##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051279_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22267358 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07627159899257 33.0 31.0 34.0 31.0 34.0 2 32.37548751854621 33.0 31.0 34.0 31.0 34.0 3 32.40508519241484 34.0 31.0 34.0 31.0 34.0 4 36.101914245955896 37.0 35.0 37.0 35.0 37.0 5 36.053038398179076 37.0 35.0 37.0 35.0 37.0 6 36.03019424217278 37.0 35.0 37.0 35.0 37.0 7 36.03062424379219 37.0 35.0 37.0 35.0 37.0 8 36.02277342466942 37.0 35.0 37.0 35.0 37.0 9 37.73972700308676 39.0 37.0 39.0 35.0 39.0 10-11 37.74215766863765 39.0 37.0 39.0 35.0 39.0 12-13 37.7402345846328 39.0 37.0 39.0 35.0 39.0 14-15 39.08862667946507 40.0 38.0 41.0 36.0 41.0 16-17 39.06067738256151 40.0 38.0 41.0 36.0 41.0 18-19 39.05842978767396 40.0 38.5 41.0 36.0 41.0 20-21 39.07821484704203 40.0 39.0 41.0 36.0 41.0 22-23 39.04946361395905 40.0 39.0 41.0 36.0 41.0 24-25 39.00109532527388 40.0 38.0 41.0 35.5 41.0 26-27 38.84331609973667 40.0 38.0 41.0 35.0 41.0 28-29 38.66923741020376 40.0 38.0 41.0 35.0 41.0 30-31 38.504336145311896 40.0 38.0 41.0 35.0 41.0 32-33 38.6312134784917 40.0 38.0 41.0 35.0 41.0 34-35 38.62643399365116 40.0 38.0 41.0 35.0 41.0 36-37 38.57113288877827 40.0 38.0 41.0 35.0 41.0 38-39 38.504714883552865 40.0 38.0 41.0 34.5 41.0 40-41 38.38721755854466 40.0 38.0 41.0 34.0 41.0 42-43 38.295546085889484 40.0 38.0 41.0 34.0 41.0 44-45 38.19828396345898 40.0 38.0 41.0 33.5 41.0 46-47 38.06491171965709 40.0 37.5 41.0 33.0 41.0 48-49 38.001239954016995 40.0 37.0 41.0 33.0 41.0 50-51 38.04134008623744 40.0 37.0 41.0 33.0 41.0 52-53 37.82927262857138 40.0 37.0 41.0 33.0 41.0 54-55 37.62845042056628 40.0 36.5 41.0 33.0 41.0 56-57 37.478084086131815 39.0 36.0 41.0 33.0 41.0 58-59 37.23060398543913 39.0 36.0 41.0 32.0 41.0 60-61 37.00121781398583 39.0 35.0 41.0 32.0 41.0 62-63 36.66260617896384 38.5 35.0 40.0 31.0 41.0 64-65 36.324252432641536 38.0 35.0 40.0 31.0 41.0 66-67 35.92681141157384 37.0 35.0 40.0 31.0 41.0 68-69 35.54363764214865 37.0 34.5 39.0 30.5 41.0 70-71 35.15668459634951 36.0 34.0 39.0 30.0 40.5 72-73 34.79213717226803 36.0 34.0 39.0 30.0 40.0 74-75 34.447632067531316 35.0 34.0 37.5 30.0 39.5 76-77 33.896665356527706 35.0 33.5 37.0 29.0 39.0 78-79 33.71664790677008 35.0 34.0 36.5 29.0 39.0 80-81 33.39566083681773 35.0 33.5 36.0 29.0 37.5 82-83 33.05972071316229 35.0 33.0 36.0 29.0 37.0 84-85 32.85258852891304 35.0 33.0 35.0 29.0 37.0 86-87 32.61625314956538 35.0 33.0 35.0 28.5 36.0 88-89 32.366794727061915 35.0 33.0 35.0 27.0 36.0 90-91 32.188449478379965 35.0 33.0 35.0 27.0 36.0 92-93 31.974224490395315 35.0 33.0 35.0 27.0 35.0 94-95 31.80657628983196 35.0 33.0 35.0 26.0 35.0 96-97 31.661957561377513 35.0 33.0 35.0 26.0 35.0 98-99 31.366226451292516 34.5 32.0 35.0 25.0 35.0 100-101 29.861720730407264 33.5 30.0 34.5 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29097896657570743 1101 2 0.23358594390187193 1101 3 0.1090966665898776 1101 4 0.16228322913483595 1101 5 0.26613968114747877 1101 6 0.31361191038016756 1101 7 0.3390995989224663 1101 8 0.34826603032055914 1101 9 0.4480168047679598 1101 10-11 0.462944634994102 1101 12-13 0.44945797406481347 1101 14-15 0.5706127659450857 1101 16-17 0.5466155134209458 1101 18-19 0.5467123299718679 1101 20-21 0.5173042730704864 1101 22-23 0.5247572576328636 1101 24-25 0.5321486359333463 1101 26-27 0.5895801446786066 1101 28-29 0.5972850135264594 1101 30-31 0.6052163886956663 1101 32-33 