##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051279_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22267358 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.502021479153477 33.0 31.0 34.0 30.0 34.0 2 31.657780999434237 33.0 31.0 34.0 30.0 34.0 3 31.95844962837531 33.0 31.0 34.0 30.0 34.0 4 35.35258143332496 37.0 35.0 37.0 33.0 37.0 5 35.39127915399752 37.0 35.0 37.0 33.0 37.0 6 35.39823521048164 37.0 35.0 37.0 33.0 37.0 7 35.40273031043916 37.0 35.0 37.0 33.0 37.0 8 35.455705656683655 37.0 35.0 37.0 33.0 37.0 9 37.08607716281384 39.0 37.0 39.0 33.0 39.0 10-11 37.1904411381 39.0 37.0 39.0 34.0 39.0 12-13 37.08208566997486 39.0 37.0 39.0 33.0 39.0 14-15 38.30345436580308 40.0 38.0 41.0 33.5 41.0 16-17 38.16809978085411 40.0 38.0 41.0 32.5 41.0 18-19 38.1593850289738 40.0 38.0 41.0 33.0 41.0 20-21 38.23653870387318 40.0 38.0 41.0 33.5 41.0 22-23 38.2571598076431 40.0 38.0 41.0 33.5 41.0 24-25 38.2654381359477 40.0 38.0 41.0 34.0 41.0 26-27 38.287751919199394 40.0 38.0 41.0 34.0 41.0 28-29 38.068575625361575 40.0 38.0 41.0 33.0 41.0 30-31 37.626756932726366 39.5 36.5 41.0 33.0 41.0 32-33 37.338346718097405 39.0 35.5 41.0 33.0 41.0 34-35 37.20401196675421 39.0 35.0 41.0 32.0 41.0 36-37 37.2072546954156 39.0 35.0 41.0 32.0 41.0 38-39 37.15119972921799 39.0 35.0 41.0 31.5 41.0 40-41 36.61773376976289 39.0 35.0 41.0 30.0 41.0 42-43 36.56902585838877 39.0 35.0 41.0 30.0 41.0 44-45 36.46879499579609 39.0 35.0 41.0 30.0 41.0 46-47 36.4860278439858 39.0 35.0 41.0 30.0 41.0 48-49 36.42271970478042 39.0 35.0 41.0 29.5 41.0 50-51 36.24711640689479 38.5 35.0 40.5 29.0 41.0 52-53 36.11465044483499 39.0 35.0 40.0 29.0 41.0 54-55 35.802742202285515 38.5 35.0 40.0 28.0 41.0 56-57 35.52668349788062 38.0 34.5 40.0 28.0 41.0 58-59 35.13379288193956 38.0 34.0 40.0 26.5 41.0 60-61 34.864948055355285 38.0 34.0 40.0 26.0 41.0 62-63 34.5222155901926 37.5 34.0 40.0 25.5 41.0 64-65 34.26223667846001 37.0 34.0 40.0 25.0 41.0 66-67 33.8436766050108 36.5 33.5 39.5 24.5 41.0 68-69 33.38517557853069 36.0 33.0 39.0 23.0 41.0 70-71 32.982699519179604 35.5 33.0 39.0 22.5 40.5 72-73 32.526784587556364 35.0 32.5 38.0 20.5 40.0 74-75 32.13940412239297 35.0 32.0 37.0 20.0 39.0 76-77 31.66282142677187 35.0 32.0 37.0 18.0 39.0 78-79 31.40532442151422 35.0 32.0 36.0 18.0 39.0 80-81 31.04910542148736 35.0 32.0 36.0 16.5 37.0 82-83 30.72141037118099 35.0 31.0 35.5 12.5 37.0 84-85 30.4382288639721 35.0 31.0 35.0 8.5 36.5 86-87 30.175683594793778 35.0 31.0 35.0 7.0 36.0 88-89 29.902923867303883 34.5 31.0 35.0 2.0 36.0 90-91 29.67636317698759 34.0 31.0 35.0 2.0 35.5 92-93 29.4838580984776 34.0 31.0 35.0 2.0 35.0 94-95 29.263572333098523 34.0 31.0 35.0 2.0 35.0 96-97 28.9733910731574 34.0 30.0 35.0 2.0 35.0 98-99 28.627361876518982 34.0 30.0 35.0 2.0 35.0 100-101 27.392718503021328 33.0 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.16509720293844055 1101 2 -0.09939729502270822 1101 3 0.06684580469561041 1101 4 -0.06451847488045814 1101 5 0.0021481510023875217 1101 6 -0.002881395427237976 1101 7 0.08676186712143164 1101 8 0.11284212480692446 1101 9 0.19458155015423273 1101 10-11 0.2961610538420558 1101 12-13 0.41163574749652554 1101 14-15 0.3743875684657212 1101 16-17 0.30128114628299585 1101 18-19 0.21871786555405492 1101 20-21 0.4053133602874368 1101 22-23 0.5006819091749648 1101 24-25 0.6407719018589546 1101 26-27 0.5563537697818148 1101 28-29 0.6708756039342134 1101 30-31 0.6628235002996163 1101 32-33 0.6053793086807744 1101 34-35 0.4945117930181624 1101 36-37 0.567630278680916 1101 38-39 0.5040657942155562 1101 40-41 0.35727550730354096 