FastQCFastQC Report
Tue 31 May 2016
SRR1051279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22267358
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT841260.37779964735825416No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA509310.22872493449829118No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448470.20140242951139511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG142550.085.536851
TCGACGG144900.084.347522
ATCTCGA184450.063.398991
CGACGGC206700.059.3339233
ACGGCGC208200.058.8608135
CGGCGCG212850.057.4633376
TCTCGAG220250.053.0111122
GACGGCG265000.046.4776234
CTCGAGG278600.041.7877963
TCGAGGG287500.040.5437554
GGGCGCG315650.036.8527838
CGCGCCG519800.035.5559439
GGCGCGC524500.035.2282757
GCGCGCC529500.034.913568
AGGGCGC343250.034.1247757
GAGGGCG375150.031.1724116
CGAGGGC380850.030.7557565
ATCCATA285050.022.32909216-17
TCCATAT298600.020.97382518-19
GCCGGAT522300.020.48481412-13
Produced by FastQC (version 0.11.5)