##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051278_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29830305 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.989139534443243 33.0 31.0 34.0 30.0 34.0 2 32.285509417352586 34.0 31.0 34.0 30.0 34.0 3 32.358464320093276 34.0 31.0 34.0 30.0 34.0 4 35.826819839756915 37.0 35.0 37.0 35.0 37.0 5 35.83495911288872 37.0 35.0 37.0 35.0 37.0 6 35.828740671608955 37.0 35.0 37.0 35.0 37.0 7 35.77214232305033 37.0 35.0 37.0 35.0 37.0 8 35.78982947039931 37.0 35.0 37.0 35.0 37.0 9 37.47445471978915 39.0 37.0 39.0 35.0 39.0 10-11 37.440154500599306 39.0 37.0 39.0 34.5 39.0 12-13 37.37367244485097 39.0 37.0 39.0 34.0 39.0 14-15 38.73107521026017 40.0 38.0 41.0 34.5 41.0 16-17 38.69066162749593 40.0 38.0 41.0 34.5 41.0 18-19 38.67910889613767 40.0 38.0 41.0 34.0 41.0 20-21 38.68126417078203 40.0 38.0 41.0 34.0 41.0 22-23 38.66078055521055 40.0 38.0 41.0 34.0 41.0 24-25 38.604563144761684 40.0 38.0 41.0 34.0 41.0 26-27 38.52443597207605 40.0 38.0 41.0 34.0 41.0 28-29 38.314313866385206 40.0 38.0 41.0 34.0 41.0 30-31 37.88046535226509 39.5 37.0 41.0 33.0 41.0 32-33 37.594741387994524 39.0 36.0 41.0 33.0 41.0 34-35 37.440144963318346 39.0 35.0 41.0 33.0 41.0 36-37 37.50579804665088 39.0 35.0 41.0 33.0 41.0 38-39 37.37868923901381 40.0 35.0 41.0 32.5 41.0 40-41 36.88119415808856 40.0 35.0 41.0 30.0 41.0 42-43 36.903707102558954 40.0 35.0 41.0 30.0 41.0 44-45 36.865230140959 40.0 35.0 41.0 30.0 41.0 46-47 36.948778649765735 40.0 35.0 41.0 30.0 41.0 48-49 36.898375863069454 40.0 35.0 41.0 30.0 41.0 50-51 36.74820063019805 39.5 35.0 40.5 30.0 41.0 52-53 36.58356141514477 39.0 35.0 41.0 30.0 41.0 54-55 36.34058644388651 39.0 35.0 41.0 29.5 41.0 56-57 35.9906493245711 39.0 35.0 41.0 28.5 41.0 58-59 35.64754694931882 39.0 35.0 41.0 28.0 41.0 60-61 35.39988258584684 38.5 35.0 41.0 27.5 41.0 62-63 35.07555546280871 38.0 35.0 40.0 26.5 41.0 64-65 34.72985286271796 37.5 34.0 40.0 26.0 41.0 66-67 34.28757833686247 37.0 34.0 40.0 25.5 41.0 68-69 33.87030251618279 36.5 34.0 39.0 25.0 41.0 70-71 33.40679310855185 36.0 34.0 39.0 23.5 41.0 72-73 32.99458912672867 35.5 33.0 39.0 22.5 40.5 74-75 32.58139134011536 35.0 33.0 37.5 21.5 39.5 76-77 32.13069688694098 35.0 33.0 37.0 19.5 39.0 78-79 31.79267789920351 35.0 33.0 36.5 19.0 39.0 80-81 31.465102569350197 35.0 33.0 36.0 18.0 38.0 82-83 31.159944207744438 35.0 32.0 36.0 17.5 37.0 84-85 30.846504251297464 35.0 32.0 35.0 11.5 37.0 86-87 30.573046185749696 35.0 32.0 35.0 8.5 36.0 88-89 30.30536895281493 35.0 32.0 35.0 4.5 36.0 90-91 30.09438285998081 35.0 31.5 35.0 2.0 36.0 92-93 29.89309001366228 35.0 31.0 35.0 2.0 35.5 94-95 29.64942383257563 35.0 31.0 35.0 2.0 35.0 96-97 29.384282309550642 34.0 31.0 35.0 2.0 35.0 98-99 29.10205725352121 34.0 31.0 35.0 2.0 35.0 100-101 27.85503104979986 33.0 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42927430886630447 1101 2 0.3968676983221684 1101 3 0.42613061165997834 1101 4 0.35297987969993017 1101 5 0.3492842236308036 1101 6 0.36164060112542984 1101 7 0.37862143067799536 1101 8 0.28534309849746364 1101 9 0.4113161825455762 1101 10-11 0.3797990587819555 1101 12-13 0.41315280469429183 1101 14-15 0.5447905895299243 1101 16-17 0.4723369068609031 1101 18-19 0.5533498104765755 1101 20-21 0.527945558503518 1101 22-23 0.5151140463040988 1101 24-25 0.5741104107085278 1101 26-27 0.6071011378609938 1101 28-29 0.644951995464055 1101 30-31 0.6433634505777448 1101 32-33 0.520258234826187 1101 34-35 0.4916930734886549 1101 36-37 0.5689685816391261 1101 38-39 