1101 42-43 0.30760105412782934 1101 44-45 0.2523126323977323 1101 46-47 0.18904255213081456 1101 48-49 0.1774831197449842 1101 50-51 0.16268592023735806 1101 52-53 0.08559392722251857 1101 54-55 0.06222423385379017 1101 56-57 -0.028135481612167723 1101 58-59 -0.05313534580337631 1101 60-61 0.029657650995162044 1101 62-63 0.018763193280015855 1101 64-65 -0.05939425310337754 1101 66-67 -0.05121847733673235 1101 68-69 -0.04868985702086093 1101 70-71 -0.12180618922293718 1101 72-73 -0.1026057586740663 1101 74-75 -0.1397547946309743 1101 76-77 -0.047454893520992414 1101 78-79 -0.17114880545299727 1101 80-81 -0.1702641434783594 1101 82-83 -0.20102373489524084 1101 84-85 -0.1568290549401432 1101 86-87 -0.12431368212607552 1101 88-89 -0.05817123749659814 1101 90-91 -0.08891148571960272 1101 92-93 -0.033867040765574785 1101 94-95 -0.02980743967733801 1101 96-97 -0.0495755720787443 1101 98-99 -0.07601885143197507 1101 100-101 0.17429999602508417 1102 1 -0.211108081911906 1102 2 -0.109602585492663 1102 3 0.04167238692936337 1102 4 -0.1137896267246461 1102 5 -0.07667389060858909 1102 6 -0.059691765694026344 1102 7 -7.373763896865171E-4 1102 8 -0.011507700649488584 1102 9 0.04817000263663829 1102 10-11 0.1821662489690823 1102 12-13 0.2863431408854211 1102 14-15 0.22205055937980944 1102 16-17 0.16768355486504305 1102 18-19 0.16610475528383972 1102 20-21 0.27290171950356523 1102 22-23 0.37862800792107976 1102 24-25 0.4764902646664808 1102 26-27 0.4109161360060014 1102 28-29 0.47534075848298585 1102 30-31 0.47181549383709864 1102 32-33 0.4380871705871954 1102 34-35 0.36809269146889534 1102 36-37 0.40524102762476844 1102 38-39 0.30399767004274025 1102 40-41 0.1697161358986321 1102 42-43 0.10959985954606566 1102 44-45 0.08167990483725873 1102 46-47 0.07832056476018323 1102 48-49 0.08993286128769995 1102 50-51 -0.0019710537308839093 1102 52-53 -0.032117611023160464 1102 54-55 -0.05295242908783848 1102 56-57 -0.09034239995925475 1102 58-59 -0.10744951677401815 1102 60-61 -0.08214566531444234 1102 62-63 -0.09258022032594226 1102 64-65 -0.2270364390072146 1102 66-67 -0.1867824888534244 1102 68-69 -0.1632664402368107 1102 70-71 -0.2055961432638611 1102 72-73 -0.18995668729520077 1102 74-75 -0.2538465133566845 1102 76-77 -0.287737672097208 1102 78-79 -0.3608144927313077 1102 80-81 -0.26492012295586775 1102 82-83 -0.2958134443394833 1102 84-85 -0.3186504335888074 1102 86-87 -0.35026441778873973 1102 88-89 -0.28721906230667216 1102 90-91 -0.2967911982619107 1102 92-93 -0.23930428224623057 1102 94-95 -0.22597252988377292 1102 96-97 -0.2280310470393303 1102 98-99 -0.24353017975354874 1102 100-101 0.0047404988104062795 1103 1 -0.0773268486618548 1103 2 -0.04512321968281441 1103 3 0.10387152149964862 1103 4 0.01789899233070713 1103 5 0.06083889343971549 1103 6 0.027825845699034346 1103 7 0.11971095031426415 1103 8 0.10854674776494733 1103 9 0.19792251234697034 1103 10-11 0.2541075567979121 1103 12-13 0.3599307462218846 1103 14-15 0.2789452993734116 1103 16-17 0.24872707662499494 1103 18-19 0.25588951337731203 1103 20-21 0.3791941155900247 1103 22-23 0.40065130233614354 1103 24-25 0.49983730252724 1103 26-27 0.4571206345690726 1103 28-29 0.5284395648278633 1103 30-31 0.5284839039745748 1103 32-33 0.4684774566614678 1103 34-35 0.44429366309709906 1103 36-37 0.5003998520935795 1103 38-39 0.34496816824334076 1103 40-41 0.27926389645588046 1103 42-43 0.25435120698721647 1103 44-45 0.20582067660837566 1103 46-47 0.18680137322306933 1103 48-49 0.126523378073351 1103 50-51 0.10048303091281952 1103 52-53 0.0763027382184518 1103 54-55 