FastQCFastQC Report
Tue 31 May 2016
SRR1051276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11810273
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850200.7198817504049229No Hit
ATATGTATACATATGTATACATATGTATACATATGTATACATATGTATAC418900.35469120823879347No Hit
ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT398220.33718102875352673No Hit
ACATATGTATACATATGTATACATATGTATACATATGTATACATATGTAT343930.2912125740023114No Hit
GTATACATATGTATACATATGTATACATATGTATACATATGTATACATAT323350.27378706656484575No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT317400.26874907972068046No Hit
ATGTATACATATGTATACATATGTATACATATGTATACATATGTATACAT307590.26044275183139287No Hit
TATACATATGTATACATATGTATACATATGTATACATATGTATACATATG283880.2403670092977529No Hit
CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA276630.23422828583217337No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT238580.20201057164385616No Hit
TATGTATACATATGTATACATATGTATACATATGTATACATATGTATACA215060.18209570600103825No Hit
TACATATGTATACATATGTATACATATGTATACATATGTATACATATGTA198720.16826029339033907No Hit
TGTATACATATGTATACATATGTATACATATGTATACATATGTATACATA176570.1495054348023962No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119960.10157258854219542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG38500.078.752871
TCGACGG46150.077.3837662
ACGGCGC62600.058.6405265
GACGGCG62500.057.8987924
CGACGGC64350.055.791473
CGGCGCG83050.044.029136
ATCTCGA94150.038.7991071
TCTCGAG119150.032.4439772
CTCGAGG130200.030.0545863
TCGAGGG131900.029.883254
GGGCGCG135150.029.7264698
TTCGACG10550.028.9691121
CGAGGGC142000.027.9917345
AGGGCGC144950.027.749447
GAGGGCG152850.026.0045346
TATCTCG119050.024.8520582
CGCGCCG204950.024.3760999
GGCGCGC215000.023.177
GCGCGCC221200.022.5849768
ATATCTC139300.020.4076771
Produced by FastQC (version 0.11.5)