FastQCFastQC Report
Tue 31 May 2016
SRR1051279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22267358
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT7988323.587457479239342No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT812660.36495573475757653No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT589490.26473279856550563No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347880.15622868236096982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1159600.093.748671
TCGACGG1188100.091.56382
TATCTCG1106750.090.79242
ATATCTC1137050.088.560951
ACGGCGC1248250.087.140165
CGACGGC1248850.087.086883
CGGCGCG1257850.086.441116
GACGGCG1297700.083.859874
ATCTCGA1201900.083.462443
TCTCGAG1212950.081.139684
CTCGAGG1257200.078.3485
TCGAGGG1270050.077.446856
AGGGCGC1320650.074.648559
GAGGGCG1351100.073.0435268
CGAGGGC1364750.072.1145867
CGCGCCG2527000.044.893719
GCGCGCC2540500.044.677588
GGCGCGC2542350.044.6469357
ATCCATA1353300.040.03738816-17
TCCATAT1366850.039.70615818-19
Produced by FastQC (version 0.11.5)