0.5731155277095255 1101 34-35 0.5851743207886031 1101 36-37 0.52424998730136 1101 38-39 0.499758156302029 1101 40-41 0.4631549775283972 1101 42-43 0.4466711444276257 1101 44-45 0.4289141556644367 1101 46-47 0.43792263894407313 1101 48-49 0.4794544380790171 1101 50-51 0.5333549276347256 1101 52-53 0.57474000105492 1101 54-55 0.6002557762014646 1101 56-57 0.5151788493708906 1101 58-59 0.5360628936824554 1101 60-61 0.5344864663989028 1101 62-63 0.5370603090903643 1101 64-65 0.5863518197921493 1101 66-67 0.5483663846688955 1101 68-69 0.5343619965090269 1101 70-71 0.5160134168240518 1101 72-73 0.558428161001892 1101 74-75 0.4898771473784862 1101 76-77 0.43758643702543054 1101 78-79 0.5117361208638229 1101 80-81 0.5141429706995169 1101 82-83 0.5812628395347588 1101 84-85 0.5002352884875663 1101 86-87 0.5138170828968498 1101 88-89 0.5214002275800169 1101 90-91 0.537841849025817 1101 92-93 0.5406231892266717 1101 94-95 0.5598020666031758 1101 96-97 0.49503279990535987 1101 98-99 0.6415539017003482 1101 100-101 1.0985168738856181 1102 1 0.3155033026691001 1102 2 0.27174669129995976 1102 3 0.12303660605699918 1102 4 0.16429927624889729 1102 5 0.24869186261125975 1102 6 0.2725078429267853 1102 7 0.286194220370092 1102 8 0.27826043701913505 1102 9 0.3814956847626405 1102 10-11 0.37291507571484317 1102 12-13 0.3818625479840847 1102 14-15 0.5040480818856992 1102 16-17 0.45733287428884495 1102 18-19 0.4459902898665149 1102 20-21 0.4086749545850452 1102 22-23 0.4046282157703658 1102 24-25 0.44219635012033365 1102 26-27 0.4692643282623479 1102 28-29 0.512432311845302 1102 30-31 0.5008779153553533 1102 32-33 0.433752301784061 1102 34-35 0.4788188267856057 1102 36-37 0.4716899728750832 1102 38-39 0.41471621707231066 1102 40-41 0.3836414328203759 1102 42-43 0.38190850651807295 1102 44-45 0.3972944883948202 1102 46-47 0.40643214476798306 1102 48-49 0.42747543447328695 1102 50-51 0.4481889396720149 1102 52-53 0.5000019339947954 1102 54-55 0.5596103872767344 1102 56-57 0.4826011104086092 1102 58-59 0.45590590722683544 1102 60-61 0.49015854844246576 1102 62-63 0.5042219197095505 1102 64-65 0.5079789485513899 1102 66-67 0.5102811926317656 1102 68-69 0.4898260942852559 1102 70-71 0.4740511304826356 1102 72-73 0.5116343292658883 1102 74-75 0.48626335687037425 1102 76-77 0.40969268976628825 1102 78-79 0.45609042029037994 1102 80-81 0.4709108264808535 1102 82-83 0.5265238518870561 1102 84-85 0.47392334704910866 1102 86-87 0.4702560280230159 1102 88-89 0.5100659042887514 1102 90-91 0.5227263704030349 1102 92-93 0.5517441226426953 1102 94-95 0.5744019122724566 1102 96-97 0.5299015913330294 1102 98-99 0.6072440403448667 1102 100-101 1.1458600010572688 1103 1 0.24673704990457423 1103 2 0.23739489826174776 1103 3 0.18939565013024406 1103 4 0.18820379183374314 1103 5 0.2235145194913457 1103 6 0.25734718169707094 1103 7 0.24512280736738745 1103 8 0.2224222002373324 1103 9 0.32134656321655797 1103 10-11 0.31732408202354634 1103 12-13 0.30528450451523526 1103 14-15 0.41673932346112963 1103 16-17 0.41295190358223977 1103 18-19 0.344034275636929 1103 20-21 0.3373748232068934 1103 22-23 0.32110031958054464 1103 24-25 0.3498927087183361 1103 26-27 0.35531298975378434 1103 28-29 0.3853791843901462 1103 30-31 0.4050152539354599 1103 32-33 0.33287598107924765 1103 34-35 0.3339718958763811 1103 36-37 0.3629684682320402 1103 38-39 0.2987452793893439 1103 40-41 0.2661688852459463 1103 42-43 0.23944647388002238 1103 44-45 0.27919829942185004 1103 46-47 