0.044511987583433665 1103 56-57 -5.360999597101568E-4 1103 58-59 -0.024510401332541676 1103 60-61 0.05681891072448764 1103 62-63 0.036030195614458194 1103 64-65 -0.09136693152205311 1103 66-67 -0.06087446307765987 1103 68-69 -0.016324062397607975 1103 70-71 -0.09402640219065006 1103 72-73 -0.0867368071961323 1103 74-75 -0.12189619268987428 1103 76-77 0.0018377157349078743 1103 78-79 -0.1317374257300692 1103 80-81 -0.09256283061196413 1103 82-83 -0.12138994881877707 1103 84-85 -0.10122609170644381 1103 86-87 -0.07909811644571363 1103 88-89 0.001180980158370204 1103 90-91 -0.009052218940240664 1103 92-93 0.013754237133035474 1103 94-95 0.005339469652792417 1103 96-97 0.06563212321233891 1103 98-99 0.039555158166429294 1103 100-101 0.2948373722930029 1104 1 -0.2659674238970524 1104 2 -0.18913879006601064 1104 3 -0.03922622410563292 1104 4 -0.197477288873948 1104 5 -0.12630627620192314 1104 6 -0.05799882955952995 1104 7 0.013326693724771133 1104 8 0.026091778304760282 1104 9 0.00292332874003165 1104 10-11 0.049130749704261234 1104 12-13 0.1302582627263078 1104 14-15 0.034760006060679416 1104 16-17 4.6004102150476456E-4 1104 18-19 0.06494965957828214 1104 20-21 0.1680747273785883 1104 22-23 0.24170516880734283 1104 24-25 0.27925793519297315 1104 26-27 0.26564143202633517 1104 28-29 0.2384300037285243 1104 30-31 0.26916569418630587 1104 32-33 0.19617940994063332 1104 34-35 0.004900662191822391 1104 36-37 0.17458573840065128 1104 38-39 0.14960431340783487 1104 40-41 0.02912166369671354 1104 42-43 -0.056361079949695636 1104 44-45 -0.05326018717395442 1104 46-47 -0.13236221517593805 1104 48-49 -0.080391111615576 1104 50-51 -0.12766185408116115 1104 52-53 -0.2698287337055518 1104 54-55 -0.25158608588787956 1104 56-57 -0.2619467767701025 1104 58-59 -0.1440992127689853 1104 60-61 -0.1551460583661992 1104 62-63 -0.16961337758129957 1104 64-65 -0.14850331906919934 1104 66-67 -0.21247096921739228 1104 68-69 -0.1799180466044561 1104 70-71 -0.3624090101539963 1104 72-73 -0.30127359275331855 1104 74-75 -0.32077601031085123 1104 76-77 -0.14792255539026655 1104 78-79 -0.16814084890817327 1104 80-81 -0.1881111687098702 1104 82-83 -0.2798181411356886 1104 84-85 -0.3281692733883439 1104 86-87 -0.23037318377720695 1104 88-89 -0.13724332878852508 1104 90-91 -0.17774505809751062 1104 92-93 -0.15843627647019787 1104 94-95 -0.1151996203175436 1104 96-97 -0.12826887527730335 1104 98-99 -0.14710402001834666 1104 100-101 0.12920684137263905 1105 1 0.1338009026073479 1105 2 0.06081545025056556 1105 3 0.11564573964047753 1105 4 -0.10838005526649397 1105 5 0.1187105170284184 1105 6 0.10951362125303632 1105 7 0.045342452726728766 1105 8 0.0176944115249853 1105 9 0.07368745838145685 1105 10-11 0.021303433988236975 1105 12-13 0.10956642676502071 1105 14-15 0.00997448304265447 1105 16-17 0.09140112083152019 1105 18-19 0.07631400030151525 1105 20-21 0.106126051863356 1105 22-23 0.11499022362325206 1105 24-25 0.22429146339818828 1105 26-27 0.1424391889980683 1105 28-29 0.15668350237161377 1105 30-31 0.11250830491993469 1105 32-33 0.1050270698416611 1105 34-35 0.018113299875182065 1105 36-37 -0.02672892551279915 1105 38-39 -0.018704190921475572 1105 40-41 0.0026703101258007678 1105 42-43 -0.06551522635463414 1105 44-45 -0.11562591082637397 1105 46-47 -0.21657017336881523 1105 48-49 -0.19718446659241096 1105 50-51 -0.1521289777006274 1105 52-53 -0.16613008555855657 1105 54-55 -0.24754887239680556 1105 56-57 -0.39261456987176757 1105 58-59 -0.3660150968746123 1105 60-61 -0.30217581285305073 1105 62-63 -0.30791061269933095 1105 64-65 -0.31866376721588807 1105 