##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051278_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29830305 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30326387879708 33.0 31.0 34.0 31.0 34.0 2 32.57298720210873 34.0 31.0 34.0 31.0 34.0 3 32.67980803414514 34.0 31.0 34.0 31.0 34.0 4 36.20410813768079 37.0 35.0 37.0 35.0 37.0 5 36.084020428218885 37.0 35.0 37.0 35.0 37.0 6 36.063587046796876 37.0 35.0 37.0 35.0 37.0 7 36.02186984008377 37.0 35.0 37.0 35.0 37.0 8 36.016651489148366 37.0 35.0 37.0 35.0 37.0 9 37.754616085889836 39.0 38.0 39.0 35.0 39.0 10-11 37.72810274651901 39.0 37.0 39.0 35.0 39.0 12-13 37.7181210517291 39.0 37.0 39.0 35.0 39.0 14-15 39.084913463003474 40.0 38.0 41.0 36.0 41.0 16-17 39.072380101376766 40.0 38.0 41.0 36.0 41.0 18-19 39.08265758596836 40.0 38.5 41.0 36.0 41.0 20-21 39.10832405166491 40.0 39.0 41.0 36.0 41.0 22-23 39.051311074425826 40.0 39.0 41.0 35.5 41.0 24-25 38.9902618830079 40.0 38.0 41.0 35.0 41.0 26-27 38.8352859449476 40.0 38.0 41.0 35.0 41.0 28-29 38.64575042058739 40.0 38.0 41.0 34.5 41.0 30-31 38.4940600171537 40.0 38.0 41.0 34.0 41.0 32-33 38.62605487942547 40.0 38.0 41.0 35.0 41.0 34-35 38.60103197067546 40.0 38.0 41.0 35.0 41.0 36-37 38.54770621688246 40.0 38.0 41.0 34.0 41.0 38-39 38.463956620624565 40.0 38.0 41.0 34.0 41.0 40-41 38.32413424871117 40.0 38.0 41.0 34.0 41.0 42-43 38.242227107634335 40.0 38.0 41.0 33.0 41.0 44-45 38.16962945903503 40.0 38.0 41.0 33.0 41.0 46-47 38.05578766291528 40.0 37.5 41.0 33.0 41.0 48-49 37.9883245913845 40.0 37.0 41.0 33.0 41.0 50-51 37.99775428042053 40.0 37.0 41.0 33.0 41.0 52-53 37.85930398633202 40.0 37.0 41.0 33.0 41.0 54-55 37.617426707504336 40.0 36.5 41.0 33.0 41.0 56-57 37.44993125279812 39.5 36.0 41.0 32.0 41.0 58-59 37.219076690633905 39.0 36.0 41.0 32.0 41.0 60-61 36.96405631789551 39.0 35.0 41.0 31.5 41.0 62-63 36.64932805749053 39.0 35.0 40.5 31.0 41.0 64-65 36.31264903258616 38.0 35.0 40.0 31.0 41.0 66-67 35.93047531696374 37.0 35.0 40.0 31.0 41.0 68-69 35.54283818083657 37.0 35.0 39.5 30.0 41.0 70-71 35.14420601800752 36.0 34.0 39.0 30.0 41.0 72-73 34.74437671689914 36.0 34.0 39.0 29.0 40.0 74-75 34.36571610648969 35.0 34.0 37.5 29.0 40.0 76-77 33.83472606129907 35.0 33.5 37.0 29.0 39.0 78-79 33.63516988847415 35.0 34.0 37.0 29.0 39.0 80-81 33.347758143941206 35.0 33.5 36.0 29.0 38.5 82-83 33.034288268256056 35.0 33.0 36.0 29.0 37.0 84-85 32.771075371170355 35.0 33.0 35.5 28.0 37.0 86-87 32.48117458068229 35.0 33.0 35.0 27.0 36.5 88-89 32.28032081804058 35.0 33.0 35.0 27.0 36.0 90-91 32.053635405336955 35.0 33.0 35.0 26.5 36.0 92-93 31.809524223771767 35.0 33.0 35.0 26.0 35.5 94-95 31.566372368636525 35.0 32.0 35.0 25.0 35.0 96-97 31.357901436140192 35.0 32.0 35.0 25.0 35.0 98-99 31.04877489519467 34.5 32.0 35.0 24.5 35.0 100-101 29.49135090975436 33.0 29.5 34.5 12.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4046738661986993 1101 2 0.3991276486864095 1101 3 0.3689730072587807 1101 4 0.23335784974763385 1101 5 0.2974747125032522 1101 6 0.2955796544074616 1101 7 0.3428754702225163 1101 8 0.32854836143413735 1101 9 0.4314144360212566 1101 10-11 0.4179467019499654 1101 12-13 0.4372101386544287 1101 14-15 0.5620297821863147 1101 16-17 0.5019131258012379 1101 18-19 0.4388271257932388 1101 20-21 0.40555149011154157 1101 22-23 0.417554505192669 1101 24-25 0.40518168229635876 1101 26-27 0.39399419193581764 1101 28-29 0.42119536760854004 1101 30-31 0.4140311849305931 1101 32-33 0.4299793371144176 1101 34-35 0.40757870232696547 1101 36-37 