0.5874944505878119 1101 40-41 0.4848446411253917 1101 42-43 0.4371149085751327 1101 44-45 0.4524025146331354 1101 46-47 0.3951745392494246 1101 48-49 0.4062039982201924 1101 50-51 0.3183128761029934 1101 52-53 0.26379843940804903 1101 54-55 0.3035670185171284 1101 56-57 0.24468855032313286 1101 58-59 0.1773246297148532 1101 60-61 0.1677929986948996 1101 62-63 0.1702356854985112 1101 64-65 0.10848622549112008 1101 66-67 0.07380435186097145 1101 68-69 -0.01570629775613952 1101 70-71 0.04246742225508626 1101 72-73 0.05677612873834903 1101 74-75 -0.014376375601969471 1101 76-77 -0.09394175639729241 1101 78-79 -0.16754005584338216 1101 80-81 -0.17251022581677944 1101 82-83 -0.1537526262454314 1101 84-85 -0.16082274782015205 1101 86-87 -0.18360952085534166 1101 88-89 -0.1791312766902493 1101 90-91 -0.15348251887416708 1101 92-93 -0.17044152559812886 1101 94-95 -0.1326176067318272 1101 96-97 -0.15462160372141298 1101 98-99 -0.1080358256091003 1101 100-101 0.10957785716953339 1102 1 0.3992707022493107 1102 2 0.373171696773106 1102 3 0.40473734202611666 1102 4 0.29818525057678613 1102 5 0.29065628137937694 1102 6 0.3052291533399938 1102 7 0.3435605919683553 1102 8 0.24533906301324038 1102 9 0.35051492291792385 1102 10-11 0.34563333888094405 1102 12-13 0.3826925016064422 1102 14-15 0.5309101189243677 1102 16-17 0.4670082774816464 1102 18-19 0.5337037582265722 1102 20-21 0.4514518525474358 1102 22-23 0.46253742476383763 1102 24-25 0.5240124380674729 1102 26-27 0.557423674559061 1102 28-29 0.5733950996743999 1102 30-31 0.542406400930517 1102 32-33 0.4569730697866845 1102 34-35 0.44804731290688693 1102 36-37 0.526574059296216 1102 38-39 0.5356968770529988 1102 40-41 0.4567009936923654 1102 42-43 0.4335199354337007 1102 44-45 0.39541633777455587 1102 46-47 0.3269448364956773 1102 48-49 0.3393339411623515 1102 50-51 0.27732404957099277 1102 52-53 0.22958923745928672 1102 54-55 0.21561936346689947 1102 56-57 0.1668549669524566 1102 58-59 0.14873805488289094 1102 60-61 0.07443662266805973 1102 62-63 0.14881822204471717 1102 64-65 0.05211963717131596 1102 66-67 0.04690372047421221 1102 68-69 0.011641413711032556 1102 70-71 0.032313528023614424 1102 72-73 0.00945630583950674 1102 74-75 0.012786085561771188 1102 76-77 -0.11442602579766259 1102 78-79 -0.20720667647355384 1102 80-81 -0.1443738455763217 1102 82-83 -0.10639297495814759 1102 84-85 -0.17811840789097033 1102 86-87 -0.19980328747091747 1102 88-89 -0.11039514013135943 1102 90-91 -0.07828184280361583 1102 92-93 -0.1317908039189959 1102 94-95 -0.08802622688262218 1102 96-97 -0.11533175430546194 1102 98-99 -0.09135434508540996 1102 100-101 0.10179648208076486 1103 1 0.3177117065481596 1103 2 0.30821385393200984 1103 3 0.3581702663440609 1103 4 0.2502428506844936 1103 5 0.2648645885374492 1103 6 0.28065650913715956 1103 7 0.30912978710340866 1103 8 0.2513803652357396 1103 9 0.34467208234053004 1103 10-11 0.3258082380346963 1103 12-13 0.3546445684869681 1103 14-15 0.4549673362671314 1103 16-17 0.4712510299468562 1103 18-19 0.4979103753801084 1103 20-21 0.45537065878731653 1103 22-23 0.45455558551005737 1103 24-25 0.5080105987456278 1103 26-27 0.4972925250239584 1103 28-29 0.48359437939791405 1103 30-31 0.46851610896010953 1103 32-33 0.36401811821561836 1103 34-35 0.3289146373641003 1103 36-37 0.31871883451653105 1103 38-39 0.34954208268589326 1103 40-41 0.308822662935043 1103 42-43 0.2881019005675327 1103 44-45 0.2662757255693009 1103 46-47 0.2277654725787457 1103 48-49 0.24173519618869932 1103 50-51 0.15618184557269643 1103 52-53 0.14216515236090288 