FastQCFastQC Report
Tue 31 May 2016
SRR1051278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29830305
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1285840.43105157657623683No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA818180.27427812085729597No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT746260.2501684109498713No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336600.11283826967240194No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313110.10496372732360598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG189900.085.5925751
TCGACGG195000.083.648282
ATCTCGA251650.062.7195781
CGACGGC282050.058.033533
ACGGCGC283900.057.655275
CGGCGCG286950.057.0755586
TCTCGAG304750.051.793822
GACGGCG359700.045.7299924
CTCGAGG391900.040.227393
TCGAGGG404700.038.825894
CGCGCCG731200.034.9815149
GGCGCGC734950.034.8418057
GCGCGCC739150.034.6438268
GGGCGCG455050.034.5507938
AGGGCGC494350.031.8809287
GAGGGCG525600.030.0667556
CGAGGGC536750.029.663415
TATCTCG55600.022.4020861
ATCCATA392700.021.94145816-17
TCCATAT414500.020.43796718-19
Produced by FastQC (version 0.11.5)