0.37919065649783334 1101 38-39 0.353537128870812 1101 40-41 0.3462790838958938 1101 42-43 0.3332957622278556 1101 44-45 0.31269219308825313 1101 46-47 0.29258004534460014 1101 48-49 0.30078723275185837 1101 50-51 0.3789034962931126 1101 52-53 0.3798977844357765 1101 54-55 0.4308192177846877 1101 56-57 0.3514874882303758 1101 58-59 0.33145054166846677 1101 60-61 0.25673808009263155 1101 62-63 0.2713711064724933 1101 64-65 0.27442414691442707 1101 66-67 0.17698941575194027 1101 68-69 0.1531266079763114 1101 70-71 0.15768726333676142 1101 72-73 0.111579998722938 1101 74-75 0.11722401135322968 1101 76-77 0.03793898094140502 1101 78-79 0.09242663174051557 1101 80-81 0.053568883498712694 1101 82-83 -0.1093153725695899 1101 84-85 -0.07382414778732027 1101 86-87 0.04975810882507403 1101 88-89 0.032584670189535814 1101 90-91 -0.03076805893493173 1101 92-93 -0.163709832485754 1101 94-95 -0.13199191244182984 1101 96-97 -0.1315319370110437 1101 98-99 -0.10914353775385521 1101 100-101 -0.1174918141632375 1102 1 0.38269953195305817 1102 2 0.3654559111122069 1102 3 0.340482834169471 1102 4 0.208446004054764 1102 5 0.2670823442784993 1102 6 0.304178613101719 1102 7 0.31749015901738176 1102 8 0.30539911161648803 1102 9 0.4104367292788069 1102 10-11 0.3986920253375459 1102 12-13 0.38229527510316785 1102 14-15 0.4951104357468239 1102 16-17 0.45820423818682343 1102 18-19 0.42186295578267874 1102 20-21 0.37604910966786775 1102 22-23 0.3449199374911558 1102 24-25 0.3477278056742392 1102 26-27 0.35882385215187185 1102 28-29 0.4070114317061382 1102 30-31 0.3627435434629831 1102 32-33 0.33442766922681955 1102 34-35 0.39109340317249064 1102 36-37 0.3744014066001 1102 38-39 0.37280864748110787 1102 40-41 0.34744524834101753 1102 42-43 0.34957424879835486 1102 44-45 0.3167299557409109 1102 46-47 0.26976047918535784 1102 48-49 0.3221856852123537 1102 50-51 0.41220598300457567 1102 52-53 0.426343076837874 1102 54-55 0.4524927423484897 1102 56-57 0.3528401972264419 1102 58-59 0.35652257931081976 1102 60-61 0.30765590747365223 1102 62-63 0.3215024501260828 1102 64-65 0.2979725393139532 1102 66-67 0.2877773595131501 1102 68-69 0.24393895590550585 1102 70-71 0.245669941863234 1102 72-73 0.2593158072229116 1102 74-75 0.20490803095418642 1102 76-77 0.13601286659413603 1102 78-79 0.17470718833664023 1102 80-81 0.21311189479348513 1102 82-83 0.08390835942779518 1102 84-85 0.06515305213537914 1102 86-87 0.19885525269247495 1102 88-89 0.17945520563745276 1102 90-91 0.10403700056843945 1102 92-93 0.0032525549067514703 1102 94-95 0.04798160153394804 1102 96-97 0.015630285643457142 1102 98-99 0.025322261826694614 1102 100-101 0.07408591658946762 1103 1 0.2828085678375487 1103 2 0.2907656823303313 1103 3 0.2617106293523861 1103 4 0.17228160039839224 1103 5 0.2118236794554349 1103 6 0.20971362594536913 1103 7 0.24811026948128756 1103 8 0.2521590481784344 1103 9 0.29770503752772015 1103 10-11 0.28125218145424213 1103 12-13 0.30532030049046455 1103 14-15 0.3964442172142384 1103 16-17 0.37125557500480966 1103 18-19 0.3096614264740438 1103 20-21 0.2889034068566829 1103 22-23 0.22365846739322137 1103 24-25 0.18207297184588356 1103 26-27 0.20018937979094176 1103 28-29 0.2317230634756271 1103 30-31 0.2100419044362667 1103 32-33 0.19500940114935617 1103 34-35 0.20719258823642406 1103 36-37 0.23367135811947293 1103 38-39 0.2123411696113493 1103 40-41 0.15168534861930993 1103 42-43 0.1795599830840473 1103 44-45 0.15491179396759946 1103 46-47 0.18673434916475173 1103 48-49 0.2255544075007947 1103 50-51 0.2543065526731354 