1103 54-55 0.09474947013886492 1103 56-57 0.061666462588348736 1103 58-59 0.07159349902224221 1103 60-61 0.029967753427015964 1103 62-63 0.064551446571528 1103 64-65 0.03249990841767669 1103 66-67 -0.024059684976236895 1103 68-69 -0.09839566920596354 1103 70-71 -0.09676492396685177 1103 72-73 -0.1020219581974402 1103 74-75 -0.1696697738582742 1103 76-77 -0.16984265461164227 1103 78-79 -0.24305580193440335 1103 80-81 -0.20956430935439485 1103 82-83 -0.18406701802995684 1103 84-85 -0.2606307359100555 1103 86-87 -0.24378489877999954 1103 88-89 -0.18205708823485622 1103 90-91 -0.19090586197483006 1103 92-93 -0.25532552144782983 1103 94-95 -0.19710048137990555 1103 96-97 -0.2421323449553121 1103 98-99 -0.21218913714489318 1103 100-101 -4.647443546517138E-4 1104 1 0.29161614390256574 1104 2 0.271175776108457 1104 3 0.23552370725783334 1104 4 0.19053026531888406 1104 5 0.18770991417134297 1104 6 0.21798928738554224 1104 7 0.22567412864952985 1104 8 0.18175442121617635 1104 9 0.2309786628486563 1104 10-11 0.2137319030478011 1104 12-13 0.2658510783825463 1104 14-15 0.35004240292525424 1104 16-17 0.35231536008576825 1104 18-19 0.355130313313083 1104 20-21 0.385267906661106 1104 22-23 0.4124921643542976 1104 24-25 0.42933409413782186 1104 26-27 0.39731405259630037 1104 28-29 0.40364492794228113 1104 30-31 0.41919866789594096 1104 32-33 0.3413646278917355 1104 34-35 0.29200786406740775 1104 36-37 0.34337217238304873 1104 38-39 0.3612907033598134 1104 40-41 0.2936028349487998 1104 42-43 0.25732486239261476 1104 44-45 0.23090126872138939 1104 46-47 0.20321480327288555 1104 48-49 0.17010512970237102 1104 50-51 0.07238807998346175 1104 52-53 0.0493450095543011 1104 54-55 0.04294604920529821 1104 56-57 0.011912210900540288 1104 58-59 0.031806944028176076 1104 60-61 -0.027294465793744394 1104 62-63 0.09321282801084152 1104 64-65 0.010161654393712638 1104 66-67 0.06026234625402083 1104 68-69 0.016431611637287347 1104 70-71 -0.0010826831569303863 1104 72-73 -0.049951402314690085 1104 74-75 -0.05651410473245022 1104 76-77 -0.02606429210461414 1104 78-79 -0.13559244412641647 1104 80-81 -0.11796093186667633 1104 82-83 -0.11533001000208642 1104 84-85 -0.15414268943079534 1104 86-87 -0.15857911303456262 1104 88-89 -0.1677999533156047 1104 90-91 -0.11612599438826621 1104 92-93 -0.15871214747330242 1104 94-95 -0.07373920950141866 1104 96-97 -0.09524874626894686 1104 98-99 -0.08173104679598353 1104 100-101 0.09104084392201273 1105 1 0.16772636860647339 1105 2 0.17293041325098102 1105 3 0.1905975532350439 1105 4 0.0521363515813249 1105 5 0.1415625080759284 1105 6 0.14630574106765692 1105 7 0.15102471497602465 1105 8 0.12550041508056609 1105 9 0.20213103470740634 1105 10-11 0.12431463111272478 1105 12-13 0.19104738061790982 1105 14-15 0.28726424638556125 1105 16-17 0.1750431429450856 1105 18-19 0.2622479917130036 1105 20-21 0.19210141661428537 1105 22-23 0.20146708607895647 1105 24-25 0.2457035608868523 1105 26-27 0.2711255296496802 1105 28-29 0.31751730213747464 1105 30-31 0.2973584430296867 1105 32-33 0.12850100405094622 1105 34-35 0.12770474457310144 1105 36-37 0.16665483190186592 1105 38-39 0.1961781583988511 1105 40-41 0.12608402999875068 1105 42-43 0.07873188484203553 1105 44-45 -0.012949669038142986 1105 46-47 -0.0035098555703996226 1105 48-49 0.02258793693960115 1105 50-51 -0.0643118525900519 1105 52-53 -0.14930102920679644 1105 54-55 -0.11531376589374531 1105 56-57 -0.07458472017374618 1105 58-59 -0.11358456646058812 1105 60-61 -0.2055962992278637 1105 62-63 -0.1288466585895378 1105 