0.28529570099932755 1103 48-49 0.32950156849199175 1103 50-51 0.3389650350716593 1103 52-53 0.4604585802652892 1103 54-55 0.44715742588867613 1103 56-57 0.3920388713626721 1103 58-59 0.3847462892332558 1103 60-61 0.36010629375812186 1103 62-63 0.38953504490259405 1103 64-65 0.3990915850301562 1103 66-67 0.3593759069302749 1103 68-69 0.3482247955015012 1103 70-71 0.3536661619437922 1103 72-73 0.4242647669643844 1103 74-75 0.35750822949112404 1103 76-77 0.293804741079164 1103 78-79 0.3965503529174299 1103 80-81 0.37330899430380526 1103 82-83 0.43096116277775565 1103 84-85 0.3730696736854 1103 86-87 0.3512828686353089 1103 88-89 0.4096087318769719 1103 90-91 0.4075923321204087 1103 92-93 0.44464613698664124 1103 94-95 0.47108593450632696 1103 96-97 0.46420530800237003 1103 98-99 0.596475075728236 1103 100-101 1.0358878010931107 1104 1 0.014294532044097252 1104 2 -0.04141659403743603 1104 3 -0.15978983689641524 1104 4 -0.07037729460137854 1104 5 0.03342013460526516 1104 6 0.13612663576648032 1104 7 0.23168725564510595 1104 8 0.22273320116152462 1104 9 0.3451427458920193 1104 10-11 0.32897802181710745 1104 12-13 0.3333768461900064 1104 14-15 0.4363057666520973 1104 16-17 0.4561683011396056 1104 18-19 0.4065071619037042 1104 20-21 0.3167136051423398 1104 22-23 0.26488149108199366 1104 24-25 0.27436037808105596 1104 26-27 0.3182723476817131 1104 28-29 0.3737203580450128 1104 30-31 0.3986325005013356 1104 32-33 0.373869587708235 1104 34-35 0.2787081625987611 1104 36-37 0.3291685629360188 1104 38-39 0.30722991630400287 1104 40-41 0.2218214720349252 1104 42-43 0.30320529782078154 1104 44-45 0.36269574419958417 1104 46-47 0.34251805152178605 1104 48-49 0.28654966709335383 1104 50-51 0.32168897110574335 1104 52-53 0.37345707286007723 1104 54-55 0.43784603368951025 1104 56-57 0.28935311254618057 1104 58-59 0.28447400760226316 1104 60-61 0.2671850084340406 1104 62-63 0.23856306763295265 1104 64-65 0.32314336361029916 1104 66-67 0.3452568615828753 1104 68-69 0.16877010311065277 1104 70-71 0.20724929460141084 1104 72-73 0.3242241186167121 1104 74-75 0.3067472504490283 1104 76-77 0.22104955115914038 1104 78-79 0.3078004964257559 1104 80-81 0.2826626405146868 1104 82-83 0.34644561533850293 1104 84-85 0.29736587228479294 1104 86-87 0.22522240098728474 1104 88-89 0.2710958411023796 1104 90-91 0.24308909542296675 1104 92-93 0.3488248155615068 1104 94-95 0.45437896144177614 1104 96-97 0.32642620660854504 1104 98-99 0.4738171605844741 1104 100-101 0.781351433929931 1105 1 0.07343175729567974 1105 2 0.12650950974079223 1105 3 0.19299477976107937 1105 4 0.1352183358346437 1105 5 0.18746652146653986 1105 6 0.1635183642204936 1105 7 0.16672748562067596 1105 8 0.15743632798366036 1105 9 0.20530268357637738 1105 10-11 0.23784570768501823 1105 12-13 0.2166496621495213 1105 14-15 0.28338474109889944 1105 16-17 0.23858944683998118 1105 18-19 0.21095127864336405 1105 20-21 0.06433127531149552 1105 22-23 0.09759165371087164 1105 24-25 0.1641931676559949 1105 26-27 0.19048438435103776 1105 28-29 0.19983038048701474 1105 30-31 0.16664931652819348 1105 32-33 0.20270340425059175 1105 34-35 0.2179368364348946 1105 36-37 0.1638624512829523 1105 38-39 0.13650139076937506 1105 40-41 0.0920197536608427 1105 42-43 0.12194926994757083 1105 44-45 0.12618327757564174 1105 46-47 0.1414050895858452 1105 48-49 0.19800346088956644 1105 50-51 0.21649902697758705 1105 52-53 0.2250864310478633 1105 54-55 0.28057757991717835 1105 56-57 0.19210515268081707 1105 58-59 