FastQCFastQC Report
Tue 31 May 2016
SRR1051277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14291925
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT8626376.035834920768196No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT996000.6968970240188078No Hit
ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC513450.3592588122313824No Hit
ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT488490.3417944048824773No Hit
ATATATGTACATATATGTACATATATGTACATATATGTACATATATGTAC479900.33578401789821877No Hit
ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT440550.30825098788301786No Hit
GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT409310.2863924908645966No Hit
TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG358200.2506310381561616No Hit
ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT340390.2381694558290783No Hit
ACATATATGTACATATATGTACATATATGTACATATATGTACATATATGT329940.2308576346433388No Hit
CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA322510.22565889479548767No Hit
ATATGTACATATATGTACATATATGTACATATATGTACATATATGTACAT302100.2113781033695601No Hit
TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA284250.19888853321018687No Hit
CATATATGTACATATATGTACATATATGTACATATATGTACATATATGTA278690.1949982245218891No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT265080.18547536458524658No Hit
TATATGTACATATATGTACATATATGTACATATATGTACATATATGTACA252250.1764982673782573No Hit
TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA251190.17575658982257464No Hit
GTACATATATGTACATATATGTACATATATGTACATATATGTACATATAT216300.15134420310769894No Hit
ATGTACATATATGTACATATATGTACATATATGTACATATATGTACATAT212110.14841247767533064No Hit
TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA192130.13443255544651964No Hit
TACATATATGTACATATATGTACATATATGTACATATATGTACATATATG176190.1232794042789897No Hit
TATGTACATATATGTACATATATGTACATATATGTACATATATGTACATA169470.1185774484542845No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153210.1072003946284353No Hit
AATTCGAACCTCTGTAACACTCAGACCACGCTGATGCCCAGCGCCTGTTT146650.10261039013288974No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT143000.10005650043643526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG819350.091.671681
TCGACGG841500.090.214222
TATCTCG1258000.090.144712
ATATCTC1256650.090.103941
ACGGCGC857750.088.330635
GACGGCG859850.088.180534
CGGCGCG868150.087.615036
ATCTCGA1299050.087.055693
CGACGGC876650.086.543823
TCTCGAG1327950.084.508134
CTCGAGG1347300.083.13145
TCGAGGG1353750.082.7671056
CGAGGGC1375350.081.7812967
AGGGCGC1387350.081.314389
GAGGGCG1399950.080.555328
AATTCGA58300.068.3601461
TATCGCG13000.066.7018052
TCGCGAG13400.062.623274
ATCGCGA14750.057.839843
AATTCCC108650.052.530361
Produced by FastQC (version 0.11.5)