64-65 -0.22774191343408745 1105 66-67 -0.25278267807910737 1105 68-69 -0.2849416763830419 1105 70-71 -0.3163916703055918 1105 72-73 -0.26843046095405754 1105 74-75 -0.31797343920209187 1105 76-77 -0.24711978736615947 1105 78-79 -0.3086518017416289 1105 80-81 -0.3514839884033023 1105 82-83 -0.36100106669362475 1105 84-85 -0.42605336471252286 1105 86-87 -0.41775802215903113 1105 88-89 -0.3811774993094126 1105 90-91 -0.3141838872750604 1105 92-93 -0.3567097355347464 1105 94-95 -0.33211391783439126 1105 96-97 -0.42397651159605587 1105 98-99 -0.43122271033153226 1105 100-101 -0.25040695065055374 1106 1 0.04741680458513997 1106 2 -0.015988410918822638 1106 3 0.003075455048062281 1106 4 -0.13905624555378182 1106 5 -0.016635599877965035 1106 6 0.0023606416580506107 1106 7 -0.011267932681683135 1106 8 -0.038415199071586414 1106 9 -0.00939157569506932 1106 10-11 -0.023163876732333222 1106 12-13 -0.0360898376084009 1106 14-15 -0.08902843644426639 1106 16-17 -0.09292886825100766 1106 18-19 -0.09168581159229205 1106 20-21 -0.15669943782119589 1106 22-23 -0.021723875737471587 1106 24-25 0.07419522769687603 1106 26-27 -0.19342380897569456 1106 28-29 -0.08080942017144821 1106 30-31 -0.09927306839434635 1106 32-33 -0.22765514603898396 1106 34-35 -0.22050071484311218 1106 36-37 -0.2943020723460066 1106 38-39 -0.13671239861577789 1106 40-41 -0.14612889991933287 1106 42-43 -0.20343417089068083 1106 44-45 -0.23247361179283388 1106 46-47 -0.2014981555416142 1106 48-49 -0.15509882306345446 1106 50-51 -0.37207615083711687 1106 52-53 -0.42895598190531814 1106 54-55 -0.41802373559947625 1106 56-57 -0.38803789524521903 1106 58-59 -0.384658401728899 1106 60-61 -0.38447273114744007 1106 62-63 -0.32464740155634786 1106 64-65 -0.3989608768112376 1106 66-67 -0.4234414474615278 1106 68-69 -0.3996060909042285 1106 70-71 -0.42048720822646146 1106 72-73 -0.4807509389495337 1106 74-75 -0.45536410487109436 1106 76-77 -0.4173092700376273 1106 78-79 -0.6107139601231459 1106 80-81 -0.6188558075559136 1106 82-83 -0.5521551654177763 1106 84-85 -0.6262858032866312 1106 86-87 -0.6206543245040876 1106 88-89 -0.5876386950516235 1106 90-91 -0.5831491946343483 1106 92-93 -0.6831956822042962 1106 94-95 -0.664902047770461 1106 96-97 -0.7989074795354902 1106 98-99 -0.7228326294155671 1106 100-101 -0.5410693670265871 1107 1 -0.045386326498327634 1107 2 -0.1309762060077091 1107 3 -0.11897043119417816 1107 4 -0.10288594825194508 1107 5 -0.17032724617434525 1107 6 -0.08373284215457488 1107 7 -0.10434588913970799 1107 8 -0.1558532300793729 1107 9 -0.09515362379785586 1107 10-11 -0.1388446931988483 1107 12-13 -0.06965087209171372 1107 14-15 -0.088612743948417 1107 16-17 -0.14015677206825927 1107 18-19 -0.1385074622248439 1107 20-21 -0.14454785098911316 1107 22-23 -0.10584068798179658 1107 24-25 -0.14793871607686526 1107 26-27 -0.10375392384068505 1107 28-29 -0.11971722953755659 1107 30-31 -0.06095382067017141 1107 32-33 -0.2301836325478206 1107 34-35 -0.4048752984344546 1107 36-37 -0.2565946973104545 1107 38-39 -0.2104452148924878 1107 40-41 -0.23070419815233834 1107 42-43 -0.25092188784757496 1107 44-45 -0.262087807969003 1107 46-47 -0.2732037669611955 1107 48-49 -0.28644167429236944 1107 50-51 -0.3728790676415059 1107 52-53 -0.28776556618403504 1107 54-55 -0.3039687158214406 1107 56-57 -0.3570209123938213 1107 58-59 -0.42193692767496316 1107 60-61 -0.41333954609915224 1107 62-63 -0.42212496755803386 1107 64-65 -0.5267704250586434 1107 66-67 -0.48052166657537043 1107 68-69 -0.4960151306360814 1107 70-71 -0.46008969201772487 1107 