03727024909264287 1107 7 -0.03376265143596413 1107 8 -0.05400755565314341 1107 9 -0.06085939538112939 1107 10-11 -0.017480996374317215 1107 12-13 -0.01998218450155065 1107 14-15 -0.06076271037184 1107 16-17 -0.10751884757180363 1107 18-19 -0.15894444812322206 1107 20-21 -0.15760252537420172 1107 22-23 -0.15132625134668132 1107 24-25 -0.09622604491926268 1107 26-27 -0.16330142981873763 1107 28-29 -0.1350096665013396 1107 30-31 -0.2089514160560242 1107 32-33 -0.13302697527988272 1107 34-35 -0.16590487901288498 1107 36-37 -0.2228076891141555 1107 38-39 -0.27867756931582477 1107 40-41 -0.285981141198107 1107 42-43 -0.33622240349728827 1107 44-45 -0.32613974729612494 1107 46-47 -0.288484957419854 1107 48-49 -0.31862688074863854 1107 50-51 -0.25171060075393115 1107 52-53 -0.3343889328792997 1107 54-55 -0.34388060767305717 1107 56-57 -0.33440942594928913 1107 58-59 -0.28613152749035464 1107 60-61 -0.2985321284278868 1107 62-63 -0.2986470425661949 1107 64-65 -0.32255029072922525 1107 66-67 -0.34344764323030574 1107 68-69 -0.2971055638765563 1107 70-71 -0.27165425069046734 1107 72-73 -0.32272155011299475 1107 74-75 -0.3522734712377158 1107 76-77 -0.4902868710875836 1107 78-79 -0.36889167167430514 1107 80-81 -0.3694703378663249 1107 82-83 -0.3096350746113785 1107 84-85 -0.3968961820436334 1107 86-87 -0.4146986494389324 1107 88-89 -0.4414407834385372 1107 90-91 -0.40383239751283995 1107 92-93 -0.48662503622357534 1107 94-95 -0.4715503538548447 1107 96-97 -0.6094825299452289 1107 98-99 -0.6589838257702993 1107 100-101 -0.4620318795776406 1108 1 -0.27397706889743034 1108 2 -0.16662134181100896 1108 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-1.1708696629356083 2308 88-89 -1.4239044973685857 2308 90-91 -1.420079546816055 2308 92-93 -1.469947130822682 2308 94-95 -1.8682258718500186 2308 96-97 -1.7055673365645028 2308 98-99 -1.4301344701333107 2308 100-101 -1.8050258553396539 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 8.0 11 117.0 12 578.0 13 1915.0 14 5007.0 15 10460.0 16 16902.0 17 26488.0 18 42886.0 19 72194.0 20 80349.0 21 80679.0 22 83282.0 23 88851.0 24 100886.0 25 117650.0 26 142396.0 27 175590.0 28 223158.0 29 281025.0 30 351935.0 31 455769.0 32 604418.0 33 829966.0 34 1216091.0 35 1937750.0 36 3252288.0 37 6051547.0 38 6136565.0 39 951582.0 40 434.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.47525523639926 30.02737218450791 24.628305292070042 21.86906728702279 2 23.793269007254946 28.506969253614244 27.05629242434987 20.643469314780937 3 23.144103493754447 27.054079691719714 26.270259162429404 23.531557652096442 4 24.574157005558906 26.60954310952002 26.271573227136347 22.544726657784732 5 24.38920292529605 27.640077457394273 25.1487803596814 22.821939257628273 6 24.510550386425745 26.581499638841233 24.57691207838495 24.331037896348075 7 24.833965086243207 26.901246621179542 25.34717988089002 22.917608411687233 8 24.470569695072996 26.334318618216574 25.429142226285656 23.76596946042477 9 23.85620987844859 25.913538016534375 25.800472912749544 24.429779192267493 10-11 23.916710077987844 26.379717333812764 25.67680527753695 24.02676731066244 12-13 23.914497021821976 26.333382407621723 25.927889675058225 23.824230895498076 14-15 23.556892425246474 26.42218316084064 26.061343603170794 23.959580810742093 16-17 23.737649625520046 26.70754314945357 26.01802083280667 23.536786392219707 18-19 23.1544225603016 26.57425846764992 26.725054786529846 23.546264185518634 20-21 22.850109127449155 26.505724424333316 26.78846002397899 23.85570642423854 22-23 22.878735319596586 26.404737936872923 27.435227295222038 23.28129944830845 24-25 22.88675416686555 26.054655160431363 28.07667080598863 22.981919866714463 26-27 22.914874762444594 26.035725282133598 