1103 52-53 0.2605370629242856 1103 54-55 0.2825606482180234 1103 56-57 0.22395253052251007 1103 58-59 0.18417711017925598 1103 60-61 0.152213869572968 1103 62-63 0.15795125703569823 1103 64-65 0.1411384999805989 1103 66-67 0.15211752460832173 1103 68-69 0.12224330935464423 1103 70-71 0.14399953222022788 1103 72-73 0.15350635100957533 1103 74-75 0.1425955130891623 1103 76-77 0.04769314268808955 1103 78-79 0.10324176208253988 1103 80-81 0.10000853227539608 1103 82-83 0.01226809625599401 1103 84-85 0.0017043391064817115 1103 86-87 0.12094034519230945 1103 88-89 0.06907146642093664 1103 90-91 -0.04011760021597155 1103 92-93 -0.11179162214661176 1103 94-95 -0.06578866647251758 1103 96-97 -0.05710870384486455 1103 98-99 -0.07230595492650949 1103 100-101 -0.06100160378251829 1104 1 0.37087716518946934 1104 2 0.3729512885181734 1104 3 0.3236869139829608 1104 4 0.20446061335307064 1104 5 0.25072986667397856 1104 6 0.245702488421351 1104 7 0.2873424173034138 1104 8 0.29467222592428044 1104 9 0.3731984471647607 1104 10-11 0.3623792545976059 1104 12-13 0.3663966560013847 1104 14-15 0.4871908872400965 1104 16-17 0.4064776386958613 1104 18-19 0.41250581383221885 1104 20-21 0.36785745296909766 1104 22-23 0.3523244852789702 1104 24-25 0.3312492092636319 1104 26-27 0.3699503893024172 1104 28-29 0.3601952212860766 1104 30-31 0.35454332221485174 1104 32-33 0.3473208814418527 1104 34-35 0.3771547737576739 1104 36-37 0.36130354029068457 1104 38-39 0.3384244030757202 1104 40-41 0.31096574744798033 1104 42-43 0.3178641125670154 1104 44-45 0.27125751436463474 1104 46-47 0.2814805637274276 1104 48-49 0.30754649316278204 1104 50-51 0.33392633835062213 1104 52-53 0.3432927796735541 1104 54-55 0.3103799281432984 1104 56-57 0.2063778634272282 1104 58-59 0.251019933543148 1104 60-61 0.153015901162199 1104 62-63 0.14790756203429822 1104 64-65 0.16682159622166637 1104 66-67 0.08633279603322563 1104 68-69 0.054498296755816966 1104 70-71 0.11793717040379903 1104 72-73 0.11377641906174318 1104 74-75 0.15168461974960223 1104 76-77 0.005293810310277536 1104 78-79 0.030569005846579955 1104 80-81 0.06234494960807524 1104 82-83 -0.0636919089114798 1104 84-85 -0.08140743351989244 1104 86-87 0.042324604142706335 1104 88-89 0.054160747223207295 1104 90-91 -0.00714423742041248 1104 92-93 -0.13395969203027036 1104 94-95 -0.06837707268605087 1104 96-97 -0.08653991580965936 1104 98-99 -0.02354107582849707 1104 100-101 -0.002631246741845672 1105 1 -0.015159620416831388 1105 2 0.10576072236924716 1105 3 0.11553006945112543 1105 4 0.08711127255147488 1105 5 0.09395204948053504 1105 6 0.13724427180190446 1105 7 0.15889969353477085 1105 8 0.10217422966004364 1105 9 0.2031047142150868 1105 10-11 0.16719215530977038 1105 12-13 0.1926497011097581 1105 14-15 0.23661839344418212 1105 16-17 0.20080439085965907 1105 18-19 0.13241735142390354 1105 20-21 0.11671257288611514 1105 22-23 0.13843801947392365 1105 24-25 0.06763236386002802 1105 26-27 0.07534635975405024 1105 28-29 0.08262590760163846 1105 30-31 0.09424764943166508 1105 32-33 0.14879677933405588 1105 34-35 0.12857072751987175 1105 36-37 0.11849081730526478 1105 38-39 0.11674009274530306 1105 40-41 0.08989713865257443 1105 42-43 0.08481725244092786 1105 44-45 0.052065704561080395 1105 46-47 -0.07812272954442534 1105 48-49 0.09482349820009972 1105 50-51 0.1002847755832974 1105 52-53 0.11119715712560918 1105 54-55 0.11279932965672401 1105 56-57 -0.032845760277680824 1105 58-59 0.036593986471700646 1105 60-61 0.03421917521223605 1105 62-63 0.08830529582174762 1105 