FastQCFastQC Report
Tue 31 May 2016
SRR1051278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29830305
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT12217714.0957375393915685No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT1285630.4309811783687763No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1012150.33930259848164473No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT624330.20929387078006742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1574550.093.8286061
TCGACGG1614450.091.52442
TATCTCG1588600.091.207912
ATATCTC1631600.089.0021061
CGACGGC1700550.086.83463
ACGGCGC1700600.086.770615
CGGCGCG1711900.086.239466
GACGGCG1764550.083.728194
ATCTCGA1741000.083.169373
TCTCGAG1763200.080.778074
CTCGAGG1835200.077.593395
TCGAGGG1849950.076.959326
AGGGCGC1938900.073.634469
GAGGGCG1966350.072.637938
CGAGGGC1978650.072.0351647
CGCGCCG3593900.043.2224129
GCGCGCC3604200.043.1226128
GGCGCGC3601200.043.1176457
ATCCATA1845000.039.90735216-17
TCCATAT1862450.039.5500218-19
Produced by FastQC (version 0.11.5)