FastQCFastQC Report
Tue 31 May 2016
SRR1051276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11810273
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT131105411.100962695781885No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT1419181.201648767983602No Hit
ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC347990.2946502591430359No Hit
ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT322570.27312662459199716No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT313440.26539606662775705No Hit
GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT282310.23903765814727568No Hit
ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT278410.2357354482830329No Hit
TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG261440.22136660177118683No Hit
ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT239430.20273028405016547No Hit
CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA220010.18628697236719252No Hit
TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA205580.1740687958694943No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT190230.16107163653202597No Hit
TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA176520.14946309877849562No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174690.14791360030373557No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA167090.14147852467085223No Hit
TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA160830.1361780544785036No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA140310.11880335026971858No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125520.1062803543999364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1162850.092.454161
ATATCTC1927300.091.481391
TATCTCG1941600.090.8903052
TCGACGG1202300.090.107952
ATCTCGA1985850.088.6511463
CGACGGC1223400.088.546243
GACGGCG1223750.088.471334
ACGGCGC1222850.088.46335
CGGCGCG1233900.088.0138556
TCTCGAG2004850.086.660924
CTCGAGG2036900.085.229825
TCGAGGG2051650.084.689396
CGAGGGC2064600.084.383597
AGGGCGC2089050.083.672429
GAGGGCG2091300.083.512188
TCGCGAG13750.070.606194
TATCGCG14350.068.62982
ATCGCGA14800.063.704753
TCTCGAC39550.057.3550834
CTCGACG39300.057.0020375