64-65 0.09189538100154238 1105 66-67 0.07592954920519901 1105 68-69 0.033363279304772675 1105 70-71 -0.03491573092517086 1105 72-73 0.01633503752506016 1105 74-75 0.0645183589956062 1105 76-77 -0.07503268175847211 1105 78-79 0.0343646493288432 1105 80-81 0.0433229795977752 1105 82-83 -0.04410580916577089 1105 84-85 -0.08540469265518169 1105 86-87 0.028532836767610092 1105 88-89 -0.005081816040465981 1105 90-91 -0.06597162253774513 1105 92-93 -0.17347235515934756 1105 94-95 -0.11109656101572796 1105 96-97 -0.09815156199671904 1105 98-99 -0.03425278332994708 1105 100-101 0.02763544273959795 1106 1 -0.06704608087022024 1106 2 0.03037964953972505 1106 3 0.04879395105653117 1106 4 0.029489576151149777 1106 5 -0.20848445268392624 1106 6 -0.07394195894132594 1106 7 -0.008738062929388946 1106 8 -0.1451204253982894 1106 9 -0.20281811783253545 1106 10-11 0.08840049157300456 1106 12-13 0.11225925418968075 1106 14-15 0.0011773636089529305 1106 16-17 0.09238654666022228 1106 18-19 -0.01651476871963098 1106 20-21 -0.02680175804248819 1106 22-23 -0.02109058761676863 1106 24-25 -0.006274420497419442 1106 26-27 -0.010905433898642514 1106 28-29 -0.08036716009470979 1106 30-31 -0.11599139570759576 1106 32-33 -0.09140318177578166 1106 34-35 -0.032463873490996775 1106 36-37 -0.040211777558319284 1106 38-39 -0.058940861234944464 1106 40-41 -0.08059842290180086 1106 42-43 -0.1979088990173068 1106 44-45 -0.14270939763163426 1106 46-47 -0.202055281411873 1106 48-49 -0.09886092551261072 1106 50-51 -0.07899939844038073 1106 52-53 -0.1640516714326523 1106 54-55 -0.13443657527400177 1106 56-57 -0.20935956486644614 1106 58-59 -0.16727527030860045 1106 60-61 -0.24438714254117144 1106 62-63 -0.2186151126328113 1106 64-65 -0.24620912266577477 1106 66-67 -0.26956299275909146 1106 68-69 -0.2957268396055355 1106 70-71 -0.28871576852048975 1106 72-73 -0.2575577542868217 1106 74-75 -0.2999139766663603 1106 76-77 -0.4391761472116116 1106 78-79 -0.3356821246384314 1106 80-81 -0.32635825682967834 1106 82-83 -0.43770066469859614 1106 84-85 -0.45247668565479415 1106 86-87 -0.3189708623499854 1106 88-89 -0.29352184764542955 1106 90-91 -0.42407181190566234 1106 92-93 -0.5897388076394208 1106 94-95 -0.5399815720856971 1106 96-97 -0.521000983450989 1106 98-99 -0.5197778531648432 1106 100-101 -0.5826099264779678 1107 1 -0.08357400449677499 1107 2 -0.043793496266445686 1107 3 -0.07490087236936915 1107 4 -0.05284283684130031 1107 5 -0.03329459816493596 1107 6 -0.0258904756724192 1107 7 0.008034364923965143 1107 8 0.0072155115457874786 1107 9 -0.0174734260219509 1107 10-11 -0.029264950468459006 1107 12-13 -0.049790035336442884 1107 14-15 -0.02730689779665596 1107 16-17 -0.04724938728624295 1107 18-19 -0.11742936022684347 1107 20-21 -0.13170625083636622 1107 22-23 -0.18701782846986248 1107 24-25 -0.27558807311694977 1107 26-27 -0.29200680170499993 1107 28-29 -0.27849185632663875 1107 30-31 -0.36500900254568336 1107 32-33 -0.3581983307667329 1107 34-35 -0.27914342032509154 1107 36-37 -0.28253324056685614 1107 38-39 -0.20866150299445962 1107 40-41 -0.28530925736740187 1107 42-43 -0.31913660524399745 1107 44-45 -0.36602372138054307 1107 46-47 -0.33006450092400286 1107 48-49 -0.28209868806088423 1107 50-51 -0.17403381292138675 1107 52-53 -0.20294244351998714 1107 54-55 -0.32709418925651335 1107 56-57 -0.42599238668394435 1107 58-59 -0.3632539260894987 1107 60-61 -0.4168965822199837 1107 62-63 -0.3544196438144738 1107 64-65 -0.3541355419528571 1107 66-67 -0.4206237449183803 1107 68-69 -0.421097354717169 1107 70-71 -0.40310891764446666 1107 72-73 -0.395882311167135 1107 74-75 -0.4260512487262531 1107 76-77 -0.5914649633012843 1107 78-79 -0.5113094463321275 1107 80-81 -0.4906409176050843 1107 82-83 -0.5169667495932231 1107 84-85 -0.5905963681727684 1107 86-87 -0.5196430220099586 1107 88-89 -0.5215616303199582 1107 90-91 -0.6748322930434796 1107 92-93 -0.7859490824550548 1107 94-95 -0.7661695067267722 1107 96-97 -0.8800519738168475 1107 98-99 -0.9127924982983586 1107 100-101 -0.8926640416650926 1108 1 -0.26418355554330475 1108 2 -0.17597310247837328 1108 3 -0.17755705441707192 1108 4 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64-65 -0.673288816745341 2308 66-67 -0.7272848312816365 2308 68-69 -0.6536096888107252 2308 70-71 -0.5863303612806519 2308 72-73 -0.6494631249882943 2308 74-75 -0.5520428996238422 2308 76-77 -1.708470112435144 2308 78-79 -1.129092073966973 2308 80-81 -0.6499164051613917 2308 82-83 -0.6125791807630208 2308 84-85 -0.5683327366705413 2308 86-87 -0.9858307387736609 2308 88-89 -0.9586933057022051 2308 90-91 -1.6332302483845638 2308 92-93 -0.8735218000210168 2308 94-95 -0.7565950017041843 2308 96-97 -0.9365315324266987 2308 98-99 -1.5606904395944667 2308 100-101 -1.0117724250454287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 5.0 11 37.0 12 268.0 13 1661.0 14 6269.0 15 14876.0 16 26236.0 17 41085.0 18 66369.0 19 112249.0 20 124441.0 21 122526.0 22 120334.0 23 123996.0 24 137551.0 25 161417.0 26 199578.0 27 249872.0 28 313967.0 29 392658.0 30 494298.0 31 621058.0 32 794487.0 33 1047745.0 34 1460835.0 35 2202333.0 36 3633982.0 37 6942802.0 38 8604416.0 39 1811532.0 40 1422.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.858750764985665 29.393735438430735 25.730480786781047 23.017033009802553 2 23.730757278467966 28.053567351913888 27.807792193860497 20.40788317575765 3 22.388643101913498 26.898821994767864 27.184478256943002 23.528056646375635 4 23.84012071492052 26.647747636034673 27.114826709141624 22.397304939903183 5 23.65139411078767 27.738610114780926 26.14296434448122 22.46703142995018 6 23.996358736526496 26.812146238531586 25.646656981884696 23.54483804305722 7 24.23581656305559 27.25081423069593 25.945021346580265 22.568347859668215 8 24.03979107823403 26.630233918158062 26.29258064910835 23.03739435449956 9 23.113209871638926 26.262037883957273 26.644544868046104 23.9802073763577 10-11 23.33708958054569 26.61863162310945 26.434505111496513 23.609773684848346 12-13 23.352868835903625 26.661105208277284 26.553288677403735 23.432737278415356 14-15 23.14196586323874 26.735561369553544 26.62552561899719 23.49694714821052 16-17 23.244291333930377 26.981695292756815 26.61635038595817 23.157662987354637 18-19 22.713730214960925 26.860969071553242 27.196051129882846 23.229249583602986 20-21 22.456498517195854 26.74974660835684 27.245210868611636 23.548544005835677 22-23 22.484986325148203 26.67385566456662 27.85068908950143 22.990468920783748 24-25 22.516050960530002 26.331587256474975 28.41754669463286 22.73481508836216 26-27 22.539566725851444 26.336658307717602 28.430993246632912 22.692781719798038 28-29 22.57053355639508 26.399954676963578 28.40003982527165 22.62947194136969 30-31 22.584095268218007 26.35601949091704 28.377502341997506 22.68238289886744 32-33 22.57237229052804 26.38895579512177 28.373459473511918 22.665212440838268 34-35 22.629334497250365 26.431719353858433 28.320461356328742 22.618484792562462 36-37 22.64355828745298 26.40054300484021 28.26743306848522 22.68846563922159 38-39 22.64417846213775 26.45498931371972 28.154519372161968 