66-67 -0.3281254376513374 1105 68-69 -0.34898591579657534 1105 70-71 -0.3769455495364582 1105 72-73 -0.4200719475004888 1105 74-75 -0.4904891192533185 1105 76-77 -0.37307403336563283 1105 78-79 -0.35241476421944995 1105 80-81 -0.3968510724690155 1105 82-83 -0.4291369011895547 1105 84-85 -0.39891409534406463 1105 86-87 -0.43215083536887633 1105 88-89 -0.34503124075921576 1105 90-91 -0.31364522511661974 1105 92-93 -0.3050730073144763 1105 94-95 -0.32677107730696875 1105 96-97 -0.3417086089364325 1105 98-99 -0.3361825765335311 1105 100-101 -0.13156095188210415 1106 1 -0.09353185794259034 1106 2 -0.19983320013281514 1106 3 -0.052107705011231076 1106 4 -0.11526713186071902 1106 5 -0.09752967021053394 1106 6 -0.07543799297977927 1106 7 -0.10113816162056821 1106 8 -0.1672990097906606 1106 9 -0.3158997394324885 1106 10-11 -0.3100244779247845 1106 12-13 -0.18672785901758715 1106 14-15 -0.28673406560449166 1106 16-17 -0.1693977484655207 1106 18-19 -0.1469495691458036 1106 20-21 -0.22989279701261012 1106 22-23 -0.17389132963160137 1106 24-25 -0.09960816718635357 1106 26-27 -0.17383864595028342 1106 28-29 -0.10362872915796117 1106 30-31 -0.07929985090986946 1106 32-33 -0.13568345974259444 1106 34-35 -0.25673123017016763 1106 36-37 -0.3234375439085042 1106 38-39 -0.2441763157569241 1106 40-41 -0.3318168090462237 1106 42-43 -0.3562521923875437 1106 44-45 -0.3733685884131077 1106 46-47 -0.45789626324425825 1106 48-49 -0.3978354733078717 1106 50-51 -0.43612067263268983 1106 52-53 -0.35481764867690657 1106 54-55 -0.47833564993955946 1106 56-57 -0.48983895190607285 1106 58-59 -0.6358156499183494 1106 60-61 -0.4816628370234355 1106 62-63 -0.4408527091172516 1106 64-65 -0.5880284712409036 1106 66-67 -0.5322700318727485 1106 68-69 -0.5837387878618259 1106 70-71 -0.6091684930477044 1106 72-73 -0.6043594164402251 1106 74-75 -0.654836729834166 1106 76-77 -0.6158806985291747 1106 78-79 -0.6204958480221663 1106 80-81 -0.6109653055927708 1106 82-83 -0.7024455699543921 1106 84-85 -0.6463730449715896 1106 86-87 -0.63690053170005 1106 88-89 -0.6645274461584556 1106 90-91 -0.6978624473051447 1106 92-93 -0.6117303785082449 1106 94-95 -0.6378690501823598 1106 96-97 -0.7755848629212068 1106 98-99 -0.7583890232380135 1106 100-101 -0.4754374783375859 1107 1 -0.019872373768730967 1107 2 -0.22583235162174375 1107 3 -0.21244635636874776 1107 4 -0.2339469495768185 1107 5 -0.09936229935884455 1107 6 -0.15198551976359198 1107 7 -0.19078039740155361 1107 8 -0.25476429396659483 1107 9 -0.2769722153212797 1107 10-11 -0.3035094945172929 1107 12-13 -0.22811047588098887 1107 14-15 -0.3284176133786758 1107 16-17 -0.28544439898867324 1107 18-19 -0.25654476973288354 1107 20-21 -0.3293438043769896 1107 22-23 -0.3688979091580151 1107 24-25 -0.3262430542751673 1107 26-27 -0.4192239189624942 1107 28-29 -0.3288294794233053 1107 30-31 -0.32454316874750333 1107 32-33 -0.4269072072910163 1107 34-35 -0.5120000532505742 1107 36-37 -0.3828974109667982 1107 38-39 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-0.9956241445590734 2308 12-13 -1.1359765759760947 2308 14-15 -1.397643455174581 2308 16-17 -1.222414318987326 2308 18-19 -1.0981161108829838 2308 20-21 -1.111714855325431 2308 22-23 -1.1694890517189407 2308 24-25 -1.599580931944942 2308 26-27 -1.158236774794382 2308 28-29 -0.8631376078745134 2308 30-31 -1.3263084854824783 2308 32-33 -1.1884413010402355 2308 34-35 -1.9655116188782813 2308 36-37 -2.1296782018698366 2308 38-39 -1.2063983076547942 2308 40-41 -2.748513124083111 2308 42-43 -1.4191668461882614 2308 44-45 -1.7251542013476069 2308 46-47 -1.605456520683326 