28.163935488277314 22.88546446714449 28-29 22.910407945304392 26.123062804605862 28.128068124938565 22.838461125151174 30-31 22.937836559139416 26.073741430887992 28.089276442464868 22.899145567507727 32-33 22.937465754398993 26.094413140301686 28.077950057680862 22.89017104761846 34-35 22.956168719460145 26.165481499750243 28.03422640254416 22.84412337824545 36-37 23.009481306766606 26.17089095456032 27.903506123674234 22.91612161499884 38-39 23.021988394759173 26.21094448609665 27.805234004231416 22.961833114912757 40-41 23.120170535151686 26.401376576636487 27.466304345311144 23.012148542900682 42-43 23.136368906565156 26.454007893990628 27.36424239396376 23.045380805480463 44-45 23.106247348295962 26.56166782768206 27.33796422655765 22.99412059746432 46-47 23.114345462823525 26.7208493371072 27.184267154484516 22.98053804558476 48-49 23.142678151878297 26.69629362580695 27.1278824253176 23.03314579699715 50-51 23.088977797712186 26.753663839810553 27.149518102199576 23.007840260277685 52-53 23.134612668352144 26.77092753684851 27.087754491556122 23.006705303243223 54-55 23.128943492556548 26.807644842919288 27.031373466789116 23.032038197735048 56-57 23.13385806258994 26.83307463642251 27.015260789709277 23.017806511278273 58-59 23.125370038844384 26.92444621965017 26.974466002187093 22.975717739318352 60-61 23.113700612962997 26.886715004555086 26.902176404699375 23.097407977782545 62-63 23.130635955645644 26.974650245004383 26.84768552030557 23.0470282790444 64-65 23.129567886520793 27.058226128108444 26.795930232732523 23.016275752638247 66-67 23.107667117476648 27.04958864089103 26.756957061763252 23.08578717986907 68-69 23.103965800131103 27.12458934393107 26.684731613150745 23.08671324278708 70-71 23.09138305619727 27.23598433870732 26.624602459954204 23.0480301451412 72-73 23.0742223841466 27.21091938935352 26.59810141429651 23.116756812203366 74-75 23.06299906250375 27.283081877529053 26.58603756154997 23.067881498417226 76-77 23.0753935978319 27.382474450073207 26.480342330017415 23.061789622077484 78-79 23.07609529641271 27.358238978522742 26.432360462548914 23.133305262515634 80-81 23.04329669661993 27.431764690809896 26.42734049391113 23.09759811865904 82-83 23.048741255942666 27.531278678567045 26.38163797021067 23.03834209527962 84-85 23.00166455069182 27.52956292974033 26.365431487372458 23.103341032195388 86-87 23.01215499601606 27.631929280085505 26.30261022071651 23.053305503181925 88-89 23.01030733740564 27.714658499258977 26.241632055575533 23.033402107759855 90-91 23.011455709816104 27.737098829491767 26.152283043784152 23.09916241690797 92-93 22.9896109702368 27.799163278163324 26.13322025300987 23.078005498590002 94-95 23.00352409164065 27.89718958121062 26.065423764130415 23.033862563018314 96-97 22.94970786909997 27.93362836683559 26.031540877726776 23.08512288633766 98-99 22.947885743505246 28.00729709751802 25.9954707212553 23.049346437721432 100-101 22.956908891533274 28.152303374463433 25.640628315636903 23.25015941836639 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 122991.0 1 68965.0 2 11279.5 3 6557.5 4 5114.5 5 4643.0 6 4845.0 7 5225.0 8 5755.5 9 5846.5 10 5780.5 11 6378.5 12 8517.5 13 13267.0 14 18142.5 15 19641.0 16 48411.0 17 51866.5 18 24962.0 19 23879.0 20 25056.5 21 28455.0 22 34170.5 23 41914.0 24 51406.5 25 63486.5 26 78187.0 27 95587.5 28 115672.5 29 139711.0 30 172200.0 31 210513.0 32 252748.0 33 303607.0 34 353380.0 35 403550.5 36 462271.0 37 519109.5 38 562719.0 39 601959.0 40 650505.5 41 699267.5 42 748428.5 43 811438.0 44 874562.5 45 919711.0 46 941439.5 47 953214.5 48 965226.0 49 970360.5 50 969002.0 51 962872.0 52 948925.0 53 927497.0 54 882362.5 55 816665.0 56 742562.5 57 653698.5 58 569521.0 59 488438.5 60 401454.0 61 329341.5 62 271214.0 63 213087.0 64 165233.0 65 127684.5 66 94842.0 67 70277.0 68 53558.0 69 42438.5 70 35011.0 71 29145.5 72 24456.0 73 19133.5 74 14375.5 75 11528.0 76 8936.5 77 6057.5 78 3815.0 79 2332.5 80 1242.0 81 736.5 82 473.0 83 259.0 84 104.0 85 28.5 86 13.0 87 4.5 88 4.0 89 3.0 90 1.