22.74631285198056 40-41 22.749172360121694 26.57818114833221 27.89267156336484 22.779974928181257 42-43 22.739737324174193 26.59650814834109 27.87441831385901 22.789336213625706 44-45 22.75247101898556 26.671752099081793 27.80808644095325 22.7676904409794 46-47 22.776116435953302 26.766967350819915 27.730532758548733 22.726383454678054 48-49 22.760483005453683 26.748863278467987 27.699659792281707 22.790993923796623 50-51 22.730168196402953 26.778291405334276 27.70712870686371 22.784411691399065 52-53 22.752403973073694 26.82767574786781 27.678282203282865 22.741638075775626 54-55 22.769485595269643 26.80364649305463 27.611816238553377 22.81505167312235 56-57 22.758030801227143 26.89331201943795 27.55819124209424 22.79046593724067 58-59 22.809382941273984 26.940659842398528 27.480969436953462 22.76898777937403 60-61 22.824567164164094 26.936979021837022 27.379160219783206 22.859293594215682 62-63 22.833423929121743 27.002608588815967 27.318522220942764 22.845445261119522 64-65 22.842446632711262 27.08117131219409 27.260279437303776 22.816102617790868 66-67 22.871450694184993 27.07481368360129 27.175778122282026 22.877957499931696 68-69 22.88188136192372 27.118864523845797 27.133395049095206 22.865859065135272 70-71 22.90722974505289 27.17628934736001 27.0815216270836 22.8349592805035 72-73 22.906895283400395 27.176850091772447 27.033341664905592 22.882912959921562 74-75 22.901990107040472 27.22346787939312 27.03877315367711 22.835768859889296 76-77 22.89871826654136 27.32437700519656 26.95115420375353 22.82575052450855 78-79 22.88408717242415 27.31029401140887 26.940725212162597 22.864893604004386 80-81 22.841635346569113 27.367547281184002 26.934671540750344 22.856145831496548 82-83 22.86698647217214 27.45209940498583 26.88198951573059 22.798924607111434 84-85 22.843395332364185 27.44095140830776 26.851917873451175 22.86373538587688 86-87 22.831268403055216 27.519715269421486 26.860372362937625 22.788643964585678 88-89 22.859074018854315 27.58059128124905 26.80712282358494 22.753211876311692 90-91 22.84962557372444 27.57326819152536 26.762205414929547 22.81490081982065 92-93 22.828770414622166 27.612830020318096 26.781150584002607 22.777248981057134 94-95 22.832997131574466 27.699729623924025 26.73404546826244 22.733227776239072 96-97 22.82071111310163 27.718039682062912 26.696067193951496 22.765182010883965 98-99 22.806128448914418 27.78926536869144 26.67752331726553 22.727082865128615 100-101 22.85111044739364 27.868477606315444 26.450493041529104 22.829918904761808 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 71623.0 1 41640.5 2 8932.0 3 5479.5 4 4562.0 5 4251.5 6 4030.0 7 3952.5 8 3899.0 9 3905.0 10 4073.5 11 4215.0 12 5051.5 13 7176.0 14 9920.5 15 12251.0 16 30466.5 17 35159.0 18 23040.0 19 24946.0 20 29956.5 21 39264.5 22 50980.0 23 65127.0 24 83159.5 25 107351.0 26 137764.0 27 170964.5 28 207821.5 29 248691.5 30 295879.0 31 349710.5 32 403812.0 33 457368.5 34 506430.5 35 562350.5 36 635065.0 37 713771.5 38 795509.5 39 877073.5 40 951164.5 41 1003269.5 42 1054013.5 43 1108875.0 44 1148509.5 45 1182973.5 46 1210849.0 47 1224147.0 48 1216944.0 49 1199041.0 50 1176434.0 51 1149528.5 52 1126299.0 53 1086314.0 54 1018886.0 55 930116.5 56 828514.5 57 729930.0 58 638153.5 59 547320.5 60 449950.0 61 368082.5 62 315135.5 63 267817.5 64 214011.5 65 163615.0 66 126305.5 67 96839.0 68 71170.0 69 53109.5 70 40204.5 71 32307.5 72 27420.5 73 21055.5 74 15092.5 75 9914.0 76 6178.5 77 4068.5 78 2537.5 79 1503.5 80 842.5 81 457.5 82 233.5 83 147.0 84 105.0 85 60.5 86 26.5 87 4.5 88 2.5 89 1.5 90 1.5 91 2.5 92 2.