2308 48-49 -1.7251946738485984 2308 50-51 -2.4700798358268443 2308 52-53 -2.379883127844529 2308 54-55 -1.8440300539455166 2308 56-57 -1.6306434458231038 2308 58-59 -2.397566538553555 2308 60-61 -2.022852572532244 2308 62-63 -1.8393453610989638 2308 64-65 -1.7634480847070364 2308 66-67 -2.2441840229998533 2308 68-69 -2.7802746319540645 2308 70-71 -2.947599451338963 2308 72-73 -2.7152624925190736 2308 74-75 -2.5519811308530294 2308 76-77 -3.028956770388735 2308 78-79 -2.738583452971273 2308 80-81 -2.922299401330932 2308 82-83 -2.91858968096896 2308 84-85 -3.225122960255664 2308 86-87 -3.5739224965456273 2308 88-89 -3.753939646576441 2308 90-91 -4.147558228920595 2308 92-93 -3.777945940227017 2308 94-95 -4.270784254452128 2308 96-97 -4.647222340229604 2308 98-99 -4.665380458688141 2308 100-101 -4.439230320321897 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 427.0 11 3562.0 12 10788.0 13 21201.0 14 33014.0 15 46587.0 16 62712.0 17 91813.0 18 150002.0 19 288759.0 20 321743.0 21 294075.0 22 268005.0 23 248944.0 24 246448.0 25 256929.0 26 280723.0 27 309843.0 28 345479.0 29 389618.0 30 444266.0 31 525840.0 32 657383.0 33 860735.0 34 1210217.0 35 1824299.0 36 3002045.0 37 4953461.0 38 4475705.0 39 642550.0 40 184.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 24.321493371598013 31.511093502875376 22.91612682564317 21.251286299883443 2 21.90774046925549 26.097442723110664 33.467064211209966 18.527752596423877 3 21.11386541681326 29.337297222238938 23.530986478054558 26.017850882893246 4 26.73887041291562 24.015148092557727 28.27678972961229 20.969191764914367 5 21.705826079591482 29.52202052888358 23.36767568024909 25.404477711275852 6 21.96592527797529 24.00823321779949 27.388001189364324 26.637840314860895 7 26.932521586081293 24.207667564333406 23.214999282806698 25.644811566778603 8 31.2531643852854 23.993102369845584 23.48404332476264 21.269689920106373 9 21.201213004254928 28.312591013267042 23.74069254197108 26.74550344050695 10-11 28.453564181255807 23.866915419422458 23.631094896844072 24.048425502477663 12-13 26.09820617246105 23.899191363429825 23.708982448658706 26.29362001545042 14-15 25.943634624278282 23.916072575830505 23.823059745121085 26.317233054770128 16-17 25.965730238405076 26.47738705631122 23.79728739972558 23.759595305558125 18-19 23.296101104410372 23.974257474742064 26.535441543421612 26.194199877425955 20-21 23.07612784597077 28.656432882607806 24.26906236474035 23.99837690668107 22-23 20.709257470059985 26.244467349920903 29.417012561615973 23.629262618403136 24-25 20.730369090037534 23.729382264388978 32.084839162329004 23.455409483244487 26-27 20.740819364380812 23.723589031083076 34.487133588097876 21.048458016438232 28-29 20.740189974896158 23.657280029395558 34.573173980755335 21.029356014952953 30-31 20.73967849080631 23.623298599062842 34.575379847350824 21.06164306278003 32-33 20.754681899493686 23.63131426158025 34.556790600680685 21.057213238245385 34-35 20.815219822276816 23.658990160680915 34.535205210511634 20.990584806530634 36-37 20.803397842754006 23.620817034500135 34.51117332422815 21.064611798517703 38-39 20.979090525017686 23.73324153952674 33.95405975960936 21.333608175846216 40-41 21.690378309426332 24.30663758110144 31.855960300255838 22.147023809216392 42-43 21.70519117436183 24.525149928722133 31.98214215864217 21.78751673827387 44-45 21.802798886768635 24.928074241771686 31.511215621885764 21.757911249573915 46-47 21.65165734496738 25.301063488160956 31.389655533164696 21.65762363370697 48-49 21.659445745165126 25.414422228341294 31.106256371294005 