FastQCFastQC Report
Tue 31 May 2016
SRR1051277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14291925
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC628540.4397868026875316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA582040.4072509476505089No Hit
ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT574920.4022691135029046No Hit
ATATATGTACATATATGTACATATATGTACATATATGTACATATATGTAC563790.3944814991682366No Hit
ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT538850.3770310857354765No Hit
GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT452280.31645841970203453No Hit
CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA432170.3023875370182813No Hit
ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT427100.29884007927553496No Hit
TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG410770.2874140467431784No Hit
ACATATATGTACATATATGTACATATATGTACATATATGTACATATATGT386230.2702435116333174No Hit
CATATATGTACATATATGTACATATATGTACATATATGTACATATATGTA380640.2663322120708022No Hit
ATATGTACATATATGTACATATATGTACATATATGTACATATATGTACAT351280.24578914317000683No Hit
TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA297290.20801256653669814No Hit
TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA294230.20587149736651988No Hit
ATGTACATATATGTACATATATGTACATATATGTACATATATGTACATAT263040.18404798513846105No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT257460.18014368253401833No Hit
TATATGTACATATATGTACATATATGTACATATATGTACATATATGTACA245040.17145346060800068No Hit
TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA228740.16004841894986155No Hit
GTACATATATGTACATATATGTACATATATGTACATATATGTACATATAT216810.1517010479693953No Hit
TACATATATGTACATATATGTACATATATGTACATATATGTACATATATG194390.13601386797089965No Hit
TATGTACATATATGTACATATATGTACATATATGTACATATATGTACATA189690.132725297676835No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT179650.1257003517720671No Hit
AATTCGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGGT173540.12142521038978306No Hit
AATTCGAACCTCTGTAACACTCAGACCACGCTGATGCCCAGCGCCTGTTT164880.11536584469901712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG77200.080.2156752
GTCGACG67400.079.4076461
AATTCGA59250.067.214991
ACGGCGC94450.066.9713445
GACGGCG97800.064.434564
CGGCGCG108400.058.6148346
CGACGGC114400.054.711383
AATTCCC107250.054.4764671
ATTCCCC113600.050.666772
GCGGTAC38000.050.488999
GGCGGTA40600.047.605128
ATTCGAG38900.047.4896432
TATCTCG45300.045.392982
TCGAACC41350.042.1480334
CGAACCT42750.040.4344945
CGAGGCG52200.036.935365
TTCGAGG52000.036.8035743
CGCGCCG214150.036.7010849
GGCGCGC219900.035.8046957
GCGCGCC220250.035.661938
Produced by FastQC (version 0.11.5)