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08657303369844861 2 6.536976407046458E-4 3 1.5755789288778642E-4 4 8.04550942405718E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.0056886780071474E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.3522955933571583E-6 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.061966345969309E-4 82-83 3.050588989955014E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 5.028443390035737E-6 94-95 1.0056886780071474E-5 96-97 3.3522955933571583E-6 98-99 1.6426248407450073E-4 100-101 2.8494512543535844E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.9830305E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.124432236422535 #Duplication Level Percentage of deduplicated Percentage of total 1 70.52813424098761 31.82542014318758 2 13.619418358870883 12.291370416687162 3 5.76263318784324 7.801066523645756 4 2.9000481497448787 5.234521048621013 5 1.7701495240252514 3.993849612260653 6 1.1665573608061575 3.1584143146558428 7 0.8057354871867326 2.545084947442703 8 0.5622481360333053 2.0296902331591813 9 0.42946472571539523 1.744141672213033 >10 2.212960030968434 18.080187475296874 >50 0.1832126715500241 5.64963468512187 >100 0.05890479560514133 4.064913687830586 >500 3.6233036044473597E-4 0.10255728856260098 >1k 6.747731441623939E-5 0.044239092165397655 >5k 4.436699495834579E-5 0.138477455771568 >10k+ 5.915599309301958E-5 1.296431403378195 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128584 0.43105157657623683 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 81818 0.27427812085729597 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 74626 0.2501684109498713 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33660 0.11283826967240194 No Hit GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31311 0.10496372732360598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.48130785119361E-4 3.3522955933571583E-6 0.0 0.0 0.0 2 8.48130785119361E-4 3.3522955933571583E-6 0.0 0.0 0.0 3 8.548353763060754E-4 3.3522955933571583E-6 0.0 1.3409182373428633E-5 0.0 4 8.648922630861468E-4 3.3522955933571583E-6 0.0 1.3409182373428633E-5 0.0 5 8.715968542728611E-4 3.3522955933571583E-6 0.0 2.3466069153500108E-5 0.0 6 8.850060366462898E-4 3.3522955933571583E-6 0.0 2.3466069153500108E-5 0.0 7 9.017675146130755E-4 3.3522955933571583E-6 0.0 2.6818364746857266E-5 0.0 8 9.252335837665757E-4 6.704591186714317E-6 0.0 3.352295593357158E-5 3.3522955933571583E-6 9 9.554042441067901E-4 6.704591186714317E-6 0.0 3.352295593357158E-5 6.704591186714317E-6 10-11 0.0010006602346171118 6.704591186714317E-6 0.0 3.6875251526928736E-5 1.0056886780071474E-5 12-13 0.0010526208163141476 8.380738983392895E-6 0.0 4.02275471202859E-5 1.0056886780071474E-5 14-15 0.0011079336936045407 1.1733034576750054E-5 0.0 4.02275471202859E-5 1.0056886780071474E-5 16-17 0.001178331901065041 1.8437625763464368E-5 0.0 4.357984271364305E-5 1.0056886780071474E-5 18-19 0.0012369970739487912 2.011377356014295E-5 0.0 4.6932138307000215E-5 1.0056886780071474E-5 20-21 0.0013140998725960059 2.178992135682153E-5 0.0 5.3636729493714526E-5 1.0056886780071474E-5 22-23 0.0013794696366664705 2.3466069153500108E-5 0.0 6.201746847710742E-5 1.0056886780071474E-5 24-25 0.0015135614604007569 2.6818364746857266E-5 0.0 6.704591186714316E-5 1.8437625763464368E-5 26-27 0.0015873119634546144 2.6818364746857266E-5 0.0 7.375050305385747E-5 2.3466069153500108E-5 28-29 0.0016510055797284004 2.8494512543535844E-5 0.0 