21.819875655199574 50-51 21.690011020624826 25.554323057895555 30.917139182318138 21.83852673916148 52-53 21.717268237534654 25.625890784371226 30.80026721762844 21.85657376046568 54-55 21.898380921235635 25.54368381622711 30.52010804696418 22.037827215573078 56-57 22.029362441651138 25.54653767186929 30.30310780470678 22.12099208177279 58-59 22.17998538368413 25.648708484168225 29.972986357281844 22.1983197748658 60-61 22.289114301048173 25.784365760587747 29.534215792319387 22.392304146044697 62-63 22.460153027660787 25.955819977395983 29.13184979495589 22.452177199987343 64-65 22.577382103435887 26.10452483855516 28.799927678892125 22.518165379116823 66-67 22.719459189043594 26.148418391885624 28.437994432524587 22.694127986546192 68-69 22.80550856078329 26.239884272348018 28.194905971282765 22.75970119558593 70-71 22.88060203936654 26.334912984097826 28.001029741302872 22.783455235232765 72-73 22.96181526152285 26.366098644272622 27.792513426957914 22.879572667246613 74-75 23.065543048621592 26.38651036781691 27.665843573124782 22.882103010436712 76-77 23.113408873993464 26.46900188193328 27.509489327511382 22.90809991656187 78-79 23.16404065499875 26.398527524072097 27.449281014174687 22.98815080675447 80-81 23.19474388250017 26.403059878304582 27.37170097607387 23.030495263121374 82-83 23.24430947236979 26.487716270026834 27.281953253350817 22.986021004252564 84-85 23.237226897000145 26.467621805302606 27.215410879137007 23.07974041856024 86-87 23.277387473900426 26.531696528554676 27.121734586008262 23.06918141153664 88-89 23.306121032967887 26.56335176440592 27.09846849833707 23.032058704289124 90-91 23.355871879408642 26.52277996099578 26.964265232649765 23.15708292694581 92-93 23.36999311027894 26.52924783480585 26.980123414096035 23.120635640819167 94-95 23.413490487540066 26.583709431839164 26.930111065523416 23.072689015097353 96-97 23.45998166227422 26.553887385220527 26.83711998860387 23.149010963901382 98-99 23.519610620236058 26.601371096937054 26.82369578673033 23.055322496096558 100-101 23.75720267530153 26.474460448568248 26.640015265470403 23.128321610659818 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9130.0 1 5476.5 2 1477.5 3 1011.5 4 882.0 5 837.5 6 777.0 7 769.0 8 791.0 9 791.0 10 830.5 11 956.0 12 1323.5 13 2165.0 14 3400.0 15 5519.5 16 67687.5 17 88857.5 18 46192.5 19 39713.5 20 36698.5 21 39187.0 22 45147.5 23 54257.5 24 66794.5 25 84080.5 26 106593.5 27 132401.0 28 161685.5 29 193353.5 30 229793.5 31 271511.0 32 310865.5 33 346674.5 34 379110.5 35 416747.5 36 465891.0 37 522103.0 38 580581.5 39 637371.0 40 690310.0 41 732388.5 42 775102.5 43 817813.5 44 848768.0 45 876084.5 46 899860.0 47 912240.0 48 911459.0 49 900933.0 50 883611.5 51 861677.5 52 839976.0 53 805729.5 54 751840.5 55 684740.5 56 608908.0 57 534463.0 58 467506.0 59 402915.5 60 331877.0 61 273309.0 62 234977.0 63 198303.5 64 158533.0 65 122377.5 66 94346.0 67 72529.0 68 53481.5 69 39523.5 70 30071.0 71 24835.0 72 21644.5 73 16814.0 74 12124.5 75 8185.5 76 5238.0 77 3331.0 78 2021.5 79 1209.5 80 662.0 81 341.5 82 181.0 83 89.5 84 47.5 85 27.5 86 16.5 87 7.0 88 3.5 89 4.0 90 3.0 91 2.0 92 2.5 93 2.0 94 1.0 95 1.0 96 1.0 97 1.0 98 2.5 99 5.5 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 4.490878531705468E-6 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.9968818932178664E-4 18-19 4.490878531705468E-6 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.133167302560097E-4 30-31 7.028224902119057E-4 32-33 0.005546234986656252 34-35 0.0016616250567310232 36-37 7.679402289216348E-4 38-39 2.716981511681808E-4 