72-73 -0.46148437437622647 1107 74-75 -0.5297777537094888 1107 76-77 -0.7816133912737087 1107 78-79 -0.7998010982992909 1107 80-81 -0.693965860206653 1107 82-83 -0.6649082171858076 1107 84-85 -0.7840973477577577 1107 86-87 -0.7988970030413185 1107 88-89 -0.7395069894590378 1107 90-91 -0.7065473267311404 1107 92-93 -0.7812136576419881 1107 94-95 -0.7841918679612121 1107 96-97 -0.8123643062601964 1107 98-99 -0.8723613792804414 1107 100-101 -0.7914927149362967 1108 1 -0.260291053587661 1108 2 -0.21697537876783457 1108 3 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2308 92-93 -2.213736551520391 2308 94-95 -3.032042178812496 2308 96-97 -2.464881520845818 2308 98-99 -3.0060604871959953 2308 100-101 -3.010231913537204 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 329.0 11 3071.0 12 9616.0 13 19153.0 14 30486.0 15 43265.0 16 58611.0 17 88769.0 18 163751.0 19 352194.0 20 435140.0 21 389882.0 22 363430.0 23 326672.0 24 318712.0 25 324801.0 26 349658.0 27 386014.0 28 427584.0 29 478248.0 30 541694.0 31 634470.0 32 778959.0 33 999304.0 34 1382192.0 35 2061819.0 36 3412920.0 37 6403022.0 38 7522568.0 39 1523012.0 40 957.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 23.95114632585889 32.1250721372108 23.08779947104128 20.835982065889034 2 21.423968008372693 25.93660038004975 34.47407929620566 18.1653523153719 3 20.467078697318048 29.78487816333088 23.93245392563033 25.815589213720745 4 26.348812055391317 24.10331707972815 29.126738730965034 20.421132133915492 5 21.24990341198322 29.826892484002425 23.546651634973227 25.37655246904113 6 21.56845530074198 24.156243122556074 28.15869633247129 26.116605244230655 7 26.613090278493633 24.47438267895685 23.35127649549678 25.561250547052737 8 31.500446659879834 24.115912462709062 23.67176345236039 20.711877425050716 9 20.788030829721656 28.926023384608367 23.952524119347757 26.33342166632222 10-11 28.55723734638315 23.989509996629266 23.84646083906953 23.606791817918054 12-13 25.98826347864239 24.009788837552577 23.935227076465594 26.066720607339434 14-15 25.788594853455237 24.111079320174568 24.008147083980536 26.09217874238966 16-17 25.828118418500917 26.69572436486989 24.00025410400598 23.47590311262322 18-19 23.050905781888588 24.17151450513161 26.860625461254923 25.91695425172488 20-21 22.814156610198925 29.108691647638196 24.555519965350673 23.521631776812203 22-23 20.22147778911412 26.38587671161928 30.05630180449043 23.33634369477617 24-25 20.24987173278986 23.811244973861314 32.832396450522374 23.10648684282645 26-27 20.26024180685706 23.80498376222924 35.4656493429641 20.469125087949596 28-29 20.28866282124839 23.74276092718462 35.54641496290433 20.422161288662654 30-31 20.297071549454138 23.693708439571893 35.528688580577416 20.48053143039655 32-33 20.318033536127217 23.7164834132948 35.50190853344317 20.463574517134813 34-35 20.370096627744825 23.745860018373786 35.46334664806414 20.42069670581725 36-37 20.36775725340075 23.7160597288196 35.41997755508676 20.49620546269289 38-39 20.51858258973189 23.812143925027897 34.91021879216809 20.759054693072134 40-41 21.192308770222823 24.436314468711416 32.79126777961696 21.580108981448802 42-43 21.2102434402811 24.65300896754681 32.92378372174723 21.212963870424854 44-45 21.305049662331612 25.101777570007577 32.43605013802807 21.15712262963273 46-47 21.12221338958183 25.46704555576292 32.34177940183935 21.068961652815908 48-49 21.12363687830625 25.587470929669294 32.08367535201357 21.205216840010884 50-51 21.155396654939704 25.74591925396199 31.8609315589019 21.237752532196406 