7.710279864721464E-5 2.6818364746857263E-5 30-31 0.0017314606739689722 3.017066034021442E-5 0.0 8.04550942405718E-5 3.017066034021442E-5 32-33 0.0017934781424460795 3.352295593357158E-5 0.0 9.218812881732185E-5 3.352295593357158E-5 34-35 0.0018538194631265085 3.519910373025016E-5 0.0 9.554042441067901E-5 4.02275471202859E-5 36-37 0.0019460075919438304 3.6875251526928736E-5 0.0 9.721657220735759E-5 4.1903694916964475E-5 38-39 0.002011377356014295 4.02275471202859E-5 0.0 1.0056886780071474E-4 4.357984271364305E-5 40-41 0.002081775563474795 4.02275471202859E-5 0.0 1.039211633940719E-4 4.525599051032163E-5 42-43 0.0021521737709352955 4.525599051032163E-5 0.0 1.039211633940719E-4 4.6932138307000215E-5 44-45 0.002234305012972546 4.860828610367879E-5 0.0 1.0727345898742907E-4 4.6932138307000215E-5 46-47 0.002336550028569939 5.028443390035737E-5 0.0 1.0727345898742907E-4 4.6932138307000215E-5 48-49 0.002437118896370654 5.363672949371453E-5 0.0 1.0727345898742907E-4 4.6932138307000215E-5 50-51 0.0025661822767149046 5.363672949371453E-5 0.0 1.0727345898742907E-4 4.860828610367879E-5 52-53 0.0027337970563827627 5.363672949371453E-5 0.0 1.1062575458078622E-4 5.531287729039311E-5 54-55 0.00292487790520412 5.363672949371453E-5 0.0 1.1565419797082195E-4 5.698902508707169E-5 56-57 0.0031595385967391216 5.531287729039311E-5 0.0 1.1733034576750053E-4 6.034132068042884E-5 58-59 0.003478006678108051 5.8665172883750266E-5 0.0 1.1733034576750053E-4 6.3693616273786E-5 60-61 0.003873577558124196 6.3693616273786E-5 0.0 1.2571108475089343E-4 6.536976407046458E-5 62-63 0.00438815493170452 6.3693616273786E-5 0.0 1.27387232547572E-4 6.704591186714316E-5 64-65 0.005010005764272273 6.3693616273786E-5 0.0 1.3073952814092915E-4 7.039820746050032E-5 66-67 0.005804499819897919 6.536976407046458E-5 0.0 1.3409182373428632E-4 7.039820746050032E-5 68-69 0.007014678529099853 7.039820746050032E-5 0.0 1.3409182373428632E-4 7.375050305385747E-5 70-71 0.008617075822724574 7.542665085053606E-5 0.0 1.3409182373428632E-4 7.375050305385747E-5 72-73 0.010613367848568762 8.213124203725037E-5 0.0 1.3409182373428632E-4 7.710279864721464E-5 74-75 0.013241567593760774 8.548353763060754E-5 0.0 1.357679715309649E-4 8.213124203725037E-5 76-77 0.01668102287254522 9.051198102064328E-5 0.0 1.441487105143578E-4 8.380738983392895E-5 78-79 0.02130551464358142 9.386427661400043E-5 0.0 1.4582485831103636E-4 8.380738983392895E-5 80-81 0.027311152199080764 9.386427661400043E-5 0.0 1.4917715390439353E-4 8.71596854272861E-5 82-83 0.034996289846852054 9.554042441067901E-5 0.0 1.525294494977507E-4 9.386427661400043E-5 84-85 0.044992835306243095 9.721657220735759E-5 0.0 1.6258633627782218E-4 9.554042441067901E-5 86-87 0.057258884882336936 9.721657220735759E-5 0.0 1.6593863187117932E-4 9.889272000403616E-5 88-89 0.07302808335348901 1.0224501559739333E-4 0.0 1.6929092746453649E-4 1.0056886780071474E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 18990 0.0 85.592575 1 TCGACGG 19500 0.0 83.64828 2 ATCTCGA 25165 0.0 62.719578 1 CGACGGC 28205 0.0 58.03353 3 ACGGCGC 28390 0.0 57.65527 5 CGGCGCG 28695 0.0 57.075558 6 TCTCGAG 30475 0.0 51.79382 2 GACGGCG 35970 0.0 45.729992 4 CTCGAGG 39190 0.0 40.22739 3 TCGAGGG 40470 0.0 38.82589 4 CGCGCCG 73120 0.0 34.981514 9 GGCGCGC 73495 0.0 34.841805 7 GCGCGCC 73915 0.0 34.643826 8 GGGCGCG 45505 0.0 34.550793 8 AGGGCGC 49435 0.0 31.880928 7 GAGGGCG 52560 0.0 30.066755 6 CGAGGGC 53675 0.0 29.66341 5 TATCTCG 5560 0.0 22.402086 1 ATCCATA 39270 0.0 21.941458 16-17 TCCATAT 41450 0.0 20.437967 18-19 >>END_MODULE