40-41 0.0018569782728602108 42-43 0.0012439733532824146 44-45 0.002910089288545143 46-47 0.0026249185017818456 48-49 6.736317797558202E-6 50-51 8.083581357069842E-5 52-53 5.142055918802761E-4 54-55 1.7963514126821873E-5 56-57 0.0 58-59 0.0020702950031162206 60-61 6.624045834265565E-4 62-63 3.4355220767546825E-4 64-65 0.0 66-67 0.0015875255609578828 68-69 0.005784251548836643 70-71 0.0070574156125751426 72-73 0.0013809451484994312 74-75 0.0042842981192470165 76-77 0.00520717365751249 78-79 0.008992984259740199 80-81 0.0049983478057881855 82-83 9.004211456069462E-4 84-85 2.2229848731942066E-4 86-87 0.0010553564549507849 88-89 7.207860043387276E-4 90-91 1.0778108476093123E-4 92-93 5.770778913241527E-4 94-95 6.377047515021765E-4 96-97 6.624045834265565E-4 98-99 6.758772190216729E-4 100-101 4.98487517019307E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.2267358E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.65190559115879 #Duplication Level Percentage of deduplicated Percentage of total 1 74.2329135976737 37.60038531306016 2 13.127424403602742 13.298581230927201 3 4.960114513122286 7.537177561200196 4 2.488223730451177 5.041330939379759 5 1.4013060282941576 3.548941032473868 6 0.8855032891734782 2.691145740232536 7 0.5672244052931685 2.0111697918137534 8 0.4121415160548608 1.670060252912629 9 0.3060440432486607 1.3951542586830912 >10 1.4846672690748373 13.899256766867008 >50 0.10505685776723299 3.624431423292192 >100 0.028286455520704523 2.1782976764213684 >500 4.7188742614775287E-4 0.16122089440658538 >1k 4.40752087492257E-4 0.4430929377813581 >5k 1.0357211966000626E-4 0.36408546251730506 >10k+ 7.767908974480638E-5 4.535668718031024 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 798832 3.587457479239342 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 81266 0.36495573475757653 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58949 0.26473279856550563 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34788 0.15622868236096982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014640264013359825 0.0 0.0 0.0 0.0 2 0.001468517279867688 0.0 0.0 0.0 0.0 3 0.001477499036931099 0.0 0.0 8.981757063410936E-6 0.0 4 0.0014864807939945098 4.490878531705468E-6 0.0 8.981757063410936E-6 0.0 5 0.0014999534295896263 4.490878531705468E-6 0.0 1.3472635595116404E-5 0.0 6 0.0015224078222481536 4.490878531705468E-6 0.0 1.3472635595116404E-5 0.0 7 0.0015268987007798591 4.490878531705468E-6 0.0 1.3472635595116404E-5 4.490878531705468E-6 8 0.0015403713363749754 4.490878531705468E-6 0.0 1.3472635595116404E-5 4.490878531705468E-6 9 0.0016212071499456739 4.490878531705468E-6 0.0 1.7963514126821873E-5 4.490878531705468E-6 10-11 0.0016728522530602868 4.490878531705468E-6 0.0 1.7963514126821873E-5 6.736317797558202E-6 12-13 0.0017806333378212178 4.490878531705468E-6 0.0 2.0208953392674605E-5 8.981757063410936E-6 14-15 0.0019512867220260258 6.736317797558202E-6 0.0 2.4699831924380074E-5 8.981757063410936E-6 16-17 0.0023217842008917268 8.981757063410936E-6 0.0 2.919071045608554E-5 1.796351412682187E-5 18-19 0.0031031970654084784 1.3472635595116404E-5 0.0 3.143614972193827E-5 2.2454392658527338E-5 20-21 0.003981163818356897 1.3472635595116404E-5 0.0 3.5927028253643745E-5 2.6945271190232807E-5 22-23 0.005186964704119815 1.3472635595116404E-5 0.0 3.5927028253643745E-5 3.143614972193827E-5 24-25 0.007097833519360492 1.3472635595116404E-5 0.0 3.5927028253643745E-5 3.5927028253643745E-5 26-27 0.009170373961742565 1.3472635595116404E-5 0.0 3.5927028253643745E-5 3.5927028253643745E-5 28-29 0.010712990737383393 