0.1788803607141105 1105 60-61 0.1464286973121034 1105 62-63 0.164146501413029 1105 64-65 0.22553344001758546 1105 66-67 0.22772875063142806 1105 68-69 0.2588225659435395 1105 70-71 0.2509911586591045 1105 72-73 0.2349148501258611 1105 74-75 0.2734111257026015 1105 76-77 0.1798945523854414 1105 78-79 0.2543061950825347 1105 80-81 0.2656169691005559 1105 82-83 0.36616847934401875 1105 84-85 0.29825711960763357 1105 86-87 0.22467227833060122 1105 88-89 0.2215240681657633 1105 90-91 0.3062084388379205 1105 92-93 0.3251857897065449 1105 94-95 0.4041155868644424 1105 96-97 0.27979931558448357 1105 98-99 0.36281797511304603 1105 100-101 0.7107870240972467 1106 1 0.02967114546228089 1106 2 0.07297779726064135 1106 3 0.17322769474417754 1106 4 0.10671359409764847 1106 5 0.0967692970358911 1106 6 0.06872429902387012 1106 7 0.11146524977817052 1106 8 0.07389618811301091 1106 9 0.022252589511005283 1106 10-11 0.09367487799337937 1106 12-13 0.08018397865119198 1106 14-15 0.11726924654368531 1106 16-17 0.07840669007862289 1106 18-19 0.0641689217603556 1106 20-21 0.05603702507193731 1106 22-23 0.058467000562266946 1106 24-25 0.05093772129030327 1106 26-27 0.030037222074561498 1106 28-29 0.028021184152265732 1106 30-31 0.040269095060594395 1106 32-33 -0.0025844504438268245 1106 34-35 0.012425235021204628 1106 36-37 -0.03170202001244604 1106 38-39 -0.0503285009483605 1106 40-41 -0.08033475949616076 1106 42-43 -0.09710676571468468 1106 44-45 -0.13253038307488652 1106 46-47 -0.12959438841181736 1106 48-49 -0.142456486113673 1106 50-51 -0.0803623171771477 1106 52-53 -0.1223356702092957 1106 54-55 -0.05995437324473585 1106 56-57 -0.10117752149250236 1106 58-59 -0.1528853403559296 1106 60-61 -0.1276951941822091 1106 62-63 -0.10616972274989678 1106 64-65 -0.0683805314661825 1106 66-67 -0.13522799392814022 1106 68-69 -0.07597963261236629 1106 70-71 -0.038001486104775495 1106 72-73 -0.033113821735483384 1106 74-75 -0.033477732762243306 1106 76-77 -0.19183034029860124 1106 78-79 -0.1543427256708867 1106 80-81 -0.13800740731858951 1106 82-83 -0.08572825207180301 1106 84-85 -0.09077381188555478 1106 86-87 -0.14829552398133217 1106 88-89 -0.19481290935760853 1106 90-91 -0.2253985096844886 1106 92-93 -0.25460964424405574 1106 94-95 -0.21507450717483678 1106 96-97 -0.3553849022041078 1106 98-99 -0.2708264390627093 1106 100-101 0.06866459581288353 1107 1 -0.0648219064482376 1107 2 -3.472690073635931E-5 1107 3 0.11194448549805713 1107 4 0.06628883269901564 1107 5 0.039370954583375806 1107 6 -0.004513687778199937 1107 7 0.008734225534958284 1107 8 -0.009844470344127387 1107 9 -0.033957677123837016 1107 10-11 0.02911476542151803 1107 12-13 0.007232149736438487 1107 14-15 -0.005115554852615389 1107 16-17 -0.057796371878339414 1107 18-19 -0.08336841659512118 1107 20-21 -0.09648508444528403 1107 22-23 -0.08241726552355289 1107 24-25 -0.0774336796898254 1107 26-27 -0.10128902084929337 1107 28-29 -0.060027200954849036 1107 30-31 -0.09988467425637282 1107 32-33 -0.08726779690029929 1107 34-35 -0.11251716619928942 1107 36-37 -0.1380681619133881 1107 38-39 -0.17324690276102928 1107 40-41 -0.2232240034162416 1107 42-43 -0.22827183320490008 1107 44-45 -0.21957037923311162 1107 46-47 -0.1850465883461112 1107 48-49 -0.21106610204345344 1107 50-51 -0.16200207810422995 1107 52-53 -0.22259967617687693 1107 54-55 -0.24675796763665403 1107 56-57 -0.27200042777508315 1107 58-59 -0.20416825789073556 1107 60-61 -0.24803984699385495 1107 62-63 -0.2880757845352946 