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09908835797059706 2 0.0038605297298066233 3 2.142358340625207E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 8.569433362500828E-6 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.142358340625207E-6 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0018381434562564276 66-67 1.456803671625141E-4 68-69 8.440891862063316E-4 70-71 3.406349761594079E-4 72-73 1.0711791703126035E-4 74-75 2.5708300087502484E-5 76-77 2.142358340625207E-6 78-79 7.498254192188225E-4 80-81 0.002223767957568965 82-83 0.0014739425383501425 84-85 1.521074421843897E-4 86-87 2.142358340625207E-4 88-89 6.384227855063117E-4 90-91 7.96957302712577E-4 92-93 0.0033549331614190743 94-95 0.0017631609143345453 96-97 0.0021959172991408375 98-99 7.348289108344459E-4 100-101 7.926725860313265E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.3338766E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.25254743905242 #Duplication Level Percentage of deduplicated Percentage of total 1 74.42304711326577 38.887937998447526 2 13.629343660896751 14.243358524083119 3 4.982807187387241 7.810931068158096 4 2.358663086539533 4.929846192885949 5 1.2866215747184784 3.361462743454281 6 0.8014718313790734 2.5127366934119557 7 0.5528035772464374 2.0219776601183144 8 0.37652716057637753 1.57396026560871 9 0.2453128145102046 1.153639752984174 >10 1.182091458625345 10.79981388858391 >50 0.07976857244225535 2.975951459562665 >100 0.0810627779869369 7.556998927477138 >500 3.16157805052242E-4 0.09926829253963612 >1k 4.9048008753648165E-5 0.03813940483185709 >5k 3.2565317663455854E-5 0.12569872894468154 >10k+ 8.141329415797828E-5 1.9082783989081389 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 168022 0.719926666217057 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 103464 0.4433139267088928 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45238 0.19383201322640622 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34275 0.14685866424985794 No Hit GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33603 0.14397933464005766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00527877095130051 0.0 0.0 0.0 0.0 2 0.005304479251388013 0.0 0.0 4.284716681250414E-6 8.569433362500828E-6 3 0.005343041701519266 0.0 0.0 4.284716681250414E-6 8.569433362500828E-6 4 0.005351611134881767 0.0 0.0 8.569433362500828E-6 1.7138866725001656E-5 5 0.0053901735850130205 0.0 0.0 1.2854150043751242E-5 2.5708300087502484E-5 6 0.005428736035144275 0.0 0.0 1.7138866725001656E-5 2.5708300087502484E-5 7 0.005467298485275528 0.0 0.0 1.7138866725001656E-5 2.5708300087502484E-5 8 0.0055229998021317835 0.0 0.0 1.7138866725001656E-5 2.99930167687529E-5 9 0.005625833002481794 0.0 0.0 1.7138866725001656E-5 2.99930167687529E-5 10-11 0.0057500897862380555 0.0 0.0 1.7138866725001656E-5 2.99930167687529E-5 12-13 0.005960040903619327 0.0 0.0 1.7138866725001656E-5 3.2135375109378105E-5 14-15 0.006221408621175602 0.0 0.0 2.142358340625207E-5 5.141660017500497E-5 16-17 0.0065191964305225045 0.0 0.0 2.142358340625207E-5 5.99860335375058E-5 18-19 0.006864116123363163 0.0 0.0 2.5708300087502484E-5 7.069782524063183E-5 20-21 0.007213320532885072 0.0 0.0 2.5708300087502484E-5 7.284018358125704E-5 22-23 0.007631080409306988 2.142358340625207E-6 0.0 2.7850658428127693E-5 7.498254192188224E-5 24-25 0.008038128494025777 4.284716681250414E-6 0.0 2.99930167687529E-5 8.140961694375786E-5 26-27 0.008500877895600821 