1.5718074860969136E-5 0.0 3.5927028253643745E-5 3.817246751949648E-5 30-31 0.011642602593446426 1.7963514126821873E-5 0.0 3.5927028253643745E-5 4.715422458290741E-5 32-33 0.012468924243280232 1.7963514126821873E-5 0.0 3.5927028253643745E-5 5.613598164631835E-5 34-35 0.013566944044282218 1.7963514126821873E-5 0.0 4.715422458290741E-5 5.838142091217108E-5 36-37 0.014700890873537847 1.7963514126821873E-5 0.0 5.3890542380465614E-5 6.062686017802381E-5 38-39 0.015628257290335026 2.2454392658527338E-5 0.0 5.3890542380465614E-5 6.287229944387655E-5 40-41 0.01643661542604201 2.2454392658527338E-5 0.0 5.3890542380465614E-5 6.736317797558201E-5 42-43 0.01727191883293923 2.2454392658527338E-5 0.0 5.838142091217108E-5 7.185405650728749E-5 44-45 0.018322784409358306 2.2454392658527338E-5 0.0 6.062686017802381E-5 7.409949577314023E-5 46-47 0.01941631333182859 2.2454392658527338E-5 0.0 6.287229944387655E-5 8.083581357069842E-5 48-49 0.020514333132830576 2.2454392658527338E-5 0.0 6.287229944387655E-5 8.083581357069842E-5 50-51 0.021540498877325277 2.6945271190232807E-5 0.0 6.287229944387655E-5 8.532669210240389E-5 52-53 0.02290123507243203 2.6945271190232807E-5 0.0 6.736317797558201E-5 8.532669210240389E-5 54-55 0.024836803719597088 3.143614972193827E-5 0.0 7.634493503899296E-5 8.981757063410935E-5 56-57 0.026749917974103615 3.368158898779101E-5 0.0 8.532669210240389E-5 9.206300989996209E-5 58-59 0.02843624286275902 4.2663346051201943E-5 0.0 8.981757063410935E-5 9.655388843166754E-5 60-61 0.030648000539623965 4.4908785317054676E-5 0.0 9.206300989996209E-5 1.0329020622922576E-4 62-63 0.033250464648747285 4.4908785317054676E-5 0.0 9.879932769752028E-5 1.0329020622922576E-4 64-65 0.03636039803195332 4.4908785317054676E-5 0.0 1.0104476696337302E-4 1.0329020622922576E-4 66-67 0.04011477248445909 4.939966384876014E-5 0.0 1.0329020622922576E-4 1.0329020622922576E-4 68-69 0.04441703411783293 4.939966384876014E-5 0.0 1.0778108476093123E-4 1.0778108476093123E-4 70-71 0.05386584254854123 4.939966384876014E-5 0.0 1.1451740255848943E-4 1.122719632926367E-4 72-73 0.06432509864888326 5.1645103114612875E-5 0.0 1.1676284182434216E-4 1.1451740255848943E-4 74-75 0.07273651413876761 5.3890542380465614E-5 0.0 1.1900828109019489E-4 1.1676284182434216E-4 76-77 0.08344725943688515 5.3890542380465614E-5 0.0 1.2349915962190036E-4 1.2349915962190036E-4 78-79 0.09681660482577233 5.3890542380465614E-5 0.0 1.2799003815360584E-4 1.257445988877531E-4 80-81 0.11382580726460678 5.838142091217108E-5 0.0 1.3023547741945857E-4 1.257445988877531E-4 82-83 0.1342615500231325 6.062686017802381E-5 0.0 1.3472635595116402E-4 1.257445988877531E-4 84-85 0.16029292743216328 6.287229944387655E-5 0.0 1.3472635595116402E-4 1.257445988877531E-4 86-87 0.1927754518519889 6.287229944387655E-5 0.0 1.3472635595116402E-4 1.257445988877531E-4 88-89 0.23369184615435742 6.736317797558201E-5 0.0 1.4370811301457498E-4 1.2799003815360584E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 115960 0.0 93.74867 1 TCGACGG 118810 0.0 91.5638 2 TATCTCG 110675 0.0 90.7924 2 ATATCTC 113705 0.0 88.56095 1 ACGGCGC 124825 0.0 87.14016 5 CGACGGC 124885 0.0 87.08688 3 CGGCGCG 125785 0.0 86.44111 6 GACGGCG 129770 0.0 83.85987 4 ATCTCGA 120190 0.0 83.46244 3 TCTCGAG 121295 0.0 81.13968 4 CTCGAGG 125720 0.0 78.348 5 TCGAGGG 127005 0.0 77.44685 6 AGGGCGC 132065 0.0 74.64855 9 GAGGGCG 135110 0.0 73.043526 8 CGAGGGC 136475 0.0 72.114586 7 CGCGCCG 252700 0.0 44.89371 9 GCGCGCC 254050 0.0 44.67758 8 GGCGCGC 254235 0.0 44.646935 7 ATCCATA 135330 0.0 40.037388 16-17 TCCATAT 136685 0.0 39.706158 18-19 >>END_MODULE