1107 64-65 -0.26215633439060326 1107 66-67 -0.3063721648980646 1107 68-69 -0.21445216673718903 1107 70-71 -0.16940077353091 1107 72-73 -0.20761262550819026 1107 74-75 -0.23255796008406548 1107 76-77 -0.42927928949991667 1107 78-79 -0.27664578287384245 1107 80-81 -0.2921404115035031 1107 82-83 -0.23614833101463972 1107 84-85 -0.24863409987904106 1107 86-87 -0.24816478677549014 1107 88-89 -0.2538779858899147 1107 90-91 -0.1851931927574597 1107 92-93 -0.2612629317970203 1107 94-95 -0.20634277843483062 1107 96-97 -0.32172119254132525 1107 98-99 -0.29228762304558487 1107 100-101 0.05031573131388356 1108 1 -0.24668349112450016 1108 2 -0.1281034669129184 1108 3 0.02956307762220689 1108 4 0.03110552745678774 1108 5 0.01105731855462011 1108 6 -0.7761632147627893 1108 7 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-1.6180302683118413 2308 80-81 -1.2925500400798242 2308 82-83 -0.8976429899020317 2308 84-85 -1.0233736171340198 2308 86-87 -0.8967145990415091 2308 88-89 -1.1541282594136781 2308 90-91 -1.1534589509297675 2308 92-93 -1.2289242364862538 2308 94-95 -1.6105930557568158 2308 96-97 -1.467920583489164 2308 98-99 -1.209512538281814 2308 100-101 -1.5132095814874837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 8.0 11 63.0 12 335.0 13 1377.0 14 4251.0 15 9309.0 16 16271.0 17 25647.0 18 42719.0 19 75381.0 20 82718.0 21 80429.0 22 77097.0 23 79972.0 24 87637.0 25 103540.0 26 129664.0 27 165505.0 28 211313.0 29 270585.0 30 347429.0 31 446704.0 32 584602.0 33 786560.0 34 1130641.0 35 1754124.0 36 3031509.0 37 5678583.0 38 6034844.0 39 1008003.0 40 538.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.45146966836288 29.083281387078312 25.155649924085928 23.30959902047288 2 23.714332677790388 27.81538479502644 27.334892324937922 21.13539020224525 3 22.494783170016518 26.549068200918708 26.63415689174385 24.32199173732092 4 24.133864466543358 26.31494495215822 26.509952370640466 23.041238210657948 5 24.031979905294556 27.318341942497177 25.825906243569623 22.823771908638648 6 24.37876105463432 26.302065112529288 25.24140043915403 24.07777339368236 7 24.6732638869865 26.72728394630382 25.6295560524064 22.96989611430328 8 24.386804218084606 26.167814789702486 25.95937066265338 23.48601032955953 9 23.532989409879697 25.75313155696334 26.271652883112584 24.442226150044384 10-11 23.752119582395 26.147545209449635 26.08453818364981 24.015797024505556 12-13 23.792827150845646 26.15541547407645 26.18027248674944 23.871484888328467 14-15 23.560590349335563 26.239702527798762 26.286396437332172 23.913310685533506 16-17 23.691679093676044 26.51213493760688 26.245289180692204 23.550896788024875 18-19 23.158591603009214 26.353038829303415 26.815096339673527 23.67327322801385 20-21 22.91147427548432 26.28808051678156 26.83529406586987 23.96515114186425 22-23 22.949015774570114 26.243782490944817 27.41217435853863 23.39502737594644 24-25 22.969920814135204 25.899772213659116 27.955209145153187 23.175097827052497 26-27 23.011450213357147 25.9104784680787 27.982834784440975 23.095236534123174 28-29 23.02168717097017 25.98114693265362 27.95819782481604 23.038968071560173 30-31 23.050312030731263 25.915501515716414 27.915105599865058 23.11908085368727 32-33 23.042405839076192 25.955903704426902 27.919183317571843 23.082507138925056 34-35 23.10294512712285 26.008707454202696 27.869231275663687 23.01911614301077 36-37 23.084312472094805 25.962226861399547 27.850677211009945 23.102783455495707 38-39 23.125323174846336 