52-53 21.181691603504646 25.815855957670387 31.71246009624304 21.28999234258193 54-55 21.32678542697498 25.76156177851527 31.44178919505274 21.46986359945701 56-57 21.42135154166208 25.79893836150854 31.21031447717347 21.569395619655918 58-59 21.55612742397714 25.910783598355074 30.901519775780915 21.631569201886865 60-61 21.674574148663567 26.030867657651623 30.460533150529695 21.83402504315511 62-63 21.84999449385449 26.192799570772074 30.03604220607198 21.92116372930146 64-65 21.93994700925698 26.38191306427998 29.670813450797993 22.00732647566504 66-67 22.0567956856208 26.45142216835007 29.288884983835562 22.202897162193576 68-69 22.158462393668422 26.556384760561937 29.011582823509958 22.273570022259683 70-71 22.23302378247441 26.693117750014217 28.758854128113615 22.315004339397763 72-73 22.30137367643799 26.725080868979983 28.54644319298611 22.427102261595913 74-75 22.389423576639743 26.766780636428077 28.409564073247388 22.434231713684788 76-77 22.444888751456833 26.839303278498882 28.236058800085384 22.4797491699589 78-79 22.476486397812632 26.801549118023395 28.144083752018638 22.577880732145335 80-81 22.500526124189186 26.84055310755665 28.058882514737306 22.600038253516857 82-83 22.54324101512262 26.920662988670607 27.944155704664148 22.591940291542624 84-85 22.554887977988656 26.93446463007552 27.81085029119102 22.6997971007448 86-87 22.588593904729233 26.98372600596152 27.74740782561913 22.680272263690124 88-89 22.6374636623418 27.031830103163756 27.65843968234112 22.67226655215332 90-91 22.671348865052078 26.981216524901647 27.564729132112515 22.782705477933764 92-93 22.666591821064078 26.973469608425138 27.58593247063887 22.77400609987191 94-95 22.698325182166354 27.024780081748457 27.519347868202015 22.75754686788318 96-97 22.743595183554767 27.03636377564193 27.438663952739162 22.78137708806414 98-99 22.795071740836878 27.06562157051951 27.396941872014853 22.74236481662876 100-101 22.894708215255665 27.181784491236176 27.078248807127114 22.84525848638104 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15483.0 1 9229.0 2 2428.0 3 1646.5 4 1355.5 5 1229.5 6 1166.5 7 1152.5 8 1172.5 9 1206.0 10 1345.5 11 1508.0 12 2097.5 13 3856.5 14 6342.0 15 10200.5 16 133987.0 17 170192.0 18 80655.0 19 70227.5 20 64536.5 21 69469.5 22 80713.0 23 96138.0 24 116122.0 25 143369.5 26 178361.5 27 218227.0 28 262455.0 29 311562.5 30 368119.5 31 427856.5 32 483860.5 33 534924.5 34 578598.0 35 629066.5 36 694738.5 37 766860.5 38 839762.5 39 909708.0 40 971306.5 41 1012881.5 42 1054436.0 43 1098326.5 44 1125549.0 45 1146627.0 46 1165223.0 47 1169621.5 48 1154281.5 49 1130707.5 50 1101672.0 51 1069183.5 52 1040842.5 53 997573.0 54 931661.0 55 849137.5 56 754632.5 57 661162.5 58 575284.0 59 492652.5 60 405504.5 61 332785.0 62 284950.5 63 240710.0 64 191789.0 65 146905.0 66 113155.0 67 86651.0 68 62663.5 69 45394.0 70 33685.5 71 27240.5 72 23134.0 73 17507.5 74 12359.0 75 7913.0 76 4731.5 77 2952.0 78 1862.0 79 1102.5 80 600.5 81 327.5 82 166.0 83 88.0 84 50.5 85 31.5 86 16.0 87 4.5 88 5.0 89 5.5 90 5.5 91 5.5 92 3.5 93 2.5 94 3.5 95 3.0 96 2.0 97 1.5 98 1.5 99 3.