4.284716681250414E-6 0.0 3.427773345000331E-5 8.569433362500829E-5 28-29 0.008987193238922744 4.284716681250414E-6 0.0 3.427773345000331E-5 9.426376698750911E-5 30-31 0.009499216882332167 4.284716681250414E-6 0.0 3.427773345000331E-5 1.0497555869063514E-4 32-33 0.009946969775522837 4.284716681250414E-6 0.0 3.427773345000331E-5 1.1354499205313597E-4 34-35 0.010568253694304147 4.284716681250414E-6 0.0 3.642009179062852E-5 1.3068385877813761E-4 36-37 0.011735838989944885 6.427075021875621E-6 0.0 3.8562450131253724E-5 1.4568036716251407E-4 38-39 0.012346411117023068 8.569433362500828E-6 0.0 3.8562450131253724E-5 1.4782272550313926E-4 40-41 0.012931274944013749 8.569433362500828E-6 0.0 4.284716681250414E-5 1.563921588656401E-4 42-43 0.013678958004891946 8.569433362500828E-6 0.0 4.927424183437976E-5 1.6496159222814094E-4 44-45 0.014585175582976409 8.569433362500828E-6 0.0 5.141660017500497E-5 1.7567338393126698E-4 46-47 0.015574945136345254 8.569433362500828E-6 0.0 5.784367519688059E-5 1.86385175634393E-4 48-49 0.016611846573207856 8.569433362500828E-6 0.0 6.855546690000662E-5 2.0566640070001987E-4 50-51 0.017687310460201708 8.569433362500828E-6 0.0 6.855546690000662E-5 2.163781924031459E-4 52-53 0.019223381390429985 8.569433362500828E-6 0.0 6.855546690000662E-5 2.206629090843963E-4 54-55 0.021108656730180163 8.569433362500828E-6 0.0 6.855546690000662E-5 2.2280526742502152E-4 56-57 0.023049633386786603 8.569433362500828E-6 0.0 6.855546690000662E-5 2.3137470078752237E-4 58-59 0.025136290410555553 8.569433362500828E-6 0.0 6.855546690000662E-5 2.37801775809398E-4 60-61 0.027835661919743314 8.569433362500828E-6 0.0 7.284018358125704E-5 2.48513567512524E-4 62-63 0.031036345280637374 8.569433362500828E-6 0.0 7.926725860313265E-5 2.527982841937744E-4 64-65 0.03508540254441902 8.569433362500828E-6 0.0 8.569433362500827E-5 2.6565243423752567E-4 66-67 0.03983929570226635 8.569433362500828E-6 0.0 9.854848366875952E-5 2.742218676000265E-4 68-69 0.04540514267121064 8.569433362500828E-6 0.0 1.0283320035000993E-4 2.7850658428127693E-4 70-71 0.056131930882721046 8.569433362500828E-6 0.0 1.0711791703126035E-4 2.8493365930315256E-4 72-73 0.06764710696358153 8.569433362500828E-6 0.0 1.1140263371251076E-4 2.9136073432502814E-4 74-75 0.07771404880617938 8.569433362500828E-6 0.0 1.1140263371251076E-4 2.956454510062786E-4 76-77 0.0901654354818931 8.569433362500828E-6 0.0 1.1568735039376118E-4 2.956454510062786E-4 78-79 0.10488986435701014 8.569433362500828E-6 0.0 1.1782970873438639E-4 2.956454510062786E-4 80-81 0.12249362284192745 8.569433362500828E-6 2.142358340625207E-6 1.2854150043751242E-4 2.956454510062786E-4 82-83 0.1431502419622357 8.569433362500828E-6 4.284716681250414E-6 1.3282621711876283E-4 2.9993016768752897E-4 84-85 0.16810014719715688 8.569433362500828E-6 4.284716681250414E-6 1.3925329214063844E-4 3.042148843687794E-4 86-87 0.19763041456433472 8.569433362500828E-6 4.284716681250414E-6 1.4353800882188888E-4 3.084996010500298E-4 88-89 0.23271581710875372 8.569433362500828E-6 4.284716681250414E-6 1.4996508384376448E-4 3.149266760719054E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 15165 0.0 85.252525 1 TCGACGG 16740 0.0 77.40939 2 CGACGGC 19790 0.0 65.61998 3 ATCTCGA 21845 0.0 58.94366 1 ACGGCGC 22665 0.0 57.442947 5 GACGGCG 22925 0.0 56.812187 4 CGGCGCG 25700 0.0 50.73324 6 TCTCGAG 26950 0.0 47.83611 2 CTCGAGG 34070 0.0 37.90709 3 TCGAGGG 34220 0.0 37.671524 4 TATCTCG 6695 0.0 35.971798 2 CGAGGGC 37845 0.0 34.226303 5 AGGGCGC 41755 0.0 30.850668 7 GGGCGCG 41745 0.0 30.823923 8 GAGGGCG 44205 0.0 29.280499 6 CGCGCCG 67215 0.0 27.694431 9 GGCGCGC 68550 0.0 27.1828 7 GCGCGCC 69580 0.0 26.80772 8 ATATCTC 12235 0.0 20.208767 1 CCATATT 36535 0.0 20.190437 18-19 >>END_MODULE