26.026370079467892 27.704323970540194 23.143982775145574 40-41 23.208786152358087 26.15106830365776 27.4197055618363 23.22043998214786 42-43 23.233319821776792 26.160766355846977 27.396871689941843 23.20904213243439 44-45 23.203527333597457 26.252052443760952 27.36180915580555 23.18261106683604 46-47 23.240446846006606 26.372735822543476 27.25637904595597 23.130438285493952 48-49 23.2227258393205 26.353826978485728 27.207383561175064 23.216063621018712 50-51 23.177078304484976 26.395756065896997 27.2352292535109 23.191936376107126 52-53 23.23837206012496 26.44838691684932 27.17316306676347 23.140077956262257 54-55 23.225649401244638 26.45131496965199 27.103305205763522 23.21973042333985 56-57 23.21565270563306 26.522102442508 27.065718797892412 23.19652605396653 58-59 23.24992484514777 26.578065525330846 27.020976175081028 23.151033454440352 60-61 23.2391781728214 26.583025700669115 26.898465008736107 23.27933111777338 62-63 23.26269914913121 26.650530341318447 26.848248004994574 23.23852250455577 64-65 23.28425087520486 26.735360791343094 26.771635862682945 23.208752470769095 66-67 23.290609959205756 26.704681354653747 26.72699428463853 23.277714401501964 68-69 23.295553740292434 26.76760934779124 26.678537256993213 23.258299654923114 70-71 23.310982829664837 26.85356745061538 26.609483711538655 23.225966008181125 72-73 23.31078671784354 26.832582398227217 26.57755202819842 23.279078855730823 74-75 23.297386694910102 26.882915341820073 26.57754458342117 23.242153379848656 76-77 23.29241237054789 26.98017162477279 26.498268889708577 23.22914711497074 78-79 23.28464158163712 26.97879559847199 26.466514348042548 23.270048471848344 80-81 23.226607425683714 27.039156285292172 26.481428942088364 23.25280734693575 82-83 23.26496294497611 27.108295301839814 26.44503096492519 23.181710788258886 84-85 23.224659162528397 27.103832883990997 26.403965391852953 23.267542561627653 86-87 23.205896272022933 27.170324831531428 26.4075985125851 23.21618038386054 88-89 23.247736950521 27.23184292054092 26.361762013534207 23.158658115403874 90-91 23.223388243903926 27.238303259865855 26.324878775470356 23.21342972075987 92-93 23.207968812465314 27.294365142016396 26.315840882425295 23.181825163092988 94-95 23.20656496752161 27.375970790950362 26.28303854179655 23.134425699731477 96-97 23.223353562126437 27.377078168587847 26.22944959442305 23.17011867486267 98-99 23.20833672769817 27.45539894422754 26.20748821882206 23.12877610925223 100-101 23.193615512006136 27.588733017143873 25.91936699414845 23.298284476701543 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 41602.0 1 24376.0 2 5515.0 3 3469.0 4 2944.5 5 2752.0 6 2665.0 7 2679.5 8 2637.0 9 2605.5 10 2723.0 11 2848.0 12 3381.5 13 4459.5 14 5747.5 15 7043.0 16 15709.0 17 18636.5 18 14062.5 19 15394.5 20 18309.5 21 23672.5 22 30258.5 23 38321.5 24 49386.0 25 64610.5 26 83742.5 27 104472.5 28 127182.5 29 152773.0 30 183222.5 31 219123.5 32 255228.5 33 291184.0 34 325679.0 35 366115.0 36 418423.0 37 477890.0 38 540199.0 39 603264.5 40 663532.0 41 711792.0 42 762640.5 43 815020.0 44 854241.0 45 888403.5 46 921592.0 47 942323.5 48 946375.0 49 943748.5 50 932297.0 51 914266.0 52 898157.5 53 868208.0 54 814077.0 55 743620.5 56 664379.5 57 586811.0 58 516426.5 59 445344.5 60 366689.5 61 301955.5 62 259917.5 63 219900.5 64 175659.0 65 135200.0 66 104299.5 67 80537.0 68 60312.5 69 45543.5 70 35251.5 71 29283.0 72 25688.0 73 20198.0 74 14497.5 75 9779.5 76 6268.0 77 4097.0 78 2581.5 79 1470.0 80 723.5 81 363.5 82 195.0 83 98.0 84 47.0 85 15.5 86 5.5 87 2.5 88 2.0 89 2.5 90 3.5 91 2.