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.3522955933571583E-6 9 0.0 10-11 0.0 12-13 3.3522955933571583E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.1846808136893E-5 28-29 0.0 30-31 0.002086804006864831 32-33 0.004188693343899769 34-35 0.0010442400773307547 36-37 0.00128560536005247 38-39 0.0021773159878854744 40-41 0.0021488214753419385 42-43 0.001553789007521043 44-45 0.0047250606388369145 46-47 9.7887031326029E-4 48-49 2.1119462238150096E-4 50-51 0.0020733948244914023 52-53 0.0013643843064963634 54-55 3.6372407187925163E-4 56-57 0.0 58-59 5.531287729039311E-5 60-61 3.955708800161447E-4 62-63 0.0 64-65 1.6761477966785791E-6 66-67 3.6875251526928736E-5 68-69 0.004242330073393484 70-71 0.004294290655090519 72-73 0.0019208653749936517 74-75 0.003022094477411478 76-77 0.02374430968774875 78-79 0.042118241834939334 80-81 0.03628692365029456 82-83 9.822226088536472E-4 84-85 7.710279864721465E-4 86-87 0.0015873119634546144 88-89 0.0029684577479177636 90-91 5.196058169703595E-5 92-93 2.178992135682153E-5 94-95 5.363672949371453E-5 96-97 0.004057953815758839 98-99 8.665684108828253E-4 100-101 3.352295593357158E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.9830305E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.69508472791428 #Duplication Level Percentage of deduplicated Percentage of total 1 71.48951086601966 31.95229745313897 2 13.522765967479982 12.088023413445473 3 5.512374731595243 7.391281670419894 4 2.821268368412054 5.043873150654449 5 1.6775084436662466 3.7488191010727254 6 1.0685415451807032 2.8655132938288785 7 0.7439998468785551 2.3277195335654595 8 0.5298552744079574 1.8945541106556747 9 0.3979846644163146 1.6009162466847942 >10 2.0234882602184983 16.284060606138272 >50 0.16003907593367772 4.858140717168854 >100 0.05126404796992323 3.5054370080206265 >500 6.416423406765295E-4 0.19913197831999993 >1k 5.689256930503346E-4 0.5185845055691896 >5k 6.519454192635591E-5 0.188799711322226 >10k+ 1.2314524549697652E-4 5.5328474999944355 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 1221771 4.0957375393915685 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 128563 0.4309811783687763 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 101215 0.33930259848164473 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62433 0.20929387078006742 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.548353763060754E-4 3.3522955933571583E-6 0.0 0.0 0.0 2 8.581876718994325E-4 6.704591186714317E-6 0.0 0.0 0.0 3 8.615399674927896E-4 6.704591186714317E-6 0.0 0.0 0.0 4 8.648922630861468E-4 6.704591186714317E-6 0.0 0.0 0.0 5 8.715968542728611E-4 6.704591186714317E-6 0.0 0.0 3.3522955933571583E-6 6 8.850060366462898E-4 6.704591186714317E-6 0.0 0.0 6.704591186714317E-6 7 9.017675146130755E-4 6.704591186714317E-6 0.0 1.0056886780071474E-5 6.704591186714317E-6 8 9.218812881732185E-4 6.704591186714317E-6 0.0 1.0056886780071474E-5 6.704591186714317E-6 9 9.922794956337187E-4 6.704591186714317E-6 0.0 1.0056886780071474E-5 6.704591186714317E-6 10-11 0.001035859338347362 6.704591186714317E-6 0.0 1.0056886780071474E-5 8.380738983392895E-6 12-13 0.001082791476654362 1.1733034576750054E-5 0.0 1.0056886780071474E-5 1.0056886780071474E-5 14-15 0.0011448089451314694 1.3409182373428633E-5 0.0 1.5085330170107213E-5 1.0056886780071474E-5 16-17 0.0012118548569986128 1.5085330170107213E-5 0.0 2.178992135682153E-5 1.0056886780071474E-5 18-19 0.0012789007688657557 2.011377356014295E-5 0.0 2.5142216950178685E-5 1.1733034576750054E-5 20-21 0.0013560035675129704 2.011377356014295E-5 0.0 3.017066034021442E-5 1.3409182373428633E-5 22-23 0.0014297540705668279 2.011377356014295E-5 0.0 3.519910373025016E-5 1.3409182373428633E-5 24-25 0.0015688743376911498 2.178992135682153E-5 0.0 4.02275471202859E-5 1.3409182373428633E-5 26-27 0.0016459771363383645 2.6818364746857266E-5 0.0 4.1903694916964475E-5 1.3409182373428633E-5 28-29 0.0017063184570187935 2.8494512543535844E-5 0.0 4.357984271364305E-5 