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11865799256472187 2 0.003718447424252127 3 1.7963514126821873E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.736317797558202E-6 70-71 0.0 72-73 4.4908785317054676E-5 74-75 0.0 76-77 6.736317797558202E-6 78-79 0.0 80-81 5.186964704119815E-4 82-83 3.008888616242663E-4 84-85 0.0 86-87 0.0 88-89 1.5718074860969136E-5 90-91 0.0 92-93 0.0 94-95 5.6135981646318354E-5 96-97 2.469983192438007E-5 98-99 2.694527119023281E-4 100-101 5.6135981646318354E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.2267358E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.00647155023488 #Duplication Level Percentage of deduplicated Percentage of total 1 72.91676011833475 37.192266505111434 2 13.509941661260957 13.7818890998088 3 5.285488176521605 8.087823069145564 4 2.654590835110549 5.416052476343218 5 1.514976738116823 3.8636808946011687 6 0.9399712953467133 2.8766771480483735 7 0.6303810843766297 2.2507460392243943 8 0.43734069842056933 1.78457647133989 9 0.3211747369703405 1.474379107553866 >10 1.638460480556409 15.459365114040668 >50 0.1173981381899779 4.076633994509848 >100 0.03324490390133399 2.493853238291078 >500 1.3074152764638833E-4 0.04250623022837574 >1k 6.144687492428781E-5 0.0940882763310194 >5k 3.508644034036148E-5 0.11660370237203507 >10k+ 4.385805042544363E-5 0.9888586330504261 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 84126 0.37779964735825416 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50931 0.22872493449829118 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44847 0.20140242951139511 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014864807939945098 8.981757063410936E-6 0.0 0.0 0.0 2 0.0014954625510579208 8.981757063410936E-6 0.0 4.490878531705468E-6 0.0 3 0.0015044443081213316 8.981757063410936E-6 0.0 8.981757063410936E-6 0.0 4 0.001508935186653037 8.981757063410936E-6 0.0 1.3472635595116404E-5 0.0 5 0.0015313895793115644 1.3472635595116404E-5 0.0 2.2454392658527338E-5 0.0 6 0.0015628257290335027 1.3472635595116404E-5 0.0 2.6945271190232807E-5 0.0 7 0.0015762983646286192 1.3472635595116404E-5 0.0 3.143614972193827E-5 0.0 8 0.001594261878755441 1.3472635595116404E-5 0.0 4.041790678534921E-5 0.0 9 0.0016212071499456739 2.2454392658527338E-5 0.0 4.041790678534921E-5 0.0 10-11 0.0016750976923261395 2.2454392658527338E-5 0.0 4.4908785317054676E-5 0.0 12-13 0.001771651580757807 2.6945271190232807E-5 0.0 4.4908785317054676E-5 0.0 14-15 0.0019467958434943203 2.6945271190232807E-5 0.0 4.4908785317054676E-5 0.0 16-17 0.0023217842008917268 2.919071045608554E-5 0.0 4.4908785317054676E-5 0.0 18-19 0.00309646074761092 4.041790678534921E-5 0.0 4.715422458290741E-5 0.0 20-21 0.00396769118276178 4.041790678534921E-5 0.0 6.062686017802381E-5 6.736317797558202E-6 22-23 0.00518247382558811 4.041790678534921E-5 0.0 6.511773870972927E-5 8.981757063410936E-6 24-25 0.00707987000523367 4.2663346051201943E-5 0.0 7.185405650728749E-5 8.981757063410936E-6 26-27 0.009134446933488923 4.4908785317054676E-5 0.0 7.634493503899296E-5 8.981757063410936E-6 28-29 0.01068604546619316 4.4908785317054676E-5 0.0 8.083581357069842E-5 1.3472635595116404E-5 30-31 0.011595448368863517 4.715422458290741E-5 0.0 8.083581357069842E-5 1.7963514126821873E-5 32-33 0.01236787947631686 5.1645103114612875E-5 0.0 8.083581357069842E-5 1.7963514126821873E-5 34-35 0.013405272417140821 5.613598164631835E-5 0.0 8.532669210240389E-5 1.7963514126821873E-5 36-37 0.014460628872091607 5.838142091217108E-5 0.0 9.430844916581482E-5 1.7963514126821873E-5 38-39 0.015291441400457118 6.062686017802381E-5 0.0 9.430844916581482E-5 1.7963514126821873E-5 40-41 0.016052645311581196 6.960861724143475E-5 0.0 9.430844916581482E-5 2.0208953392674605E-5 42-43 0.01681384922270527 7.634493503899296E-5 0.0 9.87993276975203E-5 2.2454392658527338E-5 44-45 0.017795106181882916 7.859037430484568E-5 0.0 1.0329020622922576E-4 2.4699831924380074E-5 46-47 0.018771872262528855 8.308125283655116E-5 0.0 1.0329020622922576E-4 2.6945271190232807E-5 48-49 0.019773338175099173 8.532669210240389E-5 0.0 1.0329020622922576E-4 2.6945271190232807E-5 50-51 0.02072764986308659 8.532669210240389E-5 0.0 1.055356454950785E-4 2.6945271190232807E-5 52-53 0.022000813926825086 8.532669210240389E-5 0.0 1.0778108476093123E-4 2.919071045608554E-5 54-55 0.02385105588188774 8.757213136825661E-5 0.0 1.122719632926367E-4 3.143614972193827E-5 56-57 0.02563393465897481 9.430844916581482E-5 0.0 1.1676284182434216E-4 3.143614972193827E-5 58-59 0.027239423734059513 9.655388843166754E-5 0.0 1.1676284182434216E-4 3.143614972193827E-5 60-61 0.029424236139734224 9.879932769752028E-5 0.0 1.2125372035604763E-4 3.143614972193827E-5 62-63 0.031984036902806344 1.0329020622922576E-4 0.0 1.2125372035604763E-4 3.143614972193827E-5 64-65 0.035001907276112416 1.0329020622922576E-4 0.0 1.2799003815360584E-4 3.143614972193827E-5 66-67 0.038639518886793844 1.1002652402678395E-4 0.0 1.324809166853113E-4 3.143614972193827E-5 68-69 0.04291932612750916 1.1451740255848943E-4 0.0 1.392172344828695E-4 3.143614972193827E-5 70-71 0.05234568016555893 1.2349915962190036E-4 0.0 1.392172344828695E-4 3.368158898779101E-5 72-73 0.06262530112463274 1.257445988877531E-4 2.245439265852734E-6 1.392172344828695E-4 3.5927028253643745E-5 74-75 0.07097608975433907 1.3023547741945857E-4 4.490878531705468E-6 1.4370811301457498E-4 3.5927028253643745E-5 76-77 0.08153639062164447 1.3472635595116402E-4 4.490878531705468E-6 1.4370811301457498E-4 4.041790678534921E-5 78-79 0.09464301961642688 1.3472635595116402E-4 4.490878531705468E-6 1.459535522804277E-4 4.2663346051201943E-5 80-81 0.11149504130665164 1.3472635595116402E-4 4.490878531705468E-6 1.526898700779859E-4 4.4908785317054676E-5 82-83 0.131692767502997 1.3472635595116402E-4 4.490878531705468E-6 1.5718074860969136E-4 4.4908785317054676E-5 84-85 0.15741876517187176 1.3472635595116402E-4 4.490878531705468E-6 1.5718074860969136E-4 4.715422458290741E-5 86-87 0.18963857319759264 1.3697179521701675E-4 4.490878531705468E-6 1.5718074860969136E-4 4.939966384876014E-5 88-89 0.23007668893633454 1.392172344828695E-4 4.490878531705468E-6 1.5942618787554412E-4 5.1645103114612875E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 14255 0.0 85.53685 1 TCGACGG 14490 0.0 84.34752 2 ATCTCGA 18445 0.0 63.39899 1 CGACGGC 20670 0.0 59.333923 3 ACGGCGC 20820 0.0 58.860813 5 CGGCGCG 21285 0.0 57.463337 6 TCTCGAG 22025 0.0 53.011112 2 GACGGCG 26500 0.0 46.477623 4 CTCGAGG 27860 0.0 41.787796 3 TCGAGGG 28750 0.0 40.543755 4 GGGCGCG 31565 0.0 36.852783 8 CGCGCCG 51980 0.0 35.555943 9 GGCGCGC 52450 0.0 35.228275 7 GCGCGCC 52950 0.0 34.91356 8 AGGGCGC 34325 0.0 34.124775 7 GAGGGCG 37515 0.0 31.172411 6 CGAGGGC 38085 0.0 30.755756 5 ATCCATA 28505 0.0 22.329092 16-17 TCCATAT 29860 0.0 20.973825 18-19 GCCGGAT 52230 0.0 20.484814 12-13 >>END_MODULE