1.3409182373428633E-5 30-31 0.0017867735512593652 3.017066034021442E-5 0.0 4.357984271364305E-5 1.5085330170107213E-5 32-33 0.0018471148719397942 3.352295593357158E-5 0.0 4.357984271364305E-5 1.676147796678579E-5 34-35 0.0019074561926202228 3.352295593357158E-5 0.0 4.6932138307000215E-5 1.676147796678579E-5 36-37 0.002002996617030902 3.352295593357158E-5 0.0 4.860828610367879E-5 2.011377356014295E-5 38-39 0.002068366381101367 3.352295593357158E-5 0.0 5.363672949371453E-5 2.3466069153500108E-5 40-41 0.0021437930319519027 3.352295593357158E-5 0.0 5.698902508707169E-5 2.3466069153500108E-5 42-43 0.0022192196828024384 3.352295593357158E-5 0.0 6.034132068042884E-5 2.3466069153500108E-5 44-45 0.002304703220433046 3.6875251526928736E-5 0.0 6.201746847710742E-5 2.3466069153500108E-5 46-47 0.002408624383827118 3.6875251526928736E-5 0.0 6.872205966382175E-5 2.3466069153500108E-5 48-49 0.0025142216950178684 3.6875251526928736E-5 0.0 7.039820746050032E-5 2.3466069153500108E-5 50-51 0.002644961223158798 4.357984271364305E-5 0.0 7.207435525717889E-5 2.3466069153500108E-5 52-53 0.0028092237072332984 4.357984271364305E-5 0.0 7.542665085053606E-5 2.8494512543535844E-5 54-55 0.0030036568516480138 4.357984271364305E-5 0.0 7.877894644389322E-5 3.017066034021442E-5 56-57 0.003251726725556443 4.357984271364305E-5 0.0 8.213124203725037E-5 3.017066034021442E-5 58-59 0.003568518659128695 4.6932138307000215E-5 0.0 8.548353763060754E-5 3.6875251526928736E-5 60-61 0.003974146425924911 4.6932138307000215E-5 0.0 8.71596854272861E-5 4.357984271364305E-5 62-63 0.004503809129675343 4.6932138307000215E-5 0.0 8.71596854272861E-5 4.357984271364305E-5 64-65 0.005135716849023166 4.6932138307000215E-5 0.0 9.051198102064326E-5 4.860828610367879E-5 66-67 0.00592183016566542 4.6932138307000215E-5 0.0 9.386427661400043E-5 5.028443390035737E-5 68-69 0.007138713466054068 4.860828610367879E-5 0.0 9.386427661400043E-5 5.028443390035737E-5 70-71 0.008737758464085433 5.363672949371453E-5 0.0 9.721657220735759E-5 5.028443390035737E-5 72-73 0.010752488115693085 5.363672949371453E-5 0.0 1.039211633940719E-4 5.028443390035737E-5 74-75 0.013404153930038597 5.363672949371453E-5 0.0 1.0559731119075048E-4 5.3636729493714526E-5 76-77 0.016846961504416398 5.8665172883750266E-5 0.0 1.0727345898742907E-4 5.8665172883750266E-5 78-79 0.02151503311816624 6.3693616273786E-5 0.0 1.1397805017414338E-4 6.3693616273786E-5 80-81 0.027591068881126092 6.3693616273786E-5 0.0 1.1397805017414338E-4 6.3693616273786E-5 82-83 0.03539018457907152 6.536976407046458E-5 0.0 1.1397805017414338E-4 6.536976407046458E-5 84-85 0.04542025299439613 6.704591186714316E-5 0.0 1.1733034576750053E-4 6.704591186714316E-5 86-87 0.05783212742880101 6.872205966382175E-5 0.0 1.2235878915753626E-4 6.704591186714316E-5 88-89 0.07373374157589069 7.207435525717889E-5 0.0 1.2906338034425057E-4 6.872205966382175E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 157455 0.0 93.828606 1 TCGACGG 161445 0.0 91.5244 2 TATCTCG 158860 0.0 91.20791 2 ATATCTC 163160 0.0 89.002106 1 CGACGGC 170055 0.0 86.8346 3 ACGGCGC 170060 0.0 86.77061 5 CGGCGCG 171190 0.0 86.23946 6 GACGGCG 176455 0.0 83.72819 4 ATCTCGA 174100 0.0 83.16937 3 TCTCGAG 176320 0.0 80.77807 4 CTCGAGG 183520 0.0 77.59339 5 TCGAGGG 184995 0.0 76.95932 6 AGGGCGC 193890 0.0 73.63446 9 GAGGGCG 196635 0.0 72.63793 8 CGAGGGC 197865 0.0 72.035164 7 CGCGCCG 359390 0.0 43.222412 9 GCGCGCC 360420 0.0 43.122612 8 GGCGCGC 360120 0.0 43.117645 7 ATCCATA 184500 0.0 39.907352 16-17 TCCATAT 186245 0.0 39.55002 18-19 >>END_MODULE